Cryptosporidium diagnosis by qPCR in cats at Rio de Janeiro state, Brazil
Autor(a) principal: | |
---|---|
Data de Publicação: | 2016 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Brazilian Journal of Veterinary Medicine |
Texto Completo: | https://rbmv.org/BJVM/article/view/170 |
Resumo: | ABSTRACT. Carrasco L.P.S., Oliveira R.L.S., Moreira C.M.R., Santos C.R.G.R., Corgozinho K.B. & Souza H.J.M. [Cryptosporidium diagnosis by qPCR in cats at Rio de Janeiro state, Brazil.] Diagnóstico de Cryptosporidium spp. pela técnica de qPCR em gatos no estado do Rio de Janeiro, Brasil. Revista Brasileira de Medicina Veterinária, 38(Supl.):22-26, 2016. Programa de Pós-Graduação em MedicinaVeteriná- ria, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, BR-465, Km 7, Seropédica, RJ 23890-000, Brasil. E-mail: carrasco.lara@gmail.com Cryptosporidium spp. is recognized as an important etiologic agent of diarrhea in many countries. The aim of this study was to detect the presence of DNA of the parasite Cryptosporidium spp. in feces of cats with history of chronic diarrhea attended in the Feline Medicine Sector of the Veterinary Hospital of the Federal Rural University of Rio de Janeiro, by the polymerase chain reaction technique in real time (RT-PCR). In this study, 100 animals were admitted, of any breed or sex and from 8 weeks of age. As inclusion criteria, patients had to have diarrhea history for more than three weeks, with little success of clinical response to previously established therapies. From the samples obtained by collecting via washing the animal colon and spontaneous defecation, methods of direct examination of the feces, centrifugal flotation technique and real-time PCR were carried out. Of all cats selected for this study, 10% showed infection by Cryptosporidium spp. Most positive animals were aged over one year (70%) and only 30% had up to one year old. Cats were 50% purebred and 50% were domestic short hair cats. The clinical signs presented by these cats at the time of consultation were diarrhea (60%) and prolapsed rectum (40%). Four animals had co-infections with other enteropathogens (40%), such as Giardia, Toxocara sp. or Tritrichomonas fetus alone or combined. We concluded that infection by Cryptosporidium spp. is an important differential diagnosis in cats with chronic diarrhea, in cats of all ages and regardless of whether purebred or not, may have the infection and co-infection with other enteric pathogens needs to be investigated in all cases. |
id |
SBMV-1_83a3def8436a69b770da57a1c6549be1 |
---|---|
oai_identifier_str |
oai:ojs.rbmv.org:article/170 |
network_acronym_str |
SBMV-1 |
network_name_str |
Brazilian Journal of Veterinary Medicine |
repository_id_str |
|
spelling |
Cryptosporidium diagnosis by qPCR in cats at Rio de Janeiro state, BrazilDiagnóstico de Cryptosporidium spp. pela técnica de qPCR em gatos no estado do Rio de Janeiro, BrasilGatosdiarreiaCryptosporidium sppCatsdiarrheaCryptosporidium sppABSTRACT. Carrasco L.P.S., Oliveira R.L.S., Moreira C.M.R., Santos C.R.G.R., Corgozinho K.B. & Souza H.J.M. [Cryptosporidium diagnosis by qPCR in cats at Rio de Janeiro state, Brazil.] Diagnóstico de Cryptosporidium spp. pela técnica de qPCR em gatos no estado do Rio de Janeiro, Brasil. Revista Brasileira de Medicina Veterinária, 38(Supl.):22-26, 2016. Programa de Pós-Graduação em MedicinaVeteriná- ria, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, BR-465, Km 7, Seropédica, RJ 23890-000, Brasil. E-mail: carrasco.lara@gmail.com Cryptosporidium spp. is recognized as an important etiologic agent of diarrhea in many countries. The aim of this study was to detect the presence of DNA of the parasite Cryptosporidium spp. in feces of cats with history of chronic diarrhea attended in the Feline Medicine Sector of the Veterinary Hospital of the Federal Rural University of Rio de Janeiro, by the polymerase chain reaction technique in real time (RT-PCR). In this study, 100 animals were admitted, of any breed or sex and from 8 weeks of age. As inclusion criteria, patients had to have diarrhea history for more than three weeks, with little success of clinical response to previously established therapies. From the samples obtained by collecting via washing the animal colon and spontaneous defecation, methods of direct examination of the feces, centrifugal flotation technique and real-time PCR were carried out. Of all cats selected for this study, 10% showed infection by Cryptosporidium spp. Most positive animals were aged over one year (70%) and only 30% had up to one year old. Cats were 50% purebred and 50% were domestic short hair cats. The clinical signs presented by these cats at the time of consultation were diarrhea (60%) and prolapsed rectum (40%). Four animals had co-infections with other enteropathogens (40%), such as Giardia, Toxocara sp. or Tritrichomonas fetus alone or combined. We concluded that infection by Cryptosporidium spp. is an important differential diagnosis in cats with chronic diarrhea, in cats of all ages and regardless of whether purebred or not, may have the infection and co-infection with other enteric pathogens needs to be investigated in all cases.Cryptosporidium spp. é reconhecido como um importante agente etiológico de diarreia em diversos países. O objetivo deste trabalho foi detectar a presença do ADN do protozoário Cryptosporidium spp. em fezes de gatos com histórico de diarreia crônica atendidos no Setor de Medicina Felina do Hospital Veterinário da Universidade Federal Rural do Rio de Janeiro, através da técnica de reação em cadeia da polimerase em tempo real (RT- -PCR). Neste estudo, foram admitidos 100 animais da espécie felina, de diferentes raças, de ambos os sexos e a partir de 8 semanas de idade. Como critério de inclusão, os pacientes deveriam apresentar histórico de diarreia a mais de três semanas, com pouco sucesso de resposta clínica para terapêuticas previamente instituídas. A partir das amostras de fezes obtidas por coleta via lavagem do cólon dos animais e pelo ato de defecação espontânea, foram realizados os métodos do exame direto das fezes, técnica de centrifugo flutuação e PCR em tempo real. Dos 100 gatos que foram selecionados para esta pesquisa, 10% apresentaram infecção por Cryptosporidium spp. A maioria dos animais positivos estavam na faixa etária acima de um ano (70%) e apenas 30% tinha até um ano de idade. Os gatos eram 50% de raça pura e 50% eram animais sem raça definida. Os sinais clínicos apresentados por esses gatos no momento da consulta foram diarreia (60%) e prolapso de reto (40%). Quatro animais apresentaram co- infecções com outros enteropatógenos (40%), um com Giardia sp., um com Toxocara sp., um com Tritrichomonas foetus e outro com Toxocara sp. e Giardia sp. Podemos concluir que a infecção por Cryptosporidium spp. é um importante diagnóstico diferencial em felinos com diarreia crônica, que gatos de qualquer idade e independente de ser de raça pura ou não, podem apresentar a infecção e que a co-infecção com outros enteropatógenos necessita ser investigada em todos casos.Sociedade de Medicina Veterinária do Estado do Rio de Janeiro.2016-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionpeer reviewedAvaliado pelos paresapplication/pdfhttps://rbmv.org/BJVM/article/view/170Brazilian Journal of Veterinary Medicine; Vol. 38 No. Supl.2 (2016); 22-26Revista Brasileira de Medicina Veterinária; v. 38 n. Supl.2 (2016); 22-262527-21790100-2430reponame:Brazilian Journal of Veterinary Medicineinstname:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)instacron:SBMVporhttps://rbmv.org/BJVM/article/view/170/110Carrasco, Lara Patrícia Santosde Oliveira, Renato Leão SáMoreira, Clarissa Martins do RioSantos, Carla Regina Gomes RodriguesCorgozinho, Kátia BarãoSouza, Heloisa Justen Moreirainfo:eu-repo/semantics/openAccess2020-12-23T17:34:13Zoai:ojs.rbmv.org:article/170Revistahttps://rbmv.org/BJVMONGhttps://rbmv.org/BJVM/oaicontato.rbmv@gmail.com2527-21790100-2430opendoar:2020-12-23T17:34:13Brazilian Journal of Veterinary Medicine - Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)false |
dc.title.none.fl_str_mv |
Cryptosporidium diagnosis by qPCR in cats at Rio de Janeiro state, Brazil Diagnóstico de Cryptosporidium spp. pela técnica de qPCR em gatos no estado do Rio de Janeiro, Brasil |
title |
Cryptosporidium diagnosis by qPCR in cats at Rio de Janeiro state, Brazil |
spellingShingle |
Cryptosporidium diagnosis by qPCR in cats at Rio de Janeiro state, Brazil Carrasco, Lara Patrícia Santos Gatos diarreia Cryptosporidium spp Cats diarrhea Cryptosporidium spp |
title_short |
Cryptosporidium diagnosis by qPCR in cats at Rio de Janeiro state, Brazil |
title_full |
Cryptosporidium diagnosis by qPCR in cats at Rio de Janeiro state, Brazil |
title_fullStr |
Cryptosporidium diagnosis by qPCR in cats at Rio de Janeiro state, Brazil |
title_full_unstemmed |
Cryptosporidium diagnosis by qPCR in cats at Rio de Janeiro state, Brazil |
title_sort |
Cryptosporidium diagnosis by qPCR in cats at Rio de Janeiro state, Brazil |
author |
Carrasco, Lara Patrícia Santos |
author_facet |
Carrasco, Lara Patrícia Santos de Oliveira, Renato Leão Sá Moreira, Clarissa Martins do Rio Santos, Carla Regina Gomes Rodrigues Corgozinho, Kátia Barão Souza, Heloisa Justen Moreira |
author_role |
author |
author2 |
de Oliveira, Renato Leão Sá Moreira, Clarissa Martins do Rio Santos, Carla Regina Gomes Rodrigues Corgozinho, Kátia Barão Souza, Heloisa Justen Moreira |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Carrasco, Lara Patrícia Santos de Oliveira, Renato Leão Sá Moreira, Clarissa Martins do Rio Santos, Carla Regina Gomes Rodrigues Corgozinho, Kátia Barão Souza, Heloisa Justen Moreira |
dc.subject.por.fl_str_mv |
Gatos diarreia Cryptosporidium spp Cats diarrhea Cryptosporidium spp |
topic |
Gatos diarreia Cryptosporidium spp Cats diarrhea Cryptosporidium spp |
description |
ABSTRACT. Carrasco L.P.S., Oliveira R.L.S., Moreira C.M.R., Santos C.R.G.R., Corgozinho K.B. & Souza H.J.M. [Cryptosporidium diagnosis by qPCR in cats at Rio de Janeiro state, Brazil.] Diagnóstico de Cryptosporidium spp. pela técnica de qPCR em gatos no estado do Rio de Janeiro, Brasil. Revista Brasileira de Medicina Veterinária, 38(Supl.):22-26, 2016. Programa de Pós-Graduação em MedicinaVeteriná- ria, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, BR-465, Km 7, Seropédica, RJ 23890-000, Brasil. E-mail: carrasco.lara@gmail.com Cryptosporidium spp. is recognized as an important etiologic agent of diarrhea in many countries. The aim of this study was to detect the presence of DNA of the parasite Cryptosporidium spp. in feces of cats with history of chronic diarrhea attended in the Feline Medicine Sector of the Veterinary Hospital of the Federal Rural University of Rio de Janeiro, by the polymerase chain reaction technique in real time (RT-PCR). In this study, 100 animals were admitted, of any breed or sex and from 8 weeks of age. As inclusion criteria, patients had to have diarrhea history for more than three weeks, with little success of clinical response to previously established therapies. From the samples obtained by collecting via washing the animal colon and spontaneous defecation, methods of direct examination of the feces, centrifugal flotation technique and real-time PCR were carried out. Of all cats selected for this study, 10% showed infection by Cryptosporidium spp. Most positive animals were aged over one year (70%) and only 30% had up to one year old. Cats were 50% purebred and 50% were domestic short hair cats. The clinical signs presented by these cats at the time of consultation were diarrhea (60%) and prolapsed rectum (40%). Four animals had co-infections with other enteropathogens (40%), such as Giardia, Toxocara sp. or Tritrichomonas fetus alone or combined. We concluded that infection by Cryptosporidium spp. is an important differential diagnosis in cats with chronic diarrhea, in cats of all ages and regardless of whether purebred or not, may have the infection and co-infection with other enteric pathogens needs to be investigated in all cases. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-11-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion peer reviewed Avaliado pelos pares |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rbmv.org/BJVM/article/view/170 |
url |
https://rbmv.org/BJVM/article/view/170 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://rbmv.org/BJVM/article/view/170/110 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade de Medicina Veterinária do Estado do Rio de Janeiro. |
publisher.none.fl_str_mv |
Sociedade de Medicina Veterinária do Estado do Rio de Janeiro. |
dc.source.none.fl_str_mv |
Brazilian Journal of Veterinary Medicine; Vol. 38 No. Supl.2 (2016); 22-26 Revista Brasileira de Medicina Veterinária; v. 38 n. Supl.2 (2016); 22-26 2527-2179 0100-2430 reponame:Brazilian Journal of Veterinary Medicine instname:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ) instacron:SBMV |
instname_str |
Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ) |
instacron_str |
SBMV |
institution |
SBMV |
reponame_str |
Brazilian Journal of Veterinary Medicine |
collection |
Brazilian Journal of Veterinary Medicine |
repository.name.fl_str_mv |
Brazilian Journal of Veterinary Medicine - Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ) |
repository.mail.fl_str_mv |
contato.rbmv@gmail.com |
_version_ |
1798313106888196096 |