Transovarial transmission and finding of Trypanosoma rhipicephalis in the hemolymph of Rhipicephalus sanguineus sensu lato

Detalhes bibliográficos
Autor(a) principal: da Fonseca, Adivaldo Henrique
Data de Publicação: 2019
Outros Autores: Kaulich, Yasmim, Ribeiro, Carolina Marotta, Valim, Jaqueline Rodrigues de Almeida, dos Santos, Juliana Ferreira, dos Santos, Thays Figueiroa, da Silva, Cláudia Bezerra
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Veterinary Medicine
Texto Completo: https://rbmv.org/BJVM/article/view/1068
Resumo: Trypanosoma rhipicephalis Marotta et al. (2018) is a species isolated from Rhipicephalus microplus ticks collected from native bovine Seropédica, in the state of Rio de Janeiro. This study aimed to investigate the interaction of the tick Rhipicephalus sanguineus s. l. with T. rhipicephalis by in vitro artificial feeding. Eight females of R. sanguineus s. l. partially fed on rabbits. Tick infection was performed by an artificial feeding system using plastic tips for 12 hours. Canine blood used for feeding the ticks test group was previously infected with T. rhipicephalis 108/mL. The hemolymph smear test was performed in all females after experimental infection. The daily posture was collected and organized in pools of each female per posture day. The eggs were divided into three groups, the first group for eggs PCR, the second for hatching and larval PCR, and the third group of eggs for isolation in cell culture. The evaluation of the presence of DNA in the macerated eggs of experimentally infected ticks showed two positive PCR samples. The evaluation of the presence of DNA in experimentally infected females showed all samples tested positive. For the hemolymph test, tick number 7 presented epimastigote developmental forms and amastigotes of T. rhipicephalis. Experimental infection by artificial feeding proved to be a suitable tool to study the interaction of T. rhipicephalis in R. sanguineus s. l. ticks. The results show the transovarial transmission of T. rhipicephalis by R. sanguineus s. l., as well as the interaction of the protozoan in the organism of this tick species.
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spelling Transovarial transmission and finding of Trypanosoma rhipicephalis in the hemolymph of Rhipicephalus sanguineus sensu latoTransmissão transovariana e achado de Trypanosoma rhipicephalis na hemolinfa de Rhipicephalus sanguineus sensu latoCarrapato, alimentação artificial, tripanosomatídeos.Ticks, artificial feeding, trypanosomatids.Trypanosoma rhipicephalis Marotta et al. (2018) is a species isolated from Rhipicephalus microplus ticks collected from native bovine Seropédica, in the state of Rio de Janeiro. This study aimed to investigate the interaction of the tick Rhipicephalus sanguineus s. l. with T. rhipicephalis by in vitro artificial feeding. Eight females of R. sanguineus s. l. partially fed on rabbits. Tick infection was performed by an artificial feeding system using plastic tips for 12 hours. Canine blood used for feeding the ticks test group was previously infected with T. rhipicephalis 108/mL. The hemolymph smear test was performed in all females after experimental infection. The daily posture was collected and organized in pools of each female per posture day. The eggs were divided into three groups, the first group for eggs PCR, the second for hatching and larval PCR, and the third group of eggs for isolation in cell culture. The evaluation of the presence of DNA in the macerated eggs of experimentally infected ticks showed two positive PCR samples. The evaluation of the presence of DNA in experimentally infected females showed all samples tested positive. For the hemolymph test, tick number 7 presented epimastigote developmental forms and amastigotes of T. rhipicephalis. Experimental infection by artificial feeding proved to be a suitable tool to study the interaction of T. rhipicephalis in R. sanguineus s. l. ticks. The results show the transovarial transmission of T. rhipicephalis by R. sanguineus s. l., as well as the interaction of the protozoan in the organism of this tick species.Trypanossoma rhipicephalis Marotta et al. (2018) é uma espécie isolada do carrapato Rhipicephalus microplus coletado de bovino nativo de Seropédica, estado do Rio de Janeiro. O presente estudo teve como objetivo investigar a interação do carrapato Rhipicephalus sanguineus s.l. com T. rhipicephalis por meio da alimentação artificial in vitro. Foram utilizadas 8 fêmeas de R. sanguineus s. l. parcialmente alimentadas em coelhos. A infecção dos carrapatos foi realizada por meio de um sistema de alimentação artificial utilizando ponteiras plásticas por um período de 12 horas. O sangue canino utilizado para alimentar os carrapatos do grupo teste foi previamente infectado com 108 de T. rhipicephalis/mL. O teste de esfregaço de hemolinfa foi realizado em todas as fêmeas após a infecção experimental. A postura diária foi coletada e organizada em pools de cada fêmea por dia de postura. Os ovos foram separados em 3 grupos, sendo o primeiro grupo para a PCR dos ovos, o segundo para sua eclosão e PCR das larvas e o terceiro grupo para isolamento dos ovos em cultivo celular. A avaliação da presença de DNA no macerado de ovos dos carrapatos infectados experimentalmente mostrou duas amostras positivas na PCR. A avaliação da presença de DNA nas fêmeas infectadas experimentalmente mostrou todas as amostras do grupo teste positivas. Pelo teste de hemolinfa, o carrapato número 7 apresentou formas evolutivas epimastigota e amastigotas de T. rhipicephalis. A infecção experimental por meio da alimentação artificial mostrou-se uma ferramenta adequada para estudar a interação de T. rhipicephalis em R. sanguineus s. l. Os resultados comprovam a transmissão transovariana do T. rhipicephalis por R. sanguineus s. l., assim como a interação do protozoário no organismo desta espécie de carrapato.Sociedade de Medicina Veterinária do Estado do Rio de Janeiro.2019-09-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionpeer reviewedAvaliado pelos paresapplication/pdfhttps://rbmv.org/BJVM/article/view/106810.29374/2527-2179.bjvm106819Brazilian Journal of Veterinary Medicine; Vol. 41 No. 1 (2019); e106819Revista Brasileira de Medicina Veterinária; v. 41 n. 1 (2019); e1068192527-21790100-2430reponame:Brazilian Journal of Veterinary Medicineinstname:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)instacron:SBMVenghttps://rbmv.org/BJVM/article/view/1068/961Copyright (c) 2019 Adivaldo Henrique da Fonseca, Yasmim Kaulich, Carolina Marotta Ribeiro, Jaqueline Rodrigues de Almeida Valim, Juliana Ferreira dos Santos, Thays Figueiroa dos Santos, Cláudia Bezerra da Silvahttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessda Fonseca, Adivaldo HenriqueKaulich, YasmimRibeiro, Carolina Marotta Valim, Jaqueline Rodrigues de Almeidados Santos, Juliana Ferreirados Santos, Thays Figueiroa da Silva, Cláudia Bezerra2021-05-12T16:38:45Zoai:ojs.rbmv.org:article/1068Revistahttps://rbmv.org/BJVMONGhttps://rbmv.org/BJVM/oaicontato.rbmv@gmail.com2527-21790100-2430opendoar:2021-05-12T16:38:45Brazilian Journal of Veterinary Medicine - Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)false
dc.title.none.fl_str_mv Transovarial transmission and finding of Trypanosoma rhipicephalis in the hemolymph of Rhipicephalus sanguineus sensu lato
Transmissão transovariana e achado de Trypanosoma rhipicephalis na hemolinfa de Rhipicephalus sanguineus sensu lato
title Transovarial transmission and finding of Trypanosoma rhipicephalis in the hemolymph of Rhipicephalus sanguineus sensu lato
spellingShingle Transovarial transmission and finding of Trypanosoma rhipicephalis in the hemolymph of Rhipicephalus sanguineus sensu lato
da Fonseca, Adivaldo Henrique
Carrapato, alimentação artificial, tripanosomatídeos.
Ticks, artificial feeding, trypanosomatids.
title_short Transovarial transmission and finding of Trypanosoma rhipicephalis in the hemolymph of Rhipicephalus sanguineus sensu lato
title_full Transovarial transmission and finding of Trypanosoma rhipicephalis in the hemolymph of Rhipicephalus sanguineus sensu lato
title_fullStr Transovarial transmission and finding of Trypanosoma rhipicephalis in the hemolymph of Rhipicephalus sanguineus sensu lato
title_full_unstemmed Transovarial transmission and finding of Trypanosoma rhipicephalis in the hemolymph of Rhipicephalus sanguineus sensu lato
title_sort Transovarial transmission and finding of Trypanosoma rhipicephalis in the hemolymph of Rhipicephalus sanguineus sensu lato
author da Fonseca, Adivaldo Henrique
author_facet da Fonseca, Adivaldo Henrique
Kaulich, Yasmim
Ribeiro, Carolina Marotta
Valim, Jaqueline Rodrigues de Almeida
dos Santos, Juliana Ferreira
dos Santos, Thays Figueiroa
da Silva, Cláudia Bezerra
author_role author
author2 Kaulich, Yasmim
Ribeiro, Carolina Marotta
Valim, Jaqueline Rodrigues de Almeida
dos Santos, Juliana Ferreira
dos Santos, Thays Figueiroa
da Silva, Cláudia Bezerra
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv da Fonseca, Adivaldo Henrique
Kaulich, Yasmim
Ribeiro, Carolina Marotta
Valim, Jaqueline Rodrigues de Almeida
dos Santos, Juliana Ferreira
dos Santos, Thays Figueiroa
da Silva, Cláudia Bezerra
dc.subject.por.fl_str_mv Carrapato, alimentação artificial, tripanosomatídeos.
Ticks, artificial feeding, trypanosomatids.
topic Carrapato, alimentação artificial, tripanosomatídeos.
Ticks, artificial feeding, trypanosomatids.
description Trypanosoma rhipicephalis Marotta et al. (2018) is a species isolated from Rhipicephalus microplus ticks collected from native bovine Seropédica, in the state of Rio de Janeiro. This study aimed to investigate the interaction of the tick Rhipicephalus sanguineus s. l. with T. rhipicephalis by in vitro artificial feeding. Eight females of R. sanguineus s. l. partially fed on rabbits. Tick infection was performed by an artificial feeding system using plastic tips for 12 hours. Canine blood used for feeding the ticks test group was previously infected with T. rhipicephalis 108/mL. The hemolymph smear test was performed in all females after experimental infection. The daily posture was collected and organized in pools of each female per posture day. The eggs were divided into three groups, the first group for eggs PCR, the second for hatching and larval PCR, and the third group of eggs for isolation in cell culture. The evaluation of the presence of DNA in the macerated eggs of experimentally infected ticks showed two positive PCR samples. The evaluation of the presence of DNA in experimentally infected females showed all samples tested positive. For the hemolymph test, tick number 7 presented epimastigote developmental forms and amastigotes of T. rhipicephalis. Experimental infection by artificial feeding proved to be a suitable tool to study the interaction of T. rhipicephalis in R. sanguineus s. l. ticks. The results show the transovarial transmission of T. rhipicephalis by R. sanguineus s. l., as well as the interaction of the protozoan in the organism of this tick species.
publishDate 2019
dc.date.none.fl_str_mv 2019-09-30
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
peer reviewed
Avaliado pelos pares
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rbmv.org/BJVM/article/view/1068
10.29374/2527-2179.bjvm106819
url https://rbmv.org/BJVM/article/view/1068
identifier_str_mv 10.29374/2527-2179.bjvm106819
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://rbmv.org/BJVM/article/view/1068/961
dc.rights.driver.fl_str_mv http://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Sociedade de Medicina Veterinária do Estado do Rio de Janeiro.
publisher.none.fl_str_mv Sociedade de Medicina Veterinária do Estado do Rio de Janeiro.
dc.source.none.fl_str_mv Brazilian Journal of Veterinary Medicine; Vol. 41 No. 1 (2019); e106819
Revista Brasileira de Medicina Veterinária; v. 41 n. 1 (2019); e106819
2527-2179
0100-2430
reponame:Brazilian Journal of Veterinary Medicine
instname:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)
instacron:SBMV
instname_str Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)
instacron_str SBMV
institution SBMV
reponame_str Brazilian Journal of Veterinary Medicine
collection Brazilian Journal of Veterinary Medicine
repository.name.fl_str_mv Brazilian Journal of Veterinary Medicine - Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)
repository.mail.fl_str_mv contato.rbmv@gmail.com
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