High levels of β-xylosidase in Thermomyces lanuginosus: potential use for saccharification
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300680 |
Resumo: | ABSTRACT A new strain of Thermomyces lanuginosus was isolated from the Atlantic Forest biome, and its β-xylosidases optimization in response to agro-industrial residues was performed. Using statistical approach as a strategy for optimization, the induction of β-xylosidases activity was evaluated in residual corn straw, and improved so that the optimum condition achieved high β-xylosidases activities 1003 U/mL. According our known, this study is the first to show so high levels of β-xylosidases activities induction. In addition, the application of an experimental design with this microorganism to induce β-xylosidases has not been reported until the present work. The optimal conditions for the crude enzyme extract were pH 5.5 and 60 °C showing better thermostability at 55 °C. The saccharification ability of β-xylosidase in the presence of hemicellulose obtained from corn straw raw and xylan from beechwood substrates showed a xylo-oligosaccharide to xylose conversion yield of 80 and 50%, respectively, at 50 °C. Our data strongly indicated that the β-xylosidases activities was not subjected to the effects of potential enzyme inhibitors often produced during fermentation process. These data suggest the application of this enzyme studied for saccharification of hemicellulose, an abundant residue in the American continents, thus providing an interesting alternative for future tests for energy production. |
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High levels of β-xylosidase in Thermomyces lanuginosus: potential use for saccharificationExperimental designSaccharificationβ-XylosidaseThermomyces lanuginosusAtlantic ForestABSTRACT A new strain of Thermomyces lanuginosus was isolated from the Atlantic Forest biome, and its β-xylosidases optimization in response to agro-industrial residues was performed. Using statistical approach as a strategy for optimization, the induction of β-xylosidases activity was evaluated in residual corn straw, and improved so that the optimum condition achieved high β-xylosidases activities 1003 U/mL. According our known, this study is the first to show so high levels of β-xylosidases activities induction. In addition, the application of an experimental design with this microorganism to induce β-xylosidases has not been reported until the present work. The optimal conditions for the crude enzyme extract were pH 5.5 and 60 °C showing better thermostability at 55 °C. The saccharification ability of β-xylosidase in the presence of hemicellulose obtained from corn straw raw and xylan from beechwood substrates showed a xylo-oligosaccharide to xylose conversion yield of 80 and 50%, respectively, at 50 °C. Our data strongly indicated that the β-xylosidases activities was not subjected to the effects of potential enzyme inhibitors often produced during fermentation process. These data suggest the application of this enzyme studied for saccharification of hemicellulose, an abundant residue in the American continents, thus providing an interesting alternative for future tests for energy production.Sociedade Brasileira de Microbiologia2016-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300680Brazilian Journal of Microbiology v.47 n.3 2016reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1016/j.bjm.2016.04.028info:eu-repo/semantics/openAccessCorrêa,Juliana MoçoChristi,DivairTorre,Carla Lieko DellaHenn,CarolineConceição-Silva,José Luis daKadowaki,Marina KimikoSimão,Rita de Cássia Garciaeng2016-08-02T00:00:00Zoai:scielo:S1517-83822016000300680Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2016-08-02T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
High levels of β-xylosidase in Thermomyces lanuginosus: potential use for saccharification |
title |
High levels of β-xylosidase in Thermomyces lanuginosus: potential use for saccharification |
spellingShingle |
High levels of β-xylosidase in Thermomyces lanuginosus: potential use for saccharification Corrêa,Juliana Moço Experimental design Saccharification β-Xylosidase Thermomyces lanuginosus Atlantic Forest |
title_short |
High levels of β-xylosidase in Thermomyces lanuginosus: potential use for saccharification |
title_full |
High levels of β-xylosidase in Thermomyces lanuginosus: potential use for saccharification |
title_fullStr |
High levels of β-xylosidase in Thermomyces lanuginosus: potential use for saccharification |
title_full_unstemmed |
High levels of β-xylosidase in Thermomyces lanuginosus: potential use for saccharification |
title_sort |
High levels of β-xylosidase in Thermomyces lanuginosus: potential use for saccharification |
author |
Corrêa,Juliana Moço |
author_facet |
Corrêa,Juliana Moço Christi,Divair Torre,Carla Lieko Della Henn,Caroline Conceição-Silva,José Luis da Kadowaki,Marina Kimiko Simão,Rita de Cássia Garcia |
author_role |
author |
author2 |
Christi,Divair Torre,Carla Lieko Della Henn,Caroline Conceição-Silva,José Luis da Kadowaki,Marina Kimiko Simão,Rita de Cássia Garcia |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Corrêa,Juliana Moço Christi,Divair Torre,Carla Lieko Della Henn,Caroline Conceição-Silva,José Luis da Kadowaki,Marina Kimiko Simão,Rita de Cássia Garcia |
dc.subject.por.fl_str_mv |
Experimental design Saccharification β-Xylosidase Thermomyces lanuginosus Atlantic Forest |
topic |
Experimental design Saccharification β-Xylosidase Thermomyces lanuginosus Atlantic Forest |
description |
ABSTRACT A new strain of Thermomyces lanuginosus was isolated from the Atlantic Forest biome, and its β-xylosidases optimization in response to agro-industrial residues was performed. Using statistical approach as a strategy for optimization, the induction of β-xylosidases activity was evaluated in residual corn straw, and improved so that the optimum condition achieved high β-xylosidases activities 1003 U/mL. According our known, this study is the first to show so high levels of β-xylosidases activities induction. In addition, the application of an experimental design with this microorganism to induce β-xylosidases has not been reported until the present work. The optimal conditions for the crude enzyme extract were pH 5.5 and 60 °C showing better thermostability at 55 °C. The saccharification ability of β-xylosidase in the presence of hemicellulose obtained from corn straw raw and xylan from beechwood substrates showed a xylo-oligosaccharide to xylose conversion yield of 80 and 50%, respectively, at 50 °C. Our data strongly indicated that the β-xylosidases activities was not subjected to the effects of potential enzyme inhibitors often produced during fermentation process. These data suggest the application of this enzyme studied for saccharification of hemicellulose, an abundant residue in the American continents, thus providing an interesting alternative for future tests for energy production. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300680 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300680 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1016/j.bjm.2016.04.028 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.47 n.3 2016 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122208446054400 |