Solasodine accumulation in regenerated plants of Solanum torvum Sw

• Autor(a) principal: Moreira,C.B
• Data de Publicação: 2010
• Outros Autores: Lima,S.S, Esquibel,M.A, Sato,A
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Revista brasileira de plantas medicinais (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-05722010000100011
• topic: Solanaceae; "Jurubeba"; auxin; cytokinin; steroidal alkaloid

A nodal segment culture was developed in order to assess Solanum torvum Sw. regeneration and solasodine levels. The influence of auxins (indoleacetic acid, 1-Naphthaleneacetic acid) and benzyl adenine on S. torvum growth in micropropagation was investigated. A nodal segment culture was initiated with seeds germinated in MS basal medium added of GA3 and grown in different concentrations of IAA, IAA + BAP and NAA + BAP. Sixty-day-old plants from the in vitro culture were collected, frozen and lyophilized; then, the methyl orange method was used to quantify solasodine for the spectrophotometric assay. The best results regarding plant regeneration and solasodine accumulation were obtained by using the MS basal medium without addition of plant growth regulators; however, there was great production of calluses presenting friable bases. Based on these results, cell cultures can be initiated from such calluses with application of other auxins and cytokinins to enhance solasodine production, besides different elicitors, light intensities and sucrose concentrations.