Autohemotherapy increases phagocytic activity of neutrophils and promotes cytokine production by lymphocytes in horses
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Veterinary Medicine |
Texto Completo: | https://rbmv.org/BJVM/article/view/1187 |
Resumo: | Autohemotherapy (AHT) is a procedure in which blood is collected by venipuncture, followed by immediate reinjection to activate an animal’s immune system. In this preliminary study, we evaluated the effect of AHT on clinical, hematological and biochemical parameters and immune responses in horses. Eight healthy animals (four males and four females) were clinically evaluated and subjected to AHT (three applications at a weekly interval). Blood samples were collected before AHT (T0) and 3 d after each procedure (T1, T2, and T3). AHT did not affect most clinical, biochemical, and hematological parameters in the test animals. However, flow cytometry detected an increase in T lymphocytes (CD3+) and cytokine production (IFN-γ, IL-10, IL-4, and IL-6). In addition, AHT stimulated phagocytosis by neutrophils at T3. Although preliminary, our results suggest that AHT is a promising procedure for equine management, as AHT was found to stimulate the horses’ immune responses without affecting their general status. |
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Brazilian Journal of Veterinary Medicine |
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Autohemotherapy increases phagocytic activity of neutrophils and promotes cytokine production by lymphocytes in horsesAuto-hemoterapia aumenta a atividade fagocítica dos neutrófilos e promove a produção de citocinas pelos linfócitos em cavalosautohemoterapia, cavalo, citocinas, fagocitose.autohemotherapy, horse, cytokines, phagocytosis.Autohemotherapy (AHT) is a procedure in which blood is collected by venipuncture, followed by immediate reinjection to activate an animal’s immune system. In this preliminary study, we evaluated the effect of AHT on clinical, hematological and biochemical parameters and immune responses in horses. Eight healthy animals (four males and four females) were clinically evaluated and subjected to AHT (three applications at a weekly interval). Blood samples were collected before AHT (T0) and 3 d after each procedure (T1, T2, and T3). AHT did not affect most clinical, biochemical, and hematological parameters in the test animals. However, flow cytometry detected an increase in T lymphocytes (CD3+) and cytokine production (IFN-γ, IL-10, IL-4, and IL-6). In addition, AHT stimulated phagocytosis by neutrophils at T3. Although preliminary, our results suggest that AHT is a promising procedure for equine management, as AHT was found to stimulate the horses’ immune responses without affecting their general status.A auto-hemoterapia (AHT) é um procedimento em que o sangue é coletado por punção venosa, seguida de imediata reinjeção, com o objetivo de ativar o sistema imunológico do animal. O objetivo deste estudo preliminar foi avaliar o impacto do AHT nos parâmetros clínicos, hematológicos, bioquímicos e de respostas imunológicas em cavalos. Oito animais saudáveis (4 machos e 4 fêmeas) foram avaliados clinicamente e submetidos a AHT (3 aplicações, com intervalo de uma semana). O sangue foi coletado antes do AHT (T0) e 3 dias após cada procedimento (T1, T2 e T3). Em geral, o AHT não afetou consideravelmente os parâmetros clínicos, bioquímicos e hematológicos dos cavalos. No entanto, as análises de citometria de fluxo detectaram um pico de linfócitos T (CD3+) e produção de citocinas (IFN-γ, IL-10, IL-4 e IL-6) em T1 e T2. Além disso, o AHT estimulou a atividade de fagocitose nos neutrófilos, especialmente durante o T3. Embora preliminares, nossos resultados sugerem que a AHT pode ser procedimento promissor para o manejo de equinos. A AHT estimula a resposta imune sem afetar o estado geral do animal.Sociedade de Medicina Veterinária do Estado do Rio de Janeiro.2021-06-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionpeer reviewedAvaliado pelos paresapplication/pdftext/xmlhttps://rbmv.org/BJVM/article/view/118710.29374/2527-2179.bjvm000821Brazilian Journal of Veterinary Medicine; Vol. 43 No. 1 (2021); e000821Revista Brasileira de Medicina Veterinária; v. 43 n. 1 (2021); e0008212527-21790100-2430reponame:Brazilian Journal of Veterinary Medicineinstname:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)instacron:SBMVenghttps://rbmv.org/BJVM/article/view/1187/1091https://rbmv.org/BJVM/article/view/1187/1192Copyright (c) 2021 Paula Rodrigues Lopes, Mauricio Azevedo Batista, Rodrigo Pedro Soares, Jéssica Guerra de Oliveira, Fabiola de Oliveira Paes Leme, Olindo Assis Martins-Filho, Renata de Pino Albuquerque Maranhão, Raphael Rocha Wenceslau, Maristela Silveira Palhares, Marcio Sobreira Silva Araújohttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessLopes, Paula RodriguesBatista, Mauricio AzevedoSoares, Rodrigo Pedrode Oliveira, Jéssica GuerraLeme, Fabiola de Oliveira PaesMartins-Filho, Olindo AssisMaranhão, Renata de Pino AlbuquerqueWenceslau, Raphael RochaPalhares, Maristela SilveiraAraújo, Marcio Sobreira Silva2022-06-26T17:54:01Zoai:ojs.rbmv.org:article/1187Revistahttps://rbmv.org/BJVMONGhttps://rbmv.org/BJVM/oaicontato.rbmv@gmail.com2527-21790100-2430opendoar:2022-06-26T17:54:01Brazilian Journal of Veterinary Medicine - Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)false |
dc.title.none.fl_str_mv |
Autohemotherapy increases phagocytic activity of neutrophils and promotes cytokine production by lymphocytes in horses Auto-hemoterapia aumenta a atividade fagocítica dos neutrófilos e promove a produção de citocinas pelos linfócitos em cavalos |
title |
Autohemotherapy increases phagocytic activity of neutrophils and promotes cytokine production by lymphocytes in horses |
spellingShingle |
Autohemotherapy increases phagocytic activity of neutrophils and promotes cytokine production by lymphocytes in horses Lopes, Paula Rodrigues autohemoterapia, cavalo, citocinas, fagocitose. autohemotherapy, horse, cytokines, phagocytosis. |
title_short |
Autohemotherapy increases phagocytic activity of neutrophils and promotes cytokine production by lymphocytes in horses |
title_full |
Autohemotherapy increases phagocytic activity of neutrophils and promotes cytokine production by lymphocytes in horses |
title_fullStr |
Autohemotherapy increases phagocytic activity of neutrophils and promotes cytokine production by lymphocytes in horses |
title_full_unstemmed |
Autohemotherapy increases phagocytic activity of neutrophils and promotes cytokine production by lymphocytes in horses |
title_sort |
Autohemotherapy increases phagocytic activity of neutrophils and promotes cytokine production by lymphocytes in horses |
author |
Lopes, Paula Rodrigues |
author_facet |
Lopes, Paula Rodrigues Batista, Mauricio Azevedo Soares, Rodrigo Pedro de Oliveira, Jéssica Guerra Leme, Fabiola de Oliveira Paes Martins-Filho, Olindo Assis Maranhão, Renata de Pino Albuquerque Wenceslau, Raphael Rocha Palhares, Maristela Silveira Araújo, Marcio Sobreira Silva |
author_role |
author |
author2 |
Batista, Mauricio Azevedo Soares, Rodrigo Pedro de Oliveira, Jéssica Guerra Leme, Fabiola de Oliveira Paes Martins-Filho, Olindo Assis Maranhão, Renata de Pino Albuquerque Wenceslau, Raphael Rocha Palhares, Maristela Silveira Araújo, Marcio Sobreira Silva |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Lopes, Paula Rodrigues Batista, Mauricio Azevedo Soares, Rodrigo Pedro de Oliveira, Jéssica Guerra Leme, Fabiola de Oliveira Paes Martins-Filho, Olindo Assis Maranhão, Renata de Pino Albuquerque Wenceslau, Raphael Rocha Palhares, Maristela Silveira Araújo, Marcio Sobreira Silva |
dc.subject.por.fl_str_mv |
autohemoterapia, cavalo, citocinas, fagocitose. autohemotherapy, horse, cytokines, phagocytosis. |
topic |
autohemoterapia, cavalo, citocinas, fagocitose. autohemotherapy, horse, cytokines, phagocytosis. |
description |
Autohemotherapy (AHT) is a procedure in which blood is collected by venipuncture, followed by immediate reinjection to activate an animal’s immune system. In this preliminary study, we evaluated the effect of AHT on clinical, hematological and biochemical parameters and immune responses in horses. Eight healthy animals (four males and four females) were clinically evaluated and subjected to AHT (three applications at a weekly interval). Blood samples were collected before AHT (T0) and 3 d after each procedure (T1, T2, and T3). AHT did not affect most clinical, biochemical, and hematological parameters in the test animals. However, flow cytometry detected an increase in T lymphocytes (CD3+) and cytokine production (IFN-γ, IL-10, IL-4, and IL-6). In addition, AHT stimulated phagocytosis by neutrophils at T3. Although preliminary, our results suggest that AHT is a promising procedure for equine management, as AHT was found to stimulate the horses’ immune responses without affecting their general status. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-14 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion peer reviewed Avaliado pelos pares |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rbmv.org/BJVM/article/view/1187 10.29374/2527-2179.bjvm000821 |
url |
https://rbmv.org/BJVM/article/view/1187 |
identifier_str_mv |
10.29374/2527-2179.bjvm000821 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rbmv.org/BJVM/article/view/1187/1091 https://rbmv.org/BJVM/article/view/1187/1192 |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf text/xml |
dc.publisher.none.fl_str_mv |
Sociedade de Medicina Veterinária do Estado do Rio de Janeiro. |
publisher.none.fl_str_mv |
Sociedade de Medicina Veterinária do Estado do Rio de Janeiro. |
dc.source.none.fl_str_mv |
Brazilian Journal of Veterinary Medicine; Vol. 43 No. 1 (2021); e000821 Revista Brasileira de Medicina Veterinária; v. 43 n. 1 (2021); e000821 2527-2179 0100-2430 reponame:Brazilian Journal of Veterinary Medicine instname:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ) instacron:SBMV |
instname_str |
Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ) |
instacron_str |
SBMV |
institution |
SBMV |
reponame_str |
Brazilian Journal of Veterinary Medicine |
collection |
Brazilian Journal of Veterinary Medicine |
repository.name.fl_str_mv |
Brazilian Journal of Veterinary Medicine - Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ) |
repository.mail.fl_str_mv |
contato.rbmv@gmail.com |
_version_ |
1798313111522902016 |