Chitosan-collagen biomembrane embedded with calcium-aluminate enhances dentinogenic potential of pulp cells
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Oral Research |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242016000100240 |
Resumo: | Abstract The development of biomaterials capable of driving dental pulp stem cell differentiation into odontoblast-like cells able to secrete reparative dentin is the goal of current conservative dentistry. In the present investigation, a biomembrane (BM) composed of a chitosan/collagen matrix embedded with calcium-aluminate microparticles was tested. The BM was produced by mixing collagen gel with a chitosan solution (2:1), and then adding bioactive calcium-aluminate cement as the mineral phase. An inert material (polystyrene) was used as the negative control. Human dental pulp cells were seeded onto the surface of certain materials, and the cytocompatibility was evaluated by cell proliferation and cell morphology, assessed after 1, 7, 14 and 28 days in culture. The odontoblastic differentiation was evaluated by measuring alkaline phosphatase (ALP) activity, total protein production, gene expression of DMP-1/DSPP and mineralized nodule deposition. The pulp cells were able to attach onto the BM surface and spread, displaying a faster proliferative rate at initial periods than that of the control cells. The BM also acted on the cells to induce more intense ALP activity, protein production at 14 days, and higher gene expression of DSPP and DMP-1 at 28 days, leading to the deposition of about five times more mineralized matrix than the cells in the control group. Therefore, the experimental biomembrane induced the differentiation of pulp cells into odontoblast-like cells featuring a highly secretory phenotype. This innovative bioactive material can drive other protocols for dental pulp exposure treatment by inducing the regeneration of dentin tissue mediated by resident cells. |
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Brazilian Oral Research |
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Chitosan-collagen biomembrane embedded with calcium-aluminate enhances dentinogenic potential of pulp cellsDental PulpBiocompatible MaterialsTissue EngineeringStem CellsDentinAbstract The development of biomaterials capable of driving dental pulp stem cell differentiation into odontoblast-like cells able to secrete reparative dentin is the goal of current conservative dentistry. In the present investigation, a biomembrane (BM) composed of a chitosan/collagen matrix embedded with calcium-aluminate microparticles was tested. The BM was produced by mixing collagen gel with a chitosan solution (2:1), and then adding bioactive calcium-aluminate cement as the mineral phase. An inert material (polystyrene) was used as the negative control. Human dental pulp cells were seeded onto the surface of certain materials, and the cytocompatibility was evaluated by cell proliferation and cell morphology, assessed after 1, 7, 14 and 28 days in culture. The odontoblastic differentiation was evaluated by measuring alkaline phosphatase (ALP) activity, total protein production, gene expression of DMP-1/DSPP and mineralized nodule deposition. The pulp cells were able to attach onto the BM surface and spread, displaying a faster proliferative rate at initial periods than that of the control cells. The BM also acted on the cells to induce more intense ALP activity, protein production at 14 days, and higher gene expression of DSPP and DMP-1 at 28 days, leading to the deposition of about five times more mineralized matrix than the cells in the control group. Therefore, the experimental biomembrane induced the differentiation of pulp cells into odontoblast-like cells featuring a highly secretory phenotype. This innovative bioactive material can drive other protocols for dental pulp exposure treatment by inducing the regeneration of dentin tissue mediated by resident cells.Sociedade Brasileira de Pesquisa Odontológica - SBPqO2016-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242016000100240Brazilian Oral Research v.30 n.1 2016reponame:Brazilian Oral Researchinstname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO)instacron:SBPQO10.1590/1807-3107BOR-2016.vol30.0054info:eu-repo/semantics/openAccessSOARES,Diana GabrielaROSSETO,Hebert LuísBASSO,Fernanda GonçalvesSCHEFFEL,Débora SallesHEBLING,JosimeriCOSTA,Carlos Alberto de Souzaeng2016-04-19T00:00:00Zoai:scielo:S1806-83242016000100240Revistahttps://www.scielo.br/j/bor/https://old.scielo.br/oai/scielo-oai.phppob@edu.usp.br||bor@sbpqo.org.br1807-31071806-8324opendoar:2016-04-19T00:00Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO)false |
dc.title.none.fl_str_mv |
Chitosan-collagen biomembrane embedded with calcium-aluminate enhances dentinogenic potential of pulp cells |
title |
Chitosan-collagen biomembrane embedded with calcium-aluminate enhances dentinogenic potential of pulp cells |
spellingShingle |
Chitosan-collagen biomembrane embedded with calcium-aluminate enhances dentinogenic potential of pulp cells SOARES,Diana Gabriela Dental Pulp Biocompatible Materials Tissue Engineering Stem Cells Dentin |
title_short |
Chitosan-collagen biomembrane embedded with calcium-aluminate enhances dentinogenic potential of pulp cells |
title_full |
Chitosan-collagen biomembrane embedded with calcium-aluminate enhances dentinogenic potential of pulp cells |
title_fullStr |
Chitosan-collagen biomembrane embedded with calcium-aluminate enhances dentinogenic potential of pulp cells |
title_full_unstemmed |
Chitosan-collagen biomembrane embedded with calcium-aluminate enhances dentinogenic potential of pulp cells |
title_sort |
Chitosan-collagen biomembrane embedded with calcium-aluminate enhances dentinogenic potential of pulp cells |
author |
SOARES,Diana Gabriela |
author_facet |
SOARES,Diana Gabriela ROSSETO,Hebert Luís BASSO,Fernanda Gonçalves SCHEFFEL,Débora Salles HEBLING,Josimeri COSTA,Carlos Alberto de Souza |
author_role |
author |
author2 |
ROSSETO,Hebert Luís BASSO,Fernanda Gonçalves SCHEFFEL,Débora Salles HEBLING,Josimeri COSTA,Carlos Alberto de Souza |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
SOARES,Diana Gabriela ROSSETO,Hebert Luís BASSO,Fernanda Gonçalves SCHEFFEL,Débora Salles HEBLING,Josimeri COSTA,Carlos Alberto de Souza |
dc.subject.por.fl_str_mv |
Dental Pulp Biocompatible Materials Tissue Engineering Stem Cells Dentin |
topic |
Dental Pulp Biocompatible Materials Tissue Engineering Stem Cells Dentin |
description |
Abstract The development of biomaterials capable of driving dental pulp stem cell differentiation into odontoblast-like cells able to secrete reparative dentin is the goal of current conservative dentistry. In the present investigation, a biomembrane (BM) composed of a chitosan/collagen matrix embedded with calcium-aluminate microparticles was tested. The BM was produced by mixing collagen gel with a chitosan solution (2:1), and then adding bioactive calcium-aluminate cement as the mineral phase. An inert material (polystyrene) was used as the negative control. Human dental pulp cells were seeded onto the surface of certain materials, and the cytocompatibility was evaluated by cell proliferation and cell morphology, assessed after 1, 7, 14 and 28 days in culture. The odontoblastic differentiation was evaluated by measuring alkaline phosphatase (ALP) activity, total protein production, gene expression of DMP-1/DSPP and mineralized nodule deposition. The pulp cells were able to attach onto the BM surface and spread, displaying a faster proliferative rate at initial periods than that of the control cells. The BM also acted on the cells to induce more intense ALP activity, protein production at 14 days, and higher gene expression of DSPP and DMP-1 at 28 days, leading to the deposition of about five times more mineralized matrix than the cells in the control group. Therefore, the experimental biomembrane induced the differentiation of pulp cells into odontoblast-like cells featuring a highly secretory phenotype. This innovative bioactive material can drive other protocols for dental pulp exposure treatment by inducing the regeneration of dentin tissue mediated by resident cells. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242016000100240 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242016000100240 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1807-3107BOR-2016.vol30.0054 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica - SBPqO |
publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica - SBPqO |
dc.source.none.fl_str_mv |
Brazilian Oral Research v.30 n.1 2016 reponame:Brazilian Oral Research instname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO) instacron:SBPQO |
instname_str |
Sociedade Brasileira de Pesquisa Odontológica (SBPqO) |
instacron_str |
SBPQO |
institution |
SBPQO |
reponame_str |
Brazilian Oral Research |
collection |
Brazilian Oral Research |
repository.name.fl_str_mv |
Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO) |
repository.mail.fl_str_mv |
pob@edu.usp.br||bor@sbpqo.org.br |
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1750318324722434048 |