Effects of oxygen transfer coefficient on dihydroxyacetone production from crude glycerol

Detalhes bibliográficos
Autor(a) principal: Zheng,Xiao-juan
Data de Publicação: 2016
Outros Autores: Jin,Kui-qi, Zhang,Lei, Wang,Gang, Liu,Yu-Peng
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100129
Resumo: Abstract The principal objective of this study was to evaluate the kinetics of dihydroxyacetone production by Gluconobacter frateurii CGMCC 5397 under different oxygen volumetric mass transfer coefficient (kLa) conditions in submerged bioreactors using biodiesel-derived crude glycerol as the carbon source. kLa is a key fermentation parameter for the production of dihydroxyacetone. Cultivations were conducted in baffled- and unbaffled-flask cultures (the kLa values were 24.32 h−1 and 52.05 h−1, respectively) and fed-batch cultures (the kLa values were held at 18.21 h−1, 46.03 h−1, and 82.14 h−1) to achieve high dihydroxyacetone concentration and productivity. The results showed that a high kLa could dramatically increase dihydroxyacetone concentrations and productivities. The baffled-flask culture (with a kLa of 52.05 h−1) favored glycerol utilization and dihydroxyacetone production, and a dihydroxyacetone concentration as high as 131.16 g/L was achieved. When the kLa was set to 82.14 h−1 in the fed-batch culture, the dihydroxyacetone concentration, productivity and yield were 175.44 g/L, 7.96 g/L/h and 0.89 g/g, respectively, all of which were significantly higher than those in previous studies and will benefit dihydroxyacetone industrial production.
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spelling Effects of oxygen transfer coefficient on dihydroxyacetone production from crude glycerolDihydroxyacetonekLaBiodiesel-derivedCrude glycerolGluconobacter frateuriiAbstract The principal objective of this study was to evaluate the kinetics of dihydroxyacetone production by Gluconobacter frateurii CGMCC 5397 under different oxygen volumetric mass transfer coefficient (kLa) conditions in submerged bioreactors using biodiesel-derived crude glycerol as the carbon source. kLa is a key fermentation parameter for the production of dihydroxyacetone. Cultivations were conducted in baffled- and unbaffled-flask cultures (the kLa values were 24.32 h−1 and 52.05 h−1, respectively) and fed-batch cultures (the kLa values were held at 18.21 h−1, 46.03 h−1, and 82.14 h−1) to achieve high dihydroxyacetone concentration and productivity. The results showed that a high kLa could dramatically increase dihydroxyacetone concentrations and productivities. The baffled-flask culture (with a kLa of 52.05 h−1) favored glycerol utilization and dihydroxyacetone production, and a dihydroxyacetone concentration as high as 131.16 g/L was achieved. When the kLa was set to 82.14 h−1 in the fed-batch culture, the dihydroxyacetone concentration, productivity and yield were 175.44 g/L, 7.96 g/L/h and 0.89 g/g, respectively, all of which were significantly higher than those in previous studies and will benefit dihydroxyacetone industrial production.Sociedade Brasileira de Microbiologia2016-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100129Brazilian Journal of Microbiology v.47 n.1 2016reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1016/j.bjm.2015.11.020info:eu-repo/semantics/openAccessZheng,Xiao-juanJin,Kui-qiZhang,LeiWang,GangLiu,Yu-Pengeng2016-03-01T00:00:00Zoai:scielo:S1517-83822016000100129Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2016-03-01T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Effects of oxygen transfer coefficient on dihydroxyacetone production from crude glycerol
title Effects of oxygen transfer coefficient on dihydroxyacetone production from crude glycerol
spellingShingle Effects of oxygen transfer coefficient on dihydroxyacetone production from crude glycerol
Zheng,Xiao-juan
Dihydroxyacetone
kLa
Biodiesel-derived
Crude glycerol
Gluconobacter frateurii
title_short Effects of oxygen transfer coefficient on dihydroxyacetone production from crude glycerol
title_full Effects of oxygen transfer coefficient on dihydroxyacetone production from crude glycerol
title_fullStr Effects of oxygen transfer coefficient on dihydroxyacetone production from crude glycerol
title_full_unstemmed Effects of oxygen transfer coefficient on dihydroxyacetone production from crude glycerol
title_sort Effects of oxygen transfer coefficient on dihydroxyacetone production from crude glycerol
author Zheng,Xiao-juan
author_facet Zheng,Xiao-juan
Jin,Kui-qi
Zhang,Lei
Wang,Gang
Liu,Yu-Peng
author_role author
author2 Jin,Kui-qi
Zhang,Lei
Wang,Gang
Liu,Yu-Peng
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Zheng,Xiao-juan
Jin,Kui-qi
Zhang,Lei
Wang,Gang
Liu,Yu-Peng
dc.subject.por.fl_str_mv Dihydroxyacetone
kLa
Biodiesel-derived
Crude glycerol
Gluconobacter frateurii
topic Dihydroxyacetone
kLa
Biodiesel-derived
Crude glycerol
Gluconobacter frateurii
description Abstract The principal objective of this study was to evaluate the kinetics of dihydroxyacetone production by Gluconobacter frateurii CGMCC 5397 under different oxygen volumetric mass transfer coefficient (kLa) conditions in submerged bioreactors using biodiesel-derived crude glycerol as the carbon source. kLa is a key fermentation parameter for the production of dihydroxyacetone. Cultivations were conducted in baffled- and unbaffled-flask cultures (the kLa values were 24.32 h−1 and 52.05 h−1, respectively) and fed-batch cultures (the kLa values were held at 18.21 h−1, 46.03 h−1, and 82.14 h−1) to achieve high dihydroxyacetone concentration and productivity. The results showed that a high kLa could dramatically increase dihydroxyacetone concentrations and productivities. The baffled-flask culture (with a kLa of 52.05 h−1) favored glycerol utilization and dihydroxyacetone production, and a dihydroxyacetone concentration as high as 131.16 g/L was achieved. When the kLa was set to 82.14 h−1 in the fed-batch culture, the dihydroxyacetone concentration, productivity and yield were 175.44 g/L, 7.96 g/L/h and 0.89 g/g, respectively, all of which were significantly higher than those in previous studies and will benefit dihydroxyacetone industrial production.
publishDate 2016
dc.date.none.fl_str_mv 2016-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100129
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100129
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1016/j.bjm.2015.11.020
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.47 n.1 2016
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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