CARNOSINE AVOIDS THE OXIDATIVE DAMAGE CAUSED BY INTENSE EXERCISE ON RAT SOLEUS MUSCLE
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista brasileira de medicina do esporte (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-86922020000100011 |
Resumo: | ABSTRACT Introduction: Intense physical exercise affects the balance between the production of reactive oxygen species and antioxidant defense in the muscle. Carnosine is a cytoplasmic dipeptide composed of the amino acids β-alanine and histidine. Objective: This study aimed to evaluate the effect of carnosine and its precursor β-alanine on oxidative damage caused by intense physical exercise in the soleus muscle of rats. Methods: Male Wistar rats weighing between 200 and 240 g were divided into four groups: control, exercise, exercise + β-alanine and exercise + carnosine. The animals from the groups that underwent the exercise ran on a treadmill for 60 minutes at 25 m/minute. Factors related to muscle damage and oxidative stress were assessed in soleus muscle homogenate and blood serum. Results: The exercise promoted muscle damage, as observed through increased serum activity of enzymes aspartate aminotransferase and creatine kinase. It also induced oxidative stress in soleus muscle, as seen by the increased activity of the enzymes glutathione peroxidase and glutathione reductase, decreased concentration of reduced glutathione, and increased concentration of malondialdehyde, an indicator of lipid peroxidation. Carnosine kept the creatine kinase, glutathione peroxidase and glutathione reductase enzyme activity values, and the concentration of reduced glutathione and malondialdehyde, close to those of the control group. Conclusion: The results indicate that pretreatment with carnosine protected the rat soleus muscle against oxidative damage and consequent injury caused by intense physical exercise. Level of evidence II; Therapeutic studies-Investigating the treatment results. |
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CARNOSINE AVOIDS THE OXIDATIVE DAMAGE CAUSED BY INTENSE EXERCISE ON RAT SOLEUS MUSCLEAntioxidantsOxidative stressFree radicalsExercise testABSTRACT Introduction: Intense physical exercise affects the balance between the production of reactive oxygen species and antioxidant defense in the muscle. Carnosine is a cytoplasmic dipeptide composed of the amino acids β-alanine and histidine. Objective: This study aimed to evaluate the effect of carnosine and its precursor β-alanine on oxidative damage caused by intense physical exercise in the soleus muscle of rats. Methods: Male Wistar rats weighing between 200 and 240 g were divided into four groups: control, exercise, exercise + β-alanine and exercise + carnosine. The animals from the groups that underwent the exercise ran on a treadmill for 60 minutes at 25 m/minute. Factors related to muscle damage and oxidative stress were assessed in soleus muscle homogenate and blood serum. Results: The exercise promoted muscle damage, as observed through increased serum activity of enzymes aspartate aminotransferase and creatine kinase. It also induced oxidative stress in soleus muscle, as seen by the increased activity of the enzymes glutathione peroxidase and glutathione reductase, decreased concentration of reduced glutathione, and increased concentration of malondialdehyde, an indicator of lipid peroxidation. Carnosine kept the creatine kinase, glutathione peroxidase and glutathione reductase enzyme activity values, and the concentration of reduced glutathione and malondialdehyde, close to those of the control group. Conclusion: The results indicate that pretreatment with carnosine protected the rat soleus muscle against oxidative damage and consequent injury caused by intense physical exercise. Level of evidence II; Therapeutic studies-Investigating the treatment results.Sociedade Brasileira de Medicina do Exercício e do Esporte2020-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-86922020000100011Revista Brasileira de Medicina do Esporte v.26 n.1 2020reponame:Revista brasileira de medicina do esporte (Online)instname:Sociedade Brasileira de Medicina do Exercício e do Esporte (SBMEE)instacron:SBMEE10.1590/1517-869220202601158444info:eu-repo/semantics/openAccessBortolatto,Guilherme PedriniMedeiros,Hyllana Catarine Dias deGuelfi,MarieliTavares,Marco AurélioMazzo,MeirieleMingatto,Fábio Erminioeng2020-01-09T00:00:00Zoai:scielo:S1517-86922020000100011Revistahttp://www.scielo.br/rbmeONGhttps://old.scielo.br/oai/scielo-oai.php||revista@medicinadoesporte.org.br1806-99401517-8692opendoar:2020-01-09T00:00Revista brasileira de medicina do esporte (Online) - Sociedade Brasileira de Medicina do Exercício e do Esporte (SBMEE)false |
dc.title.none.fl_str_mv |
CARNOSINE AVOIDS THE OXIDATIVE DAMAGE CAUSED BY INTENSE EXERCISE ON RAT SOLEUS MUSCLE |
title |
CARNOSINE AVOIDS THE OXIDATIVE DAMAGE CAUSED BY INTENSE EXERCISE ON RAT SOLEUS MUSCLE |
spellingShingle |
CARNOSINE AVOIDS THE OXIDATIVE DAMAGE CAUSED BY INTENSE EXERCISE ON RAT SOLEUS MUSCLE Bortolatto,Guilherme Pedrini Antioxidants Oxidative stress Free radicals Exercise test |
title_short |
CARNOSINE AVOIDS THE OXIDATIVE DAMAGE CAUSED BY INTENSE EXERCISE ON RAT SOLEUS MUSCLE |
title_full |
CARNOSINE AVOIDS THE OXIDATIVE DAMAGE CAUSED BY INTENSE EXERCISE ON RAT SOLEUS MUSCLE |
title_fullStr |
CARNOSINE AVOIDS THE OXIDATIVE DAMAGE CAUSED BY INTENSE EXERCISE ON RAT SOLEUS MUSCLE |
title_full_unstemmed |
CARNOSINE AVOIDS THE OXIDATIVE DAMAGE CAUSED BY INTENSE EXERCISE ON RAT SOLEUS MUSCLE |
title_sort |
CARNOSINE AVOIDS THE OXIDATIVE DAMAGE CAUSED BY INTENSE EXERCISE ON RAT SOLEUS MUSCLE |
author |
Bortolatto,Guilherme Pedrini |
author_facet |
Bortolatto,Guilherme Pedrini Medeiros,Hyllana Catarine Dias de Guelfi,Marieli Tavares,Marco Aurélio Mazzo,Meiriele Mingatto,Fábio Erminio |
author_role |
author |
author2 |
Medeiros,Hyllana Catarine Dias de Guelfi,Marieli Tavares,Marco Aurélio Mazzo,Meiriele Mingatto,Fábio Erminio |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Bortolatto,Guilherme Pedrini Medeiros,Hyllana Catarine Dias de Guelfi,Marieli Tavares,Marco Aurélio Mazzo,Meiriele Mingatto,Fábio Erminio |
dc.subject.por.fl_str_mv |
Antioxidants Oxidative stress Free radicals Exercise test |
topic |
Antioxidants Oxidative stress Free radicals Exercise test |
description |
ABSTRACT Introduction: Intense physical exercise affects the balance between the production of reactive oxygen species and antioxidant defense in the muscle. Carnosine is a cytoplasmic dipeptide composed of the amino acids β-alanine and histidine. Objective: This study aimed to evaluate the effect of carnosine and its precursor β-alanine on oxidative damage caused by intense physical exercise in the soleus muscle of rats. Methods: Male Wistar rats weighing between 200 and 240 g were divided into four groups: control, exercise, exercise + β-alanine and exercise + carnosine. The animals from the groups that underwent the exercise ran on a treadmill for 60 minutes at 25 m/minute. Factors related to muscle damage and oxidative stress were assessed in soleus muscle homogenate and blood serum. Results: The exercise promoted muscle damage, as observed through increased serum activity of enzymes aspartate aminotransferase and creatine kinase. It also induced oxidative stress in soleus muscle, as seen by the increased activity of the enzymes glutathione peroxidase and glutathione reductase, decreased concentration of reduced glutathione, and increased concentration of malondialdehyde, an indicator of lipid peroxidation. Carnosine kept the creatine kinase, glutathione peroxidase and glutathione reductase enzyme activity values, and the concentration of reduced glutathione and malondialdehyde, close to those of the control group. Conclusion: The results indicate that pretreatment with carnosine protected the rat soleus muscle against oxidative damage and consequent injury caused by intense physical exercise. Level of evidence II; Therapeutic studies-Investigating the treatment results. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-02-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-86922020000100011 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-86922020000100011 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1517-869220202601158444 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Medicina do Exercício e do Esporte |
publisher.none.fl_str_mv |
Sociedade Brasileira de Medicina do Exercício e do Esporte |
dc.source.none.fl_str_mv |
Revista Brasileira de Medicina do Esporte v.26 n.1 2020 reponame:Revista brasileira de medicina do esporte (Online) instname:Sociedade Brasileira de Medicina do Exercício e do Esporte (SBMEE) instacron:SBMEE |
instname_str |
Sociedade Brasileira de Medicina do Exercício e do Esporte (SBMEE) |
instacron_str |
SBMEE |
institution |
SBMEE |
reponame_str |
Revista brasileira de medicina do esporte (Online) |
collection |
Revista brasileira de medicina do esporte (Online) |
repository.name.fl_str_mv |
Revista brasileira de medicina do esporte (Online) - Sociedade Brasileira de Medicina do Exercício e do Esporte (SBMEE) |
repository.mail.fl_str_mv |
||revista@medicinadoesporte.org.br |
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1752122236828909568 |