Seroprevalence of Borrelia burgdorferi in occupationally exposed persons in the Belgrade area, Serbia

Detalhes bibliográficos
Autor(a) principal: Jovanovic,Dragutin
Data de Publicação: 2015
Outros Autores: Atanasievska,Sonja, Protic-Djokic,Vesna, Rakic,Uros, Lukac-Radoncic,Elvira, Ristanovic,Elizabeta
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300807
Resumo: Lyme disease (LD) is a natural focal zoonotic disease caused by Borrelia burgdorferi, which is mainly transmitted through infected Ixodes ricinus tick bites. The presence and abundance of ticks in various habitats, the infectivity rate, as well as prolonged human exposure to ticks are factors that may affect the infection risk as well as the incidence of LD. In recent years, 20% to 25% of ticks infected with different borrelial species, as well as about 5,300 citizens with LD, have been registered in the Belgrade area. Many of the patients reported tick bites in city’s grassy areas. The aim of this study was to assess the seroprevalence of B. burgdorferi in high-risk groups (forestry workers and soldiers) in the Belgrade area, and to compare the results with healthy blood donors. A two-step algorithm consisting of ELISA and Western blot tests was used in the study. Immunoreactivity profiles were also compared between the groups. The results obtained showed the seroprevalence to be 11.76% in the group of forestry workers, 17.14% in the group of soldiers infected by tick bites and 8.57% in the population of healthy blood donors. The highest IgM reactivity was detected against the OspC protein, while IgG antibodies showed high reactivity against VlsE, p19, p41, OspC, OspA and p17. Further investigations in this field are necessary in humans and animals in order to improve protective and preventive measures against LD.
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spelling Seroprevalence of Borrelia burgdorferi in occupationally exposed persons in the Belgrade area, SerbiaBorrelia burgdorferiLyme diseaseseroprevalenceforestry workerssoldiersLyme disease (LD) is a natural focal zoonotic disease caused by Borrelia burgdorferi, which is mainly transmitted through infected Ixodes ricinus tick bites. The presence and abundance of ticks in various habitats, the infectivity rate, as well as prolonged human exposure to ticks are factors that may affect the infection risk as well as the incidence of LD. In recent years, 20% to 25% of ticks infected with different borrelial species, as well as about 5,300 citizens with LD, have been registered in the Belgrade area. Many of the patients reported tick bites in city’s grassy areas. The aim of this study was to assess the seroprevalence of B. burgdorferi in high-risk groups (forestry workers and soldiers) in the Belgrade area, and to compare the results with healthy blood donors. A two-step algorithm consisting of ELISA and Western blot tests was used in the study. Immunoreactivity profiles were also compared between the groups. The results obtained showed the seroprevalence to be 11.76% in the group of forestry workers, 17.14% in the group of soldiers infected by tick bites and 8.57% in the population of healthy blood donors. The highest IgM reactivity was detected against the OspC protein, while IgG antibodies showed high reactivity against VlsE, p19, p41, OspC, OspA and p17. Further investigations in this field are necessary in humans and animals in order to improve protective and preventive measures against LD.Sociedade Brasileira de Microbiologia2015-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300807Brazilian Journal of Microbiology v.46 n.3 2015reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-838246320140698info:eu-repo/semantics/openAccessJovanovic,DragutinAtanasievska,SonjaProtic-Djokic,VesnaRakic,UrosLukac-Radoncic,ElviraRistanovic,Elizabetaeng2015-08-31T00:00:00Zoai:scielo:S1517-83822015000300807Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2015-08-31T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Seroprevalence of Borrelia burgdorferi in occupationally exposed persons in the Belgrade area, Serbia
title Seroprevalence of Borrelia burgdorferi in occupationally exposed persons in the Belgrade area, Serbia
spellingShingle Seroprevalence of Borrelia burgdorferi in occupationally exposed persons in the Belgrade area, Serbia
Jovanovic,Dragutin
Borrelia burgdorferi
Lyme disease
seroprevalence
forestry workers
soldiers
title_short Seroprevalence of Borrelia burgdorferi in occupationally exposed persons in the Belgrade area, Serbia
title_full Seroprevalence of Borrelia burgdorferi in occupationally exposed persons in the Belgrade area, Serbia
title_fullStr Seroprevalence of Borrelia burgdorferi in occupationally exposed persons in the Belgrade area, Serbia
title_full_unstemmed Seroprevalence of Borrelia burgdorferi in occupationally exposed persons in the Belgrade area, Serbia
title_sort Seroprevalence of Borrelia burgdorferi in occupationally exposed persons in the Belgrade area, Serbia
author Jovanovic,Dragutin
author_facet Jovanovic,Dragutin
Atanasievska,Sonja
Protic-Djokic,Vesna
Rakic,Uros
Lukac-Radoncic,Elvira
Ristanovic,Elizabeta
author_role author
author2 Atanasievska,Sonja
Protic-Djokic,Vesna
Rakic,Uros
Lukac-Radoncic,Elvira
Ristanovic,Elizabeta
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Jovanovic,Dragutin
Atanasievska,Sonja
Protic-Djokic,Vesna
Rakic,Uros
Lukac-Radoncic,Elvira
Ristanovic,Elizabeta
dc.subject.por.fl_str_mv Borrelia burgdorferi
Lyme disease
seroprevalence
forestry workers
soldiers
topic Borrelia burgdorferi
Lyme disease
seroprevalence
forestry workers
soldiers
description Lyme disease (LD) is a natural focal zoonotic disease caused by Borrelia burgdorferi, which is mainly transmitted through infected Ixodes ricinus tick bites. The presence and abundance of ticks in various habitats, the infectivity rate, as well as prolonged human exposure to ticks are factors that may affect the infection risk as well as the incidence of LD. In recent years, 20% to 25% of ticks infected with different borrelial species, as well as about 5,300 citizens with LD, have been registered in the Belgrade area. Many of the patients reported tick bites in city’s grassy areas. The aim of this study was to assess the seroprevalence of B. burgdorferi in high-risk groups (forestry workers and soldiers) in the Belgrade area, and to compare the results with healthy blood donors. A two-step algorithm consisting of ELISA and Western blot tests was used in the study. Immunoreactivity profiles were also compared between the groups. The results obtained showed the seroprevalence to be 11.76% in the group of forestry workers, 17.14% in the group of soldiers infected by tick bites and 8.57% in the population of healthy blood donors. The highest IgM reactivity was detected against the OspC protein, while IgG antibodies showed high reactivity against VlsE, p19, p41, OspC, OspA and p17. Further investigations in this field are necessary in humans and animals in order to improve protective and preventive measures against LD.
publishDate 2015
dc.date.none.fl_str_mv 2015-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300807
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300807
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-838246320140698
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.46 n.3 2015
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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