Hemocultures for the parasitological diagnosis of human chronic Chagas' disease

Detalhes bibliográficos
Autor(a) principal: Chiari,Egler
Data de Publicação: 1989
Outros Autores: Dias,João Carlos Pinto, Lana,Marta, Chiari,Clea Andrade
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista da Sociedade Brasileira de Medicina Tropical
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86821989000100004
Resumo: With the purpose of standardization of an hemoculture technique presenting a higher positive rate in the parasitological diagnosis of chronic Chagas' disease in patients with reactive serology (IFT, HA, CFT) the following schedule was used. Thirty ml of venous blood was collected with heparin and the plasma was separated by centrifugation (2.000 rpm/30'). The packed cells were washed with LIT medium or PBS which was then removed by centrifugation (2.000 rpm/15'). This material was sampled in 6 screw-tubes 18x200 with 6 ml of LIT medium and incubated at 28°C. These incubated cultures at 28°C were examined after 15, 30, 45 and 60 days. When the hemoculture was not immediately processed after blood collection, the plasma was removed and the sediment enriched with LIT medium and preserved at 4°C. The Xenodiagnosis was performed according to Schenones method used here as a reference technique. Among the various groups of patients examined by both techniques the best results obtained were: 55.08% ofpositivity for hemocultures against 27.5% forxenodiagnosis (X² = 4.54, p = 0.05), with a tubepositivity of 26.6%. Recommendation for screening trials of drug assays is the repetition of method on a same patient 2 or more times in different occasions, as used in xenodiagnosis.
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spelling Hemocultures for the parasitological diagnosis of human chronic Chagas' diseaseChagas' diseaseParasitological diagnosisHemoculture and xenodiagnosisTrypanosoma cruziWith the purpose of standardization of an hemoculture technique presenting a higher positive rate in the parasitological diagnosis of chronic Chagas' disease in patients with reactive serology (IFT, HA, CFT) the following schedule was used. Thirty ml of venous blood was collected with heparin and the plasma was separated by centrifugation (2.000 rpm/30'). The packed cells were washed with LIT medium or PBS which was then removed by centrifugation (2.000 rpm/15'). This material was sampled in 6 screw-tubes 18x200 with 6 ml of LIT medium and incubated at 28°C. These incubated cultures at 28°C were examined after 15, 30, 45 and 60 days. When the hemoculture was not immediately processed after blood collection, the plasma was removed and the sediment enriched with LIT medium and preserved at 4°C. The Xenodiagnosis was performed according to Schenones method used here as a reference technique. Among the various groups of patients examined by both techniques the best results obtained were: 55.08% ofpositivity for hemocultures against 27.5% forxenodiagnosis (X² = 4.54, p = 0.05), with a tubepositivity of 26.6%. Recommendation for screening trials of drug assays is the repetition of method on a same patient 2 or more times in different occasions, as used in xenodiagnosis.Sociedade Brasileira de Medicina Tropical - SBMT1989-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86821989000100004Revista da Sociedade Brasileira de Medicina Tropical v.22 n.1 1989reponame:Revista da Sociedade Brasileira de Medicina Tropicalinstname:Sociedade Brasileira de Medicina Tropical (SBMT)instacron:SBMT10.1590/S0037-86821989000100004info:eu-repo/semantics/openAccessChiari,EglerDias,João Carlos PintoLana,MartaChiari,Clea Andradeeng2013-05-27T00:00:00Zoai:scielo:S0037-86821989000100004Revistahttps://www.sbmt.org.br/portal/revista/ONGhttps://old.scielo.br/oai/scielo-oai.php||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br1678-98490037-8682opendoar:2013-05-27T00:00Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT)false
dc.title.none.fl_str_mv Hemocultures for the parasitological diagnosis of human chronic Chagas' disease
title Hemocultures for the parasitological diagnosis of human chronic Chagas' disease
spellingShingle Hemocultures for the parasitological diagnosis of human chronic Chagas' disease
Chiari,Egler
Chagas' disease
Parasitological diagnosis
Hemoculture and xenodiagnosis
Trypanosoma cruzi
title_short Hemocultures for the parasitological diagnosis of human chronic Chagas' disease
title_full Hemocultures for the parasitological diagnosis of human chronic Chagas' disease
title_fullStr Hemocultures for the parasitological diagnosis of human chronic Chagas' disease
title_full_unstemmed Hemocultures for the parasitological diagnosis of human chronic Chagas' disease
title_sort Hemocultures for the parasitological diagnosis of human chronic Chagas' disease
author Chiari,Egler
author_facet Chiari,Egler
Dias,João Carlos Pinto
Lana,Marta
Chiari,Clea Andrade
author_role author
author2 Dias,João Carlos Pinto
Lana,Marta
Chiari,Clea Andrade
author2_role author
author
author
dc.contributor.author.fl_str_mv Chiari,Egler
Dias,João Carlos Pinto
Lana,Marta
Chiari,Clea Andrade
dc.subject.por.fl_str_mv Chagas' disease
Parasitological diagnosis
Hemoculture and xenodiagnosis
Trypanosoma cruzi
topic Chagas' disease
Parasitological diagnosis
Hemoculture and xenodiagnosis
Trypanosoma cruzi
description With the purpose of standardization of an hemoculture technique presenting a higher positive rate in the parasitological diagnosis of chronic Chagas' disease in patients with reactive serology (IFT, HA, CFT) the following schedule was used. Thirty ml of venous blood was collected with heparin and the plasma was separated by centrifugation (2.000 rpm/30'). The packed cells were washed with LIT medium or PBS which was then removed by centrifugation (2.000 rpm/15'). This material was sampled in 6 screw-tubes 18x200 with 6 ml of LIT medium and incubated at 28°C. These incubated cultures at 28°C were examined after 15, 30, 45 and 60 days. When the hemoculture was not immediately processed after blood collection, the plasma was removed and the sediment enriched with LIT medium and preserved at 4°C. The Xenodiagnosis was performed according to Schenones method used here as a reference technique. Among the various groups of patients examined by both techniques the best results obtained were: 55.08% ofpositivity for hemocultures against 27.5% forxenodiagnosis (X² = 4.54, p = 0.05), with a tubepositivity of 26.6%. Recommendation for screening trials of drug assays is the repetition of method on a same patient 2 or more times in different occasions, as used in xenodiagnosis.
publishDate 1989
dc.date.none.fl_str_mv 1989-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86821989000100004
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86821989000100004
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0037-86821989000100004
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dc.publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical - SBMT
publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical - SBMT
dc.source.none.fl_str_mv Revista da Sociedade Brasileira de Medicina Tropical v.22 n.1 1989
reponame:Revista da Sociedade Brasileira de Medicina Tropical
instname:Sociedade Brasileira de Medicina Tropical (SBMT)
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repository.name.fl_str_mv Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT)
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