Flow cytometric quantitation of phagocytosis in heparinized complete blood with latex particles and Candida albicans

Detalhes bibliográficos
Autor(a) principal: Egido,Jesús M.
Data de Publicação: 1997
Outros Autores: Viñuelas,Juan
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista da Sociedade Brasileira de Medicina Tropical
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86821997000600001
Resumo: We report a rapid method for the flow cytometric quantitation of phagocytosis in heparinized complete peripherial blood (HCPB), using commercially available phycoerythrin-conjugated latex particles of 1µm diameter. The method is faster and shows greater reproducibility than Bjerknes' (1984) standard technique using propidium iodide-stained Candida albicans, conventionally applied to the leukocytic layer of peripherial blood but here modified for HCPB. We also report a modification of Bjerknes' Intracellular Killing Test to allow its application to HCPB.
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spelling Flow cytometric quantitation of phagocytosis in heparinized complete blood with latex particles and Candida albicansPhagocytosisFlow cytometryLatex particlesCandida albicansWe report a rapid method for the flow cytometric quantitation of phagocytosis in heparinized complete peripherial blood (HCPB), using commercially available phycoerythrin-conjugated latex particles of 1µm diameter. The method is faster and shows greater reproducibility than Bjerknes' (1984) standard technique using propidium iodide-stained Candida albicans, conventionally applied to the leukocytic layer of peripherial blood but here modified for HCPB. We also report a modification of Bjerknes' Intracellular Killing Test to allow its application to HCPB.Sociedade Brasileira de Medicina Tropical - SBMT1997-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86821997000600001Revista da Sociedade Brasileira de Medicina Tropical v.30 n.6 1997reponame:Revista da Sociedade Brasileira de Medicina Tropicalinstname:Sociedade Brasileira de Medicina Tropical (SBMT)instacron:SBMT10.1590/S0037-86821997000600001info:eu-repo/semantics/openAccessEgido,Jesús M.Viñuelas,Juaneng2000-06-13T00:00:00Zoai:scielo:S0037-86821997000600001Revistahttps://www.sbmt.org.br/portal/revista/ONGhttps://old.scielo.br/oai/scielo-oai.php||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br1678-98490037-8682opendoar:2000-06-13T00:00Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT)false
dc.title.none.fl_str_mv Flow cytometric quantitation of phagocytosis in heparinized complete blood with latex particles and Candida albicans
title Flow cytometric quantitation of phagocytosis in heparinized complete blood with latex particles and Candida albicans
spellingShingle Flow cytometric quantitation of phagocytosis in heparinized complete blood with latex particles and Candida albicans
Egido,Jesús M.
Phagocytosis
Flow cytometry
Latex particles
Candida albicans
title_short Flow cytometric quantitation of phagocytosis in heparinized complete blood with latex particles and Candida albicans
title_full Flow cytometric quantitation of phagocytosis in heparinized complete blood with latex particles and Candida albicans
title_fullStr Flow cytometric quantitation of phagocytosis in heparinized complete blood with latex particles and Candida albicans
title_full_unstemmed Flow cytometric quantitation of phagocytosis in heparinized complete blood with latex particles and Candida albicans
title_sort Flow cytometric quantitation of phagocytosis in heparinized complete blood with latex particles and Candida albicans
author Egido,Jesús M.
author_facet Egido,Jesús M.
Viñuelas,Juan
author_role author
author2 Viñuelas,Juan
author2_role author
dc.contributor.author.fl_str_mv Egido,Jesús M.
Viñuelas,Juan
dc.subject.por.fl_str_mv Phagocytosis
Flow cytometry
Latex particles
Candida albicans
topic Phagocytosis
Flow cytometry
Latex particles
Candida albicans
description We report a rapid method for the flow cytometric quantitation of phagocytosis in heparinized complete peripherial blood (HCPB), using commercially available phycoerythrin-conjugated latex particles of 1µm diameter. The method is faster and shows greater reproducibility than Bjerknes' (1984) standard technique using propidium iodide-stained Candida albicans, conventionally applied to the leukocytic layer of peripherial blood but here modified for HCPB. We also report a modification of Bjerknes' Intracellular Killing Test to allow its application to HCPB.
publishDate 1997
dc.date.none.fl_str_mv 1997-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86821997000600001
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86821997000600001
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0037-86821997000600001
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical - SBMT
publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical - SBMT
dc.source.none.fl_str_mv Revista da Sociedade Brasileira de Medicina Tropical v.30 n.6 1997
reponame:Revista da Sociedade Brasileira de Medicina Tropical
instname:Sociedade Brasileira de Medicina Tropical (SBMT)
instacron:SBMT
instname_str Sociedade Brasileira de Medicina Tropical (SBMT)
instacron_str SBMT
institution SBMT
reponame_str Revista da Sociedade Brasileira de Medicina Tropical
collection Revista da Sociedade Brasileira de Medicina Tropical
repository.name.fl_str_mv Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT)
repository.mail.fl_str_mv ||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br
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