Flotation solution influence on the quantification of Ascaris suum eggs in pig feces using McMaster technique
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Veterinary Medicine |
Texto Completo: | https://rbmv.org/BJVM/article/view/1198 |
Resumo: | Parasitic diseases are responsible for economic losses in the pig production industry. It is necessary to monitor the parasite load through stool ova and parasite analysis to avoid losses to the producer. Among analytical tests, the McMaster technique is the most widely used method; however, several variations of this method have been described, and, therefore, there is a need for standardization. The aim of this study was to evaluate the use of different saturated solutions prepared from four different salts (NaCl, MgSO4, NaNO3, ZnSO4), a sugar solution, and Polysorbate Tween 80, to perform the McMaster technique. Pig feces containing Ascaris suum eggs were homogenized in Griffin beakers and divided into 2g aliquots for the exams with different solutions. All solutions evaluated were able to float A. suum eggs. However, eggs per gram of feces (EPG) values using the sodium nitrate solution were higher (p < 0.05) than for the other solutions. Magnesium sulfate and sodium chloride solutions provided intermediate values for EPG. Lower values were obtained with zinc sulfate and sugar solutions. Polysorbate Tween 80 was added to the sodium nitrate solution at a concentration of 0.2%, and this proved to be more efficient (p < 0.05) in flotation of the eggs. |
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Flotation solution influence on the quantification of Ascaris suum eggs in pig feces using McMaster techniqueInfluência da solução de flutuação na quantificação de ovos de Ascaris suum em fezes de suínos utilizando a técnica de McMasterparasitological techniques, sodium nitrate, Tween 80.Parasitic diseases are responsible for economic losses in the pig production industry. It is necessary to monitor the parasite load through stool ova and parasite analysis to avoid losses to the producer. Among analytical tests, the McMaster technique is the most widely used method; however, several variations of this method have been described, and, therefore, there is a need for standardization. The aim of this study was to evaluate the use of different saturated solutions prepared from four different salts (NaCl, MgSO4, NaNO3, ZnSO4), a sugar solution, and Polysorbate Tween 80, to perform the McMaster technique. Pig feces containing Ascaris suum eggs were homogenized in Griffin beakers and divided into 2g aliquots for the exams with different solutions. All solutions evaluated were able to float A. suum eggs. However, eggs per gram of feces (EPG) values using the sodium nitrate solution were higher (p < 0.05) than for the other solutions. Magnesium sulfate and sodium chloride solutions provided intermediate values for EPG. Lower values were obtained with zinc sulfate and sugar solutions. Polysorbate Tween 80 was added to the sodium nitrate solution at a concentration of 0.2%, and this proved to be more efficient (p < 0.05) in flotation of the eggs.As doenças parasitárias são responsáveis por perdas econômicas na indústria suinícola. É necessário monitorar a carga parasitária por meio de análises de ovos de parasitos nas fezes, para evitar perdas ao produtor. Dentre os testes analíticos, a técnica de McMaster é o método mais utilizado; entretanto, diversas variações desse método foram descritas e, portanto, há necessidade de padronização. O objetivo deste estudo foi avaliar a utilização de diferentes soluções saturadas preparadas a partir de quatro diferentes sais (NaCl, MgSO4, NaNO3, ZnSO4), uma solução de açúcar e Polissorbato Tween 80, para a realização da técnica de McMaster. Fezes de suínos contendo ovos de Ascaris suum foram homogeneizadas em béqueres e divididas em alíquotas de 2g para os exames com diferentes soluções. Todas as soluções avaliadas foram capazes de flutuar ovos de A. suum. No entanto, os valores de ovos por grama de fezes (OPG) com a solução de nitrato de sódio foram maiores (p <0,05) do que com as demais soluções. As soluções de sulfato de magnésio e cloreto de sódio forneceram valores intermediários para OPG. Valores mais baixos de OPG foram obtidos com soluções de sulfato de zinco e açúcar. O polissorbato Tween 80 foi adicionado à solução de nitrato de sódio na concentração de 0,2%, e isso aumentou a eficiência (p <0,05) na flutuação dos ovos.Sociedade de Medicina Veterinária do Estado do Rio de Janeiro.2021-10-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionpeer reviewedAvaliado pelos paresapplication/pdftext/xmlhttps://rbmv.org/BJVM/article/view/119810.29374/2527-2179.bjvm002221Brazilian Journal of Veterinary Medicine; Vol. 43 No. 1 (2021); e002221Revista Brasileira de Medicina Veterinária; v. 43 n. 1 (2021); e0022212527-21790100-2430reponame:Brazilian Journal of Veterinary Medicineinstname:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)instacron:SBMVenghttps://rbmv.org/BJVM/article/view/1198/1100https://rbmv.org/BJVM/article/view/1198/1213Copyright (c) 2021 Mariana Costa Fausto, Luís Henrique Gouvêa Saraiva, Guilherme Costa Fausto, Lorendane Millena de Carvalho, Fabrício Luciani Valente, Marisa Caixeta Valadão, Artur Kanadani Campos, Jackson Victor de Araújohttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessFausto, Mariana CostaSaraiva, Luís Henrique GouvêaFausto, Guilherme Costade Carvalho, Lorendane MillenaValente, Fabrício LucianiValadão, Marisa CaixetaCampos, Artur Kanadanide Araújo, Jackson Victor2022-06-26T22:16:26Zoai:ojs.rbmv.org:article/1198Revistahttps://rbmv.org/BJVMONGhttps://rbmv.org/BJVM/oaicontato.rbmv@gmail.com2527-21790100-2430opendoar:2022-06-26T22:16:26Brazilian Journal of Veterinary Medicine - Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)false |
dc.title.none.fl_str_mv |
Flotation solution influence on the quantification of Ascaris suum eggs in pig feces using McMaster technique Influência da solução de flutuação na quantificação de ovos de Ascaris suum em fezes de suínos utilizando a técnica de McMaster |
title |
Flotation solution influence on the quantification of Ascaris suum eggs in pig feces using McMaster technique |
spellingShingle |
Flotation solution influence on the quantification of Ascaris suum eggs in pig feces using McMaster technique Fausto, Mariana Costa parasitological techniques, sodium nitrate, Tween 80. |
title_short |
Flotation solution influence on the quantification of Ascaris suum eggs in pig feces using McMaster technique |
title_full |
Flotation solution influence on the quantification of Ascaris suum eggs in pig feces using McMaster technique |
title_fullStr |
Flotation solution influence on the quantification of Ascaris suum eggs in pig feces using McMaster technique |
title_full_unstemmed |
Flotation solution influence on the quantification of Ascaris suum eggs in pig feces using McMaster technique |
title_sort |
Flotation solution influence on the quantification of Ascaris suum eggs in pig feces using McMaster technique |
author |
Fausto, Mariana Costa |
author_facet |
Fausto, Mariana Costa Saraiva, Luís Henrique Gouvêa Fausto, Guilherme Costa de Carvalho, Lorendane Millena Valente, Fabrício Luciani Valadão, Marisa Caixeta Campos, Artur Kanadani de Araújo, Jackson Victor |
author_role |
author |
author2 |
Saraiva, Luís Henrique Gouvêa Fausto, Guilherme Costa de Carvalho, Lorendane Millena Valente, Fabrício Luciani Valadão, Marisa Caixeta Campos, Artur Kanadani de Araújo, Jackson Victor |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Fausto, Mariana Costa Saraiva, Luís Henrique Gouvêa Fausto, Guilherme Costa de Carvalho, Lorendane Millena Valente, Fabrício Luciani Valadão, Marisa Caixeta Campos, Artur Kanadani de Araújo, Jackson Victor |
dc.subject.por.fl_str_mv |
parasitological techniques, sodium nitrate, Tween 80. |
topic |
parasitological techniques, sodium nitrate, Tween 80. |
description |
Parasitic diseases are responsible for economic losses in the pig production industry. It is necessary to monitor the parasite load through stool ova and parasite analysis to avoid losses to the producer. Among analytical tests, the McMaster technique is the most widely used method; however, several variations of this method have been described, and, therefore, there is a need for standardization. The aim of this study was to evaluate the use of different saturated solutions prepared from four different salts (NaCl, MgSO4, NaNO3, ZnSO4), a sugar solution, and Polysorbate Tween 80, to perform the McMaster technique. Pig feces containing Ascaris suum eggs were homogenized in Griffin beakers and divided into 2g aliquots for the exams with different solutions. All solutions evaluated were able to float A. suum eggs. However, eggs per gram of feces (EPG) values using the sodium nitrate solution were higher (p < 0.05) than for the other solutions. Magnesium sulfate and sodium chloride solutions provided intermediate values for EPG. Lower values were obtained with zinc sulfate and sugar solutions. Polysorbate Tween 80 was added to the sodium nitrate solution at a concentration of 0.2%, and this proved to be more efficient (p < 0.05) in flotation of the eggs. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-10-07 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion peer reviewed Avaliado pelos pares |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rbmv.org/BJVM/article/view/1198 10.29374/2527-2179.bjvm002221 |
url |
https://rbmv.org/BJVM/article/view/1198 |
identifier_str_mv |
10.29374/2527-2179.bjvm002221 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rbmv.org/BJVM/article/view/1198/1100 https://rbmv.org/BJVM/article/view/1198/1213 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf text/xml |
dc.publisher.none.fl_str_mv |
Sociedade de Medicina Veterinária do Estado do Rio de Janeiro. |
publisher.none.fl_str_mv |
Sociedade de Medicina Veterinária do Estado do Rio de Janeiro. |
dc.source.none.fl_str_mv |
Brazilian Journal of Veterinary Medicine; Vol. 43 No. 1 (2021); e002221 Revista Brasileira de Medicina Veterinária; v. 43 n. 1 (2021); e002221 2527-2179 0100-2430 reponame:Brazilian Journal of Veterinary Medicine instname:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ) instacron:SBMV |
instname_str |
Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ) |
instacron_str |
SBMV |
institution |
SBMV |
reponame_str |
Brazilian Journal of Veterinary Medicine |
collection |
Brazilian Journal of Veterinary Medicine |
repository.name.fl_str_mv |
Brazilian Journal of Veterinary Medicine - Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ) |
repository.mail.fl_str_mv |
contato.rbmv@gmail.com |
_version_ |
1798313111634051072 |