Intra- and interobserver agreement of histopathological findings in pediatric patients with eosinophilic esophagitis
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Jornal de Pediatria (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0021-75572022000100026 |
Resumo: | Abstract Objective: To assess intra- and interobserver agreement among non-expert pathologists in identifying features of the eosinophilic esophagitis histologic scoring system (EoEHSS) in pediatric patients. Patients and methods: The authors used 50 slides from patients (aged 1-15 years; 72% male) with EoE. EoEHSS evaluates eosinophilic inflammation and other features including epithelial basal zone hyperplasia, eosinophilic abscesses, eosinophil surface layering, dilated intercellular spaces, surface epithelial alteration, dyskeratotic epithelial cells, and lamina propria fibrosis. Grade and stage of abnormalities are scored using a 4-point scale (0 normal; 3 maximum change). Four pathologists determined EoEHSS findings on two occasions. Intra- and interobserver agreement was assessed using Kappa (κ) statistics and intra-class correlation coefficients. Results: Intra- and interobserver agreement for the identification of eosinophil counts ≥ 15/high power field (HPF) was excellent, however varied when assessing additional features of the EoEHSS. For the more experienced pathologist, agreement for most EoEHSS items and the composite scores was substantial to excellent. For the less experienced pathologists, intraobserver agreement ranged from absent to substantial for individual features and ranged from moderate to substantial for the composite scores. Conclusion: Most items of the EoEHSS had substantial to excellent reliability when assessed by a pathologist experienced in the diagnosis of EoE but presented lower repeatability among less experienced pathologists. These findings suggest that specific training of pathologists is required for the identification of EoEHSS characteristics beyond eosinophil count, as these features are considered useful in the evaluation of response to treatment and correlation with clinical manifestations and endoscopic findings. |
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Jornal de Pediatria (Online) |
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Intra- and interobserver agreement of histopathological findings in pediatric patients with eosinophilic esophagitisEosinophilic esophagitisEndoscopyHistologyObserver variationAbstract Objective: To assess intra- and interobserver agreement among non-expert pathologists in identifying features of the eosinophilic esophagitis histologic scoring system (EoEHSS) in pediatric patients. Patients and methods: The authors used 50 slides from patients (aged 1-15 years; 72% male) with EoE. EoEHSS evaluates eosinophilic inflammation and other features including epithelial basal zone hyperplasia, eosinophilic abscesses, eosinophil surface layering, dilated intercellular spaces, surface epithelial alteration, dyskeratotic epithelial cells, and lamina propria fibrosis. Grade and stage of abnormalities are scored using a 4-point scale (0 normal; 3 maximum change). Four pathologists determined EoEHSS findings on two occasions. Intra- and interobserver agreement was assessed using Kappa (κ) statistics and intra-class correlation coefficients. Results: Intra- and interobserver agreement for the identification of eosinophil counts ≥ 15/high power field (HPF) was excellent, however varied when assessing additional features of the EoEHSS. For the more experienced pathologist, agreement for most EoEHSS items and the composite scores was substantial to excellent. For the less experienced pathologists, intraobserver agreement ranged from absent to substantial for individual features and ranged from moderate to substantial for the composite scores. Conclusion: Most items of the EoEHSS had substantial to excellent reliability when assessed by a pathologist experienced in the diagnosis of EoE but presented lower repeatability among less experienced pathologists. These findings suggest that specific training of pathologists is required for the identification of EoEHSS characteristics beyond eosinophil count, as these features are considered useful in the evaluation of response to treatment and correlation with clinical manifestations and endoscopic findings.Sociedade Brasileira de Pediatria2022-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0021-75572022000100026Jornal de Pediatria v.98 n.1 2022reponame:Jornal de Pediatria (Online)instname:Sociedade Brasileira de Pediatria (SBP)instacron:SBPE10.1016/j.jped.2021.03.004info:eu-repo/semantics/openAccessVieira,Mário CésarGugelmin,Elizabeth SchneiderPercicote,Ana PaulaRibeiro,Melyssa GrignetMiranda,Renata Augusta deVieira,Gabriela GuimãraesRibeiro,Luciana Bandeira MendezTruppel,Sabine KrügerRosário Filho,Nelson Augustoeng2022-02-11T00:00:00Zoai:scielo:S0021-75572022000100026Revistahttp://www.jped.com.br/https://old.scielo.br/oai/scielo-oai.php||jped@jped.com.br1678-47820021-7557opendoar:2022-02-11T00:00Jornal de Pediatria (Online) - Sociedade Brasileira de Pediatria (SBP)false |
dc.title.none.fl_str_mv |
Intra- and interobserver agreement of histopathological findings in pediatric patients with eosinophilic esophagitis |
title |
Intra- and interobserver agreement of histopathological findings in pediatric patients with eosinophilic esophagitis |
spellingShingle |
Intra- and interobserver agreement of histopathological findings in pediatric patients with eosinophilic esophagitis Vieira,Mário César Eosinophilic esophagitis Endoscopy Histology Observer variation |
title_short |
Intra- and interobserver agreement of histopathological findings in pediatric patients with eosinophilic esophagitis |
title_full |
Intra- and interobserver agreement of histopathological findings in pediatric patients with eosinophilic esophagitis |
title_fullStr |
Intra- and interobserver agreement of histopathological findings in pediatric patients with eosinophilic esophagitis |
title_full_unstemmed |
Intra- and interobserver agreement of histopathological findings in pediatric patients with eosinophilic esophagitis |
title_sort |
Intra- and interobserver agreement of histopathological findings in pediatric patients with eosinophilic esophagitis |
author |
Vieira,Mário César |
author_facet |
Vieira,Mário César Gugelmin,Elizabeth Schneider Percicote,Ana Paula Ribeiro,Melyssa Grignet Miranda,Renata Augusta de Vieira,Gabriela Guimãraes Ribeiro,Luciana Bandeira Mendez Truppel,Sabine Krüger Rosário Filho,Nelson Augusto |
author_role |
author |
author2 |
Gugelmin,Elizabeth Schneider Percicote,Ana Paula Ribeiro,Melyssa Grignet Miranda,Renata Augusta de Vieira,Gabriela Guimãraes Ribeiro,Luciana Bandeira Mendez Truppel,Sabine Krüger Rosário Filho,Nelson Augusto |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Vieira,Mário César Gugelmin,Elizabeth Schneider Percicote,Ana Paula Ribeiro,Melyssa Grignet Miranda,Renata Augusta de Vieira,Gabriela Guimãraes Ribeiro,Luciana Bandeira Mendez Truppel,Sabine Krüger Rosário Filho,Nelson Augusto |
dc.subject.por.fl_str_mv |
Eosinophilic esophagitis Endoscopy Histology Observer variation |
topic |
Eosinophilic esophagitis Endoscopy Histology Observer variation |
description |
Abstract Objective: To assess intra- and interobserver agreement among non-expert pathologists in identifying features of the eosinophilic esophagitis histologic scoring system (EoEHSS) in pediatric patients. Patients and methods: The authors used 50 slides from patients (aged 1-15 years; 72% male) with EoE. EoEHSS evaluates eosinophilic inflammation and other features including epithelial basal zone hyperplasia, eosinophilic abscesses, eosinophil surface layering, dilated intercellular spaces, surface epithelial alteration, dyskeratotic epithelial cells, and lamina propria fibrosis. Grade and stage of abnormalities are scored using a 4-point scale (0 normal; 3 maximum change). Four pathologists determined EoEHSS findings on two occasions. Intra- and interobserver agreement was assessed using Kappa (κ) statistics and intra-class correlation coefficients. Results: Intra- and interobserver agreement for the identification of eosinophil counts ≥ 15/high power field (HPF) was excellent, however varied when assessing additional features of the EoEHSS. For the more experienced pathologist, agreement for most EoEHSS items and the composite scores was substantial to excellent. For the less experienced pathologists, intraobserver agreement ranged from absent to substantial for individual features and ranged from moderate to substantial for the composite scores. Conclusion: Most items of the EoEHSS had substantial to excellent reliability when assessed by a pathologist experienced in the diagnosis of EoE but presented lower repeatability among less experienced pathologists. These findings suggest that specific training of pathologists is required for the identification of EoEHSS characteristics beyond eosinophil count, as these features are considered useful in the evaluation of response to treatment and correlation with clinical manifestations and endoscopic findings. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-02-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0021-75572022000100026 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0021-75572022000100026 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1016/j.jped.2021.03.004 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Pediatria |
publisher.none.fl_str_mv |
Sociedade Brasileira de Pediatria |
dc.source.none.fl_str_mv |
Jornal de Pediatria v.98 n.1 2022 reponame:Jornal de Pediatria (Online) instname:Sociedade Brasileira de Pediatria (SBP) instacron:SBPE |
instname_str |
Sociedade Brasileira de Pediatria (SBP) |
instacron_str |
SBPE |
institution |
SBPE |
reponame_str |
Jornal de Pediatria (Online) |
collection |
Jornal de Pediatria (Online) |
repository.name.fl_str_mv |
Jornal de Pediatria (Online) - Sociedade Brasileira de Pediatria (SBP) |
repository.mail.fl_str_mv |
||jped@jped.com.br |
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1752122323210600448 |