Evaluation of different parasitological techniques in the diagnosis of canine helminthiasis
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Brazilian Journal of Veterinary Medicine |
Texto Completo: | https://rbmv.org/BJVM/article/view/463 |
Resumo: | ABSTRACT. Novaes M.T. & Martins I.V.F. [Evaluation of different parasitological techniques in the diagnosis of canine helminthiasis.] Avaliação de diferentes técnicas parasitológicas no diagnóstico de helmintoses caninas. Revista Brasileira de Medicina Veterinária, 37(Supl.1):71-76, 2015. Laboratório de Parasitologia, Hospital Veterinário, Universidade Federal do Espírito Santo, Rua Alto Universitário, s/n, Guararema, Alegre, ES 29500-000, Brasil. E-mail: marcelle_temporim@hotmail.com Were collected 100 fecal samples from dogs from August 2014 to April 2015 from the Zoonosis Control Center (CCZ) of Cachoeiro de Itapemirim-ES. These were sent to the laboratory of Parasitology of the Veterinary Hospital of UFES, and analyzed in duplicate by the techniques of Kato-Katz, Hoffman, TF-Test Modified/Dog and Willis in order to evaluate the technique that present higher sensitivity, convenience and lower expenses with materials for parasitological diagnosis. Statistical analysis was performed using Fisher’s exact test and kappa the 5% significance level. Together they showed up 68 positive samples, 31, 34, 26 and 62 for technical Kato-Katz, Hoffman, TF-Test and Willis respectively. The results showed that the use of Willis technique should be adopted as the standard for egg Ancylostoma spp., since the techniques Kato-Katz, Hoffman and TF-Test showed a low sensitivity for diagnosis of this parasite, and technic of Hoffman should be used as standard for Toxocara spp. In relation to convenience and lower expenses, Willis technique, should be preferred, because the runtime and the microscope slide reading is fast, requiring only the use of a supersaturated solution. |
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Evaluation of different parasitological techniques in the diagnosis of canine helminthiasisAvaliação de diferentes técnicas parasitológicas no diagnóstico de helmintoses caninasHelmintosestécnicas laboratoriaissensibilidadeHelminthslaboratory techniquessensibilityABSTRACT. Novaes M.T. & Martins I.V.F. [Evaluation of different parasitological techniques in the diagnosis of canine helminthiasis.] Avaliação de diferentes técnicas parasitológicas no diagnóstico de helmintoses caninas. Revista Brasileira de Medicina Veterinária, 37(Supl.1):71-76, 2015. Laboratório de Parasitologia, Hospital Veterinário, Universidade Federal do Espírito Santo, Rua Alto Universitário, s/n, Guararema, Alegre, ES 29500-000, Brasil. E-mail: marcelle_temporim@hotmail.com Were collected 100 fecal samples from dogs from August 2014 to April 2015 from the Zoonosis Control Center (CCZ) of Cachoeiro de Itapemirim-ES. These were sent to the laboratory of Parasitology of the Veterinary Hospital of UFES, and analyzed in duplicate by the techniques of Kato-Katz, Hoffman, TF-Test Modified/Dog and Willis in order to evaluate the technique that present higher sensitivity, convenience and lower expenses with materials for parasitological diagnosis. Statistical analysis was performed using Fisher’s exact test and kappa the 5% significance level. Together they showed up 68 positive samples, 31, 34, 26 and 62 for technical Kato-Katz, Hoffman, TF-Test and Willis respectively. The results showed that the use of Willis technique should be adopted as the standard for egg Ancylostoma spp., since the techniques Kato-Katz, Hoffman and TF-Test showed a low sensitivity for diagnosis of this parasite, and technic of Hoffman should be used as standard for Toxocara spp. In relation to convenience and lower expenses, Willis technique, should be preferred, because the runtime and the microscope slide reading is fast, requiring only the use of a supersaturated solution.Foram coletadas 100 amostras fecais de cães no período de agosto de 2014 a abril de 2015 provenientes do Centro de Controle de Zoonose (CCZ) de Cachoeiro de Itapemirim-ES. Estas foram encaminhadas ao laboratório de parasitologia do Hospital Veterinário da UFES, e analisadas em duplicata pelas técnicas de Kato-Katz, Hoffman, TF-Test Modified/ Dog e Willis com a finalidade de avaliar a técnica que apresentaria maior sensibilidade, praticidade e menor gasto com materiais para o diagnóstico parasitológico. Para a análise estatística foi utilizado o teste Exato de Fisher e índice kappa a 5% de significância. Em conjunto apresentaram-se 68 amostras positivas, sendo 31, 34, 26 e 62 para as técnicas de Kato-Katz, Hoffman, TF-Test e Willis respectivamente. Os resultados demonstraram que a utilização da técnica de Willis deve ser adotada como padrão para ovos de Ancylostoma spp., uma vez que as técnicas de Kato- -Katz, Hoffman e TF-Test demonstraram uma sensibilidade baixa para o diagnóstico deste parasito, e a técnica de Hoffman deve ser utilizada como padrão para ovos de Toxocara spp. Com relação a praticidade e menor gasto com materiais, a técnica de Willis deve ser preferida, pois o tempo de execução e leitura da lâmina no microscópio é rápido, requerendo apenas o uso de uma solução supersaturada.Sociedade de Medicina Veterinária do Estado do Rio de Janeiro.2015-12-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionpeer reviewedAvaliado pelos paresapplication/pdfhttps://rbmv.org/BJVM/article/view/463Brazilian Journal of Veterinary Medicine; Vol. 37 No. Supl.1 (2015); 71-76Revista Brasileira de Medicina Veterinária; v. 37 n. Supl.1 (2015); 71-762527-21790100-2430reponame:Brazilian Journal of Veterinary Medicineinstname:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)instacron:SBMVporhttps://rbmv.org/BJVM/article/view/463/350Novaes, Marcelle TemporimMartins, Isabella Vilhena Freireinfo:eu-repo/semantics/openAccess2020-12-23T17:33:50Zoai:ojs.rbmv.org:article/463Revistahttps://rbmv.org/BJVMONGhttps://rbmv.org/BJVM/oaicontato.rbmv@gmail.com2527-21790100-2430opendoar:2020-12-23T17:33:50Brazilian Journal of Veterinary Medicine - Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)false |
dc.title.none.fl_str_mv |
Evaluation of different parasitological techniques in the diagnosis of canine helminthiasis Avaliação de diferentes técnicas parasitológicas no diagnóstico de helmintoses caninas |
title |
Evaluation of different parasitological techniques in the diagnosis of canine helminthiasis |
spellingShingle |
Evaluation of different parasitological techniques in the diagnosis of canine helminthiasis Novaes, Marcelle Temporim Helmintoses técnicas laboratoriais sensibilidade Helminths laboratory techniques sensibility |
title_short |
Evaluation of different parasitological techniques in the diagnosis of canine helminthiasis |
title_full |
Evaluation of different parasitological techniques in the diagnosis of canine helminthiasis |
title_fullStr |
Evaluation of different parasitological techniques in the diagnosis of canine helminthiasis |
title_full_unstemmed |
Evaluation of different parasitological techniques in the diagnosis of canine helminthiasis |
title_sort |
Evaluation of different parasitological techniques in the diagnosis of canine helminthiasis |
author |
Novaes, Marcelle Temporim |
author_facet |
Novaes, Marcelle Temporim Martins, Isabella Vilhena Freire |
author_role |
author |
author2 |
Martins, Isabella Vilhena Freire |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Novaes, Marcelle Temporim Martins, Isabella Vilhena Freire |
dc.subject.por.fl_str_mv |
Helmintoses técnicas laboratoriais sensibilidade Helminths laboratory techniques sensibility |
topic |
Helmintoses técnicas laboratoriais sensibilidade Helminths laboratory techniques sensibility |
description |
ABSTRACT. Novaes M.T. & Martins I.V.F. [Evaluation of different parasitological techniques in the diagnosis of canine helminthiasis.] Avaliação de diferentes técnicas parasitológicas no diagnóstico de helmintoses caninas. Revista Brasileira de Medicina Veterinária, 37(Supl.1):71-76, 2015. Laboratório de Parasitologia, Hospital Veterinário, Universidade Federal do Espírito Santo, Rua Alto Universitário, s/n, Guararema, Alegre, ES 29500-000, Brasil. E-mail: marcelle_temporim@hotmail.com Were collected 100 fecal samples from dogs from August 2014 to April 2015 from the Zoonosis Control Center (CCZ) of Cachoeiro de Itapemirim-ES. These were sent to the laboratory of Parasitology of the Veterinary Hospital of UFES, and analyzed in duplicate by the techniques of Kato-Katz, Hoffman, TF-Test Modified/Dog and Willis in order to evaluate the technique that present higher sensitivity, convenience and lower expenses with materials for parasitological diagnosis. Statistical analysis was performed using Fisher’s exact test and kappa the 5% significance level. Together they showed up 68 positive samples, 31, 34, 26 and 62 for technical Kato-Katz, Hoffman, TF-Test and Willis respectively. The results showed that the use of Willis technique should be adopted as the standard for egg Ancylostoma spp., since the techniques Kato-Katz, Hoffman and TF-Test showed a low sensitivity for diagnosis of this parasite, and technic of Hoffman should be used as standard for Toxocara spp. In relation to convenience and lower expenses, Willis technique, should be preferred, because the runtime and the microscope slide reading is fast, requiring only the use of a supersaturated solution. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-12-14 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion peer reviewed Avaliado pelos pares |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rbmv.org/BJVM/article/view/463 |
url |
https://rbmv.org/BJVM/article/view/463 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://rbmv.org/BJVM/article/view/463/350 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade de Medicina Veterinária do Estado do Rio de Janeiro. |
publisher.none.fl_str_mv |
Sociedade de Medicina Veterinária do Estado do Rio de Janeiro. |
dc.source.none.fl_str_mv |
Brazilian Journal of Veterinary Medicine; Vol. 37 No. Supl.1 (2015); 71-76 Revista Brasileira de Medicina Veterinária; v. 37 n. Supl.1 (2015); 71-76 2527-2179 0100-2430 reponame:Brazilian Journal of Veterinary Medicine instname:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ) instacron:SBMV |
instname_str |
Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ) |
instacron_str |
SBMV |
institution |
SBMV |
reponame_str |
Brazilian Journal of Veterinary Medicine |
collection |
Brazilian Journal of Veterinary Medicine |
repository.name.fl_str_mv |
Brazilian Journal of Veterinary Medicine - Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ) |
repository.mail.fl_str_mv |
contato.rbmv@gmail.com |
_version_ |
1798313108096155648 |