In vitro effect of Acanthospermum australe (Asteraceae) extracts on Acanthamoeba polyphaga trophozoites
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista brasileira de plantas medicinais (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-05722013000400016 |
Resumo: | Acanthamoeba is a free-living protozoan widely distributed in the environment, occurring in vegetative trophozoite and resistance cyst stages during its life cycle. It constitutes an etiological agent of Acanthamoeba keratitis, a disease that may cause severe ocular inflammation and blindness. New drugs can be developed from molecules found in plants and thus help in its difficult treatment. Acanthospermum australe (Asteraceae), a plant used in folk medicine, had its effect tested on Acanthamoeba polyphaga. Aqueous and ethanolic extracts of A. austral were obtained from aerial parts for infusion and static maceration, respectively. Concentrations of 10, 5, 2.5, 1.25 and 0.625 mg/ml of the extract were tested against Acanthamoeba polyphaga trophozoites. The cytotoxic effect of the extracts was tested in mammalian cells using the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. RESULTS: The 10 mg/ml concentration of ethanolic extract was lethal to 100% of the A. polyphaga trophozoites in 24 h and both extracts presented cytotoxic effect against mammalian cells. These findings suggest that the A. austral ethanolic extract may have compounds with relevance to the development of new amoebicidal drugs. |
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In vitro effect of Acanthospermum australe (Asteraceae) extracts on Acanthamoeba polyphaga trophozoitesAcanthamoeba polyphagaamoebicidal activityAcanthospermum australeAcanthamoeba is a free-living protozoan widely distributed in the environment, occurring in vegetative trophozoite and resistance cyst stages during its life cycle. It constitutes an etiological agent of Acanthamoeba keratitis, a disease that may cause severe ocular inflammation and blindness. New drugs can be developed from molecules found in plants and thus help in its difficult treatment. Acanthospermum australe (Asteraceae), a plant used in folk medicine, had its effect tested on Acanthamoeba polyphaga. Aqueous and ethanolic extracts of A. austral were obtained from aerial parts for infusion and static maceration, respectively. Concentrations of 10, 5, 2.5, 1.25 and 0.625 mg/ml of the extract were tested against Acanthamoeba polyphaga trophozoites. The cytotoxic effect of the extracts was tested in mammalian cells using the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. RESULTS: The 10 mg/ml concentration of ethanolic extract was lethal to 100% of the A. polyphaga trophozoites in 24 h and both extracts presented cytotoxic effect against mammalian cells. These findings suggest that the A. austral ethanolic extract may have compounds with relevance to the development of new amoebicidal drugs.Sociedade Brasileira de Plantas Medicinais2013-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-05722013000400016Revista Brasileira de Plantas Medicinais v.15 n.4 2013reponame:Revista brasileira de plantas medicinais (Online)instname:Universidade Estadual Paulista (UNESP)instacron:SBPM10.1590/S1516-05722013000400016info:eu-repo/semantics/openAccessCastro,LCSauter,IPEthur,EMKauffmann,CDall'agnol,RSouza,JCibulski,SPMuniz,AWWeidlich,LLohmann,PMRoehe,PMGermani,JCRott,MBVand Der Sand,STeng2013-12-02T00:00:00Zoai:scielo:S1516-05722013000400016Revistahttp://www.scielo.br/scielo.php?script=sci_serial&pid=1516-0572&lng=en&nrm=isoPUBhttps://old.scielo.br/oai/scielo-oai.php||rbpm.sbpm@gmail.com1983-084X1516-0572opendoar:2013-12-02T00:00Revista brasileira de plantas medicinais (Online) - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
In vitro effect of Acanthospermum australe (Asteraceae) extracts on Acanthamoeba polyphaga trophozoites |
title |
In vitro effect of Acanthospermum australe (Asteraceae) extracts on Acanthamoeba polyphaga trophozoites |
spellingShingle |
In vitro effect of Acanthospermum australe (Asteraceae) extracts on Acanthamoeba polyphaga trophozoites Castro,LC Acanthamoeba polyphaga amoebicidal activity Acanthospermum australe |
title_short |
In vitro effect of Acanthospermum australe (Asteraceae) extracts on Acanthamoeba polyphaga trophozoites |
title_full |
In vitro effect of Acanthospermum australe (Asteraceae) extracts on Acanthamoeba polyphaga trophozoites |
title_fullStr |
In vitro effect of Acanthospermum australe (Asteraceae) extracts on Acanthamoeba polyphaga trophozoites |
title_full_unstemmed |
In vitro effect of Acanthospermum australe (Asteraceae) extracts on Acanthamoeba polyphaga trophozoites |
title_sort |
In vitro effect of Acanthospermum australe (Asteraceae) extracts on Acanthamoeba polyphaga trophozoites |
author |
Castro,LC |
author_facet |
Castro,LC Sauter,IP Ethur,EM Kauffmann,C Dall'agnol,R Souza,J Cibulski,SP Muniz,AW Weidlich,L Lohmann,PM Roehe,PM Germani,JC Rott,MB Vand Der Sand,ST |
author_role |
author |
author2 |
Sauter,IP Ethur,EM Kauffmann,C Dall'agnol,R Souza,J Cibulski,SP Muniz,AW Weidlich,L Lohmann,PM Roehe,PM Germani,JC Rott,MB Vand Der Sand,ST |
author2_role |
author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Castro,LC Sauter,IP Ethur,EM Kauffmann,C Dall'agnol,R Souza,J Cibulski,SP Muniz,AW Weidlich,L Lohmann,PM Roehe,PM Germani,JC Rott,MB Vand Der Sand,ST |
dc.subject.por.fl_str_mv |
Acanthamoeba polyphaga amoebicidal activity Acanthospermum australe |
topic |
Acanthamoeba polyphaga amoebicidal activity Acanthospermum australe |
description |
Acanthamoeba is a free-living protozoan widely distributed in the environment, occurring in vegetative trophozoite and resistance cyst stages during its life cycle. It constitutes an etiological agent of Acanthamoeba keratitis, a disease that may cause severe ocular inflammation and blindness. New drugs can be developed from molecules found in plants and thus help in its difficult treatment. Acanthospermum australe (Asteraceae), a plant used in folk medicine, had its effect tested on Acanthamoeba polyphaga. Aqueous and ethanolic extracts of A. austral were obtained from aerial parts for infusion and static maceration, respectively. Concentrations of 10, 5, 2.5, 1.25 and 0.625 mg/ml of the extract were tested against Acanthamoeba polyphaga trophozoites. The cytotoxic effect of the extracts was tested in mammalian cells using the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. RESULTS: The 10 mg/ml concentration of ethanolic extract was lethal to 100% of the A. polyphaga trophozoites in 24 h and both extracts presented cytotoxic effect against mammalian cells. These findings suggest that the A. austral ethanolic extract may have compounds with relevance to the development of new amoebicidal drugs. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-05722013000400016 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-05722013000400016 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1516-05722013000400016 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Plantas Medicinais |
publisher.none.fl_str_mv |
Sociedade Brasileira de Plantas Medicinais |
dc.source.none.fl_str_mv |
Revista Brasileira de Plantas Medicinais v.15 n.4 2013 reponame:Revista brasileira de plantas medicinais (Online) instname:Universidade Estadual Paulista (UNESP) instacron:SBPM |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
SBPM |
institution |
SBPM |
reponame_str |
Revista brasileira de plantas medicinais (Online) |
collection |
Revista brasileira de plantas medicinais (Online) |
repository.name.fl_str_mv |
Revista brasileira de plantas medicinais (Online) - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
||rbpm.sbpm@gmail.com |
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1750318028428410880 |