Cytokine expression in the duodenal mucosa of patients with visceral leishmaniasis

Detalhes bibliográficos
Autor(a) principal: Luz,Kleber Giovanni
Data de Publicação: 2010
Outros Autores: Tuon,Felipe Francisco, Duarte,Maria Irma Seixas, Maia,Guilherme Mariz, Matos,Paulo, Ramos,Ana Maria de Oliveira, Nicodemo,Antônio Carlos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista da Sociedade Brasileira de Medicina Tropical
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822010000400011
Resumo: INTRODUCTION: Visceral leishmaniasis (VL) is a neglected tropical disease with a complex immune response in different organs. This pattern of organ-specific immune response has never been evaluated in the gastrointestinal tract. The aim of this study was to determine the in situ immune response in duodenal biopsies on patients with VL. METHODS: A case-control study was conducted on 13 patients with VL in comparison with nine controls. The immune response was evaluated using immunohistochemistry, for CD4, CD8, CD68, IL-4, IFN-γ, TNF-α and IL-10. Histological findings from the villi, crypts and inflammatory process were analyzed. RESULTS: All the cases of VL presented Leishmania antigens. No antigen was detected in the control group. The villus size was greater in the VL patients (p < 0.05). CD68 (macrophages) and CD4 levels were higher in the VL patients (p < 0.05). No differences in the expression of CD8, TNF-α, IL-10 or IL-4 were demonstrated. The number of cells expressing IFN-γ was lower in the VL patients (p < 0.05). CONCLUSIONS: Low levels of cytokines were found in the gastrointestinal tract of patients with VL. This pattern was not found in other organs affected by the disease. Immunotolerance of this tissue against Leishmania could explain these findings, as occurs with intestinal bacteria.
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spelling Cytokine expression in the duodenal mucosa of patients with visceral leishmaniasisVisceral leishmaniasisLeishmaniasisLeishmaniaINTRODUCTION: Visceral leishmaniasis (VL) is a neglected tropical disease with a complex immune response in different organs. This pattern of organ-specific immune response has never been evaluated in the gastrointestinal tract. The aim of this study was to determine the in situ immune response in duodenal biopsies on patients with VL. METHODS: A case-control study was conducted on 13 patients with VL in comparison with nine controls. The immune response was evaluated using immunohistochemistry, for CD4, CD8, CD68, IL-4, IFN-γ, TNF-α and IL-10. Histological findings from the villi, crypts and inflammatory process were analyzed. RESULTS: All the cases of VL presented Leishmania antigens. No antigen was detected in the control group. The villus size was greater in the VL patients (p < 0.05). CD68 (macrophages) and CD4 levels were higher in the VL patients (p < 0.05). No differences in the expression of CD8, TNF-α, IL-10 or IL-4 were demonstrated. The number of cells expressing IFN-γ was lower in the VL patients (p < 0.05). CONCLUSIONS: Low levels of cytokines were found in the gastrointestinal tract of patients with VL. This pattern was not found in other organs affected by the disease. Immunotolerance of this tissue against Leishmania could explain these findings, as occurs with intestinal bacteria.Sociedade Brasileira de Medicina Tropical - SBMT2010-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822010000400011Revista da Sociedade Brasileira de Medicina Tropical v.43 n.4 2010reponame:Revista da Sociedade Brasileira de Medicina Tropicalinstname:Sociedade Brasileira de Medicina Tropical (SBMT)instacron:SBMT10.1590/S0037-86822010000400011info:eu-repo/semantics/openAccessLuz,Kleber GiovanniTuon,Felipe FranciscoDuarte,Maria Irma SeixasMaia,Guilherme MarizMatos,PauloRamos,Ana Maria de OliveiraNicodemo,Antônio Carloseng2010-08-17T00:00:00Zoai:scielo:S0037-86822010000400011Revistahttps://www.sbmt.org.br/portal/revista/ONGhttps://old.scielo.br/oai/scielo-oai.php||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br1678-98490037-8682opendoar:2010-08-17T00:00Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT)false
dc.title.none.fl_str_mv Cytokine expression in the duodenal mucosa of patients with visceral leishmaniasis
title Cytokine expression in the duodenal mucosa of patients with visceral leishmaniasis
spellingShingle Cytokine expression in the duodenal mucosa of patients with visceral leishmaniasis
Luz,Kleber Giovanni
Visceral leishmaniasis
Leishmaniasis
Leishmania
title_short Cytokine expression in the duodenal mucosa of patients with visceral leishmaniasis
title_full Cytokine expression in the duodenal mucosa of patients with visceral leishmaniasis
title_fullStr Cytokine expression in the duodenal mucosa of patients with visceral leishmaniasis
title_full_unstemmed Cytokine expression in the duodenal mucosa of patients with visceral leishmaniasis
title_sort Cytokine expression in the duodenal mucosa of patients with visceral leishmaniasis
author Luz,Kleber Giovanni
author_facet Luz,Kleber Giovanni
Tuon,Felipe Francisco
Duarte,Maria Irma Seixas
Maia,Guilherme Mariz
Matos,Paulo
Ramos,Ana Maria de Oliveira
Nicodemo,Antônio Carlos
author_role author
author2 Tuon,Felipe Francisco
Duarte,Maria Irma Seixas
Maia,Guilherme Mariz
Matos,Paulo
Ramos,Ana Maria de Oliveira
Nicodemo,Antônio Carlos
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Luz,Kleber Giovanni
Tuon,Felipe Francisco
Duarte,Maria Irma Seixas
Maia,Guilherme Mariz
Matos,Paulo
Ramos,Ana Maria de Oliveira
Nicodemo,Antônio Carlos
dc.subject.por.fl_str_mv Visceral leishmaniasis
Leishmaniasis
Leishmania
topic Visceral leishmaniasis
Leishmaniasis
Leishmania
description INTRODUCTION: Visceral leishmaniasis (VL) is a neglected tropical disease with a complex immune response in different organs. This pattern of organ-specific immune response has never been evaluated in the gastrointestinal tract. The aim of this study was to determine the in situ immune response in duodenal biopsies on patients with VL. METHODS: A case-control study was conducted on 13 patients with VL in comparison with nine controls. The immune response was evaluated using immunohistochemistry, for CD4, CD8, CD68, IL-4, IFN-γ, TNF-α and IL-10. Histological findings from the villi, crypts and inflammatory process were analyzed. RESULTS: All the cases of VL presented Leishmania antigens. No antigen was detected in the control group. The villus size was greater in the VL patients (p < 0.05). CD68 (macrophages) and CD4 levels were higher in the VL patients (p < 0.05). No differences in the expression of CD8, TNF-α, IL-10 or IL-4 were demonstrated. The number of cells expressing IFN-γ was lower in the VL patients (p < 0.05). CONCLUSIONS: Low levels of cytokines were found in the gastrointestinal tract of patients with VL. This pattern was not found in other organs affected by the disease. Immunotolerance of this tissue against Leishmania could explain these findings, as occurs with intestinal bacteria.
publishDate 2010
dc.date.none.fl_str_mv 2010-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822010000400011
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dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0037-86822010000400011
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical - SBMT
publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical - SBMT
dc.source.none.fl_str_mv Revista da Sociedade Brasileira de Medicina Tropical v.43 n.4 2010
reponame:Revista da Sociedade Brasileira de Medicina Tropical
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repository.name.fl_str_mv Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT)
repository.mail.fl_str_mv ||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br
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