Diagnostic performance between histopathological and molecular methods in the detection of Helicobacter pylori
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442021000100418 |
Resumo: | ABSTRACT Introduction: Helicobacter pylori (H. pylori) is a Gram negative bacterium considered to be the etiologic agent of various gastric diseases. The prevalence of bacterial infection varies according to age, geographic location, ethnicity and socioeconomic status. The chronic infection caused by microorganism can favor the development of severe pathologies such as gastric adenocarcinoma. In this sense, early diagnosis is essential for a better prognosis and therapeutic success. Several diagnostic methods performed using invasive and non-invasive techniques, with different sensitivity and specificity, have been used in the detection of H. pylori. Objective: To compare the performance of the molecular and histopathological technique used in the diagnosis of H. pylori infection. Methods: 76 gastric tissue samples were collected from dyspeptic patients who underwent molecular and histopathological diagnosis. Molecular detection was performed using the ribosomal gene (16S rRNA) using the polymerase chain reaction (PCR) technique. Results: The PCR-based molecular diagnostic method detected the bacterium in 63.1% of the samples, while the histopathological test identified the microorganism in only 38.1% of gastric biopsies. The data demonstrated that the PCR technique was about 1.6 times more sensitive than the histopathological technique. Conclusion: The PCR technique was the most efficient diagnostic method for detecting H. pylori and can be implemented in the laboratory routine as a complementary test for the early detection of H. pylori. |
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Diagnostic performance between histopathological and molecular methods in the detection of Helicobacter pyloribacteriamolecular biologyhistologypathologyABSTRACT Introduction: Helicobacter pylori (H. pylori) is a Gram negative bacterium considered to be the etiologic agent of various gastric diseases. The prevalence of bacterial infection varies according to age, geographic location, ethnicity and socioeconomic status. The chronic infection caused by microorganism can favor the development of severe pathologies such as gastric adenocarcinoma. In this sense, early diagnosis is essential for a better prognosis and therapeutic success. Several diagnostic methods performed using invasive and non-invasive techniques, with different sensitivity and specificity, have been used in the detection of H. pylori. Objective: To compare the performance of the molecular and histopathological technique used in the diagnosis of H. pylori infection. Methods: 76 gastric tissue samples were collected from dyspeptic patients who underwent molecular and histopathological diagnosis. Molecular detection was performed using the ribosomal gene (16S rRNA) using the polymerase chain reaction (PCR) technique. Results: The PCR-based molecular diagnostic method detected the bacterium in 63.1% of the samples, while the histopathological test identified the microorganism in only 38.1% of gastric biopsies. The data demonstrated that the PCR technique was about 1.6 times more sensitive than the histopathological technique. Conclusion: The PCR technique was the most efficient diagnostic method for detecting H. pylori and can be implemented in the laboratory routine as a complementary test for the early detection of H. pylori.Sociedade Brasileira de Patologia Clínica2021-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442021000100418Jornal Brasileiro de Patologia e Medicina Laboratorial v.57 2021reponame:Jornal Brasileiro de Patologia e Medicina Laboratorial (Online)instname:Sociedade Brasileira de Patologia (SBP)instacron:SBP10.5935/1676-2444.20210037info:eu-repo/semantics/openAccessSilva,Lucas Luiz L.Calassa,Igor M. C.Oliveira,Ana Karoline S.Ramos,Amanda F. P. L.Pontes,Jaqueline C.Cardoso,Daniela M. M.Rasmussem,Lucas T.Carneiro,Lilian CarlaBarbosa,Monica S.eng2021-11-25T00:00:00Zoai:scielo:S1676-24442021000100418Revistahttp://www.scielo.br/jbpmlhttps://old.scielo.br/oai/scielo-oai.php||jbpml@sbpc.org.br1678-47741676-2444opendoar:2021-11-25T00:00Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) - Sociedade Brasileira de Patologia (SBP)false |
dc.title.none.fl_str_mv |
Diagnostic performance between histopathological and molecular methods in the detection of Helicobacter pylori |
title |
Diagnostic performance between histopathological and molecular methods in the detection of Helicobacter pylori |
spellingShingle |
Diagnostic performance between histopathological and molecular methods in the detection of Helicobacter pylori Silva,Lucas Luiz L. bacteria molecular biology histology pathology |
title_short |
Diagnostic performance between histopathological and molecular methods in the detection of Helicobacter pylori |
title_full |
Diagnostic performance between histopathological and molecular methods in the detection of Helicobacter pylori |
title_fullStr |
Diagnostic performance between histopathological and molecular methods in the detection of Helicobacter pylori |
title_full_unstemmed |
Diagnostic performance between histopathological and molecular methods in the detection of Helicobacter pylori |
title_sort |
Diagnostic performance between histopathological and molecular methods in the detection of Helicobacter pylori |
author |
Silva,Lucas Luiz L. |
author_facet |
Silva,Lucas Luiz L. Calassa,Igor M. C. Oliveira,Ana Karoline S. Ramos,Amanda F. P. L. Pontes,Jaqueline C. Cardoso,Daniela M. M. Rasmussem,Lucas T. Carneiro,Lilian Carla Barbosa,Monica S. |
author_role |
author |
author2 |
Calassa,Igor M. C. Oliveira,Ana Karoline S. Ramos,Amanda F. P. L. Pontes,Jaqueline C. Cardoso,Daniela M. M. Rasmussem,Lucas T. Carneiro,Lilian Carla Barbosa,Monica S. |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Silva,Lucas Luiz L. Calassa,Igor M. C. Oliveira,Ana Karoline S. Ramos,Amanda F. P. L. Pontes,Jaqueline C. Cardoso,Daniela M. M. Rasmussem,Lucas T. Carneiro,Lilian Carla Barbosa,Monica S. |
dc.subject.por.fl_str_mv |
bacteria molecular biology histology pathology |
topic |
bacteria molecular biology histology pathology |
description |
ABSTRACT Introduction: Helicobacter pylori (H. pylori) is a Gram negative bacterium considered to be the etiologic agent of various gastric diseases. The prevalence of bacterial infection varies according to age, geographic location, ethnicity and socioeconomic status. The chronic infection caused by microorganism can favor the development of severe pathologies such as gastric adenocarcinoma. In this sense, early diagnosis is essential for a better prognosis and therapeutic success. Several diagnostic methods performed using invasive and non-invasive techniques, with different sensitivity and specificity, have been used in the detection of H. pylori. Objective: To compare the performance of the molecular and histopathological technique used in the diagnosis of H. pylori infection. Methods: 76 gastric tissue samples were collected from dyspeptic patients who underwent molecular and histopathological diagnosis. Molecular detection was performed using the ribosomal gene (16S rRNA) using the polymerase chain reaction (PCR) technique. Results: The PCR-based molecular diagnostic method detected the bacterium in 63.1% of the samples, while the histopathological test identified the microorganism in only 38.1% of gastric biopsies. The data demonstrated that the PCR technique was about 1.6 times more sensitive than the histopathological technique. Conclusion: The PCR technique was the most efficient diagnostic method for detecting H. pylori and can be implemented in the laboratory routine as a complementary test for the early detection of H. pylori. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442021000100418 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442021000100418 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.5935/1676-2444.20210037 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Patologia Clínica |
publisher.none.fl_str_mv |
Sociedade Brasileira de Patologia Clínica |
dc.source.none.fl_str_mv |
Jornal Brasileiro de Patologia e Medicina Laboratorial v.57 2021 reponame:Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) instname:Sociedade Brasileira de Patologia (SBP) instacron:SBP |
instname_str |
Sociedade Brasileira de Patologia (SBP) |
instacron_str |
SBP |
institution |
SBP |
reponame_str |
Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) |
collection |
Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) |
repository.name.fl_str_mv |
Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) - Sociedade Brasileira de Patologia (SBP) |
repository.mail.fl_str_mv |
||jbpml@sbpc.org.br |
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1752122297866518528 |