Escherichia coli in broiler chickens with airsacculitis

Detalhes bibliográficos
Autor(a) principal: Machado, Leandro S.
Data de Publicação: 2014
Outros Autores: do Nascimento, Elmiro Rosendo, Pereira, Virgínia L.A., Abreu, Dayse L.C., Gouvea, Raquel, Santos, Lídia M.M.
Tipo de documento: Artigo
Idioma: por
Título da fonte: Brazilian Journal of Veterinary Medicine
Texto Completo: https://rbmv.org/BJVM/article/view/523
Resumo: ABSTRACT. Machado L.S., do Nascimento E.R., Pereira V.L.A., Abreu D.L.C., Gouvea R. & Santos L.M.M. 2014. [Escherichia coli in broiler chickens with airsacculitis.] Escherichia coli em frangos de corte com aerossaculite. Revista Brasileira de Medicina Veterinária, 36(3):261-265, 2014. Departamento de Medicina Veterinária Preventiva e Saúde Pública, Faculdade de Veterinária, Universidade Federal Fluminense, Rua Dr. Vital Brazil Filho 64, Vital Brazil, Niterói, RJ 24230-340, Brazil. E-mail: leandromachadovet@yahoo.com.br The Brazilian poultry industry grows each year and becomes increasingly representative in the production and export of products. The health care with poultry have accompanied and favored this evolution, however, respiratory agents that affect the weight and carcass quality, continue to cause great damage to the poultry industry. Airsacculitis is considered the main cause of total and partial condemnation of carcasses of broilers, and has been attributed to Mycoplasmosis mostly caused by Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) and Escherichia coli. The aim of this study was to relate the positivity of MG / MS and E. coli detected by PCR as a risk factor for airsacculitis in condemnation of broilers in Health Inspection Service. We studied 30 broiler poultry slaughtered in a slaughterhouse under Federal Sanitary Inspection, located in the State of Rio de Janeiro. 30 chickens were randomly collected from different lots and tracheas obtained in each PCR. DNA was extracted by phenol-chloroform method and amplified using pairs of “primer”specific for MG, MS and E. coli. Of the 30 chickens analyzed by PCR, 30% (9/30) had lesions in air sacs. None of the birds showed infection with MG and/or MS PCR, however 33.3% (3/9) birds were positive for airsacculitis iss gene from E.coli. E.coli found in broiler chickens that were negative for mycoplasma airsacculitis, implying the presence of such bacteria may be sufficient to cause respiratory problems in broilers and no influence on diagnosis by type of material collection biological.
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spelling Escherichia coli in broiler chickens with airsacculitisEscherichia coli em frangos de corte com aerossaculiteFrangos de corteaerossaculitemicoplasmasEscherichia coliPCRBroilersairsacculitisMycoplasmaPCRABSTRACT. Machado L.S., do Nascimento E.R., Pereira V.L.A., Abreu D.L.C., Gouvea R. & Santos L.M.M. 2014. [Escherichia coli in broiler chickens with airsacculitis.] Escherichia coli em frangos de corte com aerossaculite. Revista Brasileira de Medicina Veterinária, 36(3):261-265, 2014. Departamento de Medicina Veterinária Preventiva e Saúde Pública, Faculdade de Veterinária, Universidade Federal Fluminense, Rua Dr. Vital Brazil Filho 64, Vital Brazil, Niterói, RJ 24230-340, Brazil. E-mail: leandromachadovet@yahoo.com.br The Brazilian poultry industry grows each year and becomes increasingly representative in the production and export of products. The health care with poultry have accompanied and favored this evolution, however, respiratory agents that affect the weight and carcass quality, continue to cause great damage to the poultry industry. Airsacculitis is considered the main cause of total and partial condemnation of carcasses of broilers, and has been attributed to Mycoplasmosis mostly caused by Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) and Escherichia coli. The aim of this study was to relate the positivity of MG / MS and E. coli detected by PCR as a risk factor for airsacculitis in condemnation of broilers in Health Inspection Service. We studied 30 broiler poultry slaughtered in a slaughterhouse under Federal Sanitary Inspection, located in the State of Rio de Janeiro. 30 chickens were randomly collected from different lots and tracheas obtained in each PCR. DNA was extracted by phenol-chloroform method and amplified using pairs of “primer”specific for MG, MS and E. coli. Of the 30 chickens analyzed by PCR, 30% (9/30) had lesions in air sacs. None of the birds showed infection with MG and/or MS PCR, however 33.3% (3/9) birds were positive for airsacculitis iss gene from E.coli. E.coli found in broiler chickens that were negative for mycoplasma airsacculitis, implying the presence of such bacteria may be sufficient to cause respiratory problems in broilers and no influence on diagnosis by type of material collection biological.A Indústria avícola brasileira cresce anualmente e se torna cada vez mais representativa na produção e exportação dos seus produtos. Os cuidados com a sanidade avícola têm acompanhado e favorecido essa evolução, entretanto, agentes respiratórios que afetam o peso e a qualidade da carcaça, continuam a provocar grandes prejuízos à avicultura industrial. Aerossaculite é considerada a principal causa da condenação total e parcial de carcaças de frangos de corte, e tem sido atribuída às Micoplasmoses, causadas principalmente pelo Mycoplasma gallisepticum (MG) e Mycoplasma synoviae (MS) e por Escherichia coli. O objetivo do presente estudo foi o de relacionar a positividade por MG/MS e E. coli detectado pela PCR como fator de risco de aerossaculite na condenação de frangos de corte na Inspeção Sanitária Federal. Foram estudados 30 frangos de corte abatidos em um matadouro avícola sob Inspeção Sanitária Federal, localizado no Estado do Rio de Janeiro. Foram coletados aleatoriamente 30 frangos de diferentes lotes e obtidos traquéias em cada um deles para PCR. O DNA foi extraído pelo método de fenol-clorofórmio e amplificado com pares de “primers” específicos para MG, MS e E.coli. Dos 30 frangos analisados pela PCR, 30% (9/30) apresentaram lesões nos sacos aé- reos. Nenhuma das aves apresentou infecção por MG e/ou MS pela PCR, entretanto 33,3% (3/9) aves com aerossaculite apresentaram positividade para gene iss de E. coli. E. coli foi encontrada em frangos de corte negativos para micoplasmas que apresentaram aerossaculite, levando a crer que a presença dessa bacteria pode ser suficiente para causar problemas no sistema respiratório de frangos de corte e não houve influencia no diagnóstico através do tipo de colheita do material biológico.Sociedade de Medicina Veterinária do Estado do Rio de Janeiro.2014-09-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionpeer reviewedAvaliado pelos paresapplication/pdfhttps://rbmv.org/BJVM/article/view/523Brazilian Journal of Veterinary Medicine; Vol. 36 No. 3 (2014); 261-265Revista Brasileira de Medicina Veterinária; v. 36 n. 3 (2014); 261-2652527-21790100-2430reponame:Brazilian Journal of Veterinary Medicineinstname:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)instacron:SBMVporhttps://rbmv.org/BJVM/article/view/523/396Machado, Leandro S.do Nascimento, Elmiro RosendoPereira, Virgínia L.A.Abreu, Dayse L.C.Gouvea, RaquelSantos, Lídia M.M.info:eu-repo/semantics/openAccess2020-12-23T17:32:32Zoai:ojs.rbmv.org:article/523Revistahttps://rbmv.org/BJVMONGhttps://rbmv.org/BJVM/oaicontato.rbmv@gmail.com2527-21790100-2430opendoar:2020-12-23T17:32:32Brazilian Journal of Veterinary Medicine - Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)false
dc.title.none.fl_str_mv Escherichia coli in broiler chickens with airsacculitis
Escherichia coli em frangos de corte com aerossaculite
title Escherichia coli in broiler chickens with airsacculitis
spellingShingle Escherichia coli in broiler chickens with airsacculitis
Machado, Leandro S.
Frangos de corte
aerossaculite
micoplasmas
Escherichia coli
PCR
Broilers
airsacculitis
Mycoplasma
PCR
title_short Escherichia coli in broiler chickens with airsacculitis
title_full Escherichia coli in broiler chickens with airsacculitis
title_fullStr Escherichia coli in broiler chickens with airsacculitis
title_full_unstemmed Escherichia coli in broiler chickens with airsacculitis
title_sort Escherichia coli in broiler chickens with airsacculitis
author Machado, Leandro S.
author_facet Machado, Leandro S.
do Nascimento, Elmiro Rosendo
Pereira, Virgínia L.A.
Abreu, Dayse L.C.
Gouvea, Raquel
Santos, Lídia M.M.
author_role author
author2 do Nascimento, Elmiro Rosendo
Pereira, Virgínia L.A.
Abreu, Dayse L.C.
Gouvea, Raquel
Santos, Lídia M.M.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Machado, Leandro S.
do Nascimento, Elmiro Rosendo
Pereira, Virgínia L.A.
Abreu, Dayse L.C.
Gouvea, Raquel
Santos, Lídia M.M.
dc.subject.por.fl_str_mv Frangos de corte
aerossaculite
micoplasmas
Escherichia coli
PCR
Broilers
airsacculitis
Mycoplasma
PCR
topic Frangos de corte
aerossaculite
micoplasmas
Escherichia coli
PCR
Broilers
airsacculitis
Mycoplasma
PCR
description ABSTRACT. Machado L.S., do Nascimento E.R., Pereira V.L.A., Abreu D.L.C., Gouvea R. & Santos L.M.M. 2014. [Escherichia coli in broiler chickens with airsacculitis.] Escherichia coli em frangos de corte com aerossaculite. Revista Brasileira de Medicina Veterinária, 36(3):261-265, 2014. Departamento de Medicina Veterinária Preventiva e Saúde Pública, Faculdade de Veterinária, Universidade Federal Fluminense, Rua Dr. Vital Brazil Filho 64, Vital Brazil, Niterói, RJ 24230-340, Brazil. E-mail: leandromachadovet@yahoo.com.br The Brazilian poultry industry grows each year and becomes increasingly representative in the production and export of products. The health care with poultry have accompanied and favored this evolution, however, respiratory agents that affect the weight and carcass quality, continue to cause great damage to the poultry industry. Airsacculitis is considered the main cause of total and partial condemnation of carcasses of broilers, and has been attributed to Mycoplasmosis mostly caused by Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) and Escherichia coli. The aim of this study was to relate the positivity of MG / MS and E. coli detected by PCR as a risk factor for airsacculitis in condemnation of broilers in Health Inspection Service. We studied 30 broiler poultry slaughtered in a slaughterhouse under Federal Sanitary Inspection, located in the State of Rio de Janeiro. 30 chickens were randomly collected from different lots and tracheas obtained in each PCR. DNA was extracted by phenol-chloroform method and amplified using pairs of “primer”specific for MG, MS and E. coli. Of the 30 chickens analyzed by PCR, 30% (9/30) had lesions in air sacs. None of the birds showed infection with MG and/or MS PCR, however 33.3% (3/9) birds were positive for airsacculitis iss gene from E.coli. E.coli found in broiler chickens that were negative for mycoplasma airsacculitis, implying the presence of such bacteria may be sufficient to cause respiratory problems in broilers and no influence on diagnosis by type of material collection biological.
publishDate 2014
dc.date.none.fl_str_mv 2014-09-10
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
peer reviewed
Avaliado pelos pares
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rbmv.org/BJVM/article/view/523
url https://rbmv.org/BJVM/article/view/523
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://rbmv.org/BJVM/article/view/523/396
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Sociedade de Medicina Veterinária do Estado do Rio de Janeiro.
publisher.none.fl_str_mv Sociedade de Medicina Veterinária do Estado do Rio de Janeiro.
dc.source.none.fl_str_mv Brazilian Journal of Veterinary Medicine; Vol. 36 No. 3 (2014); 261-265
Revista Brasileira de Medicina Veterinária; v. 36 n. 3 (2014); 261-265
2527-2179
0100-2430
reponame:Brazilian Journal of Veterinary Medicine
instname:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)
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instname_str Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)
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institution SBMV
reponame_str Brazilian Journal of Veterinary Medicine
collection Brazilian Journal of Veterinary Medicine
repository.name.fl_str_mv Brazilian Journal of Veterinary Medicine - Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)
repository.mail.fl_str_mv contato.rbmv@gmail.com
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