EFFECTS OF EXHAUSTIVE EXERCISE AND CONTUSION ON AUTOPHAGY-RELATED FACTORS IN SKELETAL MUSCLE OF RATS
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista brasileira de medicina do esporte (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-86922021000600563 |
Resumo: | ABSTRACT Objective: To study the effects of exhaustive exercise and contusion on autophagy-related factors Beclin1, LC3 and PINK1 expression in the skeletal muscle of rats. Methods: Forty-two male SD rats were randomly divided into 7 groups, 6 rats in each group: C, D0, D24, D48, E0, E24, and E48. Each group of rats was killed and dissected at the different respective time points specified above. The whole quadriceps femoris of the left hind limbs were removed and divided into two parts, one for mRNAs of Beclin1, LC3 and PINK1 by real-time fluorescent quantitative PCR, and the other for LC3 protein by Western blotting. Results: Compared with group C, the contents of Beclin1 mRNA, PINK1 mRNA, and LC3 mRNA in the immediate exhaustive exercise group (E0) were significantly reduced p<0.01. However, the levels of PINK1 mRNA, LC3 mRNA, and LC3 protein in skeletal muscle cells increased significantly in the 48 hours after exhaustion (E48) p<0.05, suggesting that cell autophagy had an increasing trend during the recovery period. Meanwhile, compared with the C group, the contents of Beclin1 mRNA, PINK1 mRNA, and LC3 mRNA in the immediate blunt contusion group (D0) increased significantly p<0.01 and were followed by a downward trend. Conclusion: Generally, there were differences between the blunt contusion and exhausted exercise models at each recovery phase. The gene expression of the autophagy-related factors was not high in the early exhaustive exercise recovery phase and subsequently followed an upward trend. But the above factors increased significantly in the immediate and early recovery phases after blunt contusion. Injury from blunt contusion may be more severe than exhaustive exercise-induced-injury, so the autophagy starts earlier according to the changes in autophagy-related factors. Level of evidence III; Therapeutic studies investigating the results of treatment. |
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EFFECTS OF EXHAUSTIVE EXERCISE AND CONTUSION ON AUTOPHAGY-RELATED FACTORS IN SKELETAL MUSCLE OF RATSContusionBeclin1ExerciseABSTRACT Objective: To study the effects of exhaustive exercise and contusion on autophagy-related factors Beclin1, LC3 and PINK1 expression in the skeletal muscle of rats. Methods: Forty-two male SD rats were randomly divided into 7 groups, 6 rats in each group: C, D0, D24, D48, E0, E24, and E48. Each group of rats was killed and dissected at the different respective time points specified above. The whole quadriceps femoris of the left hind limbs were removed and divided into two parts, one for mRNAs of Beclin1, LC3 and PINK1 by real-time fluorescent quantitative PCR, and the other for LC3 protein by Western blotting. Results: Compared with group C, the contents of Beclin1 mRNA, PINK1 mRNA, and LC3 mRNA in the immediate exhaustive exercise group (E0) were significantly reduced p<0.01. However, the levels of PINK1 mRNA, LC3 mRNA, and LC3 protein in skeletal muscle cells increased significantly in the 48 hours after exhaustion (E48) p<0.05, suggesting that cell autophagy had an increasing trend during the recovery period. Meanwhile, compared with the C group, the contents of Beclin1 mRNA, PINK1 mRNA, and LC3 mRNA in the immediate blunt contusion group (D0) increased significantly p<0.01 and were followed by a downward trend. Conclusion: Generally, there were differences between the blunt contusion and exhausted exercise models at each recovery phase. The gene expression of the autophagy-related factors was not high in the early exhaustive exercise recovery phase and subsequently followed an upward trend. But the above factors increased significantly in the immediate and early recovery phases after blunt contusion. Injury from blunt contusion may be more severe than exhaustive exercise-induced-injury, so the autophagy starts earlier according to the changes in autophagy-related factors. Level of evidence III; Therapeutic studies investigating the results of treatment.Sociedade Brasileira de Medicina do Exercício e do Esporte2021-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-86922021000600563Revista Brasileira de Medicina do Esporte v.27 n.6 2021reponame:Revista brasileira de medicina do esporte (Online)instname:Sociedade Brasileira de Medicina do Exercício e do Esporte (SBMEE)instacron:SBMEE10.1590/1517-8692202127062020_0022info:eu-repo/semantics/openAccessPan,TongbinJi,MengjinJiao,JianjianYin,FengyangQin,ChengYang,Taoeng2021-11-11T00:00:00Zoai:scielo:S1517-86922021000600563Revistahttp://www.scielo.br/rbmeONGhttps://old.scielo.br/oai/scielo-oai.php||revista@medicinadoesporte.org.br1806-99401517-8692opendoar:2021-11-11T00:00Revista brasileira de medicina do esporte (Online) - Sociedade Brasileira de Medicina do Exercício e do Esporte (SBMEE)false |
dc.title.none.fl_str_mv |
EFFECTS OF EXHAUSTIVE EXERCISE AND CONTUSION ON AUTOPHAGY-RELATED FACTORS IN SKELETAL MUSCLE OF RATS |
title |
EFFECTS OF EXHAUSTIVE EXERCISE AND CONTUSION ON AUTOPHAGY-RELATED FACTORS IN SKELETAL MUSCLE OF RATS |
spellingShingle |
EFFECTS OF EXHAUSTIVE EXERCISE AND CONTUSION ON AUTOPHAGY-RELATED FACTORS IN SKELETAL MUSCLE OF RATS Pan,Tongbin Contusion Beclin1 Exercise |
title_short |
EFFECTS OF EXHAUSTIVE EXERCISE AND CONTUSION ON AUTOPHAGY-RELATED FACTORS IN SKELETAL MUSCLE OF RATS |
title_full |
EFFECTS OF EXHAUSTIVE EXERCISE AND CONTUSION ON AUTOPHAGY-RELATED FACTORS IN SKELETAL MUSCLE OF RATS |
title_fullStr |
EFFECTS OF EXHAUSTIVE EXERCISE AND CONTUSION ON AUTOPHAGY-RELATED FACTORS IN SKELETAL MUSCLE OF RATS |
title_full_unstemmed |
EFFECTS OF EXHAUSTIVE EXERCISE AND CONTUSION ON AUTOPHAGY-RELATED FACTORS IN SKELETAL MUSCLE OF RATS |
title_sort |
EFFECTS OF EXHAUSTIVE EXERCISE AND CONTUSION ON AUTOPHAGY-RELATED FACTORS IN SKELETAL MUSCLE OF RATS |
author |
Pan,Tongbin |
author_facet |
Pan,Tongbin Ji,Mengjin Jiao,Jianjian Yin,Fengyang Qin,Cheng Yang,Tao |
author_role |
author |
author2 |
Ji,Mengjin Jiao,Jianjian Yin,Fengyang Qin,Cheng Yang,Tao |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Pan,Tongbin Ji,Mengjin Jiao,Jianjian Yin,Fengyang Qin,Cheng Yang,Tao |
dc.subject.por.fl_str_mv |
Contusion Beclin1 Exercise |
topic |
Contusion Beclin1 Exercise |
description |
ABSTRACT Objective: To study the effects of exhaustive exercise and contusion on autophagy-related factors Beclin1, LC3 and PINK1 expression in the skeletal muscle of rats. Methods: Forty-two male SD rats were randomly divided into 7 groups, 6 rats in each group: C, D0, D24, D48, E0, E24, and E48. Each group of rats was killed and dissected at the different respective time points specified above. The whole quadriceps femoris of the left hind limbs were removed and divided into two parts, one for mRNAs of Beclin1, LC3 and PINK1 by real-time fluorescent quantitative PCR, and the other for LC3 protein by Western blotting. Results: Compared with group C, the contents of Beclin1 mRNA, PINK1 mRNA, and LC3 mRNA in the immediate exhaustive exercise group (E0) were significantly reduced p<0.01. However, the levels of PINK1 mRNA, LC3 mRNA, and LC3 protein in skeletal muscle cells increased significantly in the 48 hours after exhaustion (E48) p<0.05, suggesting that cell autophagy had an increasing trend during the recovery period. Meanwhile, compared with the C group, the contents of Beclin1 mRNA, PINK1 mRNA, and LC3 mRNA in the immediate blunt contusion group (D0) increased significantly p<0.01 and were followed by a downward trend. Conclusion: Generally, there were differences between the blunt contusion and exhausted exercise models at each recovery phase. The gene expression of the autophagy-related factors was not high in the early exhaustive exercise recovery phase and subsequently followed an upward trend. But the above factors increased significantly in the immediate and early recovery phases after blunt contusion. Injury from blunt contusion may be more severe than exhaustive exercise-induced-injury, so the autophagy starts earlier according to the changes in autophagy-related factors. Level of evidence III; Therapeutic studies investigating the results of treatment. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-86922021000600563 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-86922021000600563 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1517-8692202127062020_0022 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Medicina do Exercício e do Esporte |
publisher.none.fl_str_mv |
Sociedade Brasileira de Medicina do Exercício e do Esporte |
dc.source.none.fl_str_mv |
Revista Brasileira de Medicina do Esporte v.27 n.6 2021 reponame:Revista brasileira de medicina do esporte (Online) instname:Sociedade Brasileira de Medicina do Exercício e do Esporte (SBMEE) instacron:SBMEE |
instname_str |
Sociedade Brasileira de Medicina do Exercício e do Esporte (SBMEE) |
instacron_str |
SBMEE |
institution |
SBMEE |
reponame_str |
Revista brasileira de medicina do esporte (Online) |
collection |
Revista brasileira de medicina do esporte (Online) |
repository.name.fl_str_mv |
Revista brasileira de medicina do esporte (Online) - Sociedade Brasileira de Medicina do Exercício e do Esporte (SBMEE) |
repository.mail.fl_str_mv |
||revista@medicinadoesporte.org.br |
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1752122237731733504 |