Natural infection by Trypanosoma cruzi in triatomines and seropositivity for Chagas disease of dogs in rural areas of Rio Grande do Norte, Brazil

Detalhes bibliográficos
Autor(a) principal: Freitas,Yannara Barbosa Nogueira
Data de Publicação: 2018
Outros Autores: Souza,Celeste da Silva Freitas de, Magalhães,Jamille Maia e, Sousa,Maressa Laíse Reginaldo de, d’Escoffier,Luiz Ney, Valle,Tânia Zaverucha do, Gonçalves,Teresa Cristina Monte, Gil-Santana,Hélcio Reinaldo, Kazimoto,Thais Aaparecida, Amora,Sthenia Santos Albano
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista da Sociedade Brasileira de Medicina Tropical
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822018000200190
Resumo: Abstract INTRODUCTION: Chagas disease is caused by the protozoa Trypanosoma cruzi. Its main reservoir is the domestic dog, especially in rural areas with favorable characteristics for vector establishment and proliferation. The aims of this study were to collect data, survey and map the fauna, and identify T. cruzi infection in triatomines, as well as to assess the presence of anti-T. cruzi antibodies in dogs in rural areas of the municipality of Mossoró, Brazil. METHODS: An active entomologic research was conducted to identify adult specimens through an external morphology dichotomous key. The analysis of natural infection by T. cruzi in the insects was performed by isolation in culture and polymerase chain reaction. The antibody testing for T. cruzi in dogs was performed by enzyme-linked immunosorbent assay and indirect immunofluorescence assay. RESULTS: A total of 68 triatomines were captured, predominantly the Triatoma brasiliensis brasiliensis (Neiva 1911) species. The vector mapping displayed areas with greater risk for parasite transmission. Of the examined triatomines (51 specimens), 41.2% (21/51) were positive on polymerase chain reaction, and all were negative on culture. In the serum testing, 11% (25/218) of dogs were seropositive, but no association was found between the serologic results and the presence and infection by T. cruzi in triatomines. CONCLUSIONS: This study demonstrated the movement of T. cruzi in the studied area, by the presence of vectors and naturally infected domestic reservoirs. The mapping of the studied rural area demonstrates the risk of disease transmission.
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spelling Natural infection by Trypanosoma cruzi in triatomines and seropositivity for Chagas disease of dogs in rural areas of Rio Grande do Norte, BrazilChagas diseaseVectorDomestic reservoirDiagnosisZoonosisAbstract INTRODUCTION: Chagas disease is caused by the protozoa Trypanosoma cruzi. Its main reservoir is the domestic dog, especially in rural areas with favorable characteristics for vector establishment and proliferation. The aims of this study were to collect data, survey and map the fauna, and identify T. cruzi infection in triatomines, as well as to assess the presence of anti-T. cruzi antibodies in dogs in rural areas of the municipality of Mossoró, Brazil. METHODS: An active entomologic research was conducted to identify adult specimens through an external morphology dichotomous key. The analysis of natural infection by T. cruzi in the insects was performed by isolation in culture and polymerase chain reaction. The antibody testing for T. cruzi in dogs was performed by enzyme-linked immunosorbent assay and indirect immunofluorescence assay. RESULTS: A total of 68 triatomines were captured, predominantly the Triatoma brasiliensis brasiliensis (Neiva 1911) species. The vector mapping displayed areas with greater risk for parasite transmission. Of the examined triatomines (51 specimens), 41.2% (21/51) were positive on polymerase chain reaction, and all were negative on culture. In the serum testing, 11% (25/218) of dogs were seropositive, but no association was found between the serologic results and the presence and infection by T. cruzi in triatomines. CONCLUSIONS: This study demonstrated the movement of T. cruzi in the studied area, by the presence of vectors and naturally infected domestic reservoirs. The mapping of the studied rural area demonstrates the risk of disease transmission.Sociedade Brasileira de Medicina Tropical - SBMT2018-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822018000200190Revista da Sociedade Brasileira de Medicina Tropical v.51 n.2 2018reponame:Revista da Sociedade Brasileira de Medicina Tropicalinstname:Sociedade Brasileira de Medicina Tropical (SBMT)instacron:SBMT10.1590/0037-8682-0088-2017info:eu-repo/semantics/openAccessFreitas,Yannara Barbosa NogueiraSouza,Celeste da Silva Freitas deMagalhães,Jamille Maia eSousa,Maressa Laíse Reginaldo ded’Escoffier,Luiz NeyValle,Tânia Zaverucha doGonçalves,Teresa Cristina MonteGil-Santana,Hélcio ReinaldoKazimoto,Thais AaparecidaAmora,Sthenia Santos Albanoeng2018-05-11T00:00:00Zoai:scielo:S0037-86822018000200190Revistahttps://www.sbmt.org.br/portal/revista/ONGhttps://old.scielo.br/oai/scielo-oai.php||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br1678-98490037-8682opendoar:2018-05-11T00:00Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT)false
dc.title.none.fl_str_mv Natural infection by Trypanosoma cruzi in triatomines and seropositivity for Chagas disease of dogs in rural areas of Rio Grande do Norte, Brazil
title Natural infection by Trypanosoma cruzi in triatomines and seropositivity for Chagas disease of dogs in rural areas of Rio Grande do Norte, Brazil
spellingShingle Natural infection by Trypanosoma cruzi in triatomines and seropositivity for Chagas disease of dogs in rural areas of Rio Grande do Norte, Brazil
Freitas,Yannara Barbosa Nogueira
Chagas disease
Vector
Domestic reservoir
Diagnosis
Zoonosis
title_short Natural infection by Trypanosoma cruzi in triatomines and seropositivity for Chagas disease of dogs in rural areas of Rio Grande do Norte, Brazil
title_full Natural infection by Trypanosoma cruzi in triatomines and seropositivity for Chagas disease of dogs in rural areas of Rio Grande do Norte, Brazil
title_fullStr Natural infection by Trypanosoma cruzi in triatomines and seropositivity for Chagas disease of dogs in rural areas of Rio Grande do Norte, Brazil
title_full_unstemmed Natural infection by Trypanosoma cruzi in triatomines and seropositivity for Chagas disease of dogs in rural areas of Rio Grande do Norte, Brazil
title_sort Natural infection by Trypanosoma cruzi in triatomines and seropositivity for Chagas disease of dogs in rural areas of Rio Grande do Norte, Brazil
author Freitas,Yannara Barbosa Nogueira
author_facet Freitas,Yannara Barbosa Nogueira
Souza,Celeste da Silva Freitas de
Magalhães,Jamille Maia e
Sousa,Maressa Laíse Reginaldo de
d’Escoffier,Luiz Ney
Valle,Tânia Zaverucha do
Gonçalves,Teresa Cristina Monte
Gil-Santana,Hélcio Reinaldo
Kazimoto,Thais Aaparecida
Amora,Sthenia Santos Albano
author_role author
author2 Souza,Celeste da Silva Freitas de
Magalhães,Jamille Maia e
Sousa,Maressa Laíse Reginaldo de
d’Escoffier,Luiz Ney
Valle,Tânia Zaverucha do
Gonçalves,Teresa Cristina Monte
Gil-Santana,Hélcio Reinaldo
Kazimoto,Thais Aaparecida
Amora,Sthenia Santos Albano
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Freitas,Yannara Barbosa Nogueira
Souza,Celeste da Silva Freitas de
Magalhães,Jamille Maia e
Sousa,Maressa Laíse Reginaldo de
d’Escoffier,Luiz Ney
Valle,Tânia Zaverucha do
Gonçalves,Teresa Cristina Monte
Gil-Santana,Hélcio Reinaldo
Kazimoto,Thais Aaparecida
Amora,Sthenia Santos Albano
dc.subject.por.fl_str_mv Chagas disease
Vector
Domestic reservoir
Diagnosis
Zoonosis
topic Chagas disease
Vector
Domestic reservoir
Diagnosis
Zoonosis
description Abstract INTRODUCTION: Chagas disease is caused by the protozoa Trypanosoma cruzi. Its main reservoir is the domestic dog, especially in rural areas with favorable characteristics for vector establishment and proliferation. The aims of this study were to collect data, survey and map the fauna, and identify T. cruzi infection in triatomines, as well as to assess the presence of anti-T. cruzi antibodies in dogs in rural areas of the municipality of Mossoró, Brazil. METHODS: An active entomologic research was conducted to identify adult specimens through an external morphology dichotomous key. The analysis of natural infection by T. cruzi in the insects was performed by isolation in culture and polymerase chain reaction. The antibody testing for T. cruzi in dogs was performed by enzyme-linked immunosorbent assay and indirect immunofluorescence assay. RESULTS: A total of 68 triatomines were captured, predominantly the Triatoma brasiliensis brasiliensis (Neiva 1911) species. The vector mapping displayed areas with greater risk for parasite transmission. Of the examined triatomines (51 specimens), 41.2% (21/51) were positive on polymerase chain reaction, and all were negative on culture. In the serum testing, 11% (25/218) of dogs were seropositive, but no association was found between the serologic results and the presence and infection by T. cruzi in triatomines. CONCLUSIONS: This study demonstrated the movement of T. cruzi in the studied area, by the presence of vectors and naturally infected domestic reservoirs. The mapping of the studied rural area demonstrates the risk of disease transmission.
publishDate 2018
dc.date.none.fl_str_mv 2018-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822018000200190
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822018000200190
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0037-8682-0088-2017
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical - SBMT
publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical - SBMT
dc.source.none.fl_str_mv Revista da Sociedade Brasileira de Medicina Tropical v.51 n.2 2018
reponame:Revista da Sociedade Brasileira de Medicina Tropical
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reponame_str Revista da Sociedade Brasileira de Medicina Tropical
collection Revista da Sociedade Brasileira de Medicina Tropical
repository.name.fl_str_mv Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT)
repository.mail.fl_str_mv ||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br
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