COMPARATION OF THE MEAT OF WILD BOAR (Sus scrofa scrofa) IN RELATION TO THE ONE OF OTHER SPECIES IN WHAT IT REFERS TO THE HYDROXYPROLINE
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Brazilian Journal of Veterinary Medicine |
Texto Completo: | https://rbmv.org/BJVM/article/view/774 |
Resumo: | ABSTRACT. Abreu R.L. de, Godoy R.L.O., Santos I.F., Pacheco S. & Souza R.S. [Comparation of the meat of wild boar (Sus scrofa scrofa) in relation to the one of other species in what it refers to the hydroxyproline.] Comparação da carne de javali (Sus scrofa scrofa) em relação à de outras espécies no que tange a hidroxiprolina. Revista Brasileira de Medicina Veterinária, 33(1):58-60, 2011. Departamento de Epidemiologia e Saúde Pública, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, BR 465, km 7, Seropédica, RJ 23890-000, Brasil. E-mail: robsonlopesabreu@uol.com.br Hydroxyproline is exclusive not essential amino acid of the colagen. The objective of this study was comparativily to explain the soft of the meat of wild boar to the one of other species considering itself it hydroxyproline concentration. One studied Longissimus muscles dorsi (LD) and Infraspinatus (IE) of six wild boars. Samples had been collected at three moments (before the entrance of the refrigerating chamber - 12 and 360h) after bleeding and kept cooled. The used standard was trans-4-Hydroxy-L-Proline, Sigma. The procedures of analysis in CLAE (Waters 2695) with fluorescence detector (Waters 474) had been carried through in the Embrapa Agroindústria de Alimentos. The samples had been hydrolyzed during 24h to 110o C in acid clorídrico 6M in blister stamped to the vacuum. After that one moved to volumetric balloon (50mL), was centrifugalized and an aliquot one of 50 μL was kept in desiccator (24h). The samples had been reconstituted (20mM of HCl) and preceded it derivatization with 6- aminoquinolyl-N-succinimidyl carbamate (AQC). After 1 minute of reaction was placed in greenhouse to 55o C during 10 minutes, after which if preceded the chromatographic analysis. The results observed in the present work had been: 261.4 μg.g-1 in muscle “LD”; 343 μg.g-1 in “IE” and 302.2 μg.g-1 in wild boar (general), considering average it of these muscles. The concentration in hydroxyproline in muscle “LD” of bovine is 1030 μg.g-1 of sample; in turtle meat (female, 814 μg.g-1 and males, 728 μg.g-1 of sample). One evidences that: the concentration in muscle “IE” of wild boar was higher in 31.2% in relation to the “LD”; in muscle “LD” of bovine higher in relation to the one of wild boar was 294%. In relation to the concentration of hydroxyproline in turtle one evidenced that: for females he was higher in 169.3%, and for males, in 58.5% when compared with the wild boar meat (general). One concludes that: “LD” of wild boar is softer than “IE” and “LD” of bovine; the wild boar meat (general) is softer than of turtle. |
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COMPARATION OF THE MEAT OF WILD BOAR (Sus scrofa scrofa) IN RELATION TO THE ONE OF OTHER SPECIES IN WHAT IT REFERS TO THE HYDROXYPROLINECOMPARAÇÃO DA CARNE DE JAVALI (Sus scrofa scrofa) EM RELAÇÃO À DE OUTRAS ESPÉCIES NO QUE TANGE A HIDROXIPROLINAHidroxiprolinacromatografia líquida de alta eficiênciacarne de javaliHydroxyprolineliquid chromatography of high efficiencywild boar meatABSTRACT. Abreu R.L. de, Godoy R.L.O., Santos I.F., Pacheco S. & Souza R.S. [Comparation of the meat of wild boar (Sus scrofa scrofa) in relation to the one of other species in what it refers to the hydroxyproline.] Comparação da carne de javali (Sus scrofa scrofa) em relação à de outras espécies no que tange a hidroxiprolina. Revista Brasileira de Medicina Veterinária, 33(1):58-60, 2011. Departamento de Epidemiologia e Saúde Pública, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, BR 465, km 7, Seropédica, RJ 23890-000, Brasil. E-mail: robsonlopesabreu@uol.com.br Hydroxyproline is exclusive not essential amino acid of the colagen. The objective of this study was comparativily to explain the soft of the meat of wild boar to the one of other species considering itself it hydroxyproline concentration. One studied Longissimus muscles dorsi (LD) and Infraspinatus (IE) of six wild boars. Samples had been collected at three moments (before the entrance of the refrigerating chamber - 12 and 360h) after bleeding and kept cooled. The used standard was trans-4-Hydroxy-L-Proline, Sigma. The procedures of analysis in CLAE (Waters 2695) with fluorescence detector (Waters 474) had been carried through in the Embrapa Agroindústria de Alimentos. The samples had been hydrolyzed during 24h to 110o C in acid clorídrico 6M in blister stamped to the vacuum. After that one moved to volumetric balloon (50mL), was centrifugalized and an aliquot one of 50 μL was kept in desiccator (24h). The samples had been reconstituted (20mM of HCl) and preceded it derivatization with 6- aminoquinolyl-N-succinimidyl carbamate (AQC). After 1 minute of reaction was placed in greenhouse to 55o C during 10 minutes, after which if preceded the chromatographic analysis. The results observed in the present work had been: 261.4 μg.g-1 in muscle “LD”; 343 μg.g-1 in “IE” and 302.2 μg.g-1 in wild boar (general), considering average it of these muscles. The concentration in hydroxyproline in muscle “LD” of bovine is 1030 μg.g-1 of sample; in turtle meat (female, 814 μg.g-1 and males, 728 μg.g-1 of sample). One evidences that: the concentration in muscle “IE” of wild boar was higher in 31.2% in relation to the “LD”; in muscle “LD” of bovine higher in relation to the one of wild boar was 294%. In relation to the concentration of hydroxyproline in turtle one evidenced that: for females he was higher in 169.3%, and for males, in 58.5% when compared with the wild boar meat (general). One concludes that: “LD” of wild boar is softer than “IE” and “LD” of bovine; the wild boar meat (general) is softer than of turtle.Hidroxiprolina é aminoácido não essencial exclusivo do colágeno. O objetivo deste estudo foi explicar a maciez da carne de javali comparativamente à de outras espécies considerando-se a concentração de hidroxiprolina. Estudaram-se músculos Longissimus dorsi (LD) e Infraspinatus (IE) de seis javalis. Amostras foram recolhidas em três momentos (antes da entrada da câmara frigorífica - 12 e 360h) após sangria e mantidas resfriadas. O padrão utilizado foi trans-4-Hydroxy-L-Proline, Sigma. Os procedimentos de análise em CLAE (Waters 2695) com detector de fluorescência (Waters 474) foram realizados na Embrapa Agroindústria de Alimentos. As amostras foram hidrolisadas durante 24h à 110o C em ácido clorídrico 6M em ampola selada à vácuo. Em seguida transferiuse para balão volumétrico (50mL), centrifugou-se e uma alíquota de 50μL foi mantida em dessecador (24h). As amostras foram reconstituídas (20mM de HCl) e procedeu-se a derivatização com 6-aminoquinolyl-N-succinimidyl carbamate (AQC). Após 1 minuto de reação colocou-se em estufa à 55°C durante 10 minutos, após os quais se procedeu a análise cromatográfica. Os resultados observados no presente trabalho foram: 261,4μg.g-1 no músculo “LD”, 343μg.g-1 no “IE”, e 302,2μg.g-1 em javali (geral), considerando-se a média destes músculos. A concentração em hidroxiprolina no músculo “LD” de bovino é 1030μg.g-1 de amostra; em carne de tartaruga (fêmeas, 814μg.g-1 e machos, 728μg.g-1 de amostra). Constata-se que: a concentração no músculo “IE” de javali foi superior em 31,2% em relação ao “LD”; no músculo “LD” de bovino foi 294% superior em relação ao de javali. Em relação à concentração de hidroxiprolina em tartaruga constatou-se que: para fêmeas foi superior em 169,3%, e para machos, em 58,5% quando comparados à carne de javali (geral).Conclui-se que: “LD” de javali é mais macio que o “IE” e “LD” de bovino; a carne de javali (geral) é mais macia que a de tartaruga.Sociedade de Medicina Veterinária do Estado do Rio de Janeiro.2011-03-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionpeer reviewedAvaliado pelos paresapplication/pdfhttps://rbmv.org/BJVM/article/view/774Brazilian Journal of Veterinary Medicine; Vol. 33 No. 1 (2011); 58-60Revista Brasileira de Medicina Veterinária; v. 33 n. 1 (2011); 58-602527-21790100-2430reponame:Brazilian Journal of Veterinary Medicineinstname:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)instacron:SBMVporhttps://rbmv.org/BJVM/article/view/774/633de Abreu, Robson LopesGodóy, Ronoel Luiz de Oliveirados Santos, Iacir FranciscoPacheco, Sidneyde Souza, Rafael Santosinfo:eu-repo/semantics/openAccess2020-12-23T17:27:55Zoai:ojs.rbmv.org:article/774Revistahttps://rbmv.org/BJVMONGhttps://rbmv.org/BJVM/oaicontato.rbmv@gmail.com2527-21790100-2430opendoar:2020-12-23T17:27:55Brazilian Journal of Veterinary Medicine - Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)false |
dc.title.none.fl_str_mv |
COMPARATION OF THE MEAT OF WILD BOAR (Sus scrofa scrofa) IN RELATION TO THE ONE OF OTHER SPECIES IN WHAT IT REFERS TO THE HYDROXYPROLINE COMPARAÇÃO DA CARNE DE JAVALI (Sus scrofa scrofa) EM RELAÇÃO À DE OUTRAS ESPÉCIES NO QUE TANGE A HIDROXIPROLINA |
title |
COMPARATION OF THE MEAT OF WILD BOAR (Sus scrofa scrofa) IN RELATION TO THE ONE OF OTHER SPECIES IN WHAT IT REFERS TO THE HYDROXYPROLINE |
spellingShingle |
COMPARATION OF THE MEAT OF WILD BOAR (Sus scrofa scrofa) IN RELATION TO THE ONE OF OTHER SPECIES IN WHAT IT REFERS TO THE HYDROXYPROLINE de Abreu, Robson Lopes Hidroxiprolina cromatografia líquida de alta eficiência carne de javali Hydroxyproline liquid chromatography of high efficiency wild boar meat |
title_short |
COMPARATION OF THE MEAT OF WILD BOAR (Sus scrofa scrofa) IN RELATION TO THE ONE OF OTHER SPECIES IN WHAT IT REFERS TO THE HYDROXYPROLINE |
title_full |
COMPARATION OF THE MEAT OF WILD BOAR (Sus scrofa scrofa) IN RELATION TO THE ONE OF OTHER SPECIES IN WHAT IT REFERS TO THE HYDROXYPROLINE |
title_fullStr |
COMPARATION OF THE MEAT OF WILD BOAR (Sus scrofa scrofa) IN RELATION TO THE ONE OF OTHER SPECIES IN WHAT IT REFERS TO THE HYDROXYPROLINE |
title_full_unstemmed |
COMPARATION OF THE MEAT OF WILD BOAR (Sus scrofa scrofa) IN RELATION TO THE ONE OF OTHER SPECIES IN WHAT IT REFERS TO THE HYDROXYPROLINE |
title_sort |
COMPARATION OF THE MEAT OF WILD BOAR (Sus scrofa scrofa) IN RELATION TO THE ONE OF OTHER SPECIES IN WHAT IT REFERS TO THE HYDROXYPROLINE |
author |
de Abreu, Robson Lopes |
author_facet |
de Abreu, Robson Lopes Godóy, Ronoel Luiz de Oliveira dos Santos, Iacir Francisco Pacheco, Sidney de Souza, Rafael Santos |
author_role |
author |
author2 |
Godóy, Ronoel Luiz de Oliveira dos Santos, Iacir Francisco Pacheco, Sidney de Souza, Rafael Santos |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
de Abreu, Robson Lopes Godóy, Ronoel Luiz de Oliveira dos Santos, Iacir Francisco Pacheco, Sidney de Souza, Rafael Santos |
dc.subject.por.fl_str_mv |
Hidroxiprolina cromatografia líquida de alta eficiência carne de javali Hydroxyproline liquid chromatography of high efficiency wild boar meat |
topic |
Hidroxiprolina cromatografia líquida de alta eficiência carne de javali Hydroxyproline liquid chromatography of high efficiency wild boar meat |
description |
ABSTRACT. Abreu R.L. de, Godoy R.L.O., Santos I.F., Pacheco S. & Souza R.S. [Comparation of the meat of wild boar (Sus scrofa scrofa) in relation to the one of other species in what it refers to the hydroxyproline.] Comparação da carne de javali (Sus scrofa scrofa) em relação à de outras espécies no que tange a hidroxiprolina. Revista Brasileira de Medicina Veterinária, 33(1):58-60, 2011. Departamento de Epidemiologia e Saúde Pública, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, BR 465, km 7, Seropédica, RJ 23890-000, Brasil. E-mail: robsonlopesabreu@uol.com.br Hydroxyproline is exclusive not essential amino acid of the colagen. The objective of this study was comparativily to explain the soft of the meat of wild boar to the one of other species considering itself it hydroxyproline concentration. One studied Longissimus muscles dorsi (LD) and Infraspinatus (IE) of six wild boars. Samples had been collected at three moments (before the entrance of the refrigerating chamber - 12 and 360h) after bleeding and kept cooled. The used standard was trans-4-Hydroxy-L-Proline, Sigma. The procedures of analysis in CLAE (Waters 2695) with fluorescence detector (Waters 474) had been carried through in the Embrapa Agroindústria de Alimentos. The samples had been hydrolyzed during 24h to 110o C in acid clorídrico 6M in blister stamped to the vacuum. After that one moved to volumetric balloon (50mL), was centrifugalized and an aliquot one of 50 μL was kept in desiccator (24h). The samples had been reconstituted (20mM of HCl) and preceded it derivatization with 6- aminoquinolyl-N-succinimidyl carbamate (AQC). After 1 minute of reaction was placed in greenhouse to 55o C during 10 minutes, after which if preceded the chromatographic analysis. The results observed in the present work had been: 261.4 μg.g-1 in muscle “LD”; 343 μg.g-1 in “IE” and 302.2 μg.g-1 in wild boar (general), considering average it of these muscles. The concentration in hydroxyproline in muscle “LD” of bovine is 1030 μg.g-1 of sample; in turtle meat (female, 814 μg.g-1 and males, 728 μg.g-1 of sample). One evidences that: the concentration in muscle “IE” of wild boar was higher in 31.2% in relation to the “LD”; in muscle “LD” of bovine higher in relation to the one of wild boar was 294%. In relation to the concentration of hydroxyproline in turtle one evidenced that: for females he was higher in 169.3%, and for males, in 58.5% when compared with the wild boar meat (general). One concludes that: “LD” of wild boar is softer than “IE” and “LD” of bovine; the wild boar meat (general) is softer than of turtle. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-03-30 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion peer reviewed Avaliado pelos pares |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rbmv.org/BJVM/article/view/774 |
url |
https://rbmv.org/BJVM/article/view/774 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://rbmv.org/BJVM/article/view/774/633 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade de Medicina Veterinária do Estado do Rio de Janeiro. |
publisher.none.fl_str_mv |
Sociedade de Medicina Veterinária do Estado do Rio de Janeiro. |
dc.source.none.fl_str_mv |
Brazilian Journal of Veterinary Medicine; Vol. 33 No. 1 (2011); 58-60 Revista Brasileira de Medicina Veterinária; v. 33 n. 1 (2011); 58-60 2527-2179 0100-2430 reponame:Brazilian Journal of Veterinary Medicine instname:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ) instacron:SBMV |
instname_str |
Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ) |
instacron_str |
SBMV |
institution |
SBMV |
reponame_str |
Brazilian Journal of Veterinary Medicine |
collection |
Brazilian Journal of Veterinary Medicine |
repository.name.fl_str_mv |
Brazilian Journal of Veterinary Medicine - Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ) |
repository.mail.fl_str_mv |
contato.rbmv@gmail.com |
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1798313109505441792 |