TLR-2 and TLR-4 expression in monocytes of newborns with late-onset sepsis,
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Jornal de Pediatria (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0021-75572014000500472 |
Resumo: | Objective:To analyze toll-like receptor (TLR)-2 and TLR-4 expression in monocytes of newborns with late-onset sepsis.Methods:This prospective study included 27 full-term newborns aged 8 to 29 days, with clinical and laboratory diagnosis of late-onset sepsis. Ten newborns (37%) had positive cultures. Cytokines were measured by cytometric bead array in peripheral blood, while TLR-2, TLR-4 expression, and median fluorescence intensity (MFI) were determined by immunophenotyping peripheral whole blood monocytes, and were analyzed with a BD FACSDiva flow cytometer (Becton, Dickinson and Company, USA). A comparison was performed with healthy adults.Results:Microorganisms were identified in 37% of these septic newborns, and all of them had high levels of pro-inflammatory cytokines (IL-8, IL-6, IL-1β) and anti-inflammatory cytokine (IL-10) corroborating the inflammatory/septic process. In monocytes, the frequency of TLR-4 expression was higher in infected newborns (p = 0.01).Conclusion:This study investigated the innate immune response in septic newborns. Septic newborns that relied almost exclusively on the innate immune system showed little in vivo response at monocyte activation, suggesting impaired immune response and increased susceptibility to infection. |
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TLR-2 and TLR-4 expression in monocytes of newborns with late-onset sepsis,Sepsis/immunologyInnate immunityToll-like receptor 2Toll-like receptor 4NewbornMonocytesObjective:To analyze toll-like receptor (TLR)-2 and TLR-4 expression in monocytes of newborns with late-onset sepsis.Methods:This prospective study included 27 full-term newborns aged 8 to 29 days, with clinical and laboratory diagnosis of late-onset sepsis. Ten newborns (37%) had positive cultures. Cytokines were measured by cytometric bead array in peripheral blood, while TLR-2, TLR-4 expression, and median fluorescence intensity (MFI) were determined by immunophenotyping peripheral whole blood monocytes, and were analyzed with a BD FACSDiva flow cytometer (Becton, Dickinson and Company, USA). A comparison was performed with healthy adults.Results:Microorganisms were identified in 37% of these septic newborns, and all of them had high levels of pro-inflammatory cytokines (IL-8, IL-6, IL-1β) and anti-inflammatory cytokine (IL-10) corroborating the inflammatory/septic process. In monocytes, the frequency of TLR-4 expression was higher in infected newborns (p = 0.01).Conclusion:This study investigated the innate immune response in septic newborns. Septic newborns that relied almost exclusively on the innate immune system showed little in vivo response at monocyte activation, suggesting impaired immune response and increased susceptibility to infection.Sociedade Brasileira de Pediatria2014-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0021-75572014000500472Jornal de Pediatria v.90 n.5 2014reponame:Jornal de Pediatria (Online)instname:Sociedade Brasileira de Pediatria (SBP)instacron:SBPE10.1016/j.jped.2013.12.012info:eu-repo/semantics/openAccessRedondo,Ana C.C.Ceccon,Maria E.J.R.Silveira-Lessa,Ana L.Quinello,CamilaPalmeira,PatríciaCarvalho,Werther B.Carneiro-Sampaio,Magdaeng2015-08-28T00:00:00Zoai:scielo:S0021-75572014000500472Revistahttp://www.jped.com.br/https://old.scielo.br/oai/scielo-oai.php||jped@jped.com.br1678-47820021-7557opendoar:2015-08-28T00:00Jornal de Pediatria (Online) - Sociedade Brasileira de Pediatria (SBP)false |
dc.title.none.fl_str_mv |
TLR-2 and TLR-4 expression in monocytes of newborns with late-onset sepsis, |
title |
TLR-2 and TLR-4 expression in monocytes of newborns with late-onset sepsis, |
spellingShingle |
TLR-2 and TLR-4 expression in monocytes of newborns with late-onset sepsis, Redondo,Ana C.C. Sepsis/immunology Innate immunity Toll-like receptor 2 Toll-like receptor 4 Newborn Monocytes |
title_short |
TLR-2 and TLR-4 expression in monocytes of newborns with late-onset sepsis, |
title_full |
TLR-2 and TLR-4 expression in monocytes of newborns with late-onset sepsis, |
title_fullStr |
TLR-2 and TLR-4 expression in monocytes of newborns with late-onset sepsis, |
title_full_unstemmed |
TLR-2 and TLR-4 expression in monocytes of newborns with late-onset sepsis, |
title_sort |
TLR-2 and TLR-4 expression in monocytes of newborns with late-onset sepsis, |
author |
Redondo,Ana C.C. |
author_facet |
Redondo,Ana C.C. Ceccon,Maria E.J.R. Silveira-Lessa,Ana L. Quinello,Camila Palmeira,Patrícia Carvalho,Werther B. Carneiro-Sampaio,Magda |
author_role |
author |
author2 |
Ceccon,Maria E.J.R. Silveira-Lessa,Ana L. Quinello,Camila Palmeira,Patrícia Carvalho,Werther B. Carneiro-Sampaio,Magda |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Redondo,Ana C.C. Ceccon,Maria E.J.R. Silveira-Lessa,Ana L. Quinello,Camila Palmeira,Patrícia Carvalho,Werther B. Carneiro-Sampaio,Magda |
dc.subject.por.fl_str_mv |
Sepsis/immunology Innate immunity Toll-like receptor 2 Toll-like receptor 4 Newborn Monocytes |
topic |
Sepsis/immunology Innate immunity Toll-like receptor 2 Toll-like receptor 4 Newborn Monocytes |
description |
Objective:To analyze toll-like receptor (TLR)-2 and TLR-4 expression in monocytes of newborns with late-onset sepsis.Methods:This prospective study included 27 full-term newborns aged 8 to 29 days, with clinical and laboratory diagnosis of late-onset sepsis. Ten newborns (37%) had positive cultures. Cytokines were measured by cytometric bead array in peripheral blood, while TLR-2, TLR-4 expression, and median fluorescence intensity (MFI) were determined by immunophenotyping peripheral whole blood monocytes, and were analyzed with a BD FACSDiva flow cytometer (Becton, Dickinson and Company, USA). A comparison was performed with healthy adults.Results:Microorganisms were identified in 37% of these septic newborns, and all of them had high levels of pro-inflammatory cytokines (IL-8, IL-6, IL-1β) and anti-inflammatory cytokine (IL-10) corroborating the inflammatory/septic process. In monocytes, the frequency of TLR-4 expression was higher in infected newborns (p = 0.01).Conclusion:This study investigated the innate immune response in septic newborns. Septic newborns that relied almost exclusively on the innate immune system showed little in vivo response at monocyte activation, suggesting impaired immune response and increased susceptibility to infection. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0021-75572014000500472 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0021-75572014000500472 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1016/j.jped.2013.12.012 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Pediatria |
publisher.none.fl_str_mv |
Sociedade Brasileira de Pediatria |
dc.source.none.fl_str_mv |
Jornal de Pediatria v.90 n.5 2014 reponame:Jornal de Pediatria (Online) instname:Sociedade Brasileira de Pediatria (SBP) instacron:SBPE |
instname_str |
Sociedade Brasileira de Pediatria (SBP) |
instacron_str |
SBPE |
institution |
SBPE |
reponame_str |
Jornal de Pediatria (Online) |
collection |
Jornal de Pediatria (Online) |
repository.name.fl_str_mv |
Jornal de Pediatria (Online) - Sociedade Brasileira de Pediatria (SBP) |
repository.mail.fl_str_mv |
||jped@jped.com.br |
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1752122319993569280 |