Effect of hyaluronic acid on chondrocyte apoptosis

Detalhes bibliográficos
Autor(a) principal: Barreto,Ronald Bispo
Data de Publicação: 2015
Outros Autores: Sadigursky,David, Rezende,Marcia Uchoa de, Hernandez,Arnaldo José
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Acta Ortopédica Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-78522015000200090
Resumo: OBJECTIVE: To determine the percentage of apoptotic cells in a contusion model of osteoarthritis (OA) and to assess whether intra-articular injection of high doses of hyaluronic acid (HA) immediately after trauma reduces chondrocyte apoptosis.METHODS: Forty knees from adult rabbits were impacted thrice with a 1 kg block released through a 1 meter tall cylinder (29.4 Joules). Subsequently, 2 mL of HA was injected in one knee and 2 mL saline in the contra-lateral knee. Medication were administered twice a week for 30 days, when animals were sacrificed. Specimens were prepared for optical microscopy exam and terminal deoxynucleotidyl transferase end labeling assay (TUNEL).RESULTS: The apoptosis rate in the contusion model was 68.01% (± 19.73%), a higher rate than previously described. HA significantly reduced the rate of apoptosis to 53.52% (± 18.09) (p <0.001).CONCLUSION: Intra-articular HA administration started immediately after trauma reduces impact-induced chondrocyte apoptosis rates in rabbits. Level of Evidence I, Experimental Study.
id SBOT-1_e86f7ddfe31b220c421ab8039090ce3e
oai_identifier_str oai:scielo:S1413-78522015000200090
network_acronym_str SBOT-1
network_name_str Acta Ortopédica Brasileira (Online)
repository_id_str
spelling Effect of hyaluronic acid on chondrocyte apoptosisChondrocytesHyaluronic acidApoptosisRabbitsKneeOsteoarthritisOBJECTIVE: To determine the percentage of apoptotic cells in a contusion model of osteoarthritis (OA) and to assess whether intra-articular injection of high doses of hyaluronic acid (HA) immediately after trauma reduces chondrocyte apoptosis.METHODS: Forty knees from adult rabbits were impacted thrice with a 1 kg block released through a 1 meter tall cylinder (29.4 Joules). Subsequently, 2 mL of HA was injected in one knee and 2 mL saline in the contra-lateral knee. Medication were administered twice a week for 30 days, when animals were sacrificed. Specimens were prepared for optical microscopy exam and terminal deoxynucleotidyl transferase end labeling assay (TUNEL).RESULTS: The apoptosis rate in the contusion model was 68.01% (± 19.73%), a higher rate than previously described. HA significantly reduced the rate of apoptosis to 53.52% (± 18.09) (p <0.001).CONCLUSION: Intra-articular HA administration started immediately after trauma reduces impact-induced chondrocyte apoptosis rates in rabbits. Level of Evidence I, Experimental Study.ATHA EDITORA2015-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-78522015000200090Acta Ortopédica Brasileira v.23 n.2 2015reponame:Acta Ortopédica Brasileira (Online)instname:Sociedade Brasileira de Ortopedia e Traumatologia (SBOT)instacron:SBOT10.1590/1413-785220152302144341info:eu-repo/semantics/openAccessBarreto,Ronald BispoSadigursky,DavidRezende,Marcia Uchoa deHernandez,Arnaldo Joséeng2015-10-27T00:00:00Zoai:scielo:S1413-78522015000200090Revistahttp://www.actaortopedica.com.br/https://old.scielo.br/oai/scielo-oai.php1atha@uol.com.br||actaortopedicabrasileira@uol.com.br1809-44061413-7852opendoar:2015-10-27T00:00Acta Ortopédica Brasileira (Online) - Sociedade Brasileira de Ortopedia e Traumatologia (SBOT)false
dc.title.none.fl_str_mv Effect of hyaluronic acid on chondrocyte apoptosis
title Effect of hyaluronic acid on chondrocyte apoptosis
spellingShingle Effect of hyaluronic acid on chondrocyte apoptosis
Barreto,Ronald Bispo
Chondrocytes
Hyaluronic acid
Apoptosis
Rabbits
Knee
Osteoarthritis
title_short Effect of hyaluronic acid on chondrocyte apoptosis
title_full Effect of hyaluronic acid on chondrocyte apoptosis
title_fullStr Effect of hyaluronic acid on chondrocyte apoptosis
title_full_unstemmed Effect of hyaluronic acid on chondrocyte apoptosis
title_sort Effect of hyaluronic acid on chondrocyte apoptosis
author Barreto,Ronald Bispo
author_facet Barreto,Ronald Bispo
Sadigursky,David
Rezende,Marcia Uchoa de
Hernandez,Arnaldo José
author_role author
author2 Sadigursky,David
Rezende,Marcia Uchoa de
Hernandez,Arnaldo José
author2_role author
author
author
dc.contributor.author.fl_str_mv Barreto,Ronald Bispo
Sadigursky,David
Rezende,Marcia Uchoa de
Hernandez,Arnaldo José
dc.subject.por.fl_str_mv Chondrocytes
Hyaluronic acid
Apoptosis
Rabbits
Knee
Osteoarthritis
topic Chondrocytes
Hyaluronic acid
Apoptosis
Rabbits
Knee
Osteoarthritis
description OBJECTIVE: To determine the percentage of apoptotic cells in a contusion model of osteoarthritis (OA) and to assess whether intra-articular injection of high doses of hyaluronic acid (HA) immediately after trauma reduces chondrocyte apoptosis.METHODS: Forty knees from adult rabbits were impacted thrice with a 1 kg block released through a 1 meter tall cylinder (29.4 Joules). Subsequently, 2 mL of HA was injected in one knee and 2 mL saline in the contra-lateral knee. Medication were administered twice a week for 30 days, when animals were sacrificed. Specimens were prepared for optical microscopy exam and terminal deoxynucleotidyl transferase end labeling assay (TUNEL).RESULTS: The apoptosis rate in the contusion model was 68.01% (± 19.73%), a higher rate than previously described. HA significantly reduced the rate of apoptosis to 53.52% (± 18.09) (p <0.001).CONCLUSION: Intra-articular HA administration started immediately after trauma reduces impact-induced chondrocyte apoptosis rates in rabbits. Level of Evidence I, Experimental Study.
publishDate 2015
dc.date.none.fl_str_mv 2015-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-78522015000200090
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-78522015000200090
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1413-785220152302144341
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv ATHA EDITORA
publisher.none.fl_str_mv ATHA EDITORA
dc.source.none.fl_str_mv Acta Ortopédica Brasileira v.23 n.2 2015
reponame:Acta Ortopédica Brasileira (Online)
instname:Sociedade Brasileira de Ortopedia e Traumatologia (SBOT)
instacron:SBOT
instname_str Sociedade Brasileira de Ortopedia e Traumatologia (SBOT)
instacron_str SBOT
institution SBOT
reponame_str Acta Ortopédica Brasileira (Online)
collection Acta Ortopédica Brasileira (Online)
repository.name.fl_str_mv Acta Ortopédica Brasileira (Online) - Sociedade Brasileira de Ortopedia e Traumatologia (SBOT)
repository.mail.fl_str_mv 1atha@uol.com.br||actaortopedicabrasileira@uol.com.br
_version_ 1752122275133390848

Registros relacionados