Bacterial isolation and evaluation of antisepsis protocols of the operative field of bitches submitted to ovariohysterectomy

• Autor(a) principal: Cândido Trajano, Sabrina
• Data de Publicação: 2021
• Outros Autores: Bezerra Aragão, Breno, de Araujo Penaforte Junior, Mauro, Domingos de Melo, Kleyton, da Silva Ferreira, Maria Sheila, Baltazar de Oliveira, Júnior Mário, Aparecido Mota, Rinaldo, de Sousa Aleixo, Grazielle Anahy
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Brazilian Journal of Veterinary Medicine
• Texto Completo: https://rbmv.org/BJVM/article/view/1059
• topic: protocolo de antissepsia, Cannis familiaris, cirurgia eletiva, sítio cirúrgico; antisepsis protocol, Cannis familiaris, elective surgery, surgical site.

 This study evaluated the efficiency of five antisepsis protocols performed in the operative field of bitches due to the importance of the prevention of surgical infections in veterinary medicine. Thirty female bitches submitted to elective ovariohysterectomy (OVH) were used, separated into groups of eight animals. In group I, 70% alcohol and 2% chlorhexidine gluconate (CHG) were administered in group II, alcoholic  CHG 0.5%, in group III polyvinylpyrrolidone (PVPI) 10% alcohol, group IV alcohol 70% and alcoholic PVPI 10 %, and in group V, alcohol, PVPI 10% and CHG degermante 2%. Samples were collected with sterile swabs prior to antisepsis and three minutes after each product was used and seeded in specific medium for colony-forming unit (CFU) counts. The identification of the isolates was performed according to the morphological, dyeing and biochemical characteristics. Bacillus spp., Staphylococcus spp., Klebsiella pnemoniae, Klebsiella oxytoca, Shigella sonnei, Shigella spp., Enterobacter aerogenes, Enterobacter spp., Salmonella spp. and Escherichia coli. The results obtained allow us to conclude that the protocols which CHG 0.5% alcohol and CHG 2% degermante were the most efficient in antisepsis, reducing 100% of the CFU of the skin and presented better residual power until the end of the surgical procedure.