Evaluation of cytokines in type 1 diabetes patients with and without retinopathy

• Autor(a) principal: Cardoso,Jéssica F.
• Data de Publicação: 2017
• Outros Autores: Gomes,Karina B., Fernandes,Ana Paula, Domingueti,Caroline P.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Jornal Brasileiro de Patologia e Medicina Laboratorial (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442017000100031
• topic: diabetes mellitus type 1; diabetic retinopathy; cytokines; inflammation

ABSTRACT Introduction: Diabetes mellitus is one of the greatest public health problems in the world; the numerous complications of this disease are the focus of great concern, as they are related to high morbidity and mortality. Among the complications of diabetes, diabetic retinopathy is the most common microvascular complication. Objectives: Evaluate the association of plasma levels of inflammatory cytokines interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), and interferon-gamma (IFN-γ), and anti-inflammatory cytokine interleukin-10 (IL-10) with diabetic retinopathy and the correlation of plasma levels of these cytokines together. Material and method: 125 patients with type 1 diabetes mellitus were included in this study - 55 with retinopathy and 70 without retinopathy. Cytokines IL-6, TNF-α, IFN-γ and IL-10 were determined by flow cytometry. Results and discussion: A significant association of the levels of cytokines IL-6, TNF-α, IFN-γ and IL-10 with retinopathy was not observed, possibly because measurements were performed on plasma samples, but not on vitreous humor. However, a positive correlation of cytokines among themselves (p < 0.001) was observed. Conclusion: Cytokines IL-6, TNF-α, IFN-γ and IL-10 presented a positive correlation among themselves, what suggests that they may act together in the development and progression of vascular complications in diabetes patients.