DNA measurements after radiation-induced tissue structure of thyroid gland of rats
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442005000300006 |
Resumo: | INTRODUCTION: Thyroid gland exposures to radiation induce nuclear chromosomal alteration. Objective: To evaluate the DNA content of thyroid gland submitted to radiation. MATERIALS AND METHODS: We radiated 75 rats while 25 were not radiated to be used as control group. Exposure was conducted by the use of Cobalt-60 radioactive source in the right anterior cervical region in a field of 3-30cm, comprising the second and the sixth tracheal rings with 600-centigray (cGY) doses. The DNA content (ploidy) was obtained with Feulgen-thionin stain and was quantified with CAS 200 quantitative measurement equipment. RESULTS: Diploid pattern was obtained in 88 cases (95.7%), independently of time of exposure: on the other hand, aneuploidy was observed in four cases (4.3%) only in the group sacrificed to the 33 days. Eight cases were excluded due to technical reasons. CONCLUSION: The early aneuploid pattern found in our study certainly corroborated that radiation affects thyroid gland with important consequences in terms of disorders. |
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DNA measurements after radiation-induced tissue structure of thyroid gland of ratsThyroid cancerIrradiationDNA content/ploidyINTRODUCTION: Thyroid gland exposures to radiation induce nuclear chromosomal alteration. Objective: To evaluate the DNA content of thyroid gland submitted to radiation. MATERIALS AND METHODS: We radiated 75 rats while 25 were not radiated to be used as control group. Exposure was conducted by the use of Cobalt-60 radioactive source in the right anterior cervical region in a field of 3-30cm, comprising the second and the sixth tracheal rings with 600-centigray (cGY) doses. The DNA content (ploidy) was obtained with Feulgen-thionin stain and was quantified with CAS 200 quantitative measurement equipment. RESULTS: Diploid pattern was obtained in 88 cases (95.7%), independently of time of exposure: on the other hand, aneuploidy was observed in four cases (4.3%) only in the group sacrificed to the 33 days. Eight cases were excluded due to technical reasons. CONCLUSION: The early aneuploid pattern found in our study certainly corroborated that radiation affects thyroid gland with important consequences in terms of disorders.Sociedade Brasileira de Patologia Clínica2005-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442005000300006Jornal Brasileiro de Patologia e Medicina Laboratorial v.41 n.3 2005reponame:Jornal Brasileiro de Patologia e Medicina Laboratorial (Online)instname:Sociedade Brasileira de Patologia (SBP)instacron:SBP10.1590/S1676-24442005000300006info:eu-repo/semantics/openAccessCamargo,Roberto SouzaShirata,Neuza KasumiSetani,Eliana OgassavaraGarcia,Eduardo AnselmoPompeu,EduardoMartella,EduardoLoreto,Celso diLongatto Filho,Adhemareng2005-11-18T00:00:00Zoai:scielo:S1676-24442005000300006Revistahttp://www.scielo.br/jbpmlhttps://old.scielo.br/oai/scielo-oai.php||jbpml@sbpc.org.br1678-47741676-2444opendoar:2005-11-18T00:00Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) - Sociedade Brasileira de Patologia (SBP)false |
dc.title.none.fl_str_mv |
DNA measurements after radiation-induced tissue structure of thyroid gland of rats |
title |
DNA measurements after radiation-induced tissue structure of thyroid gland of rats |
spellingShingle |
DNA measurements after radiation-induced tissue structure of thyroid gland of rats Camargo,Roberto Souza Thyroid cancer Irradiation DNA content/ploidy |
title_short |
DNA measurements after radiation-induced tissue structure of thyroid gland of rats |
title_full |
DNA measurements after radiation-induced tissue structure of thyroid gland of rats |
title_fullStr |
DNA measurements after radiation-induced tissue structure of thyroid gland of rats |
title_full_unstemmed |
DNA measurements after radiation-induced tissue structure of thyroid gland of rats |
title_sort |
DNA measurements after radiation-induced tissue structure of thyroid gland of rats |
author |
Camargo,Roberto Souza |
author_facet |
Camargo,Roberto Souza Shirata,Neuza Kasumi Setani,Eliana Ogassavara Garcia,Eduardo Anselmo Pompeu,Eduardo Martella,Eduardo Loreto,Celso di Longatto Filho,Adhemar |
author_role |
author |
author2 |
Shirata,Neuza Kasumi Setani,Eliana Ogassavara Garcia,Eduardo Anselmo Pompeu,Eduardo Martella,Eduardo Loreto,Celso di Longatto Filho,Adhemar |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Camargo,Roberto Souza Shirata,Neuza Kasumi Setani,Eliana Ogassavara Garcia,Eduardo Anselmo Pompeu,Eduardo Martella,Eduardo Loreto,Celso di Longatto Filho,Adhemar |
dc.subject.por.fl_str_mv |
Thyroid cancer Irradiation DNA content/ploidy |
topic |
Thyroid cancer Irradiation DNA content/ploidy |
description |
INTRODUCTION: Thyroid gland exposures to radiation induce nuclear chromosomal alteration. Objective: To evaluate the DNA content of thyroid gland submitted to radiation. MATERIALS AND METHODS: We radiated 75 rats while 25 were not radiated to be used as control group. Exposure was conducted by the use of Cobalt-60 radioactive source in the right anterior cervical region in a field of 3-30cm, comprising the second and the sixth tracheal rings with 600-centigray (cGY) doses. The DNA content (ploidy) was obtained with Feulgen-thionin stain and was quantified with CAS 200 quantitative measurement equipment. RESULTS: Diploid pattern was obtained in 88 cases (95.7%), independently of time of exposure: on the other hand, aneuploidy was observed in four cases (4.3%) only in the group sacrificed to the 33 days. Eight cases were excluded due to technical reasons. CONCLUSION: The early aneuploid pattern found in our study certainly corroborated that radiation affects thyroid gland with important consequences in terms of disorders. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442005000300006 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442005000300006 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1676-24442005000300006 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Patologia Clínica |
publisher.none.fl_str_mv |
Sociedade Brasileira de Patologia Clínica |
dc.source.none.fl_str_mv |
Jornal Brasileiro de Patologia e Medicina Laboratorial v.41 n.3 2005 reponame:Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) instname:Sociedade Brasileira de Patologia (SBP) instacron:SBP |
instname_str |
Sociedade Brasileira de Patologia (SBP) |
instacron_str |
SBP |
institution |
SBP |
reponame_str |
Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) |
collection |
Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) |
repository.name.fl_str_mv |
Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) - Sociedade Brasileira de Patologia (SBP) |
repository.mail.fl_str_mv |
||jbpml@sbpc.org.br |
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1752122293685846016 |