Malignant Catarrhal Fever in Brazilian cattle presenting with neurological syndrome

Detalhes bibliográficos
Autor(a) principal: Martins,Maira de S.N.
Data de Publicação: 2017
Outros Autores: Castro,Alessandra M.M.G. de, Lima,Michele dos S., Pinto,Vivian da S.C., Silva,Thaís G. da, Fava,Claudia Del, Depes,Claudio Regis, Okuda,Liria H., Pituco,Edviges M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000200366
Resumo: Abstract Malignant Catarrhal Fever (MCF) was investigated in the central nervous system of cattle with neurological syndrome. Two-hundred-ninety samples were analyzed by histology, and molecular methods to detect ovine herpesvirus type 2 (OvHV-2) were optimized and validated. The qualitative polymerase chain reaction (qualitative PCR) analytical sensitivity was 101 DNA copies/µL and found 4.8% (14/290) positive for OvHV-2. The quantitative polymerase chain reaction (qPCR) analytical sensitivity was 100 DNA copy/µL and 5.9% (17/290) positivity, with 47.1% (8/17) of the positive samples presenting histological evidence of non-purulent meningo-encephalitis. The qualitative PCR products (422 bp of the ORF75 region) were sequenced and submitted to phylogenetic analysis. Identity matrices showed 100% similarity in OvHV-2 samples obtained in this study and those recovered from GenBank, corroborating other studies.
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spelling Malignant Catarrhal Fever in Brazilian cattle presenting with neurological syndromeOvine herpesvirus type 2 (OvHV-2)HistopathologyQualitative PCRQuantitative PCRPhylogenetic analysisAbstract Malignant Catarrhal Fever (MCF) was investigated in the central nervous system of cattle with neurological syndrome. Two-hundred-ninety samples were analyzed by histology, and molecular methods to detect ovine herpesvirus type 2 (OvHV-2) were optimized and validated. The qualitative polymerase chain reaction (qualitative PCR) analytical sensitivity was 101 DNA copies/µL and found 4.8% (14/290) positive for OvHV-2. The quantitative polymerase chain reaction (qPCR) analytical sensitivity was 100 DNA copy/µL and 5.9% (17/290) positivity, with 47.1% (8/17) of the positive samples presenting histological evidence of non-purulent meningo-encephalitis. The qualitative PCR products (422 bp of the ORF75 region) were sequenced and submitted to phylogenetic analysis. Identity matrices showed 100% similarity in OvHV-2 samples obtained in this study and those recovered from GenBank, corroborating other studies.Sociedade Brasileira de Microbiologia2017-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000200366Brazilian Journal of Microbiology v.48 n.2 2017reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1016/j.bjm.2016.10.021info:eu-repo/semantics/openAccessMartins,Maira de S.N.Castro,Alessandra M.M.G. deLima,Michele dos S.Pinto,Vivian da S.C.Silva,Thaís G. daFava,Claudia DelDepes,Claudio RegisOkuda,Liria H.Pituco,Edviges M.eng2017-05-11T00:00:00Zoai:scielo:S1517-83822017000200366Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2017-05-11T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Malignant Catarrhal Fever in Brazilian cattle presenting with neurological syndrome
title Malignant Catarrhal Fever in Brazilian cattle presenting with neurological syndrome
spellingShingle Malignant Catarrhal Fever in Brazilian cattle presenting with neurological syndrome
Martins,Maira de S.N.
Ovine herpesvirus type 2 (OvHV-2)
Histopathology
Qualitative PCR
Quantitative PCR
Phylogenetic analysis
title_short Malignant Catarrhal Fever in Brazilian cattle presenting with neurological syndrome
title_full Malignant Catarrhal Fever in Brazilian cattle presenting with neurological syndrome
title_fullStr Malignant Catarrhal Fever in Brazilian cattle presenting with neurological syndrome
title_full_unstemmed Malignant Catarrhal Fever in Brazilian cattle presenting with neurological syndrome
title_sort Malignant Catarrhal Fever in Brazilian cattle presenting with neurological syndrome
author Martins,Maira de S.N.
author_facet Martins,Maira de S.N.
Castro,Alessandra M.M.G. de
Lima,Michele dos S.
Pinto,Vivian da S.C.
Silva,Thaís G. da
Fava,Claudia Del
Depes,Claudio Regis
Okuda,Liria H.
Pituco,Edviges M.
author_role author
author2 Castro,Alessandra M.M.G. de
Lima,Michele dos S.
Pinto,Vivian da S.C.
Silva,Thaís G. da
Fava,Claudia Del
Depes,Claudio Regis
Okuda,Liria H.
Pituco,Edviges M.
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Martins,Maira de S.N.
Castro,Alessandra M.M.G. de
Lima,Michele dos S.
Pinto,Vivian da S.C.
Silva,Thaís G. da
Fava,Claudia Del
Depes,Claudio Regis
Okuda,Liria H.
Pituco,Edviges M.
dc.subject.por.fl_str_mv Ovine herpesvirus type 2 (OvHV-2)
Histopathology
Qualitative PCR
Quantitative PCR
Phylogenetic analysis
topic Ovine herpesvirus type 2 (OvHV-2)
Histopathology
Qualitative PCR
Quantitative PCR
Phylogenetic analysis
description Abstract Malignant Catarrhal Fever (MCF) was investigated in the central nervous system of cattle with neurological syndrome. Two-hundred-ninety samples were analyzed by histology, and molecular methods to detect ovine herpesvirus type 2 (OvHV-2) were optimized and validated. The qualitative polymerase chain reaction (qualitative PCR) analytical sensitivity was 101 DNA copies/µL and found 4.8% (14/290) positive for OvHV-2. The quantitative polymerase chain reaction (qPCR) analytical sensitivity was 100 DNA copy/µL and 5.9% (17/290) positivity, with 47.1% (8/17) of the positive samples presenting histological evidence of non-purulent meningo-encephalitis. The qualitative PCR products (422 bp of the ORF75 region) were sequenced and submitted to phylogenetic analysis. Identity matrices showed 100% similarity in OvHV-2 samples obtained in this study and those recovered from GenBank, corroborating other studies.
publishDate 2017
dc.date.none.fl_str_mv 2017-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000200366
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000200366
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1016/j.bjm.2016.10.021
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.48 n.2 2017
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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