Urinary cytokine profiles according to the site of blockade of the renin-angiotensin system in nephrectomized rats
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Jornal Brasileiro de Nefrologia |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-28002017000200108 |
Resumo: | Abstract Introduction: It is still unknown how the pharmacological inhibition of the Renin Angiotensin System (RAS) impacts the levels of inflammation and fibrosis biomarkers. Objective: This study sought to evaluate the effect of enalapril, candesartan and aliskiren on urinary levels of cytokines in a model of chronic kidney disease (CKD). Methods: Male Wistar rats were submitted to surgical removal of ¾ of renal parenchyma to induce CKD (¾ nephrectomy), or subjected to sham surgery (control). Animals were then randomized into five groups: Sham surgery receiving vehicle; ¾ Nephrectomy receiving vehicle; ¾ Nephrectomy receiving enalapril (10 mg/kg); ¾ Nephrectomy receiving candesartan (10 mg/kg) and ¾ Nephrectomy receiving aliskiren (10 mg/kg). Urine output, water intake, mean arterial pressure (MAP) and urinary concentrations of creatinine, urea, albuminuria, Na+, K+, interleukin (IL) -1β, IL-6, IL-10 and transforming growth factor beta (TGF-β) were measured. Results: Nephrectomy significantly impaired renal function, increased MAP and altered the levels of all evaluated cytokines in urine. Enalapril, candesartan and aliskiren improved renal function and decreased MAP and IL-6 when compared to vehicle-treated nephrectomized group. Candesartan and aliskiren decreased IL-1β, while only candesartan reduced TGF-β and only aliskiren increased IL-10. Conclusion: Enalapril, candesartan and aliskiren presented similar effects on improving renal function and reducing MAP and urinary levels of IL-6 in rats with CKD. On the other hand, cytokine profile differed according to the treatment, suggesting that differential mechanisms were triggered in response to the site of RAS blockade. |
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Urinary cytokine profiles according to the site of blockade of the renin-angiotensin system in nephrectomized ratsangiotensinsangiotensin-converting enzyme inhibitorsangiotensin receptor antagonistskidney failure, chronicnephrectomyAbstract Introduction: It is still unknown how the pharmacological inhibition of the Renin Angiotensin System (RAS) impacts the levels of inflammation and fibrosis biomarkers. Objective: This study sought to evaluate the effect of enalapril, candesartan and aliskiren on urinary levels of cytokines in a model of chronic kidney disease (CKD). Methods: Male Wistar rats were submitted to surgical removal of ¾ of renal parenchyma to induce CKD (¾ nephrectomy), or subjected to sham surgery (control). Animals were then randomized into five groups: Sham surgery receiving vehicle; ¾ Nephrectomy receiving vehicle; ¾ Nephrectomy receiving enalapril (10 mg/kg); ¾ Nephrectomy receiving candesartan (10 mg/kg) and ¾ Nephrectomy receiving aliskiren (10 mg/kg). Urine output, water intake, mean arterial pressure (MAP) and urinary concentrations of creatinine, urea, albuminuria, Na+, K+, interleukin (IL) -1β, IL-6, IL-10 and transforming growth factor beta (TGF-β) were measured. Results: Nephrectomy significantly impaired renal function, increased MAP and altered the levels of all evaluated cytokines in urine. Enalapril, candesartan and aliskiren improved renal function and decreased MAP and IL-6 when compared to vehicle-treated nephrectomized group. Candesartan and aliskiren decreased IL-1β, while only candesartan reduced TGF-β and only aliskiren increased IL-10. Conclusion: Enalapril, candesartan and aliskiren presented similar effects on improving renal function and reducing MAP and urinary levels of IL-6 in rats with CKD. On the other hand, cytokine profile differed according to the treatment, suggesting that differential mechanisms were triggered in response to the site of RAS blockade.Sociedade Brasileira de Nefrologia2017-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-28002017000200108Brazilian Journal of Nephrology v.39 n.2 2017reponame:Jornal Brasileiro de Nefrologiainstname:Sociedade Brasileira de Nefrologia (SBN)instacron:SBN10.5935/0101-2800.20170028info:eu-repo/semantics/openAccessBaracho,Nilo César do ValeSilveira,Kátia Daniela daRocha,Natália PessoaCordeiro,Thiago MacedoFeracin,VictorPereira,Regina MariaReis,Marconi Augusto Aguiar dosTeixeira,Mauro MartinsSilva,Ana Cristina Simões eeng2017-08-15T00:00:00Zoai:scielo:S0101-28002017000200108Revistahttp://www.bjn.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||jbn@sbn.org.br2175-82390101-2800opendoar:2017-08-15T00:00Jornal Brasileiro de Nefrologia - Sociedade Brasileira de Nefrologia (SBN)false |
dc.title.none.fl_str_mv |
Urinary cytokine profiles according to the site of blockade of the renin-angiotensin system in nephrectomized rats |
title |
Urinary cytokine profiles according to the site of blockade of the renin-angiotensin system in nephrectomized rats |
spellingShingle |
Urinary cytokine profiles according to the site of blockade of the renin-angiotensin system in nephrectomized rats Baracho,Nilo César do Vale angiotensins angiotensin-converting enzyme inhibitors angiotensin receptor antagonists kidney failure, chronic nephrectomy |
title_short |
Urinary cytokine profiles according to the site of blockade of the renin-angiotensin system in nephrectomized rats |
title_full |
Urinary cytokine profiles according to the site of blockade of the renin-angiotensin system in nephrectomized rats |
title_fullStr |
Urinary cytokine profiles according to the site of blockade of the renin-angiotensin system in nephrectomized rats |
title_full_unstemmed |
Urinary cytokine profiles according to the site of blockade of the renin-angiotensin system in nephrectomized rats |
title_sort |
Urinary cytokine profiles according to the site of blockade of the renin-angiotensin system in nephrectomized rats |
author |
Baracho,Nilo César do Vale |
author_facet |
Baracho,Nilo César do Vale Silveira,Kátia Daniela da Rocha,Natália Pessoa Cordeiro,Thiago Macedo Feracin,Victor Pereira,Regina Maria Reis,Marconi Augusto Aguiar dos Teixeira,Mauro Martins Silva,Ana Cristina Simões e |
author_role |
author |
author2 |
Silveira,Kátia Daniela da Rocha,Natália Pessoa Cordeiro,Thiago Macedo Feracin,Victor Pereira,Regina Maria Reis,Marconi Augusto Aguiar dos Teixeira,Mauro Martins Silva,Ana Cristina Simões e |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Baracho,Nilo César do Vale Silveira,Kátia Daniela da Rocha,Natália Pessoa Cordeiro,Thiago Macedo Feracin,Victor Pereira,Regina Maria Reis,Marconi Augusto Aguiar dos Teixeira,Mauro Martins Silva,Ana Cristina Simões e |
dc.subject.por.fl_str_mv |
angiotensins angiotensin-converting enzyme inhibitors angiotensin receptor antagonists kidney failure, chronic nephrectomy |
topic |
angiotensins angiotensin-converting enzyme inhibitors angiotensin receptor antagonists kidney failure, chronic nephrectomy |
description |
Abstract Introduction: It is still unknown how the pharmacological inhibition of the Renin Angiotensin System (RAS) impacts the levels of inflammation and fibrosis biomarkers. Objective: This study sought to evaluate the effect of enalapril, candesartan and aliskiren on urinary levels of cytokines in a model of chronic kidney disease (CKD). Methods: Male Wistar rats were submitted to surgical removal of ¾ of renal parenchyma to induce CKD (¾ nephrectomy), or subjected to sham surgery (control). Animals were then randomized into five groups: Sham surgery receiving vehicle; ¾ Nephrectomy receiving vehicle; ¾ Nephrectomy receiving enalapril (10 mg/kg); ¾ Nephrectomy receiving candesartan (10 mg/kg) and ¾ Nephrectomy receiving aliskiren (10 mg/kg). Urine output, water intake, mean arterial pressure (MAP) and urinary concentrations of creatinine, urea, albuminuria, Na+, K+, interleukin (IL) -1β, IL-6, IL-10 and transforming growth factor beta (TGF-β) were measured. Results: Nephrectomy significantly impaired renal function, increased MAP and altered the levels of all evaluated cytokines in urine. Enalapril, candesartan and aliskiren improved renal function and decreased MAP and IL-6 when compared to vehicle-treated nephrectomized group. Candesartan and aliskiren decreased IL-1β, while only candesartan reduced TGF-β and only aliskiren increased IL-10. Conclusion: Enalapril, candesartan and aliskiren presented similar effects on improving renal function and reducing MAP and urinary levels of IL-6 in rats with CKD. On the other hand, cytokine profile differed according to the treatment, suggesting that differential mechanisms were triggered in response to the site of RAS blockade. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-28002017000200108 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-28002017000200108 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.5935/0101-2800.20170028 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Nefrologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Nefrologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Nephrology v.39 n.2 2017 reponame:Jornal Brasileiro de Nefrologia instname:Sociedade Brasileira de Nefrologia (SBN) instacron:SBN |
instname_str |
Sociedade Brasileira de Nefrologia (SBN) |
instacron_str |
SBN |
institution |
SBN |
reponame_str |
Jornal Brasileiro de Nefrologia |
collection |
Jornal Brasileiro de Nefrologia |
repository.name.fl_str_mv |
Jornal Brasileiro de Nefrologia - Sociedade Brasileira de Nefrologia (SBN) |
repository.mail.fl_str_mv |
||jbn@sbn.org.br |
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1752122064250077184 |