Isolation of the wild-type rubella virus in rabbit kidney cell line RC-IAL
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442004000200004 |
Resumo: | The present study describes the isolation and growth the wild-type rubella virus in RC-IAL. The susceptibility of a rabbit kidney (RC-IAL) to the RV RA 27/3 strain was previously reported by us(4). Samples of 20 patients with suspected cases of rubella infection were tested by serology, virus isolation and nested PCR. The sera were tested by Elisa for the detection of rubella virus antibodies and nested PCR assay was performed to detect RNA virus. The samples of urine, blood, fragments of placenta and amniotic fluid were inoculated in the RC-IAL (rabbit kidney, Institute Adolfo Lutz) and Sirc (rabbit cornea) cell lines. Rubella virus was isolated in samples from 14 of the patients. The CPE (cytopathic effect) was visualized by phase contrast and RV RNA was amplified by nested PCR.The results obtained showed that the cellular lineage RC-IAL is an excellent substratum for isolation of the rubella virus. These findings are important since this is one the few cell lines described in literature that presents a cytopathic effect, so that it can be used to isolate the virus of the clinical specimens. |
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Isolation of the wild-type rubella virus in rabbit kidney cell line RC-IALRubella virusIsolationCell lineCytopathic effectThe present study describes the isolation and growth the wild-type rubella virus in RC-IAL. The susceptibility of a rabbit kidney (RC-IAL) to the RV RA 27/3 strain was previously reported by us(4). Samples of 20 patients with suspected cases of rubella infection were tested by serology, virus isolation and nested PCR. The sera were tested by Elisa for the detection of rubella virus antibodies and nested PCR assay was performed to detect RNA virus. The samples of urine, blood, fragments of placenta and amniotic fluid were inoculated in the RC-IAL (rabbit kidney, Institute Adolfo Lutz) and Sirc (rabbit cornea) cell lines. Rubella virus was isolated in samples from 14 of the patients. The CPE (cytopathic effect) was visualized by phase contrast and RV RNA was amplified by nested PCR.The results obtained showed that the cellular lineage RC-IAL is an excellent substratum for isolation of the rubella virus. These findings are important since this is one the few cell lines described in literature that presents a cytopathic effect, so that it can be used to isolate the virus of the clinical specimens.Sociedade Brasileira de Patologia Clínica2004-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442004000200004Jornal Brasileiro de Patologia e Medicina Laboratorial v.40 n.2 2004reponame:Jornal Brasileiro de Patologia e Medicina Laboratorial (Online)instname:Sociedade Brasileira de Patologia (SBP)instacron:SBP10.1590/S1676-24442004000200004info:eu-repo/semantics/openAccessFigueiredo,Cristina A.Oliveira,Maria I.Afonso,Ana M. S.Theobaldo,MárciaCruz,Aurea S.Curti,Suely P.eng2004-06-21T00:00:00Zoai:scielo:S1676-24442004000200004Revistahttp://www.scielo.br/jbpmlhttps://old.scielo.br/oai/scielo-oai.php||jbpml@sbpc.org.br1678-47741676-2444opendoar:2004-06-21T00:00Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) - Sociedade Brasileira de Patologia (SBP)false |
dc.title.none.fl_str_mv |
Isolation of the wild-type rubella virus in rabbit kidney cell line RC-IAL |
title |
Isolation of the wild-type rubella virus in rabbit kidney cell line RC-IAL |
spellingShingle |
Isolation of the wild-type rubella virus in rabbit kidney cell line RC-IAL Figueiredo,Cristina A. Rubella virus Isolation Cell line Cytopathic effect |
title_short |
Isolation of the wild-type rubella virus in rabbit kidney cell line RC-IAL |
title_full |
Isolation of the wild-type rubella virus in rabbit kidney cell line RC-IAL |
title_fullStr |
Isolation of the wild-type rubella virus in rabbit kidney cell line RC-IAL |
title_full_unstemmed |
Isolation of the wild-type rubella virus in rabbit kidney cell line RC-IAL |
title_sort |
Isolation of the wild-type rubella virus in rabbit kidney cell line RC-IAL |
author |
Figueiredo,Cristina A. |
author_facet |
Figueiredo,Cristina A. Oliveira,Maria I. Afonso,Ana M. S. Theobaldo,Márcia Cruz,Aurea S. Curti,Suely P. |
author_role |
author |
author2 |
Oliveira,Maria I. Afonso,Ana M. S. Theobaldo,Márcia Cruz,Aurea S. Curti,Suely P. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Figueiredo,Cristina A. Oliveira,Maria I. Afonso,Ana M. S. Theobaldo,Márcia Cruz,Aurea S. Curti,Suely P. |
dc.subject.por.fl_str_mv |
Rubella virus Isolation Cell line Cytopathic effect |
topic |
Rubella virus Isolation Cell line Cytopathic effect |
description |
The present study describes the isolation and growth the wild-type rubella virus in RC-IAL. The susceptibility of a rabbit kidney (RC-IAL) to the RV RA 27/3 strain was previously reported by us(4). Samples of 20 patients with suspected cases of rubella infection were tested by serology, virus isolation and nested PCR. The sera were tested by Elisa for the detection of rubella virus antibodies and nested PCR assay was performed to detect RNA virus. The samples of urine, blood, fragments of placenta and amniotic fluid were inoculated in the RC-IAL (rabbit kidney, Institute Adolfo Lutz) and Sirc (rabbit cornea) cell lines. Rubella virus was isolated in samples from 14 of the patients. The CPE (cytopathic effect) was visualized by phase contrast and RV RNA was amplified by nested PCR.The results obtained showed that the cellular lineage RC-IAL is an excellent substratum for isolation of the rubella virus. These findings are important since this is one the few cell lines described in literature that presents a cytopathic effect, so that it can be used to isolate the virus of the clinical specimens. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442004000200004 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442004000200004 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1676-24442004000200004 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Patologia Clínica |
publisher.none.fl_str_mv |
Sociedade Brasileira de Patologia Clínica |
dc.source.none.fl_str_mv |
Jornal Brasileiro de Patologia e Medicina Laboratorial v.40 n.2 2004 reponame:Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) instname:Sociedade Brasileira de Patologia (SBP) instacron:SBP |
instname_str |
Sociedade Brasileira de Patologia (SBP) |
instacron_str |
SBP |
institution |
SBP |
reponame_str |
Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) |
collection |
Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) |
repository.name.fl_str_mv |
Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) - Sociedade Brasileira de Patologia (SBP) |
repository.mail.fl_str_mv |
||jbpml@sbpc.org.br |
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1752122293561065472 |