Production of amylases by Aspergillus tamarii
Autor(a) principal: | |
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Data de Publicação: | 1999 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista de Microbiologia |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000200014 |
Resumo: | A strain of Aspergillus tamarii, a filamentous fungus isolated from soil, was able to produce both <FONT FACE="Symbol">a</FONT>-amylase and glucoamylase activities in mineral media supplemented with 1% (w/v) starch or maltose as the carbon source. Static cultivation led to significantly higher yields than those obtained using shaking culture. The production of amylases was tolerant to a wide range of initial culture pH values (from 4 to 10) and temperature (from 25 to 42oC). Two amylases, one <FONT FACE="Symbol">a</FONT>-amylase and one glucoamylase, were separated by ion exchange chromatography. Both partially purified enzymes had optimal activities at pH values between 4.5 and 6.0 and were stable under acid conditions (pH 4.0-7.0). The enzymes exhibited optimal activities at temperatures between 50o and 60o C and were stable for more than ten hours at 55oC. |
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Revista de Microbiologia |
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Production of amylases by Aspergillus tamarii<FONT FACE=Symbol>a</FONT>-amylaseAspergillus tamariiglucoamylaseA strain of Aspergillus tamarii, a filamentous fungus isolated from soil, was able to produce both <FONT FACE="Symbol">a</FONT>-amylase and glucoamylase activities in mineral media supplemented with 1% (w/v) starch or maltose as the carbon source. Static cultivation led to significantly higher yields than those obtained using shaking culture. The production of amylases was tolerant to a wide range of initial culture pH values (from 4 to 10) and temperature (from 25 to 42oC). Two amylases, one <FONT FACE="Symbol">a</FONT>-amylase and one glucoamylase, were separated by ion exchange chromatography. Both partially purified enzymes had optimal activities at pH values between 4.5 and 6.0 and were stable under acid conditions (pH 4.0-7.0). The enzymes exhibited optimal activities at temperatures between 50o and 60o C and were stable for more than ten hours at 55oC.Sociedade Brasileira de Microbiologia1999-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000200014Revista de Microbiologia v.30 n.2 1999reponame:Revista de Microbiologiainstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S0001-37141999000200014info:eu-repo/semantics/openAccessMoreira,Fabiana GuillenLima,Francieli Arrias dePedrinho,Sophia Renata FazzanoLenartovicz,VeridianaSouza,Cristina Giatti Marques dePeralta,Rosane Marinaeng2000-01-06T00:00:00Zoai:scielo:S0001-37141999000200014Revistahttps://www.scielo.br/j/rm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||revmicro@icb.usp.br0001-37140001-3714opendoar:2000-01-06T00:00Revista de Microbiologia - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Production of amylases by Aspergillus tamarii |
title |
Production of amylases by Aspergillus tamarii |
spellingShingle |
Production of amylases by Aspergillus tamarii Moreira,Fabiana Guillen <FONT FACE=Symbol>a</FONT>-amylase Aspergillus tamarii glucoamylase |
title_short |
Production of amylases by Aspergillus tamarii |
title_full |
Production of amylases by Aspergillus tamarii |
title_fullStr |
Production of amylases by Aspergillus tamarii |
title_full_unstemmed |
Production of amylases by Aspergillus tamarii |
title_sort |
Production of amylases by Aspergillus tamarii |
author |
Moreira,Fabiana Guillen |
author_facet |
Moreira,Fabiana Guillen Lima,Francieli Arrias de Pedrinho,Sophia Renata Fazzano Lenartovicz,Veridiana Souza,Cristina Giatti Marques de Peralta,Rosane Marina |
author_role |
author |
author2 |
Lima,Francieli Arrias de Pedrinho,Sophia Renata Fazzano Lenartovicz,Veridiana Souza,Cristina Giatti Marques de Peralta,Rosane Marina |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Moreira,Fabiana Guillen Lima,Francieli Arrias de Pedrinho,Sophia Renata Fazzano Lenartovicz,Veridiana Souza,Cristina Giatti Marques de Peralta,Rosane Marina |
dc.subject.por.fl_str_mv |
<FONT FACE=Symbol>a</FONT>-amylase Aspergillus tamarii glucoamylase |
topic |
<FONT FACE=Symbol>a</FONT>-amylase Aspergillus tamarii glucoamylase |
description |
A strain of Aspergillus tamarii, a filamentous fungus isolated from soil, was able to produce both <FONT FACE="Symbol">a</FONT>-amylase and glucoamylase activities in mineral media supplemented with 1% (w/v) starch or maltose as the carbon source. Static cultivation led to significantly higher yields than those obtained using shaking culture. The production of amylases was tolerant to a wide range of initial culture pH values (from 4 to 10) and temperature (from 25 to 42oC). Two amylases, one <FONT FACE="Symbol">a</FONT>-amylase and one glucoamylase, were separated by ion exchange chromatography. Both partially purified enzymes had optimal activities at pH values between 4.5 and 6.0 and were stable under acid conditions (pH 4.0-7.0). The enzymes exhibited optimal activities at temperatures between 50o and 60o C and were stable for more than ten hours at 55oC. |
publishDate |
1999 |
dc.date.none.fl_str_mv |
1999-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000200014 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000200014 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0001-37141999000200014 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Revista de Microbiologia v.30 n.2 1999 reponame:Revista de Microbiologia instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Revista de Microbiologia |
collection |
Revista de Microbiologia |
repository.name.fl_str_mv |
Revista de Microbiologia - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||revmicro@icb.usp.br |
_version_ |
1754821030194446336 |