The inhibitory effect of calcium on Cylindrospermopsis raciborskii (cyanobacteria) metabolism

Detalhes bibliográficos
Autor(a) principal: Carneiro,Ronaldo Leal
Data de Publicação: 2011
Outros Autores: Alípio,Ana Carla Nascimento, Bisch,Paulo Mascarello, Azevedo,Sandra Maria Feliciano de Oliveira e, Pacheco,Ana Beatriz Furlanetto
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400042
Resumo: Cylindrospermopsis raciborskii (Woloszynska) Seenaya & Subba Raju is a freshwater cyanobacterium of worldwide distribution. In the North-eastern region of Brazil many eutrophic water reservoirs are characterized by the dominance of C. raciborskii, with recurrent occurrence of blooms. These water bodies have high conductivity due to a high ionic concentration, and are defined as hard (with high values of CaCO3). In this study, we investigated the long-term effect (12 days) of high calcium concentration (8 mM Ca2+) on C. raciborskii (T3 strain) growth, morphology, toxin content, and metabolism. Changes in protein expression profiles were investigated by proteomic analysis using 2D gel electrophoresis and mass spectrometry. A continued exposure to calcium had a pronounced effect on C. raciborskii (T3): it limited growth, decreased thricome length, increased chlorophyll-a content, altered toxin profile (although did not affect PST content, saxitoxin + neosaxitoxin), and inhibited the expression of proteins related to primary metabolism.
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spelling The inhibitory effect of calcium on Cylindrospermopsis raciborskii (cyanobacteria) metabolismcalciumCylindrospermopsisparalitic shellfish toxinproteomeCylindrospermopsis raciborskii (Woloszynska) Seenaya & Subba Raju is a freshwater cyanobacterium of worldwide distribution. In the North-eastern region of Brazil many eutrophic water reservoirs are characterized by the dominance of C. raciborskii, with recurrent occurrence of blooms. These water bodies have high conductivity due to a high ionic concentration, and are defined as hard (with high values of CaCO3). In this study, we investigated the long-term effect (12 days) of high calcium concentration (8 mM Ca2+) on C. raciborskii (T3 strain) growth, morphology, toxin content, and metabolism. Changes in protein expression profiles were investigated by proteomic analysis using 2D gel electrophoresis and mass spectrometry. A continued exposure to calcium had a pronounced effect on C. raciborskii (T3): it limited growth, decreased thricome length, increased chlorophyll-a content, altered toxin profile (although did not affect PST content, saxitoxin + neosaxitoxin), and inhibited the expression of proteins related to primary metabolism.Sociedade Brasileira de Microbiologia2011-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400042Brazilian Journal of Microbiology v.42 n.4 2011reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822011000400042info:eu-repo/semantics/openAccessCarneiro,Ronaldo LealAlípio,Ana Carla NascimentoBisch,Paulo MascarelloAzevedo,Sandra Maria Feliciano de Oliveira ePacheco,Ana Beatriz Furlanettoeng2015-06-30T00:00:00Zoai:scielo:S1517-83822011000400042Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2015-06-30T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv The inhibitory effect of calcium on Cylindrospermopsis raciborskii (cyanobacteria) metabolism
title The inhibitory effect of calcium on Cylindrospermopsis raciborskii (cyanobacteria) metabolism
spellingShingle The inhibitory effect of calcium on Cylindrospermopsis raciborskii (cyanobacteria) metabolism
Carneiro,Ronaldo Leal
calcium
Cylindrospermopsis
paralitic shellfish toxin
proteome
title_short The inhibitory effect of calcium on Cylindrospermopsis raciborskii (cyanobacteria) metabolism
title_full The inhibitory effect of calcium on Cylindrospermopsis raciborskii (cyanobacteria) metabolism
title_fullStr The inhibitory effect of calcium on Cylindrospermopsis raciborskii (cyanobacteria) metabolism
title_full_unstemmed The inhibitory effect of calcium on Cylindrospermopsis raciborskii (cyanobacteria) metabolism
title_sort The inhibitory effect of calcium on Cylindrospermopsis raciborskii (cyanobacteria) metabolism
author Carneiro,Ronaldo Leal
author_facet Carneiro,Ronaldo Leal
Alípio,Ana Carla Nascimento
Bisch,Paulo Mascarello
Azevedo,Sandra Maria Feliciano de Oliveira e
Pacheco,Ana Beatriz Furlanetto
author_role author
author2 Alípio,Ana Carla Nascimento
Bisch,Paulo Mascarello
Azevedo,Sandra Maria Feliciano de Oliveira e
Pacheco,Ana Beatriz Furlanetto
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Carneiro,Ronaldo Leal
Alípio,Ana Carla Nascimento
Bisch,Paulo Mascarello
Azevedo,Sandra Maria Feliciano de Oliveira e
Pacheco,Ana Beatriz Furlanetto
dc.subject.por.fl_str_mv calcium
Cylindrospermopsis
paralitic shellfish toxin
proteome
topic calcium
Cylindrospermopsis
paralitic shellfish toxin
proteome
description Cylindrospermopsis raciborskii (Woloszynska) Seenaya & Subba Raju is a freshwater cyanobacterium of worldwide distribution. In the North-eastern region of Brazil many eutrophic water reservoirs are characterized by the dominance of C. raciborskii, with recurrent occurrence of blooms. These water bodies have high conductivity due to a high ionic concentration, and are defined as hard (with high values of CaCO3). In this study, we investigated the long-term effect (12 days) of high calcium concentration (8 mM Ca2+) on C. raciborskii (T3 strain) growth, morphology, toxin content, and metabolism. Changes in protein expression profiles were investigated by proteomic analysis using 2D gel electrophoresis and mass spectrometry. A continued exposure to calcium had a pronounced effect on C. raciborskii (T3): it limited growth, decreased thricome length, increased chlorophyll-a content, altered toxin profile (although did not affect PST content, saxitoxin + neosaxitoxin), and inhibited the expression of proteins related to primary metabolism.
publishDate 2011
dc.date.none.fl_str_mv 2011-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400042
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400042
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822011000400042
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.42 n.4 2011
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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