In vitro evaluation of Eugenia dysenterica in primary culture of human gingival fibroblast cells

Detalhes bibliográficos
Autor(a) principal: Costa,Cláudio Rodrigues Rezende
Data de Publicação: 2019
Outros Autores: Amorim,Bruna Rabelo, Silva,Sandra Márcia Mazutti da, Acevedo,Ana Carolina, Magalhães,Pérola de Oliveira, Guerra,Eliete Neves Silva
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Oral Research
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242019000100225
Resumo: Abstract: Eugenia dysenterica is a Brazilian tree investigated for its properties and bioactive compounds, which are believed to have both pharmacological and phytochemical therapeutic effects. The leaves of this tree contain tannins, flavonoids, terpenes, and saponins, with reportedly beneficial effects to the human body. Despite these therapeutic applications, its effects have never been tested on oral tissues. Therefore, the aim of the present study was to evaluate the cytotoxic and antioxidant effects and the anti-inflammatory and repair properties of the acetone fraction of E. dysenterica on primary culture of human gingival fibroblasts and on the immortalized murine macrophage cell line (RAW 264.7). For this purpose, a metabolic activity assay, a wound healing assay, a nitric oxide assay, and RT-qPCR were performed. The assays revealed a cytoprotective effect of this plant, suggested by the increase in the expression of SOD1 and NRF2. An antioxidant potential effect was observed in the DPPH• assay. However, the fraction of E. dysenterica did not show anti-inflammatory activity. In conclusion, Eugenia dysenterica may promote cytoprotection when associated with chlorhexidine digluconate because of its antioxidant effect. However, additional studies are necessary on other human dental tissues using other parts of the plant in order to develop a possible mouthwash to assist patients with oral disorders.
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spelling In vitro evaluation of Eugenia dysenterica in primary culture of human gingival fibroblast cellsEugeniaFibroblastsAntioxidantsChlorhexidineAbstract: Eugenia dysenterica is a Brazilian tree investigated for its properties and bioactive compounds, which are believed to have both pharmacological and phytochemical therapeutic effects. The leaves of this tree contain tannins, flavonoids, terpenes, and saponins, with reportedly beneficial effects to the human body. Despite these therapeutic applications, its effects have never been tested on oral tissues. Therefore, the aim of the present study was to evaluate the cytotoxic and antioxidant effects and the anti-inflammatory and repair properties of the acetone fraction of E. dysenterica on primary culture of human gingival fibroblasts and on the immortalized murine macrophage cell line (RAW 264.7). For this purpose, a metabolic activity assay, a wound healing assay, a nitric oxide assay, and RT-qPCR were performed. The assays revealed a cytoprotective effect of this plant, suggested by the increase in the expression of SOD1 and NRF2. An antioxidant potential effect was observed in the DPPH• assay. However, the fraction of E. dysenterica did not show anti-inflammatory activity. In conclusion, Eugenia dysenterica may promote cytoprotection when associated with chlorhexidine digluconate because of its antioxidant effect. However, additional studies are necessary on other human dental tissues using other parts of the plant in order to develop a possible mouthwash to assist patients with oral disorders.Sociedade Brasileira de Pesquisa Odontológica - SBPqO2019-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242019000100225Brazilian Oral Research v.33 2019reponame:Brazilian Oral Researchinstname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO)instacron:SBPQO10.1590/1807-3107bor-2019.vol33.0035info:eu-repo/semantics/openAccessCosta,Cláudio Rodrigues RezendeAmorim,Bruna RabeloSilva,Sandra Márcia Mazutti daAcevedo,Ana CarolinaMagalhães,Pérola de OliveiraGuerra,Eliete Neves Silvaeng2019-04-18T00:00:00Zoai:scielo:S1806-83242019000100225Revistahttps://www.scielo.br/j/bor/https://old.scielo.br/oai/scielo-oai.phppob@edu.usp.br||bor@sbpqo.org.br1807-31071806-8324opendoar:2019-04-18T00:00Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO)false
dc.title.none.fl_str_mv In vitro evaluation of Eugenia dysenterica in primary culture of human gingival fibroblast cells
title In vitro evaluation of Eugenia dysenterica in primary culture of human gingival fibroblast cells
spellingShingle In vitro evaluation of Eugenia dysenterica in primary culture of human gingival fibroblast cells
Costa,Cláudio Rodrigues Rezende
Eugenia
Fibroblasts
Antioxidants
Chlorhexidine
title_short In vitro evaluation of Eugenia dysenterica in primary culture of human gingival fibroblast cells
title_full In vitro evaluation of Eugenia dysenterica in primary culture of human gingival fibroblast cells
title_fullStr In vitro evaluation of Eugenia dysenterica in primary culture of human gingival fibroblast cells
title_full_unstemmed In vitro evaluation of Eugenia dysenterica in primary culture of human gingival fibroblast cells
title_sort In vitro evaluation of Eugenia dysenterica in primary culture of human gingival fibroblast cells
author Costa,Cláudio Rodrigues Rezende
author_facet Costa,Cláudio Rodrigues Rezende
Amorim,Bruna Rabelo
Silva,Sandra Márcia Mazutti da
Acevedo,Ana Carolina
Magalhães,Pérola de Oliveira
Guerra,Eliete Neves Silva
author_role author
author2 Amorim,Bruna Rabelo
Silva,Sandra Márcia Mazutti da
Acevedo,Ana Carolina
Magalhães,Pérola de Oliveira
Guerra,Eliete Neves Silva
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Costa,Cláudio Rodrigues Rezende
Amorim,Bruna Rabelo
Silva,Sandra Márcia Mazutti da
Acevedo,Ana Carolina
Magalhães,Pérola de Oliveira
Guerra,Eliete Neves Silva
dc.subject.por.fl_str_mv Eugenia
Fibroblasts
Antioxidants
Chlorhexidine
topic Eugenia
Fibroblasts
Antioxidants
Chlorhexidine
description Abstract: Eugenia dysenterica is a Brazilian tree investigated for its properties and bioactive compounds, which are believed to have both pharmacological and phytochemical therapeutic effects. The leaves of this tree contain tannins, flavonoids, terpenes, and saponins, with reportedly beneficial effects to the human body. Despite these therapeutic applications, its effects have never been tested on oral tissues. Therefore, the aim of the present study was to evaluate the cytotoxic and antioxidant effects and the anti-inflammatory and repair properties of the acetone fraction of E. dysenterica on primary culture of human gingival fibroblasts and on the immortalized murine macrophage cell line (RAW 264.7). For this purpose, a metabolic activity assay, a wound healing assay, a nitric oxide assay, and RT-qPCR were performed. The assays revealed a cytoprotective effect of this plant, suggested by the increase in the expression of SOD1 and NRF2. An antioxidant potential effect was observed in the DPPH• assay. However, the fraction of E. dysenterica did not show anti-inflammatory activity. In conclusion, Eugenia dysenterica may promote cytoprotection when associated with chlorhexidine digluconate because of its antioxidant effect. However, additional studies are necessary on other human dental tissues using other parts of the plant in order to develop a possible mouthwash to assist patients with oral disorders.
publishDate 2019
dc.date.none.fl_str_mv 2019-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242019000100225
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dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1807-3107bor-2019.vol33.0035
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Pesquisa Odontológica - SBPqO
publisher.none.fl_str_mv Sociedade Brasileira de Pesquisa Odontológica - SBPqO
dc.source.none.fl_str_mv Brazilian Oral Research v.33 2019
reponame:Brazilian Oral Research
instname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO)
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instname_str Sociedade Brasileira de Pesquisa Odontológica (SBPqO)
instacron_str SBPQO
institution SBPQO
reponame_str Brazilian Oral Research
collection Brazilian Oral Research
repository.name.fl_str_mv Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO)
repository.mail.fl_str_mv pob@edu.usp.br||bor@sbpqo.org.br
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