Homocysteine: validation and comparison of two methods using samples from patients with pulmonary hypertension
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442014000600402 |
Resumo: | Introduction and objective:The determination of homocysteine plasma levels has been reported as a risk marker of interest in severe diseases involving endothelial injury and associated with the development or progression of atherosclerotic lesions and thrombus formation. The aims of this study were to validate method for quantification of plasma homocysteine by high performance liquid chromatography (HPLC) with fluorimetric detection, and to compare the results obtained from patients with pulmonary hypertension by HPLC with those obtained by spectrophotometric enzymatic cycling (S-Ec) method.Materials and methods:The validation parameters, such as linearity, matrix effect, precision, accuracy, detection and quantitation limits, and robustness of the method were evaluated aiming to demonstrate that it is suitable for the intended use. The data obtained in the quantification of homocysteine using the validated method (HPLC) and the spectrophotometric enzymatic cycling (S-Ec) method, were compared.Results:The method was precise, accurate, and robust; it also had good recovery and showed no matrix effect. The linearity covered a range of 5.0-85.0 µmol/l and the limits of detection and quantification were 1.0 µmol/l and 3.4 µmol/l, respectively. The results obtained for homocysteine determination by HPLC and S-Ec methods were comparable.Conclusion:The validated HPLC method showed good performance for quantification of plasma homocysteine levels, while S-Ec method provided results for homocysteine comparable with those obtained by the validated method; therefore, this methodology is a potential alternative of automated method for clinical laboratories. |
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Homocysteine: validation and comparison of two methods using samples from patients with pulmonary hypertensionhomocysteinehigh performance liquid chromatographyspectrophotometric enzymatic cycling methodIntroduction and objective:The determination of homocysteine plasma levels has been reported as a risk marker of interest in severe diseases involving endothelial injury and associated with the development or progression of atherosclerotic lesions and thrombus formation. The aims of this study were to validate method for quantification of plasma homocysteine by high performance liquid chromatography (HPLC) with fluorimetric detection, and to compare the results obtained from patients with pulmonary hypertension by HPLC with those obtained by spectrophotometric enzymatic cycling (S-Ec) method.Materials and methods:The validation parameters, such as linearity, matrix effect, precision, accuracy, detection and quantitation limits, and robustness of the method were evaluated aiming to demonstrate that it is suitable for the intended use. The data obtained in the quantification of homocysteine using the validated method (HPLC) and the spectrophotometric enzymatic cycling (S-Ec) method, were compared.Results:The method was precise, accurate, and robust; it also had good recovery and showed no matrix effect. The linearity covered a range of 5.0-85.0 µmol/l and the limits of detection and quantification were 1.0 µmol/l and 3.4 µmol/l, respectively. The results obtained for homocysteine determination by HPLC and S-Ec methods were comparable.Conclusion:The validated HPLC method showed good performance for quantification of plasma homocysteine levels, while S-Ec method provided results for homocysteine comparable with those obtained by the validated method; therefore, this methodology is a potential alternative of automated method for clinical laboratories.Sociedade Brasileira de Patologia Clínica2014-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442014000600402Jornal Brasileiro de Patologia e Medicina Laboratorial v.50 n.6 2014reponame:Jornal Brasileiro de Patologia e Medicina Laboratorial (Online)instname:Sociedade Brasileira de Patologia (SBP)instacron:SBP10.5935/1676-2444.20140048info:eu-repo/semantics/openAccessBarbosa,Tatiana Maria Costa de CamposCarvalho,Maria das GraçasSilveira,Josianne NicácioRios,Júnia GaribKomatsuzaki,FláviaGodói,Lara CarvalhoCosta,Guilherme Hideki Yoshizaneeng2015-10-27T00:00:00Zoai:scielo:S1676-24442014000600402Revistahttp://www.scielo.br/jbpmlhttps://old.scielo.br/oai/scielo-oai.php||jbpml@sbpc.org.br1678-47741676-2444opendoar:2015-10-27T00:00Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) - Sociedade Brasileira de Patologia (SBP)false |
dc.title.none.fl_str_mv |
Homocysteine: validation and comparison of two methods using samples from patients with pulmonary hypertension |
title |
Homocysteine: validation and comparison of two methods using samples from patients with pulmonary hypertension |
spellingShingle |
Homocysteine: validation and comparison of two methods using samples from patients with pulmonary hypertension Barbosa,Tatiana Maria Costa de Campos homocysteine high performance liquid chromatography spectrophotometric enzymatic cycling method |
title_short |
Homocysteine: validation and comparison of two methods using samples from patients with pulmonary hypertension |
title_full |
Homocysteine: validation and comparison of two methods using samples from patients with pulmonary hypertension |
title_fullStr |
Homocysteine: validation and comparison of two methods using samples from patients with pulmonary hypertension |
title_full_unstemmed |
Homocysteine: validation and comparison of two methods using samples from patients with pulmonary hypertension |
title_sort |
Homocysteine: validation and comparison of two methods using samples from patients with pulmonary hypertension |
author |
Barbosa,Tatiana Maria Costa de Campos |
author_facet |
Barbosa,Tatiana Maria Costa de Campos Carvalho,Maria das Graças Silveira,Josianne Nicácio Rios,Júnia Garib Komatsuzaki,Flávia Godói,Lara Carvalho Costa,Guilherme Hideki Yoshizane |
author_role |
author |
author2 |
Carvalho,Maria das Graças Silveira,Josianne Nicácio Rios,Júnia Garib Komatsuzaki,Flávia Godói,Lara Carvalho Costa,Guilherme Hideki Yoshizane |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Barbosa,Tatiana Maria Costa de Campos Carvalho,Maria das Graças Silveira,Josianne Nicácio Rios,Júnia Garib Komatsuzaki,Flávia Godói,Lara Carvalho Costa,Guilherme Hideki Yoshizane |
dc.subject.por.fl_str_mv |
homocysteine high performance liquid chromatography spectrophotometric enzymatic cycling method |
topic |
homocysteine high performance liquid chromatography spectrophotometric enzymatic cycling method |
description |
Introduction and objective:The determination of homocysteine plasma levels has been reported as a risk marker of interest in severe diseases involving endothelial injury and associated with the development or progression of atherosclerotic lesions and thrombus formation. The aims of this study were to validate method for quantification of plasma homocysteine by high performance liquid chromatography (HPLC) with fluorimetric detection, and to compare the results obtained from patients with pulmonary hypertension by HPLC with those obtained by spectrophotometric enzymatic cycling (S-Ec) method.Materials and methods:The validation parameters, such as linearity, matrix effect, precision, accuracy, detection and quantitation limits, and robustness of the method were evaluated aiming to demonstrate that it is suitable for the intended use. The data obtained in the quantification of homocysteine using the validated method (HPLC) and the spectrophotometric enzymatic cycling (S-Ec) method, were compared.Results:The method was precise, accurate, and robust; it also had good recovery and showed no matrix effect. The linearity covered a range of 5.0-85.0 µmol/l and the limits of detection and quantification were 1.0 µmol/l and 3.4 µmol/l, respectively. The results obtained for homocysteine determination by HPLC and S-Ec methods were comparable.Conclusion:The validated HPLC method showed good performance for quantification of plasma homocysteine levels, while S-Ec method provided results for homocysteine comparable with those obtained by the validated method; therefore, this methodology is a potential alternative of automated method for clinical laboratories. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442014000600402 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442014000600402 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.5935/1676-2444.20140048 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Patologia Clínica |
publisher.none.fl_str_mv |
Sociedade Brasileira de Patologia Clínica |
dc.source.none.fl_str_mv |
Jornal Brasileiro de Patologia e Medicina Laboratorial v.50 n.6 2014 reponame:Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) instname:Sociedade Brasileira de Patologia (SBP) instacron:SBP |
instname_str |
Sociedade Brasileira de Patologia (SBP) |
instacron_str |
SBP |
institution |
SBP |
reponame_str |
Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) |
collection |
Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) |
repository.name.fl_str_mv |
Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) - Sociedade Brasileira de Patologia (SBP) |
repository.mail.fl_str_mv |
||jbpml@sbpc.org.br |
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1752122296235982848 |