Diagnosis of human herpesvirus 6B primary infection by polymerase chain reaction in young children with exanthematic disease

Detalhes bibliográficos
Autor(a) principal: Magalhães,Ivna de Melo
Data de Publicação: 2011
Outros Autores: Martins,Rebeca Vasquez Novo, Vianna,Renata Oliveira, Oliveira,Solange Artimos, Cavalcanti,Silvia Maria Baeta
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista da Sociedade Brasileira de Medicina Tropical
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000300008
Resumo: INTRODUCTION: Exanthem subitum is a classical rash disease of early childhood caused by human herpesvirus 6B (HHV-6B). However, the rash is frequently misdiagnosed as that of either measles or rubella. METHODS: In this study, a nested multiplex polymerase chain reaction (PCR) was used to diagnose HHV-6B primary infection, differentiate it from infections caused by HHV-6A and compare it to antibody avidity tests. The samples were separated into case group and control group according to the results of the indirect immunofluorescence assay (IFA) technique. RESULTS: From the saliva samples analyzed, HHV-6A DNA was detected in 3.2% of the case group and in 2.6% of the control group. Regarding HHV-6B, PCR detected viral DNA in 4.8% of the case group and in 1.3% of the control group. Among the serum samples studied, a frequency of 1.7% was determined for HHV-6A in the case group and 1.2% in the control group. PCR did not detect HHV-6B DNA in serum samples. The sensitivity and specificity of the PCR technique ranged from 0% to 4.8% and 97.5% to 100%, respectively, compared to IFA. CONCLUSIONS: The PCR technique was not suitable for diagnosing primary infection by HHV-6B in children with exanthematic disease and should not substitute the IFA.
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spelling Diagnosis of human herpesvirus 6B primary infection by polymerase chain reaction in young children with exanthematic diseaseHuman herpesvirus 6Exanthem subitumMultiplex PCRIndirect immunofluorescence assayPrimary infectionINTRODUCTION: Exanthem subitum is a classical rash disease of early childhood caused by human herpesvirus 6B (HHV-6B). However, the rash is frequently misdiagnosed as that of either measles or rubella. METHODS: In this study, a nested multiplex polymerase chain reaction (PCR) was used to diagnose HHV-6B primary infection, differentiate it from infections caused by HHV-6A and compare it to antibody avidity tests. The samples were separated into case group and control group according to the results of the indirect immunofluorescence assay (IFA) technique. RESULTS: From the saliva samples analyzed, HHV-6A DNA was detected in 3.2% of the case group and in 2.6% of the control group. Regarding HHV-6B, PCR detected viral DNA in 4.8% of the case group and in 1.3% of the control group. Among the serum samples studied, a frequency of 1.7% was determined for HHV-6A in the case group and 1.2% in the control group. PCR did not detect HHV-6B DNA in serum samples. The sensitivity and specificity of the PCR technique ranged from 0% to 4.8% and 97.5% to 100%, respectively, compared to IFA. CONCLUSIONS: The PCR technique was not suitable for diagnosing primary infection by HHV-6B in children with exanthematic disease and should not substitute the IFA.Sociedade Brasileira de Medicina Tropical - SBMT2011-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000300008Revista da Sociedade Brasileira de Medicina Tropical v.44 n.3 2011reponame:Revista da Sociedade Brasileira de Medicina Tropicalinstname:Sociedade Brasileira de Medicina Tropical (SBMT)instacron:SBMT10.1590/S0037-86822011005000021info:eu-repo/semantics/openAccessMagalhães,Ivna de MeloMartins,Rebeca Vasquez NovoVianna,Renata OliveiraOliveira,Solange ArtimosCavalcanti,Silvia Maria Baetaeng2011-07-11T00:00:00Zoai:scielo:S0037-86822011000300008Revistahttps://www.sbmt.org.br/portal/revista/ONGhttps://old.scielo.br/oai/scielo-oai.php||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br1678-98490037-8682opendoar:2011-07-11T00:00Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT)false
dc.title.none.fl_str_mv Diagnosis of human herpesvirus 6B primary infection by polymerase chain reaction in young children with exanthematic disease
title Diagnosis of human herpesvirus 6B primary infection by polymerase chain reaction in young children with exanthematic disease
spellingShingle Diagnosis of human herpesvirus 6B primary infection by polymerase chain reaction in young children with exanthematic disease
Magalhães,Ivna de Melo
Human herpesvirus 6
Exanthem subitum
Multiplex PCR
Indirect immunofluorescence assay
Primary infection
title_short Diagnosis of human herpesvirus 6B primary infection by polymerase chain reaction in young children with exanthematic disease
title_full Diagnosis of human herpesvirus 6B primary infection by polymerase chain reaction in young children with exanthematic disease
title_fullStr Diagnosis of human herpesvirus 6B primary infection by polymerase chain reaction in young children with exanthematic disease
title_full_unstemmed Diagnosis of human herpesvirus 6B primary infection by polymerase chain reaction in young children with exanthematic disease
title_sort Diagnosis of human herpesvirus 6B primary infection by polymerase chain reaction in young children with exanthematic disease
author Magalhães,Ivna de Melo
author_facet Magalhães,Ivna de Melo
Martins,Rebeca Vasquez Novo
Vianna,Renata Oliveira
Oliveira,Solange Artimos
Cavalcanti,Silvia Maria Baeta
author_role author
author2 Martins,Rebeca Vasquez Novo
Vianna,Renata Oliveira
Oliveira,Solange Artimos
Cavalcanti,Silvia Maria Baeta
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Magalhães,Ivna de Melo
Martins,Rebeca Vasquez Novo
Vianna,Renata Oliveira
Oliveira,Solange Artimos
Cavalcanti,Silvia Maria Baeta
dc.subject.por.fl_str_mv Human herpesvirus 6
Exanthem subitum
Multiplex PCR
Indirect immunofluorescence assay
Primary infection
topic Human herpesvirus 6
Exanthem subitum
Multiplex PCR
Indirect immunofluorescence assay
Primary infection
description INTRODUCTION: Exanthem subitum is a classical rash disease of early childhood caused by human herpesvirus 6B (HHV-6B). However, the rash is frequently misdiagnosed as that of either measles or rubella. METHODS: In this study, a nested multiplex polymerase chain reaction (PCR) was used to diagnose HHV-6B primary infection, differentiate it from infections caused by HHV-6A and compare it to antibody avidity tests. The samples were separated into case group and control group according to the results of the indirect immunofluorescence assay (IFA) technique. RESULTS: From the saliva samples analyzed, HHV-6A DNA was detected in 3.2% of the case group and in 2.6% of the control group. Regarding HHV-6B, PCR detected viral DNA in 4.8% of the case group and in 1.3% of the control group. Among the serum samples studied, a frequency of 1.7% was determined for HHV-6A in the case group and 1.2% in the control group. PCR did not detect HHV-6B DNA in serum samples. The sensitivity and specificity of the PCR technique ranged from 0% to 4.8% and 97.5% to 100%, respectively, compared to IFA. CONCLUSIONS: The PCR technique was not suitable for diagnosing primary infection by HHV-6B in children with exanthematic disease and should not substitute the IFA.
publishDate 2011
dc.date.none.fl_str_mv 2011-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000300008
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dc.language.iso.fl_str_mv eng
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dc.relation.none.fl_str_mv 10.1590/S0037-86822011005000021
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical - SBMT
publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical - SBMT
dc.source.none.fl_str_mv Revista da Sociedade Brasileira de Medicina Tropical v.44 n.3 2011
reponame:Revista da Sociedade Brasileira de Medicina Tropical
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reponame_str Revista da Sociedade Brasileira de Medicina Tropical
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repository.name.fl_str_mv Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT)
repository.mail.fl_str_mv ||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br
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