Production and properties of the cellulase-free xylanase from Thermomyces lanuginosus IOC-4145
Autor(a) principal: | |
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Data de Publicação: | 2002 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000400011 |
Resumo: | In recent years, xylanases have expanded their use in many processing industries, such as pulp and paper, food and textile. Thermomyces lanuginosus IOC-4145 was able to produce a very high level of cellulase-free xylanase in shaken cultures using corncob as substrate (500 U/mL). An optimization of the medium composition in submerged fermentation was carried out aiming at a low cost medium composition for enzyme production. Statistical experiment design was employed for this purpose, pointing out corncob as the most important parameter, which affects enzyme production. Additionally, the influence of several chemicals on xylanase activity was investigated in the crude extract. A slight stimulation of the enzyme (5-15%) was achieved with NaCl and urea, both at 3 and 5 mM of concentration. On the other hand, dithiothreitol and beta-mercaptoethanol at a molarity of 5mM have caused a strong stimulation of the enzyme (40-53%). The crude xylanase displayed appreciable thermostability, retaining almost 50% of activity during 24 hours of incubation at 50ºC; about 50% of activity was present at 60ºC even after 4 hours of incubation. The enzyme also exhibited good storage stability at -20ºC without any stabilizing agent. |
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Brazilian Journal of Microbiology |
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Production and properties of the cellulase-free xylanase from Thermomyces lanuginosus IOC-4145XylanasesThermomyces lanuginosuscorncobsubmerged fermentationmedium optimizationIn recent years, xylanases have expanded their use in many processing industries, such as pulp and paper, food and textile. Thermomyces lanuginosus IOC-4145 was able to produce a very high level of cellulase-free xylanase in shaken cultures using corncob as substrate (500 U/mL). An optimization of the medium composition in submerged fermentation was carried out aiming at a low cost medium composition for enzyme production. Statistical experiment design was employed for this purpose, pointing out corncob as the most important parameter, which affects enzyme production. Additionally, the influence of several chemicals on xylanase activity was investigated in the crude extract. A slight stimulation of the enzyme (5-15%) was achieved with NaCl and urea, both at 3 and 5 mM of concentration. On the other hand, dithiothreitol and beta-mercaptoethanol at a molarity of 5mM have caused a strong stimulation of the enzyme (40-53%). The crude xylanase displayed appreciable thermostability, retaining almost 50% of activity during 24 hours of incubation at 50ºC; about 50% of activity was present at 60ºC even after 4 hours of incubation. The enzyme also exhibited good storage stability at -20ºC without any stabilizing agent.Sociedade Brasileira de Microbiologia2002-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000400011Brazilian Journal of Microbiology v.33 n.4 2002reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822002000400011info:eu-repo/semantics/openAccessDamaso,Mônica Caramez TrichesAndrade,Carolina M.M. CarvalhoPereira Jr,Neieng2014-04-28T00:00:00Zoai:scielo:S1517-83822002000400011Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2014-04-28T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Production and properties of the cellulase-free xylanase from Thermomyces lanuginosus IOC-4145 |
title |
Production and properties of the cellulase-free xylanase from Thermomyces lanuginosus IOC-4145 |
spellingShingle |
Production and properties of the cellulase-free xylanase from Thermomyces lanuginosus IOC-4145 Damaso,Mônica Caramez Triches Xylanases Thermomyces lanuginosus corncob submerged fermentation medium optimization |
title_short |
Production and properties of the cellulase-free xylanase from Thermomyces lanuginosus IOC-4145 |
title_full |
Production and properties of the cellulase-free xylanase from Thermomyces lanuginosus IOC-4145 |
title_fullStr |
Production and properties of the cellulase-free xylanase from Thermomyces lanuginosus IOC-4145 |
title_full_unstemmed |
Production and properties of the cellulase-free xylanase from Thermomyces lanuginosus IOC-4145 |
title_sort |
Production and properties of the cellulase-free xylanase from Thermomyces lanuginosus IOC-4145 |
author |
Damaso,Mônica Caramez Triches |
author_facet |
Damaso,Mônica Caramez Triches Andrade,Carolina M.M. Carvalho Pereira Jr,Nei |
author_role |
author |
author2 |
Andrade,Carolina M.M. Carvalho Pereira Jr,Nei |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Damaso,Mônica Caramez Triches Andrade,Carolina M.M. Carvalho Pereira Jr,Nei |
dc.subject.por.fl_str_mv |
Xylanases Thermomyces lanuginosus corncob submerged fermentation medium optimization |
topic |
Xylanases Thermomyces lanuginosus corncob submerged fermentation medium optimization |
description |
In recent years, xylanases have expanded their use in many processing industries, such as pulp and paper, food and textile. Thermomyces lanuginosus IOC-4145 was able to produce a very high level of cellulase-free xylanase in shaken cultures using corncob as substrate (500 U/mL). An optimization of the medium composition in submerged fermentation was carried out aiming at a low cost medium composition for enzyme production. Statistical experiment design was employed for this purpose, pointing out corncob as the most important parameter, which affects enzyme production. Additionally, the influence of several chemicals on xylanase activity was investigated in the crude extract. A slight stimulation of the enzyme (5-15%) was achieved with NaCl and urea, both at 3 and 5 mM of concentration. On the other hand, dithiothreitol and beta-mercaptoethanol at a molarity of 5mM have caused a strong stimulation of the enzyme (40-53%). The crude xylanase displayed appreciable thermostability, retaining almost 50% of activity during 24 hours of incubation at 50ºC; about 50% of activity was present at 60ºC even after 4 hours of incubation. The enzyme also exhibited good storage stability at -20ºC without any stabilizing agent. |
publishDate |
2002 |
dc.date.none.fl_str_mv |
2002-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000400011 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000400011 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-83822002000400011 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.33 n.4 2002 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122199334977536 |