Monitoring Trypanosoma cruzi infection in triatomines using PCR in Mato Grosso do Sul, Brazil

Detalhes bibliográficos
Autor(a) principal: Cominetti,Marlon Cezar
Data de Publicação: 2013
Outros Autores: Almeida,Robson Ferreira Cavalcante de, Goncalves,Guilmara Maria do Amaral, Andreotti,Renato
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista da Sociedade Brasileira de Medicina Tropical
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822013000300277
Resumo: Introduction The aim of the present study was to assess the polymerase chain reaction (PCR) as a method for detecting Trypanosoma cruzi infection in triatomines that had been previously determined by microscopic examination in the State of Mato Grosso do Sul, Brazil. Methods In total, 515 specimens were collected. Material from the digestive tract of each triatomine was analyzed for the presence of T. cruzi by microscopic examination and PCR using the 121/122 primer set. Results Among the 515 specimens tested, 58 (11.3%) were positive by microscopy and 101 (19.61%) were positive by PCR and there was an association between the results of the techniques (χ2 = 53.354, p = 0.001). The main species of triatomine identified was T. sordida (95.5%) Conclusions The use of PCR in entomological surveillance may contribute to a better assessment of the occurrence of T. cruzi in triatomine populations.
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spelling Monitoring Trypanosoma cruzi infection in triatomines using PCR in Mato Grosso do Sul, BrazilEpidemiologyTriatomine infection monitoringPolymerase chain reactionMicroscopy examinationTrypanosoma cruzi Introduction The aim of the present study was to assess the polymerase chain reaction (PCR) as a method for detecting Trypanosoma cruzi infection in triatomines that had been previously determined by microscopic examination in the State of Mato Grosso do Sul, Brazil. Methods In total, 515 specimens were collected. Material from the digestive tract of each triatomine was analyzed for the presence of T. cruzi by microscopic examination and PCR using the 121/122 primer set. Results Among the 515 specimens tested, 58 (11.3%) were positive by microscopy and 101 (19.61%) were positive by PCR and there was an association between the results of the techniques (χ2 = 53.354, p = 0.001). The main species of triatomine identified was T. sordida (95.5%) Conclusions The use of PCR in entomological surveillance may contribute to a better assessment of the occurrence of T. cruzi in triatomine populations. Sociedade Brasileira de Medicina Tropical - SBMT2013-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822013000300277Revista da Sociedade Brasileira de Medicina Tropical v.46 n.3 2013reponame:Revista da Sociedade Brasileira de Medicina Tropicalinstname:Sociedade Brasileira de Medicina Tropical (SBMT)instacron:SBMT10.1590/0037-8682-0024-2013info:eu-repo/semantics/openAccessCominetti,Marlon CezarAlmeida,Robson Ferreira Cavalcante deGoncalves,Guilmara Maria do AmaralAndreotti,Renatoeng2013-07-05T00:00:00Zoai:scielo:S0037-86822013000300277Revistahttps://www.sbmt.org.br/portal/revista/ONGhttps://old.scielo.br/oai/scielo-oai.php||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br1678-98490037-8682opendoar:2013-07-05T00:00Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT)false
dc.title.none.fl_str_mv Monitoring Trypanosoma cruzi infection in triatomines using PCR in Mato Grosso do Sul, Brazil
title Monitoring Trypanosoma cruzi infection in triatomines using PCR in Mato Grosso do Sul, Brazil
spellingShingle Monitoring Trypanosoma cruzi infection in triatomines using PCR in Mato Grosso do Sul, Brazil
Cominetti,Marlon Cezar
Epidemiology
Triatomine infection monitoring
Polymerase chain reaction
Microscopy examination
Trypanosoma cruzi
title_short Monitoring Trypanosoma cruzi infection in triatomines using PCR in Mato Grosso do Sul, Brazil
title_full Monitoring Trypanosoma cruzi infection in triatomines using PCR in Mato Grosso do Sul, Brazil
title_fullStr Monitoring Trypanosoma cruzi infection in triatomines using PCR in Mato Grosso do Sul, Brazil
title_full_unstemmed Monitoring Trypanosoma cruzi infection in triatomines using PCR in Mato Grosso do Sul, Brazil
title_sort Monitoring Trypanosoma cruzi infection in triatomines using PCR in Mato Grosso do Sul, Brazil
author Cominetti,Marlon Cezar
author_facet Cominetti,Marlon Cezar
Almeida,Robson Ferreira Cavalcante de
Goncalves,Guilmara Maria do Amaral
Andreotti,Renato
author_role author
author2 Almeida,Robson Ferreira Cavalcante de
Goncalves,Guilmara Maria do Amaral
Andreotti,Renato
author2_role author
author
author
dc.contributor.author.fl_str_mv Cominetti,Marlon Cezar
Almeida,Robson Ferreira Cavalcante de
Goncalves,Guilmara Maria do Amaral
Andreotti,Renato
dc.subject.por.fl_str_mv Epidemiology
Triatomine infection monitoring
Polymerase chain reaction
Microscopy examination
Trypanosoma cruzi
topic Epidemiology
Triatomine infection monitoring
Polymerase chain reaction
Microscopy examination
Trypanosoma cruzi
description Introduction The aim of the present study was to assess the polymerase chain reaction (PCR) as a method for detecting Trypanosoma cruzi infection in triatomines that had been previously determined by microscopic examination in the State of Mato Grosso do Sul, Brazil. Methods In total, 515 specimens were collected. Material from the digestive tract of each triatomine was analyzed for the presence of T. cruzi by microscopic examination and PCR using the 121/122 primer set. Results Among the 515 specimens tested, 58 (11.3%) were positive by microscopy and 101 (19.61%) were positive by PCR and there was an association between the results of the techniques (χ2 = 53.354, p = 0.001). The main species of triatomine identified was T. sordida (95.5%) Conclusions The use of PCR in entomological surveillance may contribute to a better assessment of the occurrence of T. cruzi in triatomine populations.
publishDate 2013
dc.date.none.fl_str_mv 2013-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822013000300277
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822013000300277
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0037-8682-0024-2013
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical - SBMT
publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical - SBMT
dc.source.none.fl_str_mv Revista da Sociedade Brasileira de Medicina Tropical v.46 n.3 2013
reponame:Revista da Sociedade Brasileira de Medicina Tropical
instname:Sociedade Brasileira de Medicina Tropical (SBMT)
instacron:SBMT
instname_str Sociedade Brasileira de Medicina Tropical (SBMT)
instacron_str SBMT
institution SBMT
reponame_str Revista da Sociedade Brasileira de Medicina Tropical
collection Revista da Sociedade Brasileira de Medicina Tropical
repository.name.fl_str_mv Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT)
repository.mail.fl_str_mv ||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br
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