CARNOSINE AVOIDS THE OXIDATIVE DAMAGE CAUSED BY INTENSE EXERCISE ON RAT SOLEUS MUSCLE

Detalhes bibliográficos
Autor(a) principal: Bortolatto,Guilherme Pedrini
Data de Publicação: 2020
Outros Autores: Medeiros,Hyllana Catarine Dias de, Guelfi,Marieli, Tavares,Marco Aurélio, Mazzo,Meiriele, Mingatto,Fábio Erminio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista brasileira de medicina do esporte (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-86922020000100011
Resumo: ABSTRACT Introduction: Intense physical exercise affects the balance between the production of reactive oxygen species and antioxidant defense in the muscle. Carnosine is a cytoplasmic dipeptide composed of the amino acids β-alanine and histidine. Objective: This study aimed to evaluate the effect of carnosine and its precursor β-alanine on oxidative damage caused by intense physical exercise in the soleus muscle of rats. Methods: Male Wistar rats weighing between 200 and 240 g were divided into four groups: control, exercise, exercise + β-alanine and exercise + carnosine. The animals from the groups that underwent the exercise ran on a treadmill for 60 minutes at 25 m/minute. Factors related to muscle damage and oxidative stress were assessed in soleus muscle homogenate and blood serum. Results: The exercise promoted muscle damage, as observed through increased serum activity of enzymes aspartate aminotransferase and creatine kinase. It also induced oxidative stress in soleus muscle, as seen by the increased activity of the enzymes glutathione peroxidase and glutathione reductase, decreased concentration of reduced glutathione, and increased concentration of malondialdehyde, an indicator of lipid peroxidation. Carnosine kept the creatine kinase, glutathione peroxidase and glutathione reductase enzyme activity values, and the concentration of reduced glutathione and malondialdehyde, close to those of the control group. Conclusion: The results indicate that pretreatment with carnosine protected the rat soleus muscle against oxidative damage and consequent injury caused by intense physical exercise. Level of evidence II; Therapeutic studies-Investigating the treatment results.
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spelling CARNOSINE AVOIDS THE OXIDATIVE DAMAGE CAUSED BY INTENSE EXERCISE ON RAT SOLEUS MUSCLEAntioxidantsOxidative stressFree radicalsExercise testABSTRACT Introduction: Intense physical exercise affects the balance between the production of reactive oxygen species and antioxidant defense in the muscle. Carnosine is a cytoplasmic dipeptide composed of the amino acids β-alanine and histidine. Objective: This study aimed to evaluate the effect of carnosine and its precursor β-alanine on oxidative damage caused by intense physical exercise in the soleus muscle of rats. Methods: Male Wistar rats weighing between 200 and 240 g were divided into four groups: control, exercise, exercise + β-alanine and exercise + carnosine. The animals from the groups that underwent the exercise ran on a treadmill for 60 minutes at 25 m/minute. Factors related to muscle damage and oxidative stress were assessed in soleus muscle homogenate and blood serum. Results: The exercise promoted muscle damage, as observed through increased serum activity of enzymes aspartate aminotransferase and creatine kinase. It also induced oxidative stress in soleus muscle, as seen by the increased activity of the enzymes glutathione peroxidase and glutathione reductase, decreased concentration of reduced glutathione, and increased concentration of malondialdehyde, an indicator of lipid peroxidation. Carnosine kept the creatine kinase, glutathione peroxidase and glutathione reductase enzyme activity values, and the concentration of reduced glutathione and malondialdehyde, close to those of the control group. Conclusion: The results indicate that pretreatment with carnosine protected the rat soleus muscle against oxidative damage and consequent injury caused by intense physical exercise. Level of evidence II; Therapeutic studies-Investigating the treatment results.Sociedade Brasileira de Medicina do Exercício e do Esporte2020-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-86922020000100011Revista Brasileira de Medicina do Esporte v.26 n.1 2020reponame:Revista brasileira de medicina do esporte (Online)instname:Sociedade Brasileira de Medicina do Exercício e do Esporte (SBMEE)instacron:SBMEE10.1590/1517-869220202601158444info:eu-repo/semantics/openAccessBortolatto,Guilherme PedriniMedeiros,Hyllana Catarine Dias deGuelfi,MarieliTavares,Marco AurélioMazzo,MeirieleMingatto,Fábio Erminioeng2020-01-09T00:00:00Zoai:scielo:S1517-86922020000100011Revistahttp://www.scielo.br/rbmeONGhttps://old.scielo.br/oai/scielo-oai.php||revista@medicinadoesporte.org.br1806-99401517-8692opendoar:2020-01-09T00:00Revista brasileira de medicina do esporte (Online) - Sociedade Brasileira de Medicina do Exercício e do Esporte (SBMEE)false
dc.title.none.fl_str_mv CARNOSINE AVOIDS THE OXIDATIVE DAMAGE CAUSED BY INTENSE EXERCISE ON RAT SOLEUS MUSCLE
title CARNOSINE AVOIDS THE OXIDATIVE DAMAGE CAUSED BY INTENSE EXERCISE ON RAT SOLEUS MUSCLE
spellingShingle CARNOSINE AVOIDS THE OXIDATIVE DAMAGE CAUSED BY INTENSE EXERCISE ON RAT SOLEUS MUSCLE
Bortolatto,Guilherme Pedrini
Antioxidants
Oxidative stress
Free radicals
Exercise test
title_short CARNOSINE AVOIDS THE OXIDATIVE DAMAGE CAUSED BY INTENSE EXERCISE ON RAT SOLEUS MUSCLE
title_full CARNOSINE AVOIDS THE OXIDATIVE DAMAGE CAUSED BY INTENSE EXERCISE ON RAT SOLEUS MUSCLE
title_fullStr CARNOSINE AVOIDS THE OXIDATIVE DAMAGE CAUSED BY INTENSE EXERCISE ON RAT SOLEUS MUSCLE
title_full_unstemmed CARNOSINE AVOIDS THE OXIDATIVE DAMAGE CAUSED BY INTENSE EXERCISE ON RAT SOLEUS MUSCLE
title_sort CARNOSINE AVOIDS THE OXIDATIVE DAMAGE CAUSED BY INTENSE EXERCISE ON RAT SOLEUS MUSCLE
author Bortolatto,Guilherme Pedrini
author_facet Bortolatto,Guilherme Pedrini
Medeiros,Hyllana Catarine Dias de
Guelfi,Marieli
Tavares,Marco Aurélio
Mazzo,Meiriele
Mingatto,Fábio Erminio
author_role author
author2 Medeiros,Hyllana Catarine Dias de
Guelfi,Marieli
Tavares,Marco Aurélio
Mazzo,Meiriele
Mingatto,Fábio Erminio
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Bortolatto,Guilherme Pedrini
Medeiros,Hyllana Catarine Dias de
Guelfi,Marieli
Tavares,Marco Aurélio
Mazzo,Meiriele
Mingatto,Fábio Erminio
dc.subject.por.fl_str_mv Antioxidants
Oxidative stress
Free radicals
Exercise test
topic Antioxidants
Oxidative stress
Free radicals
Exercise test
description ABSTRACT Introduction: Intense physical exercise affects the balance between the production of reactive oxygen species and antioxidant defense in the muscle. Carnosine is a cytoplasmic dipeptide composed of the amino acids β-alanine and histidine. Objective: This study aimed to evaluate the effect of carnosine and its precursor β-alanine on oxidative damage caused by intense physical exercise in the soleus muscle of rats. Methods: Male Wistar rats weighing between 200 and 240 g were divided into four groups: control, exercise, exercise + β-alanine and exercise + carnosine. The animals from the groups that underwent the exercise ran on a treadmill for 60 minutes at 25 m/minute. Factors related to muscle damage and oxidative stress were assessed in soleus muscle homogenate and blood serum. Results: The exercise promoted muscle damage, as observed through increased serum activity of enzymes aspartate aminotransferase and creatine kinase. It also induced oxidative stress in soleus muscle, as seen by the increased activity of the enzymes glutathione peroxidase and glutathione reductase, decreased concentration of reduced glutathione, and increased concentration of malondialdehyde, an indicator of lipid peroxidation. Carnosine kept the creatine kinase, glutathione peroxidase and glutathione reductase enzyme activity values, and the concentration of reduced glutathione and malondialdehyde, close to those of the control group. Conclusion: The results indicate that pretreatment with carnosine protected the rat soleus muscle against oxidative damage and consequent injury caused by intense physical exercise. Level of evidence II; Therapeutic studies-Investigating the treatment results.
publishDate 2020
dc.date.none.fl_str_mv 2020-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
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dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-86922020000100011
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dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1517-869220202601158444
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Medicina do Exercício e do Esporte
publisher.none.fl_str_mv Sociedade Brasileira de Medicina do Exercício e do Esporte
dc.source.none.fl_str_mv Revista Brasileira de Medicina do Esporte v.26 n.1 2020
reponame:Revista brasileira de medicina do esporte (Online)
instname:Sociedade Brasileira de Medicina do Exercício e do Esporte (SBMEE)
instacron:SBMEE
instname_str Sociedade Brasileira de Medicina do Exercício e do Esporte (SBMEE)
instacron_str SBMEE
institution SBMEE
reponame_str Revista brasileira de medicina do esporte (Online)
collection Revista brasileira de medicina do esporte (Online)
repository.name.fl_str_mv Revista brasileira de medicina do esporte (Online) - Sociedade Brasileira de Medicina do Exercício e do Esporte (SBMEE)
repository.mail.fl_str_mv ||revista@medicinadoesporte.org.br
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