Cryptosporidium diagnosis by qPCR in cats at Rio de Janeiro state, Brazil

Detalhes bibliográficos
Autor(a) principal: Carrasco, Lara Patrícia Santos
Data de Publicação: 2016
Outros Autores: de Oliveira, Renato Leão Sá, Moreira, Clarissa Martins do Rio, Santos, Carla Regina Gomes Rodrigues, Corgozinho, Kátia Barão, Souza, Heloisa Justen Moreira
Tipo de documento: Artigo
Idioma: por
Título da fonte: Brazilian Journal of Veterinary Medicine
Texto Completo: https://rbmv.org/BJVM/article/view/170
Resumo: ABSTRACT. Carrasco L.P.S., Oliveira R.L.S., Moreira C.M.R., Santos C.R.G.R., Corgozinho K.B. & Souza H.J.M. [Cryptosporidium diagnosis by qPCR in cats at Rio de Janeiro state, Brazil.] Diagnóstico de Cryptosporidium spp. pela técnica de qPCR em gatos no estado do Rio de Janeiro, Brasil. Revista Brasileira de Medicina Veterinária, 38(Supl.):22-26, 2016. Programa de Pós-Graduação em MedicinaVeteriná- ria, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, BR-465, Km 7, Seropédica, RJ 23890-000, Brasil. E-mail: carrasco.lara@gmail.com Cryptosporidium spp. is recognized as an important etiologic agent of diarrhea in many countries. The aim of this study was to detect the presence of DNA of the parasite Cryptosporidium spp. in feces of cats with history of chronic diarrhea attended in the Feline Medicine Sector of the Veterinary Hospital of the Federal Rural University of Rio de Janeiro, by the polymerase chain reaction technique in real time (RT-PCR). In this study, 100 animals were admitted, of any breed or sex and from 8 weeks of age. As inclusion criteria, patients had to have diarrhea history for more than three weeks, with little success of clinical response to previously established therapies. From the samples obtained by collecting via washing the animal colon and spontaneous defecation, methods of direct examination of the feces, centrifugal flotation technique and real-time PCR were carried out. Of all cats selected for this study, 10% showed infection by Cryptosporidium spp. Most positive animals were aged over one year (70%) and only 30% had up to one year old. Cats were 50% purebred and 50% were domestic short hair cats. The clinical signs presented by these cats at the time of consultation were diarrhea (60%) and prolapsed rectum (40%). Four animals had co-infections with other enteropathogens (40%), such as Giardia, Toxocara sp. or Tritrichomonas fetus alone or combined. We concluded that infection by Cryptosporidium spp. is an important differential diagnosis in cats with chronic diarrhea, in cats of all ages and regardless of whether purebred or not, may have the infection and co-infection with other enteric pathogens needs to be investigated in all cases.
id SBMV-1_83a3def8436a69b770da57a1c6549be1
oai_identifier_str oai:ojs.rbmv.org:article/170
network_acronym_str SBMV-1
network_name_str Brazilian Journal of Veterinary Medicine
repository_id_str
spelling Cryptosporidium diagnosis by qPCR in cats at Rio de Janeiro state, BrazilDiagnóstico de Cryptosporidium spp. pela técnica de qPCR em gatos no estado do Rio de Janeiro, BrasilGatosdiarreiaCryptosporidium sppCatsdiarrheaCryptosporidium sppABSTRACT. Carrasco L.P.S., Oliveira R.L.S., Moreira C.M.R., Santos C.R.G.R., Corgozinho K.B. & Souza H.J.M. [Cryptosporidium diagnosis by qPCR in cats at Rio de Janeiro state, Brazil.] Diagnóstico de Cryptosporidium spp. pela técnica de qPCR em gatos no estado do Rio de Janeiro, Brasil. Revista Brasileira de Medicina Veterinária, 38(Supl.):22-26, 2016. Programa de Pós-Graduação em MedicinaVeteriná- ria, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, BR-465, Km 7, Seropédica, RJ 23890-000, Brasil. E-mail: carrasco.lara@gmail.com Cryptosporidium spp. is recognized as an important etiologic agent of diarrhea in many countries. The aim of this study was to detect the presence of DNA of the parasite Cryptosporidium spp. in feces of cats with history of chronic diarrhea attended in the Feline Medicine Sector of the Veterinary Hospital of the Federal Rural University of Rio de Janeiro, by the polymerase chain reaction technique in real time (RT-PCR). In this study, 100 animals were admitted, of any breed or sex and from 8 weeks of age. As inclusion criteria, patients had to have diarrhea history for more than three weeks, with little success of clinical response to previously established therapies. From the samples obtained by collecting via washing the animal colon and spontaneous defecation, methods of direct examination of the feces, centrifugal flotation technique and real-time PCR were carried out. Of all cats selected for this study, 10% showed infection by Cryptosporidium spp. Most positive animals were aged over one year (70%) and only 30% had up to one year old. Cats were 50% purebred and 50% were domestic short hair cats. The clinical signs presented by these cats at the time of consultation were diarrhea (60%) and prolapsed rectum (40%). Four animals had co-infections with other enteropathogens (40%), such as Giardia, Toxocara sp. or Tritrichomonas fetus alone or combined. We concluded that infection by Cryptosporidium spp. is an important differential diagnosis in cats with chronic diarrhea, in cats of all ages and regardless of whether purebred or not, may have the infection and co-infection with other enteric pathogens needs to be investigated in all cases.Cryptosporidium spp. é reconhecido como um importante agente etiológico de diarreia em diversos países. O objetivo deste trabalho foi detectar a presença do ADN do protozoário Cryptosporidium spp. em fezes de gatos com histórico de diarreia crônica atendidos no Setor de Medicina Felina do Hospital Veterinário da Universidade Federal Rural do Rio de Janeiro, através da técnica de reação em cadeia da polimerase em tempo real (RT- -PCR). Neste estudo, foram admitidos 100 animais da espécie felina, de diferentes raças, de ambos os sexos e a partir de 8 semanas de idade. Como critério de inclusão, os pacientes deveriam apresentar histórico de diarreia a mais de três semanas, com pouco sucesso de resposta clínica para terapêuticas previamente instituídas. A partir das amostras de fezes obtidas por coleta via lavagem do cólon dos animais e pelo ato de defecação espontânea, foram realizados os métodos do exame direto das fezes, técnica de centrifugo flutuação e PCR em tempo real. Dos 100 gatos que foram selecionados para esta pesquisa, 10% apresentaram infecção por Cryptosporidium spp. A maioria dos animais positivos estavam na faixa etária acima de um ano (70%) e apenas 30% tinha até um ano de idade. Os gatos eram 50% de raça pura e 50% eram animais sem raça definida. Os sinais clínicos apresentados por esses gatos no momento da consulta foram diarreia (60%) e prolapso de reto (40%). Quatro animais apresentaram co- infecções com outros enteropatógenos (40%), um com Giardia sp., um com Toxocara sp., um com Tritrichomonas foetus e outro com Toxocara sp. e Giardia sp. Podemos concluir que a infecção por Cryptosporidium spp. é um importante diagnóstico diferencial em felinos com diarreia crônica, que gatos de qualquer idade e independente de ser de raça pura ou não, podem apresentar a infecção e que a co-infecção com outros enteropatógenos necessita ser investigada em todos casos.Sociedade de Medicina Veterinária do Estado do Rio de Janeiro.2016-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionpeer reviewedAvaliado pelos paresapplication/pdfhttps://rbmv.org/BJVM/article/view/170Brazilian Journal of Veterinary Medicine; Vol. 38 No. Supl.2 (2016); 22-26Revista Brasileira de Medicina Veterinária; v. 38 n. Supl.2 (2016); 22-262527-21790100-2430reponame:Brazilian Journal of Veterinary Medicineinstname:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)instacron:SBMVporhttps://rbmv.org/BJVM/article/view/170/110Carrasco, Lara Patrícia Santosde Oliveira, Renato Leão SáMoreira, Clarissa Martins do RioSantos, Carla Regina Gomes RodriguesCorgozinho, Kátia BarãoSouza, Heloisa Justen Moreirainfo:eu-repo/semantics/openAccess2020-12-23T17:34:13Zoai:ojs.rbmv.org:article/170Revistahttps://rbmv.org/BJVMONGhttps://rbmv.org/BJVM/oaicontato.rbmv@gmail.com2527-21790100-2430opendoar:2020-12-23T17:34:13Brazilian Journal of Veterinary Medicine - Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)false
dc.title.none.fl_str_mv Cryptosporidium diagnosis by qPCR in cats at Rio de Janeiro state, Brazil
Diagnóstico de Cryptosporidium spp. pela técnica de qPCR em gatos no estado do Rio de Janeiro, Brasil
title Cryptosporidium diagnosis by qPCR in cats at Rio de Janeiro state, Brazil
spellingShingle Cryptosporidium diagnosis by qPCR in cats at Rio de Janeiro state, Brazil
Carrasco, Lara Patrícia Santos
Gatos
diarreia
Cryptosporidium spp
Cats
diarrhea
Cryptosporidium spp
title_short Cryptosporidium diagnosis by qPCR in cats at Rio de Janeiro state, Brazil
title_full Cryptosporidium diagnosis by qPCR in cats at Rio de Janeiro state, Brazil
title_fullStr Cryptosporidium diagnosis by qPCR in cats at Rio de Janeiro state, Brazil
title_full_unstemmed Cryptosporidium diagnosis by qPCR in cats at Rio de Janeiro state, Brazil
title_sort Cryptosporidium diagnosis by qPCR in cats at Rio de Janeiro state, Brazil
author Carrasco, Lara Patrícia Santos
author_facet Carrasco, Lara Patrícia Santos
de Oliveira, Renato Leão Sá
Moreira, Clarissa Martins do Rio
Santos, Carla Regina Gomes Rodrigues
Corgozinho, Kátia Barão
Souza, Heloisa Justen Moreira
author_role author
author2 de Oliveira, Renato Leão Sá
Moreira, Clarissa Martins do Rio
Santos, Carla Regina Gomes Rodrigues
Corgozinho, Kátia Barão
Souza, Heloisa Justen Moreira
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Carrasco, Lara Patrícia Santos
de Oliveira, Renato Leão Sá
Moreira, Clarissa Martins do Rio
Santos, Carla Regina Gomes Rodrigues
Corgozinho, Kátia Barão
Souza, Heloisa Justen Moreira
dc.subject.por.fl_str_mv Gatos
diarreia
Cryptosporidium spp
Cats
diarrhea
Cryptosporidium spp
topic Gatos
diarreia
Cryptosporidium spp
Cats
diarrhea
Cryptosporidium spp
description ABSTRACT. Carrasco L.P.S., Oliveira R.L.S., Moreira C.M.R., Santos C.R.G.R., Corgozinho K.B. & Souza H.J.M. [Cryptosporidium diagnosis by qPCR in cats at Rio de Janeiro state, Brazil.] Diagnóstico de Cryptosporidium spp. pela técnica de qPCR em gatos no estado do Rio de Janeiro, Brasil. Revista Brasileira de Medicina Veterinária, 38(Supl.):22-26, 2016. Programa de Pós-Graduação em MedicinaVeteriná- ria, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, BR-465, Km 7, Seropédica, RJ 23890-000, Brasil. E-mail: carrasco.lara@gmail.com Cryptosporidium spp. is recognized as an important etiologic agent of diarrhea in many countries. The aim of this study was to detect the presence of DNA of the parasite Cryptosporidium spp. in feces of cats with history of chronic diarrhea attended in the Feline Medicine Sector of the Veterinary Hospital of the Federal Rural University of Rio de Janeiro, by the polymerase chain reaction technique in real time (RT-PCR). In this study, 100 animals were admitted, of any breed or sex and from 8 weeks of age. As inclusion criteria, patients had to have diarrhea history for more than three weeks, with little success of clinical response to previously established therapies. From the samples obtained by collecting via washing the animal colon and spontaneous defecation, methods of direct examination of the feces, centrifugal flotation technique and real-time PCR were carried out. Of all cats selected for this study, 10% showed infection by Cryptosporidium spp. Most positive animals were aged over one year (70%) and only 30% had up to one year old. Cats were 50% purebred and 50% were domestic short hair cats. The clinical signs presented by these cats at the time of consultation were diarrhea (60%) and prolapsed rectum (40%). Four animals had co-infections with other enteropathogens (40%), such as Giardia, Toxocara sp. or Tritrichomonas fetus alone or combined. We concluded that infection by Cryptosporidium spp. is an important differential diagnosis in cats with chronic diarrhea, in cats of all ages and regardless of whether purebred or not, may have the infection and co-infection with other enteric pathogens needs to be investigated in all cases.
publishDate 2016
dc.date.none.fl_str_mv 2016-11-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
peer reviewed
Avaliado pelos pares
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rbmv.org/BJVM/article/view/170
url https://rbmv.org/BJVM/article/view/170
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://rbmv.org/BJVM/article/view/170/110
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Sociedade de Medicina Veterinária do Estado do Rio de Janeiro.
publisher.none.fl_str_mv Sociedade de Medicina Veterinária do Estado do Rio de Janeiro.
dc.source.none.fl_str_mv Brazilian Journal of Veterinary Medicine; Vol. 38 No. Supl.2 (2016); 22-26
Revista Brasileira de Medicina Veterinária; v. 38 n. Supl.2 (2016); 22-26
2527-2179
0100-2430
reponame:Brazilian Journal of Veterinary Medicine
instname:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)
instacron:SBMV
instname_str Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)
instacron_str SBMV
institution SBMV
reponame_str Brazilian Journal of Veterinary Medicine
collection Brazilian Journal of Veterinary Medicine
repository.name.fl_str_mv Brazilian Journal of Veterinary Medicine - Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)
repository.mail.fl_str_mv contato.rbmv@gmail.com
_version_ 1798313106888196096