Use of remazol blue dyed avicel for the determination of cellulolytic activity in basidiomycetes

Detalhes bibliográficos
Autor(a) principal: Correia,Marcos José
Data de Publicação: 1998
Outros Autores: Pereira Junior,José Antônio de Sousa, Santos,Jefferson Cunha dos, Cavalcanti,Maria Auxiliadora de Queiroz
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista de Microbiologia
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141998000400010
Resumo: A modified method for direct determination of cellulolytic activity using Avicel colored with Remazol Brilliant Blue R (RBBR) in Agar test tubes is discussed. Refinements were introduced in a simple method for quantitation of cellulase activity, based on the release of dye from Avicel-RBBR medium by the enzymatic hydrolysis. Modifications in Avicel-dye preparation were enhanced and a spectrophotometer for direct OD measurement in agar test tubes used. The use of a spectrophotometer improved the precision of the collected data, since absorbance measurements could be done at the maximum wavelenght for RBBR (595 nm).
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spelling Use of remazol blue dyed avicel for the determination of cellulolytic activity in basidiomycetesAvicelcellulaseRemazol BlueLentinus edodesPleurotus ostreatusA modified method for direct determination of cellulolytic activity using Avicel colored with Remazol Brilliant Blue R (RBBR) in Agar test tubes is discussed. Refinements were introduced in a simple method for quantitation of cellulase activity, based on the release of dye from Avicel-RBBR medium by the enzymatic hydrolysis. Modifications in Avicel-dye preparation were enhanced and a spectrophotometer for direct OD measurement in agar test tubes used. The use of a spectrophotometer improved the precision of the collected data, since absorbance measurements could be done at the maximum wavelenght for RBBR (595 nm).Sociedade Brasileira de Microbiologia1998-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141998000400010Revista de Microbiologia v.29 n.4 1998reponame:Revista de Microbiologiainstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S0001-37141998000400010info:eu-repo/semantics/openAccessCorreia,Marcos JoséPereira Junior,José Antônio de SousaSantos,Jefferson Cunha dosCavalcanti,Maria Auxiliadora de Queirozeng1999-05-27T00:00:00Zoai:scielo:S0001-37141998000400010Revistahttps://www.scielo.br/j/rm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||revmicro@icb.usp.br0001-37140001-3714opendoar:1999-05-27T00:00Revista de Microbiologia - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Use of remazol blue dyed avicel for the determination of cellulolytic activity in basidiomycetes
title Use of remazol blue dyed avicel for the determination of cellulolytic activity in basidiomycetes
spellingShingle Use of remazol blue dyed avicel for the determination of cellulolytic activity in basidiomycetes
Correia,Marcos José
Avicel
cellulase
Remazol Blue
Lentinus edodes
Pleurotus ostreatus
title_short Use of remazol blue dyed avicel for the determination of cellulolytic activity in basidiomycetes
title_full Use of remazol blue dyed avicel for the determination of cellulolytic activity in basidiomycetes
title_fullStr Use of remazol blue dyed avicel for the determination of cellulolytic activity in basidiomycetes
title_full_unstemmed Use of remazol blue dyed avicel for the determination of cellulolytic activity in basidiomycetes
title_sort Use of remazol blue dyed avicel for the determination of cellulolytic activity in basidiomycetes
author Correia,Marcos José
author_facet Correia,Marcos José
Pereira Junior,José Antônio de Sousa
Santos,Jefferson Cunha dos
Cavalcanti,Maria Auxiliadora de Queiroz
author_role author
author2 Pereira Junior,José Antônio de Sousa
Santos,Jefferson Cunha dos
Cavalcanti,Maria Auxiliadora de Queiroz
author2_role author
author
author
dc.contributor.author.fl_str_mv Correia,Marcos José
Pereira Junior,José Antônio de Sousa
Santos,Jefferson Cunha dos
Cavalcanti,Maria Auxiliadora de Queiroz
dc.subject.por.fl_str_mv Avicel
cellulase
Remazol Blue
Lentinus edodes
Pleurotus ostreatus
topic Avicel
cellulase
Remazol Blue
Lentinus edodes
Pleurotus ostreatus
description A modified method for direct determination of cellulolytic activity using Avicel colored with Remazol Brilliant Blue R (RBBR) in Agar test tubes is discussed. Refinements were introduced in a simple method for quantitation of cellulase activity, based on the release of dye from Avicel-RBBR medium by the enzymatic hydrolysis. Modifications in Avicel-dye preparation were enhanced and a spectrophotometer for direct OD measurement in agar test tubes used. The use of a spectrophotometer improved the precision of the collected data, since absorbance measurements could be done at the maximum wavelenght for RBBR (595 nm).
publishDate 1998
dc.date.none.fl_str_mv 1998-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141998000400010
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141998000400010
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0001-37141998000400010
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Revista de Microbiologia v.29 n.4 1998
reponame:Revista de Microbiologia
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Revista de Microbiologia
collection Revista de Microbiologia
repository.name.fl_str_mv Revista de Microbiologia - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||revmicro@icb.usp.br
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