Detection of Maedi visna virus, by Nested-PCR, in bronchoalveolar lavage of sheep lungs from slaughterhouse in the Metropolitan region of Fortaleza

Detalhes bibliográficos
Autor(a) principal: Martins, Gabrielle Rosemblit
Data de Publicação: 2014
Outros Autores: Teixeira, Maria Fátima da Silva, Barroso, Igor Ciríaco, Souza, Kelma Costa, Marinho, Rebeca Cavalcante, Junior, Rosivaldo Quirino Bezerra
Tipo de documento: Artigo
Idioma: por
Título da fonte: Brazilian Journal of Veterinary Medicine
Texto Completo: https://rbmv.org/BJVM/article/view/521
Resumo: ABSTRACT. Martins G.R., Teixeira M.F.S., Barroso I.C. Souza K.C., Marinho R.C. & Bezerra Junior R.Q. [Detection of Maedi visna virus, by Nested-PCR, in bronchoalveolar lavage of sheep lungs from slaughterhouse in the Metropolitan region of Fortaleza.] Detecção do vírus Maedi-visna, por Nested-PCR, no lavado bronco-alveolar de pulmões ovinos provenientes de abatedouro da região metropolitana de Fortaleza. Revista Brasileira de Medicina Veteriná- ria, 36(3):312-316, 2014. Programa de Pós-Graduação em Ciências Veterinárias, Universidade Estadual do Ceará, Av. Dr. Silas Munguba, 1700, Fortaleza, CE 60714-502, Brasil. E-mail: mfteixeira@hotmail.com Maedi-Visna is a disease multisystemic character caused by Maedi Visna Virus, a lentivirus belonging to the Retroviridae family, that preferentially infects cells of the mononuclear phagocyte system. The infected animal may show lesions in the lungs, joints, brain and mammary glands, singly or not, but usually the animal doesn’t shows symptoms, and should be resorted to laboratory tests for diagnosis. Seeking to demonstrate the presence of this virus in herds in this region, the aim of this study was to detect the presence of the Maedi-Visna Virus in bronchoalveolar lavage of sheep lungs from slaughterhouse in the Metropolitan Region of Fortaleza. For this purpose, samples of sheep lungs were collected from slaughterhouse in the region, did bronchoalveolar lavage and cultivation of the cells obtained. These samples were subjected to DNA extraction and amplification by Nested-PCR. The presence of proviral DNA was confirmed in six of the 58 samples analyzed, demonstrating the presence of this infection in properties in the Metropolitan Region of Fortaleza.
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spelling Detection of Maedi visna virus, by Nested-PCR, in bronchoalveolar lavage of sheep lungs from slaughterhouse in the Metropolitan region of FortalezaDetecção do vírus Maedi-visna, por Nested-PCR, no lavado bronco-alveolar de pulmões ovinos provenientes de abatedouro da região metropolitana de Fortaleza*Maedi-VisnapulmãoPCRMaedi-VisnaLungPCRABSTRACT. Martins G.R., Teixeira M.F.S., Barroso I.C. Souza K.C., Marinho R.C. & Bezerra Junior R.Q. [Detection of Maedi visna virus, by Nested-PCR, in bronchoalveolar lavage of sheep lungs from slaughterhouse in the Metropolitan region of Fortaleza.] Detecção do vírus Maedi-visna, por Nested-PCR, no lavado bronco-alveolar de pulmões ovinos provenientes de abatedouro da região metropolitana de Fortaleza. Revista Brasileira de Medicina Veteriná- ria, 36(3):312-316, 2014. Programa de Pós-Graduação em Ciências Veterinárias, Universidade Estadual do Ceará, Av. Dr. Silas Munguba, 1700, Fortaleza, CE 60714-502, Brasil. E-mail: mfteixeira@hotmail.com Maedi-Visna is a disease multisystemic character caused by Maedi Visna Virus, a lentivirus belonging to the Retroviridae family, that preferentially infects cells of the mononuclear phagocyte system. The infected animal may show lesions in the lungs, joints, brain and mammary glands, singly or not, but usually the animal doesn’t shows symptoms, and should be resorted to laboratory tests for diagnosis. Seeking to demonstrate the presence of this virus in herds in this region, the aim of this study was to detect the presence of the Maedi-Visna Virus in bronchoalveolar lavage of sheep lungs from slaughterhouse in the Metropolitan Region of Fortaleza. For this purpose, samples of sheep lungs were collected from slaughterhouse in the region, did bronchoalveolar lavage and cultivation of the cells obtained. These samples were subjected to DNA extraction and amplification by Nested-PCR. The presence of proviral DNA was confirmed in six of the 58 samples analyzed, demonstrating the presence of this infection in properties in the Metropolitan Region of Fortaleza.Maedi-Visna é uma doença de caráter multissistêmico causada pelo vírus Maedi-Visna, um lentivírus pertencente à família Retroviridae, que infecta preferencialmente as células do sistema fagocítico mononuclear. O animal infectado pode apresentar lesões nos pulmões, articulações, glândulas mamárias e cérebro, isoladamente ou não; porém, normalmente, o animal se apresenta assintomático, devendo-se recorrer a testes laboratoriais para seu diagnóstico. Buscando-se demonstrar a presença desse vírus nos rebanhos dessa região, o objetivo desse trabalho foi detectar a presença do vírus Maedi-Visna no lavado bronco-alveolar de pulmões ovinos provenientes de abatedouro da Região Metropolitana de Fortaleza. Para tanto, foram coletadas amostras de pulmões ovinos em abatedouro da região, realizado lavado bronco-alveolar e posterior cultivo das células obtidas. Essas amostras foram submetidas à extração de DNA e amplificação por Nested-PCR. A presença do DNA pró-viral foi confirmada em seis amostras das 58 analisadas, demonstrando a presença dessa infecção em propriedades da Região Metropolitana de Fortaleza.Sociedade de Medicina Veterinária do Estado do Rio de Janeiro.2014-09-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionpeer reviewedAvaliado pelos paresapplication/pdfhttps://rbmv.org/BJVM/article/view/521Brazilian Journal of Veterinary Medicine; Vol. 36 No. 3 (2014); 312-316Revista Brasileira de Medicina Veterinária; v. 36 n. 3 (2014); 312-3162527-21790100-2430reponame:Brazilian Journal of Veterinary Medicineinstname:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)instacron:SBMVporhttps://rbmv.org/BJVM/article/view/521/394Martins, Gabrielle RosemblitTeixeira, Maria Fátima da SilvaBarroso, Igor CiríacoSouza, Kelma CostaMarinho, Rebeca CavalcanteJunior, Rosivaldo Quirino Bezerrainfo:eu-repo/semantics/openAccess2020-12-23T17:32:32Zoai:ojs.rbmv.org:article/521Revistahttps://rbmv.org/BJVMONGhttps://rbmv.org/BJVM/oaicontato.rbmv@gmail.com2527-21790100-2430opendoar:2020-12-23T17:32:32Brazilian Journal of Veterinary Medicine - Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)false
dc.title.none.fl_str_mv Detection of Maedi visna virus, by Nested-PCR, in bronchoalveolar lavage of sheep lungs from slaughterhouse in the Metropolitan region of Fortaleza
Detecção do vírus Maedi-visna, por Nested-PCR, no lavado bronco-alveolar de pulmões ovinos provenientes de abatedouro da região metropolitana de Fortaleza*
title Detection of Maedi visna virus, by Nested-PCR, in bronchoalveolar lavage of sheep lungs from slaughterhouse in the Metropolitan region of Fortaleza
spellingShingle Detection of Maedi visna virus, by Nested-PCR, in bronchoalveolar lavage of sheep lungs from slaughterhouse in the Metropolitan region of Fortaleza
Martins, Gabrielle Rosemblit
Maedi-Visna
pulmão
PCR
Maedi-Visna
Lung
PCR
title_short Detection of Maedi visna virus, by Nested-PCR, in bronchoalveolar lavage of sheep lungs from slaughterhouse in the Metropolitan region of Fortaleza
title_full Detection of Maedi visna virus, by Nested-PCR, in bronchoalveolar lavage of sheep lungs from slaughterhouse in the Metropolitan region of Fortaleza
title_fullStr Detection of Maedi visna virus, by Nested-PCR, in bronchoalveolar lavage of sheep lungs from slaughterhouse in the Metropolitan region of Fortaleza
title_full_unstemmed Detection of Maedi visna virus, by Nested-PCR, in bronchoalveolar lavage of sheep lungs from slaughterhouse in the Metropolitan region of Fortaleza
title_sort Detection of Maedi visna virus, by Nested-PCR, in bronchoalveolar lavage of sheep lungs from slaughterhouse in the Metropolitan region of Fortaleza
author Martins, Gabrielle Rosemblit
author_facet Martins, Gabrielle Rosemblit
Teixeira, Maria Fátima da Silva
Barroso, Igor Ciríaco
Souza, Kelma Costa
Marinho, Rebeca Cavalcante
Junior, Rosivaldo Quirino Bezerra
author_role author
author2 Teixeira, Maria Fátima da Silva
Barroso, Igor Ciríaco
Souza, Kelma Costa
Marinho, Rebeca Cavalcante
Junior, Rosivaldo Quirino Bezerra
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Martins, Gabrielle Rosemblit
Teixeira, Maria Fátima da Silva
Barroso, Igor Ciríaco
Souza, Kelma Costa
Marinho, Rebeca Cavalcante
Junior, Rosivaldo Quirino Bezerra
dc.subject.por.fl_str_mv Maedi-Visna
pulmão
PCR
Maedi-Visna
Lung
PCR
topic Maedi-Visna
pulmão
PCR
Maedi-Visna
Lung
PCR
description ABSTRACT. Martins G.R., Teixeira M.F.S., Barroso I.C. Souza K.C., Marinho R.C. & Bezerra Junior R.Q. [Detection of Maedi visna virus, by Nested-PCR, in bronchoalveolar lavage of sheep lungs from slaughterhouse in the Metropolitan region of Fortaleza.] Detecção do vírus Maedi-visna, por Nested-PCR, no lavado bronco-alveolar de pulmões ovinos provenientes de abatedouro da região metropolitana de Fortaleza. Revista Brasileira de Medicina Veteriná- ria, 36(3):312-316, 2014. Programa de Pós-Graduação em Ciências Veterinárias, Universidade Estadual do Ceará, Av. Dr. Silas Munguba, 1700, Fortaleza, CE 60714-502, Brasil. E-mail: mfteixeira@hotmail.com Maedi-Visna is a disease multisystemic character caused by Maedi Visna Virus, a lentivirus belonging to the Retroviridae family, that preferentially infects cells of the mononuclear phagocyte system. The infected animal may show lesions in the lungs, joints, brain and mammary glands, singly or not, but usually the animal doesn’t shows symptoms, and should be resorted to laboratory tests for diagnosis. Seeking to demonstrate the presence of this virus in herds in this region, the aim of this study was to detect the presence of the Maedi-Visna Virus in bronchoalveolar lavage of sheep lungs from slaughterhouse in the Metropolitan Region of Fortaleza. For this purpose, samples of sheep lungs were collected from slaughterhouse in the region, did bronchoalveolar lavage and cultivation of the cells obtained. These samples were subjected to DNA extraction and amplification by Nested-PCR. The presence of proviral DNA was confirmed in six of the 58 samples analyzed, demonstrating the presence of this infection in properties in the Metropolitan Region of Fortaleza.
publishDate 2014
dc.date.none.fl_str_mv 2014-09-10
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
peer reviewed
Avaliado pelos pares
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rbmv.org/BJVM/article/view/521
url https://rbmv.org/BJVM/article/view/521
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://rbmv.org/BJVM/article/view/521/394
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Sociedade de Medicina Veterinária do Estado do Rio de Janeiro.
publisher.none.fl_str_mv Sociedade de Medicina Veterinária do Estado do Rio de Janeiro.
dc.source.none.fl_str_mv Brazilian Journal of Veterinary Medicine; Vol. 36 No. 3 (2014); 312-316
Revista Brasileira de Medicina Veterinária; v. 36 n. 3 (2014); 312-316
2527-2179
0100-2430
reponame:Brazilian Journal of Veterinary Medicine
instname:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)
instacron:SBMV
instname_str Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)
instacron_str SBMV
institution SBMV
reponame_str Brazilian Journal of Veterinary Medicine
collection Brazilian Journal of Veterinary Medicine
repository.name.fl_str_mv Brazilian Journal of Veterinary Medicine - Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)
repository.mail.fl_str_mv contato.rbmv@gmail.com
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