Heterogenic colonization patterns by Leptospira interrogans in Rattus norvegicus from urban slums

Detalhes bibliográficos
Autor(a) principal: Santos,Ana Amélia Nunes
Data de Publicação: 2015
Outros Autores: Figueira,Cláudio Pereira, Reis,Mitermayer Galvão dos, Costa,Federico, Ristow,Paula
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401161
Resumo: Abstract We evaluated the renal colonization by Leptospira interrogans in Rattus norvegicus (rats), as it is the major natural reservoir of urban leptospirosis. We caught 72 R. norvegicus, out of which 32 were found to be positive for L. interrogans by immunofluorescence assay. From these rats, we selected 17 and divided them into six groups based on the mass-age/sex. We performed the immunohistochemistry test against L. interrogans in the kidney sections of the rats and systematically counted the colonized tubules (CTs) in 20 fields. The proportion of positive fields varied from 5% to 95%. The number of CTs in 20 fields varied from 0.5 to 85.5. These differences were not related to age or sex of the animals. The characterization of leptospiral colonization patterns in the natural reservoirs is important to better understand the host-pathogen interactions in leptospirosis.
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spelling Heterogenic colonization patterns by Leptospira interrogans in Rattus norvegicus from urban slumsLeptospira interrogansrenal colonizationnatural reservoirsRattus norvegicusAbstract We evaluated the renal colonization by Leptospira interrogans in Rattus norvegicus (rats), as it is the major natural reservoir of urban leptospirosis. We caught 72 R. norvegicus, out of which 32 were found to be positive for L. interrogans by immunofluorescence assay. From these rats, we selected 17 and divided them into six groups based on the mass-age/sex. We performed the immunohistochemistry test against L. interrogans in the kidney sections of the rats and systematically counted the colonized tubules (CTs) in 20 fields. The proportion of positive fields varied from 5% to 95%. The number of CTs in 20 fields varied from 0.5 to 85.5. These differences were not related to age or sex of the animals. The characterization of leptospiral colonization patterns in the natural reservoirs is important to better understand the host-pathogen interactions in leptospirosis.Sociedade Brasileira de Microbiologia2015-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401161Brazilian Journal of Microbiology v.46 n.4 2015reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-838246420140873info:eu-repo/semantics/openAccessSantos,Ana Amélia NunesFigueira,Cláudio PereiraReis,Mitermayer Galvão dosCosta,FedericoRistow,Paulaeng2015-12-17T00:00:00Zoai:scielo:S1517-83822015000401161Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2015-12-17T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Heterogenic colonization patterns by Leptospira interrogans in Rattus norvegicus from urban slums
title Heterogenic colonization patterns by Leptospira interrogans in Rattus norvegicus from urban slums
spellingShingle Heterogenic colonization patterns by Leptospira interrogans in Rattus norvegicus from urban slums
Santos,Ana Amélia Nunes
Leptospira interrogans
renal colonization
natural reservoirs
Rattus norvegicus
title_short Heterogenic colonization patterns by Leptospira interrogans in Rattus norvegicus from urban slums
title_full Heterogenic colonization patterns by Leptospira interrogans in Rattus norvegicus from urban slums
title_fullStr Heterogenic colonization patterns by Leptospira interrogans in Rattus norvegicus from urban slums
title_full_unstemmed Heterogenic colonization patterns by Leptospira interrogans in Rattus norvegicus from urban slums
title_sort Heterogenic colonization patterns by Leptospira interrogans in Rattus norvegicus from urban slums
author Santos,Ana Amélia Nunes
author_facet Santos,Ana Amélia Nunes
Figueira,Cláudio Pereira
Reis,Mitermayer Galvão dos
Costa,Federico
Ristow,Paula
author_role author
author2 Figueira,Cláudio Pereira
Reis,Mitermayer Galvão dos
Costa,Federico
Ristow,Paula
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Santos,Ana Amélia Nunes
Figueira,Cláudio Pereira
Reis,Mitermayer Galvão dos
Costa,Federico
Ristow,Paula
dc.subject.por.fl_str_mv Leptospira interrogans
renal colonization
natural reservoirs
Rattus norvegicus
topic Leptospira interrogans
renal colonization
natural reservoirs
Rattus norvegicus
description Abstract We evaluated the renal colonization by Leptospira interrogans in Rattus norvegicus (rats), as it is the major natural reservoir of urban leptospirosis. We caught 72 R. norvegicus, out of which 32 were found to be positive for L. interrogans by immunofluorescence assay. From these rats, we selected 17 and divided them into six groups based on the mass-age/sex. We performed the immunohistochemistry test against L. interrogans in the kidney sections of the rats and systematically counted the colonized tubules (CTs) in 20 fields. The proportion of positive fields varied from 5% to 95%. The number of CTs in 20 fields varied from 0.5 to 85.5. These differences were not related to age or sex of the animals. The characterization of leptospiral colonization patterns in the natural reservoirs is important to better understand the host-pathogen interactions in leptospirosis.
publishDate 2015
dc.date.none.fl_str_mv 2015-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401161
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401161
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-838246420140873
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.46 n.4 2015
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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