The inhibitory effect of calcium on Cylindrospermopsis raciborskii (cyanobacteria) metabolism
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400042 |
Resumo: | Cylindrospermopsis raciborskii (Woloszynska) Seenaya & Subba Raju is a freshwater cyanobacterium of worldwide distribution. In the North-eastern region of Brazil many eutrophic water reservoirs are characterized by the dominance of C. raciborskii, with recurrent occurrence of blooms. These water bodies have high conductivity due to a high ionic concentration, and are defined as hard (with high values of CaCO3). In this study, we investigated the long-term effect (12 days) of high calcium concentration (8 mM Ca2+) on C. raciborskii (T3 strain) growth, morphology, toxin content, and metabolism. Changes in protein expression profiles were investigated by proteomic analysis using 2D gel electrophoresis and mass spectrometry. A continued exposure to calcium had a pronounced effect on C. raciborskii (T3): it limited growth, decreased thricome length, increased chlorophyll-a content, altered toxin profile (although did not affect PST content, saxitoxin + neosaxitoxin), and inhibited the expression of proteins related to primary metabolism. |
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Brazilian Journal of Microbiology |
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The inhibitory effect of calcium on Cylindrospermopsis raciborskii (cyanobacteria) metabolismcalciumCylindrospermopsisparalitic shellfish toxinproteomeCylindrospermopsis raciborskii (Woloszynska) Seenaya & Subba Raju is a freshwater cyanobacterium of worldwide distribution. In the North-eastern region of Brazil many eutrophic water reservoirs are characterized by the dominance of C. raciborskii, with recurrent occurrence of blooms. These water bodies have high conductivity due to a high ionic concentration, and are defined as hard (with high values of CaCO3). In this study, we investigated the long-term effect (12 days) of high calcium concentration (8 mM Ca2+) on C. raciborskii (T3 strain) growth, morphology, toxin content, and metabolism. Changes in protein expression profiles were investigated by proteomic analysis using 2D gel electrophoresis and mass spectrometry. A continued exposure to calcium had a pronounced effect on C. raciborskii (T3): it limited growth, decreased thricome length, increased chlorophyll-a content, altered toxin profile (although did not affect PST content, saxitoxin + neosaxitoxin), and inhibited the expression of proteins related to primary metabolism.Sociedade Brasileira de Microbiologia2011-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400042Brazilian Journal of Microbiology v.42 n.4 2011reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822011000400042info:eu-repo/semantics/openAccessCarneiro,Ronaldo LealAlípio,Ana Carla NascimentoBisch,Paulo MascarelloAzevedo,Sandra Maria Feliciano de Oliveira ePacheco,Ana Beatriz Furlanettoeng2015-06-30T00:00:00Zoai:scielo:S1517-83822011000400042Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2015-06-30T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
The inhibitory effect of calcium on Cylindrospermopsis raciborskii (cyanobacteria) metabolism |
title |
The inhibitory effect of calcium on Cylindrospermopsis raciborskii (cyanobacteria) metabolism |
spellingShingle |
The inhibitory effect of calcium on Cylindrospermopsis raciborskii (cyanobacteria) metabolism Carneiro,Ronaldo Leal calcium Cylindrospermopsis paralitic shellfish toxin proteome |
title_short |
The inhibitory effect of calcium on Cylindrospermopsis raciborskii (cyanobacteria) metabolism |
title_full |
The inhibitory effect of calcium on Cylindrospermopsis raciborskii (cyanobacteria) metabolism |
title_fullStr |
The inhibitory effect of calcium on Cylindrospermopsis raciborskii (cyanobacteria) metabolism |
title_full_unstemmed |
The inhibitory effect of calcium on Cylindrospermopsis raciborskii (cyanobacteria) metabolism |
title_sort |
The inhibitory effect of calcium on Cylindrospermopsis raciborskii (cyanobacteria) metabolism |
author |
Carneiro,Ronaldo Leal |
author_facet |
Carneiro,Ronaldo Leal Alípio,Ana Carla Nascimento Bisch,Paulo Mascarello Azevedo,Sandra Maria Feliciano de Oliveira e Pacheco,Ana Beatriz Furlanetto |
author_role |
author |
author2 |
Alípio,Ana Carla Nascimento Bisch,Paulo Mascarello Azevedo,Sandra Maria Feliciano de Oliveira e Pacheco,Ana Beatriz Furlanetto |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Carneiro,Ronaldo Leal Alípio,Ana Carla Nascimento Bisch,Paulo Mascarello Azevedo,Sandra Maria Feliciano de Oliveira e Pacheco,Ana Beatriz Furlanetto |
dc.subject.por.fl_str_mv |
calcium Cylindrospermopsis paralitic shellfish toxin proteome |
topic |
calcium Cylindrospermopsis paralitic shellfish toxin proteome |
description |
Cylindrospermopsis raciborskii (Woloszynska) Seenaya & Subba Raju is a freshwater cyanobacterium of worldwide distribution. In the North-eastern region of Brazil many eutrophic water reservoirs are characterized by the dominance of C. raciborskii, with recurrent occurrence of blooms. These water bodies have high conductivity due to a high ionic concentration, and are defined as hard (with high values of CaCO3). In this study, we investigated the long-term effect (12 days) of high calcium concentration (8 mM Ca2+) on C. raciborskii (T3 strain) growth, morphology, toxin content, and metabolism. Changes in protein expression profiles were investigated by proteomic analysis using 2D gel electrophoresis and mass spectrometry. A continued exposure to calcium had a pronounced effect on C. raciborskii (T3): it limited growth, decreased thricome length, increased chlorophyll-a content, altered toxin profile (although did not affect PST content, saxitoxin + neosaxitoxin), and inhibited the expression of proteins related to primary metabolism. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400042 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400042 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-83822011000400042 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.42 n.4 2011 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122204245458944 |