Autohemotherapy increases phagocytic activity of neutrophils and promotes cytokine production by lymphocytes in horses

Detalhes bibliográficos
Autor(a) principal: Lopes, Paula Rodrigues
Data de Publicação: 2021
Outros Autores: Batista, Mauricio Azevedo, Soares, Rodrigo Pedro, de Oliveira, Jéssica Guerra, Leme, Fabiola de Oliveira Paes, Martins-Filho, Olindo Assis, Maranhão, Renata de Pino Albuquerque, Wenceslau, Raphael Rocha, Palhares, Maristela Silveira, Araújo, Marcio Sobreira Silva
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Veterinary Medicine
Texto Completo: https://rbmv.org/BJVM/article/view/1187
Resumo: Autohemotherapy (AHT) is a procedure in which blood is collected by venipuncture, followed by immediate reinjection to activate an animal’s immune system. In this preliminary study, we evaluated the effect of AHT on clinical, hematological and biochemical parameters and immune responses in horses. Eight healthy animals (four males and four females) were clinically evaluated and subjected to AHT (three applications at a weekly interval). Blood samples were collected before AHT (T0) and 3 d after each procedure (T1, T2, and T3). AHT did not affect most clinical, biochemical, and hematological parameters in the test animals. However, flow cytometry detected an increase in T lymphocytes (CD3+) and cytokine production (IFN-γ, IL-10, IL-4, and IL-6). In addition, AHT stimulated phagocytosis by neutrophils at T3. Although preliminary, our results suggest that AHT is a promising procedure for equine management, as AHT was found to stimulate the horses’ immune responses without affecting their general status.
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spelling Autohemotherapy increases phagocytic activity of neutrophils and promotes cytokine production by lymphocytes in horsesAuto-hemoterapia aumenta a atividade fagocítica dos neutrófilos e promove a produção de citocinas pelos linfócitos em cavalosautohemoterapia, cavalo, citocinas, fagocitose.autohemotherapy, horse, cytokines, phagocytosis.Autohemotherapy (AHT) is a procedure in which blood is collected by venipuncture, followed by immediate reinjection to activate an animal’s immune system. In this preliminary study, we evaluated the effect of AHT on clinical, hematological and biochemical parameters and immune responses in horses. Eight healthy animals (four males and four females) were clinically evaluated and subjected to AHT (three applications at a weekly interval). Blood samples were collected before AHT (T0) and 3 d after each procedure (T1, T2, and T3). AHT did not affect most clinical, biochemical, and hematological parameters in the test animals. However, flow cytometry detected an increase in T lymphocytes (CD3+) and cytokine production (IFN-γ, IL-10, IL-4, and IL-6). In addition, AHT stimulated phagocytosis by neutrophils at T3. Although preliminary, our results suggest that AHT is a promising procedure for equine management, as AHT was found to stimulate the horses’ immune responses without affecting their general status.A auto-hemoterapia (AHT) é um procedimento em que o sangue é coletado por punção venosa, seguida de imediata reinjeção, com o objetivo de ativar o sistema imunológico do animal. O objetivo deste estudo preliminar foi avaliar o impacto do AHT nos parâmetros clínicos, hematológicos, bioquímicos e de respostas imunológicas em cavalos. Oito animais saudáveis (4 machos e 4 fêmeas) foram avaliados clinicamente e submetidos a AHT (3 aplicações, com intervalo de uma semana). O sangue foi coletado antes do AHT (T0) e 3 dias após cada procedimento (T1, T2 e T3). Em geral, o AHT não afetou consideravelmente os parâmetros clínicos, bioquímicos e hematológicos dos cavalos. No entanto, as análises de citometria de fluxo detectaram um pico de linfócitos T (CD3+) e produção de citocinas (IFN-γ, IL-10, IL-4 e IL-6) em T1 e T2. Além disso, o AHT estimulou a atividade de fagocitose nos neutrófilos, especialmente durante o T3. Embora preliminares, nossos resultados sugerem que a AHT pode ser procedimento promissor para o manejo de equinos. A AHT estimula a resposta imune sem afetar o estado geral do animal.Sociedade de Medicina Veterinária do Estado do Rio de Janeiro.2021-06-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionpeer reviewedAvaliado pelos paresapplication/pdftext/xmlhttps://rbmv.org/BJVM/article/view/118710.29374/2527-2179.bjvm000821Brazilian Journal of Veterinary Medicine; Vol. 43 No. 1 (2021); e000821Revista Brasileira de Medicina Veterinária; v. 43 n. 1 (2021); e0008212527-21790100-2430reponame:Brazilian Journal of Veterinary Medicineinstname:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)instacron:SBMVenghttps://rbmv.org/BJVM/article/view/1187/1091https://rbmv.org/BJVM/article/view/1187/1192Copyright (c) 2021 Paula Rodrigues Lopes, Mauricio Azevedo Batista, Rodrigo Pedro Soares, Jéssica Guerra de Oliveira, Fabiola de Oliveira Paes Leme, Olindo Assis Martins-Filho, Renata de Pino Albuquerque Maranhão, Raphael Rocha Wenceslau, Maristela Silveira Palhares, Marcio Sobreira Silva Araújohttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessLopes, Paula RodriguesBatista, Mauricio AzevedoSoares, Rodrigo Pedrode Oliveira, Jéssica GuerraLeme, Fabiola de Oliveira PaesMartins-Filho, Olindo AssisMaranhão, Renata de Pino AlbuquerqueWenceslau, Raphael RochaPalhares, Maristela SilveiraAraújo, Marcio Sobreira Silva2022-06-26T17:54:01Zoai:ojs.rbmv.org:article/1187Revistahttps://rbmv.org/BJVMONGhttps://rbmv.org/BJVM/oaicontato.rbmv@gmail.com2527-21790100-2430opendoar:2022-06-26T17:54:01Brazilian Journal of Veterinary Medicine - Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)false
dc.title.none.fl_str_mv Autohemotherapy increases phagocytic activity of neutrophils and promotes cytokine production by lymphocytes in horses
Auto-hemoterapia aumenta a atividade fagocítica dos neutrófilos e promove a produção de citocinas pelos linfócitos em cavalos
title Autohemotherapy increases phagocytic activity of neutrophils and promotes cytokine production by lymphocytes in horses
spellingShingle Autohemotherapy increases phagocytic activity of neutrophils and promotes cytokine production by lymphocytes in horses
Lopes, Paula Rodrigues
autohemoterapia, cavalo, citocinas, fagocitose.
autohemotherapy, horse, cytokines, phagocytosis.
title_short Autohemotherapy increases phagocytic activity of neutrophils and promotes cytokine production by lymphocytes in horses
title_full Autohemotherapy increases phagocytic activity of neutrophils and promotes cytokine production by lymphocytes in horses
title_fullStr Autohemotherapy increases phagocytic activity of neutrophils and promotes cytokine production by lymphocytes in horses
title_full_unstemmed Autohemotherapy increases phagocytic activity of neutrophils and promotes cytokine production by lymphocytes in horses
title_sort Autohemotherapy increases phagocytic activity of neutrophils and promotes cytokine production by lymphocytes in horses
author Lopes, Paula Rodrigues
author_facet Lopes, Paula Rodrigues
Batista, Mauricio Azevedo
Soares, Rodrigo Pedro
de Oliveira, Jéssica Guerra
Leme, Fabiola de Oliveira Paes
Martins-Filho, Olindo Assis
Maranhão, Renata de Pino Albuquerque
Wenceslau, Raphael Rocha
Palhares, Maristela Silveira
Araújo, Marcio Sobreira Silva
author_role author
author2 Batista, Mauricio Azevedo
Soares, Rodrigo Pedro
de Oliveira, Jéssica Guerra
Leme, Fabiola de Oliveira Paes
Martins-Filho, Olindo Assis
Maranhão, Renata de Pino Albuquerque
Wenceslau, Raphael Rocha
Palhares, Maristela Silveira
Araújo, Marcio Sobreira Silva
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Lopes, Paula Rodrigues
Batista, Mauricio Azevedo
Soares, Rodrigo Pedro
de Oliveira, Jéssica Guerra
Leme, Fabiola de Oliveira Paes
Martins-Filho, Olindo Assis
Maranhão, Renata de Pino Albuquerque
Wenceslau, Raphael Rocha
Palhares, Maristela Silveira
Araújo, Marcio Sobreira Silva
dc.subject.por.fl_str_mv autohemoterapia, cavalo, citocinas, fagocitose.
autohemotherapy, horse, cytokines, phagocytosis.
topic autohemoterapia, cavalo, citocinas, fagocitose.
autohemotherapy, horse, cytokines, phagocytosis.
description Autohemotherapy (AHT) is a procedure in which blood is collected by venipuncture, followed by immediate reinjection to activate an animal’s immune system. In this preliminary study, we evaluated the effect of AHT on clinical, hematological and biochemical parameters and immune responses in horses. Eight healthy animals (four males and four females) were clinically evaluated and subjected to AHT (three applications at a weekly interval). Blood samples were collected before AHT (T0) and 3 d after each procedure (T1, T2, and T3). AHT did not affect most clinical, biochemical, and hematological parameters in the test animals. However, flow cytometry detected an increase in T lymphocytes (CD3+) and cytokine production (IFN-γ, IL-10, IL-4, and IL-6). In addition, AHT stimulated phagocytosis by neutrophils at T3. Although preliminary, our results suggest that AHT is a promising procedure for equine management, as AHT was found to stimulate the horses’ immune responses without affecting their general status.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-14
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
peer reviewed
Avaliado pelos pares
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rbmv.org/BJVM/article/view/1187
10.29374/2527-2179.bjvm000821
url https://rbmv.org/BJVM/article/view/1187
identifier_str_mv 10.29374/2527-2179.bjvm000821
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://rbmv.org/BJVM/article/view/1187/1091
https://rbmv.org/BJVM/article/view/1187/1192
dc.rights.driver.fl_str_mv http://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
text/xml
dc.publisher.none.fl_str_mv Sociedade de Medicina Veterinária do Estado do Rio de Janeiro.
publisher.none.fl_str_mv Sociedade de Medicina Veterinária do Estado do Rio de Janeiro.
dc.source.none.fl_str_mv Brazilian Journal of Veterinary Medicine; Vol. 43 No. 1 (2021); e000821
Revista Brasileira de Medicina Veterinária; v. 43 n. 1 (2021); e000821
2527-2179
0100-2430
reponame:Brazilian Journal of Veterinary Medicine
instname:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)
instacron:SBMV
instname_str Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)
instacron_str SBMV
institution SBMV
reponame_str Brazilian Journal of Veterinary Medicine
collection Brazilian Journal of Veterinary Medicine
repository.name.fl_str_mv Brazilian Journal of Veterinary Medicine - Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)
repository.mail.fl_str_mv contato.rbmv@gmail.com
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