Immunohistochemical study of the cellular immune response in human Pneumocystis carinii pneumonia
Autor(a) principal: | |
---|---|
Data de Publicação: | 2006 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442006000100002 |
Resumo: | OBJECTIVES: It has been experimentally demonstrated that host defense against Pneumocystis carinii depends on complex interactions within host immune response, mainly CD4 lymphocytes and alveolar macrophages. Since this is an important agent related to immunodeficiency, our purpose was to characterize the inflammatory immune response in lung from necropsy of AIDS patients. PROCEDURES: Twenty-five necropsies with diagnosis of Pneumocystis carinii pneumonia were selected for immunohistochemical investigation of CD4 and CD8 lymphocytes, macrophages (CD68+), NK cells (CD57+) and cells expressing TNF-alpha. The immunostained cells were quantified and statistically analyzed. RESULTS: All specimens presented a great number of cysts of Pneumocystis carinii in alveoli, as well as septal enlargement with inflammatory infiltrate constituted predominantly by lymphocytes and macrophages. CD4+ T cells were decreased in number, and CD8+ T cells, NK cells and macrophages predominated. Cells expressing TNF-alpha were frequently observed in septal inflammatory infiltrate. CONCLUSIONS: The immunosupression related to AIDS induces a reduction in the number of CD4+ T cells and influences high-level parasitism. The cell components that characterize the inflammatory infiltrate contribute to the severe lung injury of those patients. |
id |
SBP-1_c4d6cca0fb9475858d6e7d88a29459ab |
---|---|
oai_identifier_str |
oai:scielo:S1676-24442006000100002 |
network_acronym_str |
SBP-1 |
network_name_str |
Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) |
repository_id_str |
|
spelling |
Immunohistochemical study of the cellular immune response in human Pneumocystis carinii pneumoniaPneumocystis cariniiImmunohistochemistryCellular immune responseOBJECTIVES: It has been experimentally demonstrated that host defense against Pneumocystis carinii depends on complex interactions within host immune response, mainly CD4 lymphocytes and alveolar macrophages. Since this is an important agent related to immunodeficiency, our purpose was to characterize the inflammatory immune response in lung from necropsy of AIDS patients. PROCEDURES: Twenty-five necropsies with diagnosis of Pneumocystis carinii pneumonia were selected for immunohistochemical investigation of CD4 and CD8 lymphocytes, macrophages (CD68+), NK cells (CD57+) and cells expressing TNF-alpha. The immunostained cells were quantified and statistically analyzed. RESULTS: All specimens presented a great number of cysts of Pneumocystis carinii in alveoli, as well as septal enlargement with inflammatory infiltrate constituted predominantly by lymphocytes and macrophages. CD4+ T cells were decreased in number, and CD8+ T cells, NK cells and macrophages predominated. Cells expressing TNF-alpha were frequently observed in septal inflammatory infiltrate. CONCLUSIONS: The immunosupression related to AIDS induces a reduction in the number of CD4+ T cells and influences high-level parasitism. The cell components that characterize the inflammatory infiltrate contribute to the severe lung injury of those patients.Sociedade Brasileira de Patologia Clínica2006-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442006000100002Jornal Brasileiro de Patologia e Medicina Laboratorial v.42 n.1 2006reponame:Jornal Brasileiro de Patologia e Medicina Laboratorial (Online)instname:Sociedade Brasileira de Patologia (SBP)instacron:SBP10.1590/S1676-24442006000100002info:eu-repo/semantics/openAccessCastro,Leandro Mantovani dePagliari,CarlaFernandes,Elaine RanieroBrasil,Roosecelis AraújoDuarte,Maria Irma Seixaseng2006-06-26T00:00:00Zoai:scielo:S1676-24442006000100002Revistahttp://www.scielo.br/jbpmlhttps://old.scielo.br/oai/scielo-oai.php||jbpml@sbpc.org.br1678-47741676-2444opendoar:2006-06-26T00:00Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) - Sociedade Brasileira de Patologia (SBP)false |
dc.title.none.fl_str_mv |
Immunohistochemical study of the cellular immune response in human Pneumocystis carinii pneumonia |
title |
Immunohistochemical study of the cellular immune response in human Pneumocystis carinii pneumonia |
spellingShingle |
Immunohistochemical study of the cellular immune response in human Pneumocystis carinii pneumonia Castro,Leandro Mantovani de Pneumocystis carinii Immunohistochemistry Cellular immune response |
title_short |
Immunohistochemical study of the cellular immune response in human Pneumocystis carinii pneumonia |
title_full |
Immunohistochemical study of the cellular immune response in human Pneumocystis carinii pneumonia |
title_fullStr |
Immunohistochemical study of the cellular immune response in human Pneumocystis carinii pneumonia |
title_full_unstemmed |
Immunohistochemical study of the cellular immune response in human Pneumocystis carinii pneumonia |
title_sort |
Immunohistochemical study of the cellular immune response in human Pneumocystis carinii pneumonia |
author |
Castro,Leandro Mantovani de |
author_facet |
Castro,Leandro Mantovani de Pagliari,Carla Fernandes,Elaine Raniero Brasil,Roosecelis Araújo Duarte,Maria Irma Seixas |
author_role |
author |
author2 |
Pagliari,Carla Fernandes,Elaine Raniero Brasil,Roosecelis Araújo Duarte,Maria Irma Seixas |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Castro,Leandro Mantovani de Pagliari,Carla Fernandes,Elaine Raniero Brasil,Roosecelis Araújo Duarte,Maria Irma Seixas |
dc.subject.por.fl_str_mv |
Pneumocystis carinii Immunohistochemistry Cellular immune response |
topic |
Pneumocystis carinii Immunohistochemistry Cellular immune response |
description |
OBJECTIVES: It has been experimentally demonstrated that host defense against Pneumocystis carinii depends on complex interactions within host immune response, mainly CD4 lymphocytes and alveolar macrophages. Since this is an important agent related to immunodeficiency, our purpose was to characterize the inflammatory immune response in lung from necropsy of AIDS patients. PROCEDURES: Twenty-five necropsies with diagnosis of Pneumocystis carinii pneumonia were selected for immunohistochemical investigation of CD4 and CD8 lymphocytes, macrophages (CD68+), NK cells (CD57+) and cells expressing TNF-alpha. The immunostained cells were quantified and statistically analyzed. RESULTS: All specimens presented a great number of cysts of Pneumocystis carinii in alveoli, as well as septal enlargement with inflammatory infiltrate constituted predominantly by lymphocytes and macrophages. CD4+ T cells were decreased in number, and CD8+ T cells, NK cells and macrophages predominated. Cells expressing TNF-alpha were frequently observed in septal inflammatory infiltrate. CONCLUSIONS: The immunosupression related to AIDS induces a reduction in the number of CD4+ T cells and influences high-level parasitism. The cell components that characterize the inflammatory infiltrate contribute to the severe lung injury of those patients. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-02-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442006000100002 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442006000100002 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1676-24442006000100002 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Patologia Clínica |
publisher.none.fl_str_mv |
Sociedade Brasileira de Patologia Clínica |
dc.source.none.fl_str_mv |
Jornal Brasileiro de Patologia e Medicina Laboratorial v.42 n.1 2006 reponame:Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) instname:Sociedade Brasileira de Patologia (SBP) instacron:SBP |
instname_str |
Sociedade Brasileira de Patologia (SBP) |
instacron_str |
SBP |
institution |
SBP |
reponame_str |
Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) |
collection |
Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) |
repository.name.fl_str_mv |
Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) - Sociedade Brasileira de Patologia (SBP) |
repository.mail.fl_str_mv |
||jbpml@sbpc.org.br |
_version_ |
1752122293998321664 |