Nitrogen consumption during batch cultivation of Neisseria meningitidis (serogroup C) in Frantz medium
Autor(a) principal: | |
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Data de Publicação: | 2001 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000400010 |
Resumo: | Capsular polysaccharide, extracted from microorganism cultivations, is the principal antigen for elaboration of vaccine against the disease caused by Neisseria meningitidis serogroup C. The final protein content allowed in this vaccine is 1%. In order to find a relationship between nitrogen consumption and cell growth, including polysaccharide production, and cell nitrogen content, cultivations were carried out in an 80 liters bioreactor (total capacity), under the following conditions: Frantz medium; temperature of 35ºC; air flow of 5 L/min (0.125 vvm); agitation frequency of 120 rpm and vessel pressure of 6 psi (kLa = 0.07 min-1). Concentrations of biomass, total polysaccharide, cellular nitrogen, residual organic and inorganic nitrogen in the medium were measured during cultivation. From five cultivations carried out under the same conditions, a mean cell nitrogen percentage of 12.6% (w/w) in respect to the dry biomass was found. The inorganic nitrogen in the medium did not change significantly along the cultivation time, whereas the organic nitrogen consumption was linearly related to cell growth, with constant yield factors (average of 8.44). Polysaccharide production kinetics followed the cell growth kinetics until the beginning of the stationary growth phase. A supplemental polysaccharide production was observed until the end of cultivation, but without cell nitrogen absorption. Thus, the results indicate that polysaccharide is produced in two phases, being the first one biomass formation followed by non-associated to growth. |
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Brazilian Journal of Microbiology |
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Nitrogen consumption during batch cultivation of Neisseria meningitidis (serogroup C) in Frantz mediumN. meningitidisvaccinepolysaccharidenitrogenCapsular polysaccharide, extracted from microorganism cultivations, is the principal antigen for elaboration of vaccine against the disease caused by Neisseria meningitidis serogroup C. The final protein content allowed in this vaccine is 1%. In order to find a relationship between nitrogen consumption and cell growth, including polysaccharide production, and cell nitrogen content, cultivations were carried out in an 80 liters bioreactor (total capacity), under the following conditions: Frantz medium; temperature of 35ºC; air flow of 5 L/min (0.125 vvm); agitation frequency of 120 rpm and vessel pressure of 6 psi (kLa = 0.07 min-1). Concentrations of biomass, total polysaccharide, cellular nitrogen, residual organic and inorganic nitrogen in the medium were measured during cultivation. From five cultivations carried out under the same conditions, a mean cell nitrogen percentage of 12.6% (w/w) in respect to the dry biomass was found. The inorganic nitrogen in the medium did not change significantly along the cultivation time, whereas the organic nitrogen consumption was linearly related to cell growth, with constant yield factors (average of 8.44). Polysaccharide production kinetics followed the cell growth kinetics until the beginning of the stationary growth phase. A supplemental polysaccharide production was observed until the end of cultivation, but without cell nitrogen absorption. Thus, the results indicate that polysaccharide is produced in two phases, being the first one biomass formation followed by non-associated to growth.Sociedade Brasileira de Microbiologia2001-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000400010Brazilian Journal of Microbiology v.32 n.4 2001reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822001000400010info:eu-repo/semantics/openAccessBaruque-Ramos,JúliaHiss,HaroldoVicentin,Márcio AlbertoPaz,Marcelo Fossa daPeixoto,ArgéliaLeal,Maria Betania BatistaSato,Rony AkioVassoler,Umbelina MacedoRaw,Isaíaseng2002-09-02T00:00:00Zoai:scielo:S1517-83822001000400010Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2002-09-02T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Nitrogen consumption during batch cultivation of Neisseria meningitidis (serogroup C) in Frantz medium |
title |
Nitrogen consumption during batch cultivation of Neisseria meningitidis (serogroup C) in Frantz medium |
spellingShingle |
Nitrogen consumption during batch cultivation of Neisseria meningitidis (serogroup C) in Frantz medium Baruque-Ramos,Júlia N. meningitidis vaccine polysaccharide nitrogen |
title_short |
Nitrogen consumption during batch cultivation of Neisseria meningitidis (serogroup C) in Frantz medium |
title_full |
Nitrogen consumption during batch cultivation of Neisseria meningitidis (serogroup C) in Frantz medium |
title_fullStr |
Nitrogen consumption during batch cultivation of Neisseria meningitidis (serogroup C) in Frantz medium |
title_full_unstemmed |
Nitrogen consumption during batch cultivation of Neisseria meningitidis (serogroup C) in Frantz medium |
title_sort |
Nitrogen consumption during batch cultivation of Neisseria meningitidis (serogroup C) in Frantz medium |
author |
Baruque-Ramos,Júlia |
author_facet |
Baruque-Ramos,Júlia Hiss,Haroldo Vicentin,Márcio Alberto Paz,Marcelo Fossa da Peixoto,Argélia Leal,Maria Betania Batista Sato,Rony Akio Vassoler,Umbelina Macedo Raw,Isaías |
author_role |
author |
author2 |
Hiss,Haroldo Vicentin,Márcio Alberto Paz,Marcelo Fossa da Peixoto,Argélia Leal,Maria Betania Batista Sato,Rony Akio Vassoler,Umbelina Macedo Raw,Isaías |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Baruque-Ramos,Júlia Hiss,Haroldo Vicentin,Márcio Alberto Paz,Marcelo Fossa da Peixoto,Argélia Leal,Maria Betania Batista Sato,Rony Akio Vassoler,Umbelina Macedo Raw,Isaías |
dc.subject.por.fl_str_mv |
N. meningitidis vaccine polysaccharide nitrogen |
topic |
N. meningitidis vaccine polysaccharide nitrogen |
description |
Capsular polysaccharide, extracted from microorganism cultivations, is the principal antigen for elaboration of vaccine against the disease caused by Neisseria meningitidis serogroup C. The final protein content allowed in this vaccine is 1%. In order to find a relationship between nitrogen consumption and cell growth, including polysaccharide production, and cell nitrogen content, cultivations were carried out in an 80 liters bioreactor (total capacity), under the following conditions: Frantz medium; temperature of 35ºC; air flow of 5 L/min (0.125 vvm); agitation frequency of 120 rpm and vessel pressure of 6 psi (kLa = 0.07 min-1). Concentrations of biomass, total polysaccharide, cellular nitrogen, residual organic and inorganic nitrogen in the medium were measured during cultivation. From five cultivations carried out under the same conditions, a mean cell nitrogen percentage of 12.6% (w/w) in respect to the dry biomass was found. The inorganic nitrogen in the medium did not change significantly along the cultivation time, whereas the organic nitrogen consumption was linearly related to cell growth, with constant yield factors (average of 8.44). Polysaccharide production kinetics followed the cell growth kinetics until the beginning of the stationary growth phase. A supplemental polysaccharide production was observed until the end of cultivation, but without cell nitrogen absorption. Thus, the results indicate that polysaccharide is produced in two phases, being the first one biomass formation followed by non-associated to growth. |
publishDate |
2001 |
dc.date.none.fl_str_mv |
2001-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000400010 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000400010 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-83822001000400010 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.32 n.4 2001 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122199215439872 |