Detecção de Brucella canis no sangue, urina e sêmen de cão naturalmente infectado através de PCR
Autor(a) principal: | |
---|---|
Data de Publicação: | 2018 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Veterinary Medicine |
Texto Completo: | https://rbmv.org/BJVM/article/view/27 |
Resumo: | Canine Brucellosis is a bacterial infectious disease that affects dogs around the world. The laboratory diagnosis is based on direct and indirect methods and should be performed when there is evidence from clinical and epidemiological signs. In this study, we observed scrotal dermatitis recurrent and resistant to treatment in six years dog, Golden Retriever. An AGID serological test for brucellosis was positive. However, serological tests may show unspecific reactions and false positive results. Blood culture is indicated as a confirmatory test. The result of the culture was negative in all materials tested (blood cultures, urine culture and semen in tryptose broth and blood agar). Thus, the blood, urine and semen samples were tested by PCR and all samples tested were positive. After castration, the histologic evaluation showed inflammatory infiltrate in the epididymis. Vacuolar degeneration of the germinal epithelium of the seminiferous tubules was observed in the testicular parenchyma without testicular atrophy. Morphological semen evaluation showed sperm abnormalities as strongly folded tail, loose heads, curly tails, head-head, corroborating the diagnosis of brucellosis. PCR was crucial to the canine brucellosis final diagnosis, demonstrating the high sensitivity and efficiency of this test, suggesting that PCR should be included as a confirmatory test, aimed at preventing the spread of this important zoonosis. |
id |
SBMV-1_48b4e02ffac07e1683024d15d66f92a5 |
---|---|
oai_identifier_str |
oai:ojs.rbmv.org:article/27 |
network_acronym_str |
SBMV-1 |
network_name_str |
Brazilian Journal of Veterinary Medicine |
repository_id_str |
|
spelling |
Detecção de Brucella canis no sangue, urina e sêmen de cão naturalmente infectado através de PCRDetection of Brucella canis in blood, urine and seminal fluid of a naturally infected dog by PCRReaction Polymerase Chainblood cultureAGIDCanine BrucellosisCanine Brucellosis is a bacterial infectious disease that affects dogs around the world. The laboratory diagnosis is based on direct and indirect methods and should be performed when there is evidence from clinical and epidemiological signs. In this study, we observed scrotal dermatitis recurrent and resistant to treatment in six years dog, Golden Retriever. An AGID serological test for brucellosis was positive. However, serological tests may show unspecific reactions and false positive results. Blood culture is indicated as a confirmatory test. The result of the culture was negative in all materials tested (blood cultures, urine culture and semen in tryptose broth and blood agar). Thus, the blood, urine and semen samples were tested by PCR and all samples tested were positive. After castration, the histologic evaluation showed inflammatory infiltrate in the epididymis. Vacuolar degeneration of the germinal epithelium of the seminiferous tubules was observed in the testicular parenchyma without testicular atrophy. Morphological semen evaluation showed sperm abnormalities as strongly folded tail, loose heads, curly tails, head-head, corroborating the diagnosis of brucellosis. PCR was crucial to the canine brucellosis final diagnosis, demonstrating the high sensitivity and efficiency of this test, suggesting that PCR should be included as a confirmatory test, aimed at preventing the spread of this important zoonosis.A brucelose canina é uma doença bacteriana, infectocontagiosa que acomete cães de todo o mundo.O diagnóstico laboratorial é baseado em métodos diretos e indiretos e deve ser realizado quando há evidências de sinais clínicos e epidemiológicos. Neste estudo, observou-se dermatite escrotal recorrente e resistente a tratamento em cão de 6 anos, da raça Golden Retriever. Realizou-se teste sorológico IDGA para brucelose, que foi positivo. Porém, os testes sorológicos podem apresentar reações inespecíficas e resultados falsos positivos. Como teste confirmatório é indicado o exame da hemocultura. Realizou-se hemocultura, cultura de urina e sêmen em caldo triptose e ágar sangue. Em todos os materiais o resultado da cultura foi negativo. Desta forma, foram testadas amostras de sangue, urina e sêmen, bem como amostras enriquecidas em caldo triptose, seguidas de PCR, que apresentaram positividade em todas as amostras testadas. O animal foi castrado e na avaliação histológica, os testículos apresentaram infiltrado inflamatório em epidídimo. No parênquima testicular observou-se degeneração vacuolar do epitélio germinativo de túbulos seminíferos, sem atrofia testicular. A avaliação morfológica do sêmen evidenciou anormalidades dos espermatozóides, dentre elas, cauda fortemente dobrada, cabeças soltas, caudas enroladas, cabeça-cabeça, corroborando para o diagnóstico de brucelose. A PCR foi fundamental para o diagnóstico final da brucelose canina, comprovando a alta sensibilidade e eficácia do exame, sugerindo que a PCR deve ser incluída como teste confirmatório, visando evitar a propagação desta importante zoonoseSociedade de Medicina Veterinária do Estado do Rio de Janeiro.2018-09-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionpeer reviewedAvaliado pelos paresapplication/pdfhttps://rbmv.org/BJVM/article/view/2710.29374/2527-2179.bjvm002718Brazilian Journal of Veterinary Medicine; Vol. 40 No. 1 (2018); e002718Revista Brasileira de Medicina Veterinária; v. 40 n. 1 (2018); e0027182527-21790100-2430reponame:Brazilian Journal of Veterinary Medicineinstname:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)instacron:SBMVenghttps://rbmv.org/BJVM/article/view/27/789Copyright (c) 2018 Fabiana Sperb Volkweis, Lilian Cristina Cavalcanti, Hélio Blume, Helvécio Leal Santos Júnior, Andrea Lazzari, Fernanda Mulinariinfo:eu-repo/semantics/openAccessVolkweis, Fabiana SperbCavalcanti, Lilian CristinaBlume, HélioSantos Júnior, Helvécio LealLazzari, AndreaMulinari, Fernanda2021-03-12T18:04:48Zoai:ojs.rbmv.org:article/27Revistahttps://rbmv.org/BJVMONGhttps://rbmv.org/BJVM/oaicontato.rbmv@gmail.com2527-21790100-2430opendoar:2021-03-12T18:04:48Brazilian Journal of Veterinary Medicine - Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)false |
dc.title.none.fl_str_mv |
Detecção de Brucella canis no sangue, urina e sêmen de cão naturalmente infectado através de PCR Detection of Brucella canis in blood, urine and seminal fluid of a naturally infected dog by PCR |
title |
Detecção de Brucella canis no sangue, urina e sêmen de cão naturalmente infectado através de PCR |
spellingShingle |
Detecção de Brucella canis no sangue, urina e sêmen de cão naturalmente infectado através de PCR Volkweis, Fabiana Sperb Reaction Polymerase Chain blood culture AGID Canine Brucellosis |
title_short |
Detecção de Brucella canis no sangue, urina e sêmen de cão naturalmente infectado através de PCR |
title_full |
Detecção de Brucella canis no sangue, urina e sêmen de cão naturalmente infectado através de PCR |
title_fullStr |
Detecção de Brucella canis no sangue, urina e sêmen de cão naturalmente infectado através de PCR |
title_full_unstemmed |
Detecção de Brucella canis no sangue, urina e sêmen de cão naturalmente infectado através de PCR |
title_sort |
Detecção de Brucella canis no sangue, urina e sêmen de cão naturalmente infectado através de PCR |
author |
Volkweis, Fabiana Sperb |
author_facet |
Volkweis, Fabiana Sperb Cavalcanti, Lilian Cristina Blume, Hélio Santos Júnior, Helvécio Leal Lazzari, Andrea Mulinari, Fernanda |
author_role |
author |
author2 |
Cavalcanti, Lilian Cristina Blume, Hélio Santos Júnior, Helvécio Leal Lazzari, Andrea Mulinari, Fernanda |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Volkweis, Fabiana Sperb Cavalcanti, Lilian Cristina Blume, Hélio Santos Júnior, Helvécio Leal Lazzari, Andrea Mulinari, Fernanda |
dc.subject.por.fl_str_mv |
Reaction Polymerase Chain blood culture AGID Canine Brucellosis |
topic |
Reaction Polymerase Chain blood culture AGID Canine Brucellosis |
description |
Canine Brucellosis is a bacterial infectious disease that affects dogs around the world. The laboratory diagnosis is based on direct and indirect methods and should be performed when there is evidence from clinical and epidemiological signs. In this study, we observed scrotal dermatitis recurrent and resistant to treatment in six years dog, Golden Retriever. An AGID serological test for brucellosis was positive. However, serological tests may show unspecific reactions and false positive results. Blood culture is indicated as a confirmatory test. The result of the culture was negative in all materials tested (blood cultures, urine culture and semen in tryptose broth and blood agar). Thus, the blood, urine and semen samples were tested by PCR and all samples tested were positive. After castration, the histologic evaluation showed inflammatory infiltrate in the epididymis. Vacuolar degeneration of the germinal epithelium of the seminiferous tubules was observed in the testicular parenchyma without testicular atrophy. Morphological semen evaluation showed sperm abnormalities as strongly folded tail, loose heads, curly tails, head-head, corroborating the diagnosis of brucellosis. PCR was crucial to the canine brucellosis final diagnosis, demonstrating the high sensitivity and efficiency of this test, suggesting that PCR should be included as a confirmatory test, aimed at preventing the spread of this important zoonosis. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-09-03 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion peer reviewed Avaliado pelos pares |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rbmv.org/BJVM/article/view/27 10.29374/2527-2179.bjvm002718 |
url |
https://rbmv.org/BJVM/article/view/27 |
identifier_str_mv |
10.29374/2527-2179.bjvm002718 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rbmv.org/BJVM/article/view/27/789 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade de Medicina Veterinária do Estado do Rio de Janeiro. |
publisher.none.fl_str_mv |
Sociedade de Medicina Veterinária do Estado do Rio de Janeiro. |
dc.source.none.fl_str_mv |
Brazilian Journal of Veterinary Medicine; Vol. 40 No. 1 (2018); e002718 Revista Brasileira de Medicina Veterinária; v. 40 n. 1 (2018); e002718 2527-2179 0100-2430 reponame:Brazilian Journal of Veterinary Medicine instname:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ) instacron:SBMV |
instname_str |
Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ) |
instacron_str |
SBMV |
institution |
SBMV |
reponame_str |
Brazilian Journal of Veterinary Medicine |
collection |
Brazilian Journal of Veterinary Medicine |
repository.name.fl_str_mv |
Brazilian Journal of Veterinary Medicine - Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ) |
repository.mail.fl_str_mv |
contato.rbmv@gmail.com |
_version_ |
1798313106300993536 |