Flotation solution influence on the quantification of Ascaris suum eggs in pig feces using McMaster technique

Detalhes bibliográficos
Autor(a) principal: Fausto, Mariana Costa
Data de Publicação: 2021
Outros Autores: Saraiva, Luís Henrique Gouvêa, Fausto, Guilherme Costa, de Carvalho, Lorendane Millena, Valente, Fabrício Luciani, Valadão, Marisa Caixeta, Campos, Artur Kanadani, de Araújo, Jackson Victor
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Veterinary Medicine
Texto Completo: https://rbmv.org/BJVM/article/view/1198
Resumo: Parasitic diseases are responsible for economic losses in the pig production industry. It is necessary to monitor the parasite load through stool ova and parasite analysis to avoid losses to the producer. Among analytical tests, the McMaster technique is the most widely used method; however, several variations of this method have been described, and, therefore, there is a need for standardization. The aim of this study was to evaluate the use of different saturated solutions prepared from four different salts (NaCl, MgSO4, NaNO3, ZnSO4), a sugar solution, and Polysorbate Tween 80, to perform the McMaster technique. Pig feces containing Ascaris suum eggs were homogenized in Griffin beakers and divided into 2g aliquots for the exams with different solutions. All solutions evaluated were able to float A. suum eggs. However, eggs per gram of feces (EPG) values using the sodium nitrate solution were higher (p < 0.05) than for the other solutions. Magnesium sulfate and sodium chloride solutions provided intermediate values for EPG. Lower values were obtained with zinc sulfate and sugar solutions. Polysorbate Tween 80 was added to the sodium nitrate solution at a concentration of 0.2%, and this proved to be more efficient (p < 0.05) in flotation of the eggs.
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spelling Flotation solution influence on the quantification of Ascaris suum eggs in pig feces using McMaster techniqueInfluência da solução de flutuação na quantificação de ovos de Ascaris suum em fezes de suínos utilizando a técnica de McMasterparasitological techniques, sodium nitrate, Tween 80.Parasitic diseases are responsible for economic losses in the pig production industry. It is necessary to monitor the parasite load through stool ova and parasite analysis to avoid losses to the producer. Among analytical tests, the McMaster technique is the most widely used method; however, several variations of this method have been described, and, therefore, there is a need for standardization. The aim of this study was to evaluate the use of different saturated solutions prepared from four different salts (NaCl, MgSO4, NaNO3, ZnSO4), a sugar solution, and Polysorbate Tween 80, to perform the McMaster technique. Pig feces containing Ascaris suum eggs were homogenized in Griffin beakers and divided into 2g aliquots for the exams with different solutions. All solutions evaluated were able to float A. suum eggs. However, eggs per gram of feces (EPG) values using the sodium nitrate solution were higher (p < 0.05) than for the other solutions. Magnesium sulfate and sodium chloride solutions provided intermediate values for EPG. Lower values were obtained with zinc sulfate and sugar solutions. Polysorbate Tween 80 was added to the sodium nitrate solution at a concentration of 0.2%, and this proved to be more efficient (p < 0.05) in flotation of the eggs.As doenças parasitárias são responsáveis por perdas econômicas na indústria suinícola. É necessário monitorar a carga parasitária por meio de análises de ovos de parasitos nas fezes, para evitar perdas ao produtor. Dentre os testes analíticos, a técnica de McMaster é o método mais utilizado; entretanto, diversas variações desse método foram descritas e, portanto, há necessidade de padronização. O objetivo deste estudo foi avaliar a utilização de diferentes soluções saturadas preparadas a partir de quatro diferentes sais (NaCl, MgSO4, NaNO3, ZnSO4), uma solução de açúcar e Polissorbato Tween 80, para a realização da técnica de McMaster. Fezes de suínos contendo ovos de Ascaris suum foram homogeneizadas em béqueres e divididas em alíquotas de 2g para os exames com diferentes soluções. Todas as soluções avaliadas foram capazes de flutuar ovos de A. suum. No entanto, os valores de ovos por grama de fezes (OPG) com a solução de nitrato de sódio foram maiores (p <0,05) do que com as demais soluções. As soluções de sulfato de magnésio e cloreto de sódio forneceram valores intermediários para OPG. Valores mais baixos de OPG foram obtidos com soluções de sulfato de zinco e açúcar. O polissorbato Tween 80 foi adicionado à solução de nitrato de sódio na concentração de 0,2%, e isso aumentou a eficiência (p <0,05) na flutuação dos ovos.Sociedade de Medicina Veterinária do Estado do Rio de Janeiro.2021-10-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionpeer reviewedAvaliado pelos paresapplication/pdftext/xmlhttps://rbmv.org/BJVM/article/view/119810.29374/2527-2179.bjvm002221Brazilian Journal of Veterinary Medicine; Vol. 43 No. 1 (2021); e002221Revista Brasileira de Medicina Veterinária; v. 43 n. 1 (2021); e0022212527-21790100-2430reponame:Brazilian Journal of Veterinary Medicineinstname:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)instacron:SBMVenghttps://rbmv.org/BJVM/article/view/1198/1100https://rbmv.org/BJVM/article/view/1198/1213Copyright (c) 2021 Mariana Costa Fausto, Luís Henrique Gouvêa Saraiva, Guilherme Costa Fausto, Lorendane Millena de Carvalho, Fabrício Luciani Valente, Marisa Caixeta Valadão, Artur Kanadani Campos, Jackson Victor de Araújohttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessFausto, Mariana CostaSaraiva, Luís Henrique GouvêaFausto, Guilherme Costade Carvalho, Lorendane MillenaValente, Fabrício LucianiValadão, Marisa CaixetaCampos, Artur Kanadanide Araújo, Jackson Victor2022-06-26T22:16:26Zoai:ojs.rbmv.org:article/1198Revistahttps://rbmv.org/BJVMONGhttps://rbmv.org/BJVM/oaicontato.rbmv@gmail.com2527-21790100-2430opendoar:2022-06-26T22:16:26Brazilian Journal of Veterinary Medicine - Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)false
dc.title.none.fl_str_mv Flotation solution influence on the quantification of Ascaris suum eggs in pig feces using McMaster technique
Influência da solução de flutuação na quantificação de ovos de Ascaris suum em fezes de suínos utilizando a técnica de McMaster
title Flotation solution influence on the quantification of Ascaris suum eggs in pig feces using McMaster technique
spellingShingle Flotation solution influence on the quantification of Ascaris suum eggs in pig feces using McMaster technique
Fausto, Mariana Costa
parasitological techniques, sodium nitrate, Tween 80.
title_short Flotation solution influence on the quantification of Ascaris suum eggs in pig feces using McMaster technique
title_full Flotation solution influence on the quantification of Ascaris suum eggs in pig feces using McMaster technique
title_fullStr Flotation solution influence on the quantification of Ascaris suum eggs in pig feces using McMaster technique
title_full_unstemmed Flotation solution influence on the quantification of Ascaris suum eggs in pig feces using McMaster technique
title_sort Flotation solution influence on the quantification of Ascaris suum eggs in pig feces using McMaster technique
author Fausto, Mariana Costa
author_facet Fausto, Mariana Costa
Saraiva, Luís Henrique Gouvêa
Fausto, Guilherme Costa
de Carvalho, Lorendane Millena
Valente, Fabrício Luciani
Valadão, Marisa Caixeta
Campos, Artur Kanadani
de Araújo, Jackson Victor
author_role author
author2 Saraiva, Luís Henrique Gouvêa
Fausto, Guilherme Costa
de Carvalho, Lorendane Millena
Valente, Fabrício Luciani
Valadão, Marisa Caixeta
Campos, Artur Kanadani
de Araújo, Jackson Victor
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Fausto, Mariana Costa
Saraiva, Luís Henrique Gouvêa
Fausto, Guilherme Costa
de Carvalho, Lorendane Millena
Valente, Fabrício Luciani
Valadão, Marisa Caixeta
Campos, Artur Kanadani
de Araújo, Jackson Victor
dc.subject.por.fl_str_mv parasitological techniques, sodium nitrate, Tween 80.
topic parasitological techniques, sodium nitrate, Tween 80.
description Parasitic diseases are responsible for economic losses in the pig production industry. It is necessary to monitor the parasite load through stool ova and parasite analysis to avoid losses to the producer. Among analytical tests, the McMaster technique is the most widely used method; however, several variations of this method have been described, and, therefore, there is a need for standardization. The aim of this study was to evaluate the use of different saturated solutions prepared from four different salts (NaCl, MgSO4, NaNO3, ZnSO4), a sugar solution, and Polysorbate Tween 80, to perform the McMaster technique. Pig feces containing Ascaris suum eggs were homogenized in Griffin beakers and divided into 2g aliquots for the exams with different solutions. All solutions evaluated were able to float A. suum eggs. However, eggs per gram of feces (EPG) values using the sodium nitrate solution were higher (p < 0.05) than for the other solutions. Magnesium sulfate and sodium chloride solutions provided intermediate values for EPG. Lower values were obtained with zinc sulfate and sugar solutions. Polysorbate Tween 80 was added to the sodium nitrate solution at a concentration of 0.2%, and this proved to be more efficient (p < 0.05) in flotation of the eggs.
publishDate 2021
dc.date.none.fl_str_mv 2021-10-07
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
peer reviewed
Avaliado pelos pares
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rbmv.org/BJVM/article/view/1198
10.29374/2527-2179.bjvm002221
url https://rbmv.org/BJVM/article/view/1198
identifier_str_mv 10.29374/2527-2179.bjvm002221
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://rbmv.org/BJVM/article/view/1198/1100
https://rbmv.org/BJVM/article/view/1198/1213
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
text/xml
dc.publisher.none.fl_str_mv Sociedade de Medicina Veterinária do Estado do Rio de Janeiro.
publisher.none.fl_str_mv Sociedade de Medicina Veterinária do Estado do Rio de Janeiro.
dc.source.none.fl_str_mv Brazilian Journal of Veterinary Medicine; Vol. 43 No. 1 (2021); e002221
Revista Brasileira de Medicina Veterinária; v. 43 n. 1 (2021); e002221
2527-2179
0100-2430
reponame:Brazilian Journal of Veterinary Medicine
instname:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)
instacron:SBMV
instname_str Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)
instacron_str SBMV
institution SBMV
reponame_str Brazilian Journal of Veterinary Medicine
collection Brazilian Journal of Veterinary Medicine
repository.name.fl_str_mv Brazilian Journal of Veterinary Medicine - Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)
repository.mail.fl_str_mv contato.rbmv@gmail.com
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