Diagnosis of human herpesvirus 6B primary infection by polymerase chain reaction in young children with exanthematic disease
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista da Sociedade Brasileira de Medicina Tropical |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000300008 |
Resumo: | INTRODUCTION: Exanthem subitum is a classical rash disease of early childhood caused by human herpesvirus 6B (HHV-6B). However, the rash is frequently misdiagnosed as that of either measles or rubella. METHODS: In this study, a nested multiplex polymerase chain reaction (PCR) was used to diagnose HHV-6B primary infection, differentiate it from infections caused by HHV-6A and compare it to antibody avidity tests. The samples were separated into case group and control group according to the results of the indirect immunofluorescence assay (IFA) technique. RESULTS: From the saliva samples analyzed, HHV-6A DNA was detected in 3.2% of the case group and in 2.6% of the control group. Regarding HHV-6B, PCR detected viral DNA in 4.8% of the case group and in 1.3% of the control group. Among the serum samples studied, a frequency of 1.7% was determined for HHV-6A in the case group and 1.2% in the control group. PCR did not detect HHV-6B DNA in serum samples. The sensitivity and specificity of the PCR technique ranged from 0% to 4.8% and 97.5% to 100%, respectively, compared to IFA. CONCLUSIONS: The PCR technique was not suitable for diagnosing primary infection by HHV-6B in children with exanthematic disease and should not substitute the IFA. |
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Diagnosis of human herpesvirus 6B primary infection by polymerase chain reaction in young children with exanthematic diseaseHuman herpesvirus 6Exanthem subitumMultiplex PCRIndirect immunofluorescence assayPrimary infectionINTRODUCTION: Exanthem subitum is a classical rash disease of early childhood caused by human herpesvirus 6B (HHV-6B). However, the rash is frequently misdiagnosed as that of either measles or rubella. METHODS: In this study, a nested multiplex polymerase chain reaction (PCR) was used to diagnose HHV-6B primary infection, differentiate it from infections caused by HHV-6A and compare it to antibody avidity tests. The samples were separated into case group and control group according to the results of the indirect immunofluorescence assay (IFA) technique. RESULTS: From the saliva samples analyzed, HHV-6A DNA was detected in 3.2% of the case group and in 2.6% of the control group. Regarding HHV-6B, PCR detected viral DNA in 4.8% of the case group and in 1.3% of the control group. Among the serum samples studied, a frequency of 1.7% was determined for HHV-6A in the case group and 1.2% in the control group. PCR did not detect HHV-6B DNA in serum samples. The sensitivity and specificity of the PCR technique ranged from 0% to 4.8% and 97.5% to 100%, respectively, compared to IFA. CONCLUSIONS: The PCR technique was not suitable for diagnosing primary infection by HHV-6B in children with exanthematic disease and should not substitute the IFA.Sociedade Brasileira de Medicina Tropical - SBMT2011-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000300008Revista da Sociedade Brasileira de Medicina Tropical v.44 n.3 2011reponame:Revista da Sociedade Brasileira de Medicina Tropicalinstname:Sociedade Brasileira de Medicina Tropical (SBMT)instacron:SBMT10.1590/S0037-86822011005000021info:eu-repo/semantics/openAccessMagalhães,Ivna de MeloMartins,Rebeca Vasquez NovoVianna,Renata OliveiraOliveira,Solange ArtimosCavalcanti,Silvia Maria Baetaeng2011-07-11T00:00:00Zoai:scielo:S0037-86822011000300008Revistahttps://www.sbmt.org.br/portal/revista/ONGhttps://old.scielo.br/oai/scielo-oai.php||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br1678-98490037-8682opendoar:2011-07-11T00:00Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT)false |
dc.title.none.fl_str_mv |
Diagnosis of human herpesvirus 6B primary infection by polymerase chain reaction in young children with exanthematic disease |
title |
Diagnosis of human herpesvirus 6B primary infection by polymerase chain reaction in young children with exanthematic disease |
spellingShingle |
Diagnosis of human herpesvirus 6B primary infection by polymerase chain reaction in young children with exanthematic disease Magalhães,Ivna de Melo Human herpesvirus 6 Exanthem subitum Multiplex PCR Indirect immunofluorescence assay Primary infection |
title_short |
Diagnosis of human herpesvirus 6B primary infection by polymerase chain reaction in young children with exanthematic disease |
title_full |
Diagnosis of human herpesvirus 6B primary infection by polymerase chain reaction in young children with exanthematic disease |
title_fullStr |
Diagnosis of human herpesvirus 6B primary infection by polymerase chain reaction in young children with exanthematic disease |
title_full_unstemmed |
Diagnosis of human herpesvirus 6B primary infection by polymerase chain reaction in young children with exanthematic disease |
title_sort |
Diagnosis of human herpesvirus 6B primary infection by polymerase chain reaction in young children with exanthematic disease |
author |
Magalhães,Ivna de Melo |
author_facet |
Magalhães,Ivna de Melo Martins,Rebeca Vasquez Novo Vianna,Renata Oliveira Oliveira,Solange Artimos Cavalcanti,Silvia Maria Baeta |
author_role |
author |
author2 |
Martins,Rebeca Vasquez Novo Vianna,Renata Oliveira Oliveira,Solange Artimos Cavalcanti,Silvia Maria Baeta |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Magalhães,Ivna de Melo Martins,Rebeca Vasquez Novo Vianna,Renata Oliveira Oliveira,Solange Artimos Cavalcanti,Silvia Maria Baeta |
dc.subject.por.fl_str_mv |
Human herpesvirus 6 Exanthem subitum Multiplex PCR Indirect immunofluorescence assay Primary infection |
topic |
Human herpesvirus 6 Exanthem subitum Multiplex PCR Indirect immunofluorescence assay Primary infection |
description |
INTRODUCTION: Exanthem subitum is a classical rash disease of early childhood caused by human herpesvirus 6B (HHV-6B). However, the rash is frequently misdiagnosed as that of either measles or rubella. METHODS: In this study, a nested multiplex polymerase chain reaction (PCR) was used to diagnose HHV-6B primary infection, differentiate it from infections caused by HHV-6A and compare it to antibody avidity tests. The samples were separated into case group and control group according to the results of the indirect immunofluorescence assay (IFA) technique. RESULTS: From the saliva samples analyzed, HHV-6A DNA was detected in 3.2% of the case group and in 2.6% of the control group. Regarding HHV-6B, PCR detected viral DNA in 4.8% of the case group and in 1.3% of the control group. Among the serum samples studied, a frequency of 1.7% was determined for HHV-6A in the case group and 1.2% in the control group. PCR did not detect HHV-6B DNA in serum samples. The sensitivity and specificity of the PCR technique ranged from 0% to 4.8% and 97.5% to 100%, respectively, compared to IFA. CONCLUSIONS: The PCR technique was not suitable for diagnosing primary infection by HHV-6B in children with exanthematic disease and should not substitute the IFA. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000300008 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000300008 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0037-86822011005000021 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Medicina Tropical - SBMT |
publisher.none.fl_str_mv |
Sociedade Brasileira de Medicina Tropical - SBMT |
dc.source.none.fl_str_mv |
Revista da Sociedade Brasileira de Medicina Tropical v.44 n.3 2011 reponame:Revista da Sociedade Brasileira de Medicina Tropical instname:Sociedade Brasileira de Medicina Tropical (SBMT) instacron:SBMT |
instname_str |
Sociedade Brasileira de Medicina Tropical (SBMT) |
instacron_str |
SBMT |
institution |
SBMT |
reponame_str |
Revista da Sociedade Brasileira de Medicina Tropical |
collection |
Revista da Sociedade Brasileira de Medicina Tropical |
repository.name.fl_str_mv |
Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT) |
repository.mail.fl_str_mv |
||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br |
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1752122157208436736 |