Rapid detection of Van genes in rectal swabs by real time PCR in Southern Brazil

• Autor(a) principal: Cantarelli,Vlademir
• Data de Publicação: 2011
• Outros Autores: Cavalcante,Bianca, Pilger,Diogo André, Souza,Fabiana, Dias,Cícero Gomes, Brodt,Teresa, Cantarelli,Maritza, Secchi,Carina, d'Azevedo,Pedro Alves
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Revista da Sociedade Brasileira de Medicina Tropical
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000500021
• topic: RT-PCR; Vancomycin resistant enterococci; Genes vanA-vanB

INTRODUCTION: Laboratory-based surveillance is an important component in the control of vancomycin resistant enterococci (VRE). METHODS: The study aimed to evaluate real-time polymerase chain reaction (RT-PCR) (genes vanA-vanB) for VRE detection on 115 swabs from patients included in a surveillance program. RESULTS: Sensitivity of RT-PCR was similar to primary culture (75% and 79.5%, respectively) when compared to broth enriched culture, whereas specificity was 83.1%. CONCLUSIONS: RT-PCR provides same day results, however it showed low sensitivity for VRE detection.