Immunohistochemical study of the cellular immune response in human Pneumocystis carinii pneumonia

Detalhes bibliográficos
Autor(a) principal: Castro,Leandro Mantovani de
Data de Publicação: 2006
Outros Autores: Pagliari,Carla, Fernandes,Elaine Raniero, Brasil,Roosecelis Araújo, Duarte,Maria Irma Seixas
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Jornal Brasileiro de Patologia e Medicina Laboratorial (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442006000100002
Resumo: OBJECTIVES: It has been experimentally demonstrated that host defense against Pneumocystis carinii depends on complex interactions within host immune response, mainly CD4 lymphocytes and alveolar macrophages. Since this is an important agent related to immunodeficiency, our purpose was to characterize the inflammatory immune response in lung from necropsy of AIDS patients. PROCEDURES: Twenty-five necropsies with diagnosis of Pneumocystis carinii pneumonia were selected for immunohistochemical investigation of CD4 and CD8 lymphocytes, macrophages (CD68+), NK cells (CD57+) and cells expressing TNF-alpha. The immunostained cells were quantified and statistically analyzed. RESULTS: All specimens presented a great number of cysts of Pneumocystis carinii in alveoli, as well as septal enlargement with inflammatory infiltrate constituted predominantly by lymphocytes and macrophages. CD4+ T cells were decreased in number, and CD8+ T cells, NK cells and macrophages predominated. Cells expressing TNF-alpha were frequently observed in septal inflammatory infiltrate. CONCLUSIONS: The immunosupression related to AIDS induces a reduction in the number of CD4+ T cells and influences high-level parasitism. The cell components that characterize the inflammatory infiltrate contribute to the severe lung injury of those patients.
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spelling Immunohistochemical study of the cellular immune response in human Pneumocystis carinii pneumoniaPneumocystis cariniiImmunohistochemistryCellular immune responseOBJECTIVES: It has been experimentally demonstrated that host defense against Pneumocystis carinii depends on complex interactions within host immune response, mainly CD4 lymphocytes and alveolar macrophages. Since this is an important agent related to immunodeficiency, our purpose was to characterize the inflammatory immune response in lung from necropsy of AIDS patients. PROCEDURES: Twenty-five necropsies with diagnosis of Pneumocystis carinii pneumonia were selected for immunohistochemical investigation of CD4 and CD8 lymphocytes, macrophages (CD68+), NK cells (CD57+) and cells expressing TNF-alpha. The immunostained cells were quantified and statistically analyzed. RESULTS: All specimens presented a great number of cysts of Pneumocystis carinii in alveoli, as well as septal enlargement with inflammatory infiltrate constituted predominantly by lymphocytes and macrophages. CD4+ T cells were decreased in number, and CD8+ T cells, NK cells and macrophages predominated. Cells expressing TNF-alpha were frequently observed in septal inflammatory infiltrate. CONCLUSIONS: The immunosupression related to AIDS induces a reduction in the number of CD4+ T cells and influences high-level parasitism. The cell components that characterize the inflammatory infiltrate contribute to the severe lung injury of those patients.Sociedade Brasileira de Patologia Clínica2006-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442006000100002Jornal Brasileiro de Patologia e Medicina Laboratorial v.42 n.1 2006reponame:Jornal Brasileiro de Patologia e Medicina Laboratorial (Online)instname:Sociedade Brasileira de Patologia (SBP)instacron:SBP10.1590/S1676-24442006000100002info:eu-repo/semantics/openAccessCastro,Leandro Mantovani dePagliari,CarlaFernandes,Elaine RanieroBrasil,Roosecelis AraújoDuarte,Maria Irma Seixaseng2006-06-26T00:00:00Zoai:scielo:S1676-24442006000100002Revistahttp://www.scielo.br/jbpmlhttps://old.scielo.br/oai/scielo-oai.php||jbpml@sbpc.org.br1678-47741676-2444opendoar:2006-06-26T00:00Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) - Sociedade Brasileira de Patologia (SBP)false
dc.title.none.fl_str_mv Immunohistochemical study of the cellular immune response in human Pneumocystis carinii pneumonia
title Immunohistochemical study of the cellular immune response in human Pneumocystis carinii pneumonia
spellingShingle Immunohistochemical study of the cellular immune response in human Pneumocystis carinii pneumonia
Castro,Leandro Mantovani de
Pneumocystis carinii
Immunohistochemistry
Cellular immune response
title_short Immunohistochemical study of the cellular immune response in human Pneumocystis carinii pneumonia
title_full Immunohistochemical study of the cellular immune response in human Pneumocystis carinii pneumonia
title_fullStr Immunohistochemical study of the cellular immune response in human Pneumocystis carinii pneumonia
title_full_unstemmed Immunohistochemical study of the cellular immune response in human Pneumocystis carinii pneumonia
title_sort Immunohistochemical study of the cellular immune response in human Pneumocystis carinii pneumonia
author Castro,Leandro Mantovani de
author_facet Castro,Leandro Mantovani de
Pagliari,Carla
Fernandes,Elaine Raniero
Brasil,Roosecelis Araújo
Duarte,Maria Irma Seixas
author_role author
author2 Pagliari,Carla
Fernandes,Elaine Raniero
Brasil,Roosecelis Araújo
Duarte,Maria Irma Seixas
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Castro,Leandro Mantovani de
Pagliari,Carla
Fernandes,Elaine Raniero
Brasil,Roosecelis Araújo
Duarte,Maria Irma Seixas
dc.subject.por.fl_str_mv Pneumocystis carinii
Immunohistochemistry
Cellular immune response
topic Pneumocystis carinii
Immunohistochemistry
Cellular immune response
description OBJECTIVES: It has been experimentally demonstrated that host defense against Pneumocystis carinii depends on complex interactions within host immune response, mainly CD4 lymphocytes and alveolar macrophages. Since this is an important agent related to immunodeficiency, our purpose was to characterize the inflammatory immune response in lung from necropsy of AIDS patients. PROCEDURES: Twenty-five necropsies with diagnosis of Pneumocystis carinii pneumonia were selected for immunohistochemical investigation of CD4 and CD8 lymphocytes, macrophages (CD68+), NK cells (CD57+) and cells expressing TNF-alpha. The immunostained cells were quantified and statistically analyzed. RESULTS: All specimens presented a great number of cysts of Pneumocystis carinii in alveoli, as well as septal enlargement with inflammatory infiltrate constituted predominantly by lymphocytes and macrophages. CD4+ T cells were decreased in number, and CD8+ T cells, NK cells and macrophages predominated. Cells expressing TNF-alpha were frequently observed in septal inflammatory infiltrate. CONCLUSIONS: The immunosupression related to AIDS induces a reduction in the number of CD4+ T cells and influences high-level parasitism. The cell components that characterize the inflammatory infiltrate contribute to the severe lung injury of those patients.
publishDate 2006
dc.date.none.fl_str_mv 2006-02-01
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dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442006000100002
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dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1676-24442006000100002
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dc.publisher.none.fl_str_mv
Sociedade Brasileira de Patologia Clínica
publisher.none.fl_str_mv
Sociedade Brasileira de Patologia Clínica
dc.source.none.fl_str_mv Jornal Brasileiro de Patologia e Medicina Laboratorial v.42 n.1 2006
reponame:Jornal Brasileiro de Patologia e Medicina Laboratorial (Online)
instname:Sociedade Brasileira de Patologia (SBP)
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