Antiviral activity of a Bacillus sp: P34 peptide against pathogenic viruses of domestic animals
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300043 |
Resumo: | P34 is an antimicrobial peptide produced by a Bacillus sp. strain isolated from the intestinal contents of a fish in the Brazilian Amazon basin with reported antibacterial activity. The aim of this work was to evaluate the peptide P34 for its in vitro antiviral properties against canine adenovirus type 2 (CAV-2), canine coronavirus (CCoV), canine distemper virus (CDV), canine parvovirus type 2 (CPV-2), equine arteritis virus (EAV), equine influenza virus (EIV), feline calicivirus (FCV) and feline herpesvirus type 1 (FHV-1). The results showed that the peptide P34 exhibited antiviral activity against EAV and FHV-1. The peptide P34 inhibited the replication of EAV by 99.9% and FHV-1 by 94.4%. Virucidal activity was detected only against EAV. When P34 and EAV were incubated for 6 h at 37 °C the viral titer reduced from 10(4.5) TCID50 to 10(2.75) TCID50, showing a percent of inhibition of 98.6%. In conclusion, our results demonstrated that P34 inhibited EAV and FHV-1 replication in infected cell cultures and it showed virucidal activity against EAV. Since there is documented resistance to the current drugs used against herpesviruses and there is no treatment for equine viral arteritis, it is advisable to search for new antiviral compounds to overcome these infections. |
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Brazilian Journal of Microbiology |
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Antiviral activity of a Bacillus sp: P34 peptide against pathogenic viruses of domestic animalsantimicrobial peptidesantiviral activityherpesvirusequine viral arteritisP34 is an antimicrobial peptide produced by a Bacillus sp. strain isolated from the intestinal contents of a fish in the Brazilian Amazon basin with reported antibacterial activity. The aim of this work was to evaluate the peptide P34 for its in vitro antiviral properties against canine adenovirus type 2 (CAV-2), canine coronavirus (CCoV), canine distemper virus (CDV), canine parvovirus type 2 (CPV-2), equine arteritis virus (EAV), equine influenza virus (EIV), feline calicivirus (FCV) and feline herpesvirus type 1 (FHV-1). The results showed that the peptide P34 exhibited antiviral activity against EAV and FHV-1. The peptide P34 inhibited the replication of EAV by 99.9% and FHV-1 by 94.4%. Virucidal activity was detected only against EAV. When P34 and EAV were incubated for 6 h at 37 °C the viral titer reduced from 10(4.5) TCID50 to 10(2.75) TCID50, showing a percent of inhibition of 98.6%. In conclusion, our results demonstrated that P34 inhibited EAV and FHV-1 replication in infected cell cultures and it showed virucidal activity against EAV. Since there is documented resistance to the current drugs used against herpesviruses and there is no treatment for equine viral arteritis, it is advisable to search for new antiviral compounds to overcome these infections.Sociedade Brasileira de Microbiologia2014-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300043Brazilian Journal of Microbiology v.45 n.3 2014reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822014000300043info:eu-repo/semantics/openAccessSilva,Débora Scopel eCastro,Clarissa Caetano deSilva,Fábio da Silva eSant'anna,VoltaireVargas,Gilberto D'AvilaLima,Marcelo deFischer,GefersonBrandelli,AdrianoMotta,Amanda de Souza daHübner,Silvia de Oliveiraeng2014-11-04T00:00:00Zoai:scielo:S1517-83822014000300043Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2014-11-04T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Antiviral activity of a Bacillus sp: P34 peptide against pathogenic viruses of domestic animals |
title |
Antiviral activity of a Bacillus sp: P34 peptide against pathogenic viruses of domestic animals |
spellingShingle |
Antiviral activity of a Bacillus sp: P34 peptide against pathogenic viruses of domestic animals Silva,Débora Scopel e antimicrobial peptides antiviral activity herpesvirus equine viral arteritis |
title_short |
Antiviral activity of a Bacillus sp: P34 peptide against pathogenic viruses of domestic animals |
title_full |
Antiviral activity of a Bacillus sp: P34 peptide against pathogenic viruses of domestic animals |
title_fullStr |
Antiviral activity of a Bacillus sp: P34 peptide against pathogenic viruses of domestic animals |
title_full_unstemmed |
Antiviral activity of a Bacillus sp: P34 peptide against pathogenic viruses of domestic animals |
title_sort |
Antiviral activity of a Bacillus sp: P34 peptide against pathogenic viruses of domestic animals |
author |
Silva,Débora Scopel e |
author_facet |
Silva,Débora Scopel e Castro,Clarissa Caetano de Silva,Fábio da Silva e Sant'anna,Voltaire Vargas,Gilberto D'Avila Lima,Marcelo de Fischer,Geferson Brandelli,Adriano Motta,Amanda de Souza da Hübner,Silvia de Oliveira |
author_role |
author |
author2 |
Castro,Clarissa Caetano de Silva,Fábio da Silva e Sant'anna,Voltaire Vargas,Gilberto D'Avila Lima,Marcelo de Fischer,Geferson Brandelli,Adriano Motta,Amanda de Souza da Hübner,Silvia de Oliveira |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Silva,Débora Scopel e Castro,Clarissa Caetano de Silva,Fábio da Silva e Sant'anna,Voltaire Vargas,Gilberto D'Avila Lima,Marcelo de Fischer,Geferson Brandelli,Adriano Motta,Amanda de Souza da Hübner,Silvia de Oliveira |
dc.subject.por.fl_str_mv |
antimicrobial peptides antiviral activity herpesvirus equine viral arteritis |
topic |
antimicrobial peptides antiviral activity herpesvirus equine viral arteritis |
description |
P34 is an antimicrobial peptide produced by a Bacillus sp. strain isolated from the intestinal contents of a fish in the Brazilian Amazon basin with reported antibacterial activity. The aim of this work was to evaluate the peptide P34 for its in vitro antiviral properties against canine adenovirus type 2 (CAV-2), canine coronavirus (CCoV), canine distemper virus (CDV), canine parvovirus type 2 (CPV-2), equine arteritis virus (EAV), equine influenza virus (EIV), feline calicivirus (FCV) and feline herpesvirus type 1 (FHV-1). The results showed that the peptide P34 exhibited antiviral activity against EAV and FHV-1. The peptide P34 inhibited the replication of EAV by 99.9% and FHV-1 by 94.4%. Virucidal activity was detected only against EAV. When P34 and EAV were incubated for 6 h at 37 °C the viral titer reduced from 10(4.5) TCID50 to 10(2.75) TCID50, showing a percent of inhibition of 98.6%. In conclusion, our results demonstrated that P34 inhibited EAV and FHV-1 replication in infected cell cultures and it showed virucidal activity against EAV. Since there is documented resistance to the current drugs used against herpesviruses and there is no treatment for equine viral arteritis, it is advisable to search for new antiviral compounds to overcome these infections. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300043 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300043 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-83822014000300043 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.45 n.3 2014 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122206640406528 |