Phylogenetic characterization of the first Ungulate tetraparvovirus 2 detected in pigs in Brazil
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200513 |
Resumo: | Abstract Ungulate tetraparvovirus 2 (UTV2) , formerly known as porcine hokovirus due to its discovery in Hong Kong, is closely related to a Primate tetraparvovirus (human PARV-4) and Ungulate tetraparvovirus 1 (bovine hokovirus). Until now, UTV2 was detected in European, Asian and North American countries, but its occurrence in Latin America is still unknown. This study describes the first report of UTV2 in Brazil, as well as its phylogenetic characterization. Tissue samples (lymph node, lung, liver, spleen and kidney) of 240 piglets from eight different herds (30 animals each herd) were processed for DNA extraction. UTV2 DNA was detected by PCR and the entire VP1/VP2 gene was sequenced for phylogenetic analysis. All pigs from this study displayed postweaning multisystemic wasting syndrome (PMWS). UTV2 was detected in 55.3% of the samples distributed in the variety of porcine tissues investigated, as well as detected in almost all herds, with one exception. The phylogenetic analysis demonstrated that Brazilian UTV2 sequences were more closely related to sequences from Europe and United States. |
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Brazilian Journal of Microbiology |
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Phylogenetic characterization of the first Ungulate tetraparvovirus 2 detected in pigs in BrazilPorcineTetraparvovirusPhylogenyHokovirusDetectionUngulateAbstract Ungulate tetraparvovirus 2 (UTV2) , formerly known as porcine hokovirus due to its discovery in Hong Kong, is closely related to a Primate tetraparvovirus (human PARV-4) and Ungulate tetraparvovirus 1 (bovine hokovirus). Until now, UTV2 was detected in European, Asian and North American countries, but its occurrence in Latin America is still unknown. This study describes the first report of UTV2 in Brazil, as well as its phylogenetic characterization. Tissue samples (lymph node, lung, liver, spleen and kidney) of 240 piglets from eight different herds (30 animals each herd) were processed for DNA extraction. UTV2 DNA was detected by PCR and the entire VP1/VP2 gene was sequenced for phylogenetic analysis. All pigs from this study displayed postweaning multisystemic wasting syndrome (PMWS). UTV2 was detected in 55.3% of the samples distributed in the variety of porcine tissues investigated, as well as detected in almost all herds, with one exception. The phylogenetic analysis demonstrated that Brazilian UTV2 sequences were more closely related to sequences from Europe and United States.Sociedade Brasileira de Microbiologia2016-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200513Brazilian Journal of Microbiology v.47 n.2 2016reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1016/j.bjm.2016.01.025info:eu-repo/semantics/openAccessSouza,Carine KunzlerStreck,André FelipeGonçalves,Karla RatjePinto,Luciane DubinaRavazzolo,Ana PaulaBarcellos,David Emílio dos Santos Neves deCanal,Cláudio Wageckeng2016-05-03T00:00:00Zoai:scielo:S1517-83822016000200513Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2016-05-03T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Phylogenetic characterization of the first Ungulate tetraparvovirus 2 detected in pigs in Brazil |
title |
Phylogenetic characterization of the first Ungulate tetraparvovirus 2 detected in pigs in Brazil |
spellingShingle |
Phylogenetic characterization of the first Ungulate tetraparvovirus 2 detected in pigs in Brazil Souza,Carine Kunzler Porcine Tetraparvovirus Phylogeny Hokovirus Detection Ungulate |
title_short |
Phylogenetic characterization of the first Ungulate tetraparvovirus 2 detected in pigs in Brazil |
title_full |
Phylogenetic characterization of the first Ungulate tetraparvovirus 2 detected in pigs in Brazil |
title_fullStr |
Phylogenetic characterization of the first Ungulate tetraparvovirus 2 detected in pigs in Brazil |
title_full_unstemmed |
Phylogenetic characterization of the first Ungulate tetraparvovirus 2 detected in pigs in Brazil |
title_sort |
Phylogenetic characterization of the first Ungulate tetraparvovirus 2 detected in pigs in Brazil |
author |
Souza,Carine Kunzler |
author_facet |
Souza,Carine Kunzler Streck,André Felipe Gonçalves,Karla Ratje Pinto,Luciane Dubina Ravazzolo,Ana Paula Barcellos,David Emílio dos Santos Neves de Canal,Cláudio Wageck |
author_role |
author |
author2 |
Streck,André Felipe Gonçalves,Karla Ratje Pinto,Luciane Dubina Ravazzolo,Ana Paula Barcellos,David Emílio dos Santos Neves de Canal,Cláudio Wageck |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Souza,Carine Kunzler Streck,André Felipe Gonçalves,Karla Ratje Pinto,Luciane Dubina Ravazzolo,Ana Paula Barcellos,David Emílio dos Santos Neves de Canal,Cláudio Wageck |
dc.subject.por.fl_str_mv |
Porcine Tetraparvovirus Phylogeny Hokovirus Detection Ungulate |
topic |
Porcine Tetraparvovirus Phylogeny Hokovirus Detection Ungulate |
description |
Abstract Ungulate tetraparvovirus 2 (UTV2) , formerly known as porcine hokovirus due to its discovery in Hong Kong, is closely related to a Primate tetraparvovirus (human PARV-4) and Ungulate tetraparvovirus 1 (bovine hokovirus). Until now, UTV2 was detected in European, Asian and North American countries, but its occurrence in Latin America is still unknown. This study describes the first report of UTV2 in Brazil, as well as its phylogenetic characterization. Tissue samples (lymph node, lung, liver, spleen and kidney) of 240 piglets from eight different herds (30 animals each herd) were processed for DNA extraction. UTV2 DNA was detected by PCR and the entire VP1/VP2 gene was sequenced for phylogenetic analysis. All pigs from this study displayed postweaning multisystemic wasting syndrome (PMWS). UTV2 was detected in 55.3% of the samples distributed in the variety of porcine tissues investigated, as well as detected in almost all herds, with one exception. The phylogenetic analysis demonstrated that Brazilian UTV2 sequences were more closely related to sequences from Europe and United States. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200513 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200513 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1016/j.bjm.2016.01.025 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.47 n.2 2016 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122208405159936 |