Protocol for Harvest, Transport and Storage of Human Osteochondral Tissue
Autor(a) principal: | |
---|---|
Data de Publicação: | 2020 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Brasileira de Ortopedia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-36162020000200163 |
Resumo: | Abstract Objective To elaborate a protocol for the harvest, transport, and preservation of human osteochondral tissue for use in tissue banks (TBs). Methods Osteochondral fragments measuring 2 cm3 of 5 corpse donors aged between 15 and 45 years old were analyzed. The samples were stored in cell preservation medium containing: human albumin, Iscove's and vancomycin preserved at 4ºC. The concentration of proteoglycans in the extracellular medium was quantified by the use of Safranin-O, while tissue structural analysis was assessed by histological study with hematoxylin-eosin stained slides. The images obtained were analyzed according to the histological scores of Mankin and the score proposed by the OsteoArthritis Research Society International. The samples were analyzed with 0, 15, 30 and 45 days of preservation. Results The osteochondral fragments studied showed a progressive decrease in proteoglycan concentration with increased preservation time. After 30 days of preservation, structural changes were identified with discontinuity of the cartilage surface layer. According to the results obtained by the Mankin score, there was a statistically significant difference between 15 and 30 days of tissue preservation. Conclusion The protocol described defined knee transport immersed in Lactated Ringer at a controlled temperature of 10º C until its arrival at the TB. After processing, the preservation solution was composed of Iscove's serum-free cell culture medium supplemented with 10% human albumin and 100 µg/ml vancomycin. The tissue was preserved at a temperature of 4ºC until the moment of transplantation characterizing the fresh preservation. |
id |
SBOT-2_12a7c984caa49bf53a0f538ffcce2ee0 |
---|---|
oai_identifier_str |
oai:scielo:S0102-36162020000200163 |
network_acronym_str |
SBOT-2 |
network_name_str |
Revista Brasileira de Ortopedia (Online) |
repository_id_str |
|
spelling |
Protocol for Harvest, Transport and Storage of Human Osteochondral Tissuecartilage, articularallograftsbone transplantationtissue and organ harvestingosteochondral lesionAbstract Objective To elaborate a protocol for the harvest, transport, and preservation of human osteochondral tissue for use in tissue banks (TBs). Methods Osteochondral fragments measuring 2 cm3 of 5 corpse donors aged between 15 and 45 years old were analyzed. The samples were stored in cell preservation medium containing: human albumin, Iscove's and vancomycin preserved at 4ºC. The concentration of proteoglycans in the extracellular medium was quantified by the use of Safranin-O, while tissue structural analysis was assessed by histological study with hematoxylin-eosin stained slides. The images obtained were analyzed according to the histological scores of Mankin and the score proposed by the OsteoArthritis Research Society International. The samples were analyzed with 0, 15, 30 and 45 days of preservation. Results The osteochondral fragments studied showed a progressive decrease in proteoglycan concentration with increased preservation time. After 30 days of preservation, structural changes were identified with discontinuity of the cartilage surface layer. According to the results obtained by the Mankin score, there was a statistically significant difference between 15 and 30 days of tissue preservation. Conclusion The protocol described defined knee transport immersed in Lactated Ringer at a controlled temperature of 10º C until its arrival at the TB. After processing, the preservation solution was composed of Iscove's serum-free cell culture medium supplemented with 10% human albumin and 100 µg/ml vancomycin. The tissue was preserved at a temperature of 4ºC until the moment of transplantation characterizing the fresh preservation.Sociedade Brasileira de Ortopedia e Traumatologia2020-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-36162020000200163Revista Brasileira de Ortopedia v.55 n.2 2020reponame:Revista Brasileira de Ortopedia (Online)instname:Sociedade Brasileira de Ortopedia e Traumatologia (SBOT)instacron:SBOT10.1055/s-0039-3400522info:eu-repo/semantics/openAccessVivacqua,Thiago AlbertoPrinz,Rafael DantasCavanellas,NaassonBarretto,João MaurícioSousa,Eduardo Branco deAguiar,Diego Pinheiroeng2020-05-12T00:00:00Zoai:scielo:S0102-36162020000200163Revistahttp://www.rbo.org.br/https://old.scielo.br/oai/scielo-oai.php||rbo@sbot.org.br1982-43780102-3616opendoar:2020-05-12T00:00Revista Brasileira de Ortopedia (Online) - Sociedade Brasileira de Ortopedia e Traumatologia (SBOT)false |
dc.title.none.fl_str_mv |
Protocol for Harvest, Transport and Storage of Human Osteochondral Tissue |
title |
Protocol for Harvest, Transport and Storage of Human Osteochondral Tissue |
spellingShingle |
Protocol for Harvest, Transport and Storage of Human Osteochondral Tissue Vivacqua,Thiago Alberto cartilage, articular allografts bone transplantation tissue and organ harvesting osteochondral lesion |
title_short |
Protocol for Harvest, Transport and Storage of Human Osteochondral Tissue |
title_full |
Protocol for Harvest, Transport and Storage of Human Osteochondral Tissue |
title_fullStr |
Protocol for Harvest, Transport and Storage of Human Osteochondral Tissue |
title_full_unstemmed |
Protocol for Harvest, Transport and Storage of Human Osteochondral Tissue |
title_sort |
Protocol for Harvest, Transport and Storage of Human Osteochondral Tissue |
author |
Vivacqua,Thiago Alberto |
author_facet |
Vivacqua,Thiago Alberto Prinz,Rafael Dantas Cavanellas,Naasson Barretto,João Maurício Sousa,Eduardo Branco de Aguiar,Diego Pinheiro |
author_role |
author |
author2 |
Prinz,Rafael Dantas Cavanellas,Naasson Barretto,João Maurício Sousa,Eduardo Branco de Aguiar,Diego Pinheiro |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Vivacqua,Thiago Alberto Prinz,Rafael Dantas Cavanellas,Naasson Barretto,João Maurício Sousa,Eduardo Branco de Aguiar,Diego Pinheiro |
dc.subject.por.fl_str_mv |
cartilage, articular allografts bone transplantation tissue and organ harvesting osteochondral lesion |
topic |
cartilage, articular allografts bone transplantation tissue and organ harvesting osteochondral lesion |
description |
Abstract Objective To elaborate a protocol for the harvest, transport, and preservation of human osteochondral tissue for use in tissue banks (TBs). Methods Osteochondral fragments measuring 2 cm3 of 5 corpse donors aged between 15 and 45 years old were analyzed. The samples were stored in cell preservation medium containing: human albumin, Iscove's and vancomycin preserved at 4ºC. The concentration of proteoglycans in the extracellular medium was quantified by the use of Safranin-O, while tissue structural analysis was assessed by histological study with hematoxylin-eosin stained slides. The images obtained were analyzed according to the histological scores of Mankin and the score proposed by the OsteoArthritis Research Society International. The samples were analyzed with 0, 15, 30 and 45 days of preservation. Results The osteochondral fragments studied showed a progressive decrease in proteoglycan concentration with increased preservation time. After 30 days of preservation, structural changes were identified with discontinuity of the cartilage surface layer. According to the results obtained by the Mankin score, there was a statistically significant difference between 15 and 30 days of tissue preservation. Conclusion The protocol described defined knee transport immersed in Lactated Ringer at a controlled temperature of 10º C until its arrival at the TB. After processing, the preservation solution was composed of Iscove's serum-free cell culture medium supplemented with 10% human albumin and 100 µg/ml vancomycin. The tissue was preserved at a temperature of 4ºC until the moment of transplantation characterizing the fresh preservation. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-36162020000200163 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-36162020000200163 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1055/s-0039-3400522 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Ortopedia e Traumatologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Ortopedia e Traumatologia |
dc.source.none.fl_str_mv |
Revista Brasileira de Ortopedia v.55 n.2 2020 reponame:Revista Brasileira de Ortopedia (Online) instname:Sociedade Brasileira de Ortopedia e Traumatologia (SBOT) instacron:SBOT |
instname_str |
Sociedade Brasileira de Ortopedia e Traumatologia (SBOT) |
instacron_str |
SBOT |
institution |
SBOT |
reponame_str |
Revista Brasileira de Ortopedia (Online) |
collection |
Revista Brasileira de Ortopedia (Online) |
repository.name.fl_str_mv |
Revista Brasileira de Ortopedia (Online) - Sociedade Brasileira de Ortopedia e Traumatologia (SBOT) |
repository.mail.fl_str_mv |
||rbo@sbot.org.br |
_version_ |
1752122362382254080 |