TLR-2 and TLR-4 expression in monocytes of newborns with late-onset sepsis,

Detalhes bibliográficos
Autor(a) principal: Redondo,Ana C.C.
Data de Publicação: 2014
Outros Autores: Ceccon,Maria E.J.R., Silveira-Lessa,Ana L., Quinello,Camila, Palmeira,Patrícia, Carvalho,Werther B., Carneiro-Sampaio,Magda
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Jornal de Pediatria (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0021-75572014000500472
Resumo: Objective:To analyze toll-like receptor (TLR)-2 and TLR-4 expression in monocytes of newborns with late-onset sepsis.Methods:This prospective study included 27 full-term newborns aged 8 to 29 days, with clinical and laboratory diagnosis of late-onset sepsis. Ten newborns (37%) had positive cultures. Cytokines were measured by cytometric bead array in peripheral blood, while TLR-2, TLR-4 expression, and median fluorescence intensity (MFI) were determined by immunophenotyping peripheral whole blood monocytes, and were analyzed with a BD FACSDiva flow cytometer (Becton, Dickinson and Company, USA). A comparison was performed with healthy adults.Results:Microorganisms were identified in 37% of these septic newborns, and all of them had high levels of pro-inflammatory cytokines (IL-8, IL-6, IL-1β) and anti-inflammatory cytokine (IL-10) corroborating the inflammatory/septic process. In monocytes, the frequency of TLR-4 expression was higher in infected newborns (p = 0.01).Conclusion:This study investigated the innate immune response in septic newborns. Septic newborns that relied almost exclusively on the innate immune system showed little in vivo response at monocyte activation, suggesting impaired immune response and increased susceptibility to infection.
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spelling TLR-2 and TLR-4 expression in monocytes of newborns with late-onset sepsis,Sepsis/immunologyInnate immunityToll-like receptor 2Toll-like receptor 4NewbornMonocytesObjective:To analyze toll-like receptor (TLR)-2 and TLR-4 expression in monocytes of newborns with late-onset sepsis.Methods:This prospective study included 27 full-term newborns aged 8 to 29 days, with clinical and laboratory diagnosis of late-onset sepsis. Ten newborns (37%) had positive cultures. Cytokines were measured by cytometric bead array in peripheral blood, while TLR-2, TLR-4 expression, and median fluorescence intensity (MFI) were determined by immunophenotyping peripheral whole blood monocytes, and were analyzed with a BD FACSDiva flow cytometer (Becton, Dickinson and Company, USA). A comparison was performed with healthy adults.Results:Microorganisms were identified in 37% of these septic newborns, and all of them had high levels of pro-inflammatory cytokines (IL-8, IL-6, IL-1β) and anti-inflammatory cytokine (IL-10) corroborating the inflammatory/septic process. In monocytes, the frequency of TLR-4 expression was higher in infected newborns (p = 0.01).Conclusion:This study investigated the innate immune response in septic newborns. Septic newborns that relied almost exclusively on the innate immune system showed little in vivo response at monocyte activation, suggesting impaired immune response and increased susceptibility to infection.Sociedade Brasileira de Pediatria2014-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0021-75572014000500472Jornal de Pediatria v.90 n.5 2014reponame:Jornal de Pediatria (Online)instname:Sociedade Brasileira de Pediatria (SBP)instacron:SBPE10.1016/j.jped.2013.12.012info:eu-repo/semantics/openAccessRedondo,Ana C.C.Ceccon,Maria E.J.R.Silveira-Lessa,Ana L.Quinello,CamilaPalmeira,PatríciaCarvalho,Werther B.Carneiro-Sampaio,Magdaeng2015-08-28T00:00:00Zoai:scielo:S0021-75572014000500472Revistahttp://www.jped.com.br/https://old.scielo.br/oai/scielo-oai.php||jped@jped.com.br1678-47820021-7557opendoar:2015-08-28T00:00Jornal de Pediatria (Online) - Sociedade Brasileira de Pediatria (SBP)false
dc.title.none.fl_str_mv TLR-2 and TLR-4 expression in monocytes of newborns with late-onset sepsis,
title TLR-2 and TLR-4 expression in monocytes of newborns with late-onset sepsis,
spellingShingle TLR-2 and TLR-4 expression in monocytes of newborns with late-onset sepsis,
Redondo,Ana C.C.
Sepsis/immunology
Innate immunity
Toll-like receptor 2
Toll-like receptor 4
Newborn
Monocytes
title_short TLR-2 and TLR-4 expression in monocytes of newborns with late-onset sepsis,
title_full TLR-2 and TLR-4 expression in monocytes of newborns with late-onset sepsis,
title_fullStr TLR-2 and TLR-4 expression in monocytes of newborns with late-onset sepsis,
title_full_unstemmed TLR-2 and TLR-4 expression in monocytes of newborns with late-onset sepsis,
title_sort TLR-2 and TLR-4 expression in monocytes of newborns with late-onset sepsis,
author Redondo,Ana C.C.
author_facet Redondo,Ana C.C.
Ceccon,Maria E.J.R.
Silveira-Lessa,Ana L.
Quinello,Camila
Palmeira,Patrícia
Carvalho,Werther B.
Carneiro-Sampaio,Magda
author_role author
author2 Ceccon,Maria E.J.R.
Silveira-Lessa,Ana L.
Quinello,Camila
Palmeira,Patrícia
Carvalho,Werther B.
Carneiro-Sampaio,Magda
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Redondo,Ana C.C.
Ceccon,Maria E.J.R.
Silveira-Lessa,Ana L.
Quinello,Camila
Palmeira,Patrícia
Carvalho,Werther B.
Carneiro-Sampaio,Magda
dc.subject.por.fl_str_mv Sepsis/immunology
Innate immunity
Toll-like receptor 2
Toll-like receptor 4
Newborn
Monocytes
topic Sepsis/immunology
Innate immunity
Toll-like receptor 2
Toll-like receptor 4
Newborn
Monocytes
description Objective:To analyze toll-like receptor (TLR)-2 and TLR-4 expression in monocytes of newborns with late-onset sepsis.Methods:This prospective study included 27 full-term newborns aged 8 to 29 days, with clinical and laboratory diagnosis of late-onset sepsis. Ten newborns (37%) had positive cultures. Cytokines were measured by cytometric bead array in peripheral blood, while TLR-2, TLR-4 expression, and median fluorescence intensity (MFI) were determined by immunophenotyping peripheral whole blood monocytes, and were analyzed with a BD FACSDiva flow cytometer (Becton, Dickinson and Company, USA). A comparison was performed with healthy adults.Results:Microorganisms were identified in 37% of these septic newborns, and all of them had high levels of pro-inflammatory cytokines (IL-8, IL-6, IL-1β) and anti-inflammatory cytokine (IL-10) corroborating the inflammatory/septic process. In monocytes, the frequency of TLR-4 expression was higher in infected newborns (p = 0.01).Conclusion:This study investigated the innate immune response in septic newborns. Septic newborns that relied almost exclusively on the innate immune system showed little in vivo response at monocyte activation, suggesting impaired immune response and increased susceptibility to infection.
publishDate 2014
dc.date.none.fl_str_mv 2014-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0021-75572014000500472
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0021-75572014000500472
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1016/j.jped.2013.12.012
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Pediatria
publisher.none.fl_str_mv Sociedade Brasileira de Pediatria
dc.source.none.fl_str_mv Jornal de Pediatria v.90 n.5 2014
reponame:Jornal de Pediatria (Online)
instname:Sociedade Brasileira de Pediatria (SBP)
instacron:SBPE
instname_str Sociedade Brasileira de Pediatria (SBP)
instacron_str SBPE
institution SBPE
reponame_str Jornal de Pediatria (Online)
collection Jornal de Pediatria (Online)
repository.name.fl_str_mv Jornal de Pediatria (Online) - Sociedade Brasileira de Pediatria (SBP)
repository.mail.fl_str_mv ||jped@jped.com.br
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