Regulation of T lymphocyte subpopulations in specific pathogen-free chickens following experimental fowl adenovirus-Ⅷinfection

• Autor(a) principal: Wang,Chun-jie
• Data de Publicação: 2012
• Outros Autores: Yu,Siriguleng, Ao-ri-ge-le, Jia,De-gang, Yao,Hong-qiang, Zhao,Huai-ping, Lillehoj,H.S., Si-mu-ji-de, Postnikoff,Andrew CL, Xu,Si-ri-gu-leng
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Brazilian Journal of Microbiology
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400007
• topic: Cell mediated immune competency; Fowl adenovirus VIII; Immune system; Peripheral blood; T lymphocyte subpopulations

Two-day-old specific pathogen-free (SPF) chickens were divided into two groups. Group I was inoculated orally with fowl adenovirus VIII (FAV-VIII). Group II served as a negative control. Chickens were investigated at various days post-inoculation (dpi) by flow cytometric analysis for changes in T lymphocyte subpopulations in immune system and blood. In the thymus, CD3+ T lymphocytes were increased at 25 dpi, with significant increases in the FAV infected noted at 1, 12, 20dpi (p<0.05). This was accompanied by a corresponding increase of CD4+ and CD8+ T lymphocytes. In the spleen, CD3+ and CD4+ T lymphocytes were increased significantly at 30 dpi (p<0.01) whereas CD8+ and TCR γ δ+ T lymphocytes were decreased at 1 (p<0.05), 30 dpi (p<0.01). An increase of CD3+, CD4+ and CD8+ T lymphocytes was noticed in peripheral blood, and accompanied by a decrease of TCR γ δ+ T lymphocytes. These results demonstrated that infection with FAV-VIII causes significant fluctuations in T lymphocyte subpopulations in thymus, blood and spleen. It can be concluded that an infection with FAV-VIII has profound effects on the immune system, especially on cell mediated immune competency.