A cytochemical study of acid carbohydrates on the surface of Candida lipolytica grown in tween 80-containing medium

Detalhes bibliográficos
Autor(a) principal: Nascimento,Aline E.
Data de Publicação: 2000
Outros Autores: Shariá,Allana E. do Nascimento, Lima,Marco Antonio Barbosa de, Campos-Takaki,Galba Maria
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000100008
Resumo: Carbohydrate-containing molecules were located on the surface of Candida lipolytica by using ruthenium red in a cytochemical study. The yeast was grown in media containing Tween 80. The surfactant, at 1.0% and 0.5%, was added to the culture medium in different intervals of time, correspondent to the beginning of exponential growth phase, mid of logarithimic phase and beginning of stationary growth phase. Control cultures were grown in a medium containing glucose. The growth of the yeast in media containing glucose and Tween 80 induced changes in the pattern of distribution and location of acid polyssacharides in the cell wall of the microorganism. In adition, the pattern also changed according to Tween 80 concentration. The influence of Tween 80 on cellular carbohydrate expression is discussed.
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spelling A cytochemical study of acid carbohydrates on the surface of Candida lipolytica grown in tween 80-containing mediumCandida lipolyticacarbohydrate cytochemistryfungi ultrastructureCarbohydrate-containing molecules were located on the surface of Candida lipolytica by using ruthenium red in a cytochemical study. The yeast was grown in media containing Tween 80. The surfactant, at 1.0% and 0.5%, was added to the culture medium in different intervals of time, correspondent to the beginning of exponential growth phase, mid of logarithimic phase and beginning of stationary growth phase. Control cultures were grown in a medium containing glucose. The growth of the yeast in media containing glucose and Tween 80 induced changes in the pattern of distribution and location of acid polyssacharides in the cell wall of the microorganism. In adition, the pattern also changed according to Tween 80 concentration. The influence of Tween 80 on cellular carbohydrate expression is discussed.Sociedade Brasileira de Microbiologia2000-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000100008Brazilian Journal of Microbiology v.31 n.1 2000reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822000000100008info:eu-repo/semantics/openAccessNascimento,Aline E.Shariá,Allana E. do NascimentoLima,Marco Antonio Barbosa deCampos-Takaki,Galba Mariaeng2000-08-25T00:00:00Zoai:scielo:S1517-83822000000100008Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2000-08-25T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv A cytochemical study of acid carbohydrates on the surface of Candida lipolytica grown in tween 80-containing medium
title A cytochemical study of acid carbohydrates on the surface of Candida lipolytica grown in tween 80-containing medium
spellingShingle A cytochemical study of acid carbohydrates on the surface of Candida lipolytica grown in tween 80-containing medium
Nascimento,Aline E.
Candida lipolytica
carbohydrate cytochemistry
fungi ultrastructure
title_short A cytochemical study of acid carbohydrates on the surface of Candida lipolytica grown in tween 80-containing medium
title_full A cytochemical study of acid carbohydrates on the surface of Candida lipolytica grown in tween 80-containing medium
title_fullStr A cytochemical study of acid carbohydrates on the surface of Candida lipolytica grown in tween 80-containing medium
title_full_unstemmed A cytochemical study of acid carbohydrates on the surface of Candida lipolytica grown in tween 80-containing medium
title_sort A cytochemical study of acid carbohydrates on the surface of Candida lipolytica grown in tween 80-containing medium
author Nascimento,Aline E.
author_facet Nascimento,Aline E.
Shariá,Allana E. do Nascimento
Lima,Marco Antonio Barbosa de
Campos-Takaki,Galba Maria
author_role author
author2 Shariá,Allana E. do Nascimento
Lima,Marco Antonio Barbosa de
Campos-Takaki,Galba Maria
author2_role author
author
author
dc.contributor.author.fl_str_mv Nascimento,Aline E.
Shariá,Allana E. do Nascimento
Lima,Marco Antonio Barbosa de
Campos-Takaki,Galba Maria
dc.subject.por.fl_str_mv Candida lipolytica
carbohydrate cytochemistry
fungi ultrastructure
topic Candida lipolytica
carbohydrate cytochemistry
fungi ultrastructure
description Carbohydrate-containing molecules were located on the surface of Candida lipolytica by using ruthenium red in a cytochemical study. The yeast was grown in media containing Tween 80. The surfactant, at 1.0% and 0.5%, was added to the culture medium in different intervals of time, correspondent to the beginning of exponential growth phase, mid of logarithimic phase and beginning of stationary growth phase. Control cultures were grown in a medium containing glucose. The growth of the yeast in media containing glucose and Tween 80 induced changes in the pattern of distribution and location of acid polyssacharides in the cell wall of the microorganism. In adition, the pattern also changed according to Tween 80 concentration. The influence of Tween 80 on cellular carbohydrate expression is discussed.
publishDate 2000
dc.date.none.fl_str_mv 2000-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000100008
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000100008
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822000000100008
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.31 n.1 2000
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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