Evaluation of dot-blot test for serological diagnosis of bovine brucellosis

• Autor(a) principal: Bastos,Carla Resende
• Data de Publicação: 2018
• Outros Autores: Mathias,Luis Antonio, Jusi,Márcia Mariza Gomes, Santos,Renata Ferreira dos, Silva,Glaucenyra Cecília Pinheiro da, André,Marcos Rogério, Machado,Rosangela Zacarias, Bürger,Karina Paes
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Brazilian Journal of Microbiology
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000300564
• topic: Brucella abortus; Animal health; Zoonoses; 2-Mercaptoethanol; Complement fixation test

Abstract The objective of this study was to standardize and validate the dot-blot test for the serological diagnosis of bovine brucellosis, compare the results with those found in the 2-mercaptoethanol (2-ME) and complement fixation test (CF), and estimate the relative sensitivity and specificity of the dot-blot compared to these tests. Fifty bovine blood serum samples were used for the test standardization, and 1315 samples were used for evaluation and comparison between the tests; the results were compared using the Kappa indicator. At the end of standardization, it was established as optimal for the antigen obtained from Brucella abortus B19 after passing through a microorganism rupture process, the blood serum samples diluted at 1:100, and the conjugate at 1:30,000. The comparison of the dot-blot results with 2-ME showed Kappa index of 0.9939, sensitivity of 99.48%, and specificity 99.91%, with CF, Kappa index of 0.8226, sensitivity 100% and specificity 95.32%. Using the combination of the test results 2-ME and CF to establish the true condition of the animal, the dot-blot showed relative sensitivity of 100%, and relative specificity of 99.91%. The evaluated test proved to be effective and reliable, besides being easy to handle and interpret the results.