Spermatic Viability of Cryopreserved Semen of Piau swine breed analyzed by Thermo Resistant Test
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Brazilian Journal of Veterinary Medicine |
Texto Completo: | https://rbmv.org/BJVM/article/view/512 |
Resumo: | ABSTRACT. Barros M.H.C., Shiomi H.H., Amorim L.S., Guimarães S.E.F., Lopes P.S., Siqueira J.B., Pinho R.O. & Guimarães J.D. [Spermatic Viability of Cryopreserved Semen of Piau swine breed analyzed by Thermo Resistant Test.] Viabilidade espermática de sêmen congelado de suínos da raça Piau avaliada pelo Teste de Termorresistência. Revista Brasileira de Medicina Veterinária 36(2):131-136, 2014. Departamento de Veterinária, Universidade Federal de Viçosa, Av. Peter Henry Rolfs, s/n, Viçosa, MG 36571-000, Brasil. E-mail: hugoshiomi@hotmail.com The objective of this study was to verify three protocols of semen cryopreservation on spermatic viability after thawing from Piau swine breed (Sus scrofa), by thermo resistant test (TTR). Twenty two ejaculates from 5 Piau mature boars were collected by glove hand technique. To freezing, the ejaculates was split and submitted to three protocols (P): (P1) freezing method proposed by Furst et al. (2005), altered by diluent media, (P2) freezing method proposed by Furst et al. (2005), altered by cooled curve; and (P3) freezing method proposed by Ohata et al. (2001). After thawing, semen was submitted to TTR, been incubated at 37o C by 2 hours, motility sperm (MOT) and vigor (VIG) was analyzed at 30 minutes of interval. MOT and VIG after thawing was 20.9±12.4, 29.5±10.9 and 49.5±12.1%; 2.5±0.5, 2.9±0.4 and 3.4±0.4, respectively to P1, P2 and P3. The TTR results show gradually decrease of motility and vigor along 2 hours of test procedure utilized, with best average to protocol 3 at all time of analyze. The protocol 3 tested by Piau boars shows highest values in cellular semen cryopreservation. |
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Spermatic Viability of Cryopreserved Semen of Piau swine breed analyzed by Thermo Resistant TestViabilidade espermática de sêmen congelado de suínos da raça Piau avaliada pelo teste de termorresistênciaCriopreservação de sêmenSus scrofatestes complementaresComplementary testssemen cryopreservationSus scrofaABSTRACT. Barros M.H.C., Shiomi H.H., Amorim L.S., Guimarães S.E.F., Lopes P.S., Siqueira J.B., Pinho R.O. & Guimarães J.D. [Spermatic Viability of Cryopreserved Semen of Piau swine breed analyzed by Thermo Resistant Test.] Viabilidade espermática de sêmen congelado de suínos da raça Piau avaliada pelo Teste de Termorresistência. Revista Brasileira de Medicina Veterinária 36(2):131-136, 2014. Departamento de Veterinária, Universidade Federal de Viçosa, Av. Peter Henry Rolfs, s/n, Viçosa, MG 36571-000, Brasil. E-mail: hugoshiomi@hotmail.com The objective of this study was to verify three protocols of semen cryopreservation on spermatic viability after thawing from Piau swine breed (Sus scrofa), by thermo resistant test (TTR). Twenty two ejaculates from 5 Piau mature boars were collected by glove hand technique. To freezing, the ejaculates was split and submitted to three protocols (P): (P1) freezing method proposed by Furst et al. (2005), altered by diluent media, (P2) freezing method proposed by Furst et al. (2005), altered by cooled curve; and (P3) freezing method proposed by Ohata et al. (2001). After thawing, semen was submitted to TTR, been incubated at 37o C by 2 hours, motility sperm (MOT) and vigor (VIG) was analyzed at 30 minutes of interval. MOT and VIG after thawing was 20.9±12.4, 29.5±10.9 and 49.5±12.1%; 2.5±0.5, 2.9±0.4 and 3.4±0.4, respectively to P1, P2 and P3. The TTR results show gradually decrease of motility and vigor along 2 hours of test procedure utilized, with best average to protocol 3 at all time of analyze. The protocol 3 tested by Piau boars shows highest values in cellular semen cryopreservation.O objetivo do presente trabalho foi verificar o efeito de três protocolos de criopreservação de sêmen (P1, P2 e P3) sobre a viabilidade espermática pós-descongelamento de suínos da raça Piau, avaliados por meio do teste de termorresistência (TTR). Para o congelamento, os ejaculados foram fracionados e submetidos aos seguintes tratamentos: o P1 utilizou o método de congelamento preconizado por Fürst et al. (2005), modificado quanto aos meios diluentes; o P2 utilizou o método preconizado por Fürst et al. (2005), modificado quanto à curva de resfriamento; e o P3 utilizou o método preconizado por Ohata et al. (2001). Após o descongelamento o sêmen foi submetido ao TTR (37°C por 2 horas), procedendo-se avaliações de motilidade (MOT) e vigor espermáticos (VIG) a cada 30 minutos. As médias registradas para MOT e VIG imediatamente após o descongelamento foram de 20,9±12,4; 29,5±10,9 e 49,5±12,1%; e de 2,5±0,5; 2,9±0,4 e 3,4±0,4, respectivamente para os protocolos 1, 2 e 3. Os resultados do TTR evidenciam queda gradual dos parâmetros de MOT e VIG ao longo de 2 horas de duração nos três protocolos utilizados, com melhores médias para o protocolo 3, demonstram a superioridade do mesmo na criopreservação das células espermáticas dos animais do presente estudoSociedade de Medicina Veterinária do Estado do Rio de Janeiro.2014-06-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionpeer reviewedAvaliado pelos paresapplication/pdfhttps://rbmv.org/BJVM/article/view/512Brazilian Journal of Veterinary Medicine; Vol. 36 No. 2 (2014); 131-136Revista Brasileira de Medicina Veterinária; v. 36 n. 2 (2014); 131-1362527-21790100-2430reponame:Brazilian Journal of Veterinary Medicineinstname:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)instacron:SBMVporhttps://rbmv.org/BJVM/article/view/512/389Barros, Maurício Hoshino da CostaShiomi, Hugo HidekiAmorim, Lincoln da SilvaGuimarães, Simone Eliza FacioniLopes, Paulo SávioSiqueira, Jeanne BrochPinho, Rogério OliveiraGuimarães, José Domingosinfo:eu-repo/semantics/openAccess2020-12-23T17:32:15Zoai:ojs.rbmv.org:article/512Revistahttps://rbmv.org/BJVMONGhttps://rbmv.org/BJVM/oaicontato.rbmv@gmail.com2527-21790100-2430opendoar:2020-12-23T17:32:15Brazilian Journal of Veterinary Medicine - Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)false |
dc.title.none.fl_str_mv |
Spermatic Viability of Cryopreserved Semen of Piau swine breed analyzed by Thermo Resistant Test Viabilidade espermática de sêmen congelado de suínos da raça Piau avaliada pelo teste de termorresistência |
title |
Spermatic Viability of Cryopreserved Semen of Piau swine breed analyzed by Thermo Resistant Test |
spellingShingle |
Spermatic Viability of Cryopreserved Semen of Piau swine breed analyzed by Thermo Resistant Test Barros, Maurício Hoshino da Costa Criopreservação de sêmen Sus scrofa testes complementares Complementary tests semen cryopreservation Sus scrofa |
title_short |
Spermatic Viability of Cryopreserved Semen of Piau swine breed analyzed by Thermo Resistant Test |
title_full |
Spermatic Viability of Cryopreserved Semen of Piau swine breed analyzed by Thermo Resistant Test |
title_fullStr |
Spermatic Viability of Cryopreserved Semen of Piau swine breed analyzed by Thermo Resistant Test |
title_full_unstemmed |
Spermatic Viability of Cryopreserved Semen of Piau swine breed analyzed by Thermo Resistant Test |
title_sort |
Spermatic Viability of Cryopreserved Semen of Piau swine breed analyzed by Thermo Resistant Test |
author |
Barros, Maurício Hoshino da Costa |
author_facet |
Barros, Maurício Hoshino da Costa Shiomi, Hugo Hideki Amorim, Lincoln da Silva Guimarães, Simone Eliza Facioni Lopes, Paulo Sávio Siqueira, Jeanne Broch Pinho, Rogério Oliveira Guimarães, José Domingos |
author_role |
author |
author2 |
Shiomi, Hugo Hideki Amorim, Lincoln da Silva Guimarães, Simone Eliza Facioni Lopes, Paulo Sávio Siqueira, Jeanne Broch Pinho, Rogério Oliveira Guimarães, José Domingos |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Barros, Maurício Hoshino da Costa Shiomi, Hugo Hideki Amorim, Lincoln da Silva Guimarães, Simone Eliza Facioni Lopes, Paulo Sávio Siqueira, Jeanne Broch Pinho, Rogério Oliveira Guimarães, José Domingos |
dc.subject.por.fl_str_mv |
Criopreservação de sêmen Sus scrofa testes complementares Complementary tests semen cryopreservation Sus scrofa |
topic |
Criopreservação de sêmen Sus scrofa testes complementares Complementary tests semen cryopreservation Sus scrofa |
description |
ABSTRACT. Barros M.H.C., Shiomi H.H., Amorim L.S., Guimarães S.E.F., Lopes P.S., Siqueira J.B., Pinho R.O. & Guimarães J.D. [Spermatic Viability of Cryopreserved Semen of Piau swine breed analyzed by Thermo Resistant Test.] Viabilidade espermática de sêmen congelado de suínos da raça Piau avaliada pelo Teste de Termorresistência. Revista Brasileira de Medicina Veterinária 36(2):131-136, 2014. Departamento de Veterinária, Universidade Federal de Viçosa, Av. Peter Henry Rolfs, s/n, Viçosa, MG 36571-000, Brasil. E-mail: hugoshiomi@hotmail.com The objective of this study was to verify three protocols of semen cryopreservation on spermatic viability after thawing from Piau swine breed (Sus scrofa), by thermo resistant test (TTR). Twenty two ejaculates from 5 Piau mature boars were collected by glove hand technique. To freezing, the ejaculates was split and submitted to three protocols (P): (P1) freezing method proposed by Furst et al. (2005), altered by diluent media, (P2) freezing method proposed by Furst et al. (2005), altered by cooled curve; and (P3) freezing method proposed by Ohata et al. (2001). After thawing, semen was submitted to TTR, been incubated at 37o C by 2 hours, motility sperm (MOT) and vigor (VIG) was analyzed at 30 minutes of interval. MOT and VIG after thawing was 20.9±12.4, 29.5±10.9 and 49.5±12.1%; 2.5±0.5, 2.9±0.4 and 3.4±0.4, respectively to P1, P2 and P3. The TTR results show gradually decrease of motility and vigor along 2 hours of test procedure utilized, with best average to protocol 3 at all time of analyze. The protocol 3 tested by Piau boars shows highest values in cellular semen cryopreservation. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-06-06 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion peer reviewed Avaliado pelos pares |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rbmv.org/BJVM/article/view/512 |
url |
https://rbmv.org/BJVM/article/view/512 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://rbmv.org/BJVM/article/view/512/389 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade de Medicina Veterinária do Estado do Rio de Janeiro. |
publisher.none.fl_str_mv |
Sociedade de Medicina Veterinária do Estado do Rio de Janeiro. |
dc.source.none.fl_str_mv |
Brazilian Journal of Veterinary Medicine; Vol. 36 No. 2 (2014); 131-136 Revista Brasileira de Medicina Veterinária; v. 36 n. 2 (2014); 131-136 2527-2179 0100-2430 reponame:Brazilian Journal of Veterinary Medicine instname:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ) instacron:SBMV |
instname_str |
Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ) |
instacron_str |
SBMV |
institution |
SBMV |
reponame_str |
Brazilian Journal of Veterinary Medicine |
collection |
Brazilian Journal of Veterinary Medicine |
repository.name.fl_str_mv |
Brazilian Journal of Veterinary Medicine - Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ) |
repository.mail.fl_str_mv |
contato.rbmv@gmail.com |
_version_ |
1798313108205207552 |